Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is s...Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is suitable for infecting lepidopteran pests,including Spodoptera litura and Helicoverpa armigera.This work aims to identify a suitable semi-solid fermentation medium to increase the conidia production of N.rileyi and enhance its biological control ability as mycoinsecticide.Research is carried out on broken rice,wheat,sorghum,and corn as substrates,both with and without nutrient addition.It also covers incubation under set conditions,colony harvesting,and conidial yield and germination determination using a haemocytometer.All the study was conducted using a completely randomized design(CRD)and a test of significance was set at p<0.05 using analysis of variance(ANOVA).This work aimed to establish the effect of various substrates on growth and conidia formation in N.rileyi.The highest conidia count of 10×10^(8)CFU/g on day 15.The result was obtained from BR+Nu followed by sorghum 7×10^(8)CFU/g,corn 7×10^(8)CFU/g and wheat 6.5×10^(8)CFU/g.Thus,post-drying,the highest corresponding viability was observed in the product containing BR+Nu–6.20×10^(8)CFU/g.Adding rice bran(BR+RB,150 g each)still increases conidial yield up to 14×10^(8) CFU/g at 15 DAI.These indings thus assert that broken rice and supplementation affect conidia yield and viability.展开更多
In this study, toxicity and control efficiency of three nicotine biopesticides against pomegranate aphids were determined via indoor toxicity test and field effica- cy test, aiming at screening the appropriate reagent...In this study, toxicity and control efficiency of three nicotine biopesticides against pomegranate aphids were determined via indoor toxicity test and field effica- cy test, aiming at screening the appropriate reagent for prevention and control of pomegranate aphids. The results of indoor toxicity test showed that LC^o of reagent 1, reagent 2 and reagent 3 reached 10 270, 12 810 and 13 040 mg/L, respectively. The results of field efficacy test showed that control efficacy of reagent 1, reagent 2 and reagent 3 reached 60.52%, 34.60% and 46.99%, respectively. Overall, reagent 1 （91% ultrafine powder of tobacco leaves, 3% silicone oil, 2% sasangua cake, 2% emulsifier, 2% ascorbic acid） exhibited the highest control efficiency against pomegranate aphids, which can be further popularized and utilized.展开更多
The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata lugens.In this study,the selective toxicity of seven commercial biopesticides for C.lividipennis was exami...The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata lugens.In this study,the selective toxicity of seven commercial biopesticides for C.lividipennis was examined under laboratory conditions; abamectin was the most selective to C.lividipennis,followed by matrine and azadirachtin.Veratridine,rotenone,Bacillus thuringiensis and Beauveria bassiana showed less selectivity for C.lividipennis.Subsequently,matrine,abamectin and azadirachtin were selected for sublethal assessments with respect to C.lividipennis due to their high toxicities to N.lugens.C.lividipennis treated with sublethal concentrations(LC10 and LC20) of the three biopesticides could distinguish volatiles released from healthy and N.lugens-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving miridbugs.Azadirachtin decreased the consumption capability of C.lividipennis when the densities of N.lugens were 20,30,40 and 50 insects per vial.Sublethal concentration treatment did not impact the pre-oviposition period or egg hatchability of C.lividipennis.However,the fecundity of C.lividipennis exposed to azadirachtin and abamectin increased by 27–41% compared to the untreated individuals.In summary,abamectin or matrine together with C.lividipennis could be considered an effective,sustainable pest management strategy for rice.展开更多
Biopesticides are derivatives of plants, microorganisms and insects. Substances from plants and animals have been used to manage diseases in crops, animals and humans. Reliance on nature to heal nature is a practise f...Biopesticides are derivatives of plants, microorganisms and insects. Substances from plants and animals have been used to manage diseases in crops, animals and humans. Reliance on nature to heal nature is a practise for many people around the world. Use of natural products was overtaken by synthetic chemicals due to their efficacy, reliability and quick knock down effect. However, synthetic pesticides have become a health hazards for humans and environment due to their toxicity and pollution. Biopesticides are potential alternatives to synthetic pesticides. Sources of biopesticides are readily available, easily biodegradable, exhibit various modes of action, are less expensive and have low toxicity to humans and non-target organisms. Neem, pyrethrum, cotton and tobacco are known sources of botanical pesticides and have already been commercialized. Other sources of botanical pesticides include garlic, euphorbia, citrus, pepper among others. Species of Trichoderma, Bacillus, Pseudomonas, Beauveria have been commercialized as microbial pesticides. Biopesticides are however faced with challenges of formulation, registration, commercialization, acceptance and adoption. This paper describes several aspects of biopesticide development, including but not limited to, their sources, production, formulation, commercialization, efficacy and role in sustainable agriculture.展开更多
[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzi...[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.展开更多
Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this r...Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this report, quantitative analysis of commercial biopesticides for the four insecticidal limonoids of neem extract-azadirachtin A, azadirachtin B, deacetylsalannin and salannin, was conducted through solid phase extraction method with lipophilic-hydrophilic balanced material. The recoveries of the four limonoids ranged from 80.5% to 105%, and their limit of quantitation ranged from 0.028 mg/L to 0.356 mg/L. On the five imported neem extracts, the total contents of the four bioactive limonoids extracted were from 321 mg/L to 5810 mg/L, but there were big variations in the relative composition of the limonoids. The total limonoidal concentrations in 23 commercial bio-pesticides made from neem showed from below LOQ to 7190 mg/L with significant differences in the relative composition. These differences determine the biopesticide’s efficacy on pests, therefore, tracking the active ingredients is necessary for the quality control of commercial bio-pesticides.展开更多
Successive cartridge clean-up method for the simultaneous determination of matrine and oxyma- trine in biopesticides containing Sophora flavescens extract was developed and validated by UPLC. The clean-up method was e...Successive cartridge clean-up method for the simultaneous determination of matrine and oxyma- trine in biopesticides containing Sophora flavescens extract was developed and validated by UPLC. The clean-up method was established with ENVI-Carb (0.5 g) and C18 SPE (0.5 g) cartridges for the bioactive alkaloid in biopesticides from S. flavescens, and the eluate was analyzed to quantify the matrine and oxymatrine by UPLC. The developed method was validated, and the recovery and LOQ of both materials were 105.0% and 103.6%, and 0.050 and 0.684 mg·kg-1, respectively. Of the twenty one samples, the total content of matrines were analyzed by using the developed method and the result showed the developed successive clean-up method could contribute to the manufacture and control of biopesticides including matrines, and can be ap- plied to the method development for the analysis of alkaloid materials in biopesticides.展开更多
Excessive application of mineral fertilizers and synthetic pesticides poses a substantial threat to the soil and water environment and food security.Organic fertilizer and biopesticides have gradually become essential...Excessive application of mineral fertilizers and synthetic pesticides poses a substantial threat to the soil and water environment and food security.Organic fertilizer and biopesticides have gradually become essential technology for reducing mineral fertilizer and pesticide inputs.In the process,the technical environment is critical for promoting farmer behavior related to the adoption of organic fertilizer and biopesticides.This paper analyzes the influence of the technical environment on farmer behavior related to the adoption of organic fertilizer and biopesticides based on a survey of 1282 farmers in Heilongjiang Province,China,using the bivariate probit model.The results indicate that(1)farmer behavior related to the adoption applying organic fertilizer and biopesticides were positively correlated;(2)the technical environment had a significant positive impact on farmer behavior related to the adoption of organic fertilizer and biopesticides;and(3)the technical environment had a heterogeneous effect across different groups of farmers.This research provides insights useful for promoting organic fertilizer and biopesticides to farmers.It can be helpful to bundle relevant environmental technologies,conduct technology training for farmers and strengthen the construction of rural information networks.展开更多
Cowpea, Vigna unguiculata L. Walp, is an important food grain legume in Niger facing production losses due to insect pests. This study aims to determine the efficiency of non-chemical methods for managing these pests....Cowpea, Vigna unguiculata L. Walp, is an important food grain legume in Niger facing production losses due to insect pests. This study aims to determine the efficiency of non-chemical methods for managing these pests. A trial was conducted during the 2020 and 2022 cropping seasons at the INRAN station in the Maradi region. A Fischer experimental design with 6 repetitions was used to compare 4 treatments: synthetic chemical pesticide;the entomopathogenic fungus Beauveria bassiana;aqueous extracts of neem seeds, and control. Observations were carried out every three days. The cowpea pod-sucking bug, pod borer, and thrips were the main insect pests recorded. In terms of effectiveness, the synthetic pesticide was the best treatment. It reduced insect pest densities by 71.35% to 90.40% in 2020 and by 35.11% to 42.13% in 2022. Grain yields varied between treatments. Neem seed extract followed the synthetic pesticide and significantly reduced insect infestations in both years. The synthetic pesticide and neem seed extract resulted in yields 3 to 5 times higher than the control treatment in 2020. By contrast, B. bassiana 115 and neem seed extract produced similar yields in 2022. Therefore, the results of this study showed that B. bassiana 115 and neem seed extract have insecticidal potential and could be used as an ecological alternative for managing cowpea insect pests in the Sahel.展开更多
Biopesticides are attracting interest as alternatives to conventional pesticides but without many of the non-target effects, promising a better record of safety and sustainability in pest control practices. In this ar...Biopesticides are attracting interest as alternatives to conventional pesticides but without many of the non-target effects, promising a better record of safety and sustainability in pest control practices. In this article we summarize and discuss the current status and future promise of biopesticides, including how biopesticides use may increase the quality and safety of the food supply.展开更多
Plants with bioactive properties are greatly useful in preventing and controlling blood-sucking and disease-vector invertebrates, particularly in developing countries and low-income communities. Their application is a...Plants with bioactive properties are greatly useful in preventing and controlling blood-sucking and disease-vector invertebrates, particularly in developing countries and low-income communities. Their application is a promising alternative to synthetic compounds whose use remains a health, environmental, and economic challenge. However, many are still unknown and unvalued, while others are becoming ignored and threatened. The main objective of this ethnobotanical study is to identify and characterize indigenous and locally grown plants against blood-sucking and disease-vector insects. Salient opportunities and challenges of using these plants are documented and discussed. Semi-structured interviews, using a prepared questionnaire, were conducted with 228 informants. The consensus index (CI) was calculated to analyze the reliability of the collected information. The identified 31 anti-insect plant species belong to 20 botanical families, four morphological categories, and six habitat types. They can be categorized as insecticidal plants (42% of the total), insect repellent (42% of the total), and both insecticidal and insect repellent (16% of the total). More than 54% of these are still abundant in the study area, while about 35.5% have become rare and difficultly accessible. Based on the numerical importance of related anti-insect plant species, the seven targeted blood-sucking insects range in the following decreasing order: Jiggers (16 species) > Fire Ants (9 species) > Flies (8 plants) > Mosquitoes (4 species) > Fleas (2 species) > Bedbugs (1 species) > lice (0 species). The three most commonly used plants, with the highest confirmation indices, are Tetradenia riparia (ICs = 0.712), Eucalyptus globulus subsp. maidenii (ICs = 0.302), and Solanum aculeastrum (ICs = 0.288). The antimicrobial role of many locally grown anti-insect plants and the multiple other associated valorization possibilities are ignored by most informants. Domesticating, propagating, protecting, and promoting the sustainable use of these plants would be an appropriate route for their conservation and continued availability.展开更多
Aromatic plant distillation produces residues rich in phenolic compounds known to be bioactive. In this context, residues from the hydrodistillation of Mentha piperita L., Cymbopogon citratus Stapf, and Eucalyptus cam...Aromatic plant distillation produces residues rich in phenolic compounds known to be bioactive. In this context, residues from the hydrodistillation of Mentha piperita L., Cymbopogon citratus Stapf, and Eucalyptus camaldulensis Dehnh were chemically and biologically analyzed. The ethanol percentages (70%, 50%, and 30%) were evaluated by determining antioxidant activity using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging, total phenolic content, and total flavonoid content. Antifungal activity was evaluated “in vitro” against Fusarium oxysporum, Alternaria alternata, Trametes versicolor, and Coniophora puteana using the diffusion method in malt-agar medium. Antitermite activity was also assessed against Reticulitermes flavipes using the most active extract with low ethanol concentrations (50% and 30%). The optimum ethanol concentration to extract phenolic compounds is 70% for TFC and 30% for TPC, and the antioxidant activity was 0.45 ± 0.01 mg/mL (IC50). The most active extracts were the ethanolic ones, particularly for Eucalyptus camaldulensis, presenting 100% inhibition against the mycelial growth of Coniophora puteana and Alternaria alternata at 1 mg/mL and 0.5 mg/mL, respectively. For antitermite activity, EE50 was the most effective, with a 24% termite survival rate and a degraded food supply surface of 28%. These data suggest the potential use of hydrodistillation residues for biopesticide development.展开更多
The present research aimed at producing a biopesticide with proper formulation of invert emulsion (water-in-oil type) and effective strain of?Trichoderma harzianum?then, testing it against pathogens of postharvest mol...The present research aimed at producing a biopesticide with proper formulation of invert emulsion (water-in-oil type) and effective strain of?Trichoderma harzianum?then, testing it against pathogens of postharvest mold infections(Botrytis cinerea?&?Penicillium expansum) on fresh fruits of apple and pear. The?proper formulation of invert emulsion (IE#3) used for biopesticide production has the following ingredients (100% w/w): soybean oil (28.50%), coconut oil (19.50%), oil-soluble emulsifier: Tween 20 (2.0%), glycerine (4.25%), water-soluble emulsifier:?dehymuls k (0.75%),?sterile distilled water (22.5%) and conidial suspension of the effective strain ofT. harzianum?“TrichoPAL1” in water (22.5%, concentration 1 ×?107?conidia/ml). Testing efficacy of the produced biopesticide has indicated a significant reduction in the disease lesion diameter of mold infections on wounded apple and pear fruits stored at?20℃?± 1℃?compared to the untreated fruits or control (reduction from up to 38.75 to about 7.50 mm, respectively, according to?the type of mold infections and fruit type). Also, the treatment with the produced biopesticide has resulted in?a long protection period from mold infections on wounded and un-wounded fresh fruits of apple and pear stored under controlled and semi-commercial conditions(up to 2.5 months according to the type of mold infections and fruit type). In conclusion, the overall results have demonstrated the effectiveness of produced biopesticide on stored fruits under controlled and semi-commercial conditions therefore,?it is recommended to test this effectiveness on marketed fruits stored under variable conditions before applying it at a large scale.展开更多
In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statisti...In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were conducted to study the control effect of biopesticide chitosan, biochemical compound Prochloraz-Chitosan and chemical pesticides difenoconazole and thifluzamide by field efficacy trials. The results showed that under serious occurrence of anthracnose in autumn greenhouse cowpea, when the 2% chJtosan AS （2 250 g/hm^2）, 46% Prochloraz-Chitosan EW （450 g/hm^2）, 10% difenoconazole WG （900 g/hm^2, CK） or 24% thifluzamide SC （360 ml/hm^2） were applied three times with an interval of 5-8 d, the control efficiency on day 7, 14 and 20 reached 83%, 78% and 73%, respectively. No significant difference was found in control efficiency among the four kinds of agents. These four kinds of agents, especially chitosan and Prochloraz-Chitosan, could be used as ideal agents for controlling anthracnose, as well as for modern agricultural demonstration zones, pollution-free agricultural products, green agricultural products and organic agricultural products,thereby meeting the develop- ment needs of ＇modern agriculture, green agriculture, organic agriculture and ecological agriculture.展开更多
The use of chemotherapeutants, antibiotics, and pesticides in aqua farms and non-adherence to scientific management practices have resulted in adverse impact on aquacultural production, serious outbreaks of diseases, ...The use of chemotherapeutants, antibiotics, and pesticides in aqua farms and non-adherence to scientific management practices have resulted in adverse impact on aquacultural production, serious outbreaks of diseases, development of drug resistance in microbes, and accumulation of antibiotics and pesticide residues in finfish and shellfish and environmental pollution. As a result, a need has been felt by the aquaculturists as well as aquatic animal health management professionals to find a suitable alternative therapy in place of antibiotics and chemotherapy. Phytotherapy has come to be recognized as a handy and viable alternative to chemotherapy, as it is economical, effective, non-resistance forming, renewable, eco-friendly and farmer-friendly. Although the use of medicinal plants is known to humanity since the dawn of human civilization for the treatment and control of human and animal diseases but its importance in combating finfish and shellfish diseases has been realized only recently. The phytotherapy of aquacultural diseases is in its infancy in most part of the world except in China to some extent. However, many important contributions in this field by different workers during the last quarter of the 20th century and early 21st century have shown encouraging results and opened new vistas in phytotherapy of aquatic animals. The present review crtically evaluates the present status of knowledge of phytotherapy in the world combating various aquacultural diseases, identifying the bottlenecks and suggests remedial measures.展开更多
Plant extracts have long been used in commercial agriculture as anti-microbial tools in food safety applications. These offer growers and agrobiologists many unique benefits which include their eco-friendliness. This ...Plant extracts have long been used in commercial agriculture as anti-microbial tools in food safety applications. These offer growers and agrobiologists many unique benefits which include their eco-friendliness. This work reviews the situation of Biofungicides reconnaissance in reference to fungal disease of cowpea. Twenty different pathogens were associated with various fungal diseases of cowpea and, only the species of Colletotrichum was found to have the virulence and propensity of afflicting a 100% infection on a single susceptible cowpea crop. Plant families under the affliction of Colletotrichum were analyzed. The different forms of botanicals so far availed for use as potential biofungicidal were identified. Eighteen plant families were found to represent the entire plants and plant materials agrobiologically screened within a range of thirteen years and found to habour large spectra of species containing substances of biofungicidal potentials. Current position in the use of Botanicals to combat agricultural pests and disease is 7% of the total cowpea disease management options.展开更多
Essential oils （EOs） from Rosemary, Rosmarinus officinalis （Lamiaceae）, Orange, Citrus sinensis （Rutaceae）, Lavandula, Lavandula angustifolia （Lamiaceae）, False Yellowhead, Dittrichia viscosa （Asteraceae） an...Essential oils （EOs） from Rosemary, Rosmarinus officinalis （Lamiaceae）, Orange, Citrus sinensis （Rutaceae）, Lavandula, Lavandula angustifolia （Lamiaceae）, False Yellowhead, Dittrichia viscosa （Asteraceae） and their major components were evaluated against root knot nematode, Meloidogyne incognita and Meloidogynejavanica. Second stage juveniles＇ （J2） paralysis and egg hatch inhibition were studied, while pulverized plant parts were tested for nematodes biological cycle arrest. All EOs paralyzed J2 and M. incognita were found more sensitive than M javanica with the EC50/4 days calculated at 250, 3,650 and 4,260 12g/mL for 1）. viscosa, L. angustifolia and R. officinalis, respectively. Similarly, all EOs inhibited nematodes egg hatch and D. viscosa exhibited the highest inhibition on egg hatch （100% inhibition at 5 μg/mL）. A significant influence of constituent terpenes （limonene, 1,8-cineole, linalool, camphor, L-borneol, caryophyllene oxide, β-eudesmol） dose and exposure time was indicated on egg hatch inhibition （56% to 100% at 500 μg/mL and 1,000 μg/mL）, while only β-eudesmol achieved paralysis of J2 and specifically against M. incognita （EC50/1d = 50μg/mL）. Interestingly, the most active botanical species arresting Meloidogyne spp. biological cycle in soil was C. sinensis （EC50 = 2 mg/g） and the most sensitive nematode species was M. javanica. The larvicidal and egg hatch inhibition activity holds promise towards the optimization of artificial terpene mixtures as novel and effective natural nematicides. Complex interactions of primary compounds and subsequent decomposition derivates compose efficacy profile of soil amendments.展开更多
The coconut mite, <i>Aceria</i> <i>guerreronis</i> Keifer (Acari: Eriophyidae), is a major pest of coconut plantations (<i>Cocos</i> <i>nucifera</i> L.) worldwide. Here,...The coconut mite, <i>Aceria</i> <i>guerreronis</i> Keifer (Acari: Eriophyidae), is a major pest of coconut plantations (<i>Cocos</i> <i>nucifera</i> L.) worldwide. Here, we conducted a bioguided<span style="font-family:;" "=""> </span><span style="font-family:;" "="">phytochemical approach using toxicity and repellency bioassays of nonpolar extract and its fractions of <i>Vitex</i> <i>gardneriana</i> Schauer (Lamiaceae) leaves to this pest. Nonpolar crude extract was fractionated by column chromatography using solvents with increased polarity and binary mixtures, resulting in five semipurified groups. The biomonitor</span><span style="font-family:;" "="">ing</span><span style="font-family:;" "=""> bioassay provided active fractions and led to the isolation and characterization of the <span>bioactive compound squalene, a biosynthetic precursor of 20-hydroxyecdysone,</span> which plays an important role in plant defense against arthropods. The LC<sub>50</sub> of the crude extract of <i>V</i>. <i>gardneriana</i> for <i>A</i>. <i>guerreronis</i> was estimated to be 0.185 mg·mL<sup>-1</sup> and LC<sub>80</sub> = 4.123 mg·mL<sup>-1</sup>. Also, the extract was highly repellent to this pest for up to 24 h. The fractions of <i>V</i>. <i>gardneriana</i>, and also squalene, caused mortality to <i>A</i>. <i>guerreronis</i>. The potential of <i>V</i>. <i>gardneriana</i> fractions/squalene as biopesticides for controlling <i>A</i>. <i>guerreronis</i> in coconut plantations </span><span style="font-family:;" "="">is</span><span style="font-family:;" "=""> discussed herein.</span>展开更多
Allelopathy importance and its application under field conditions are reviewed. Recent achievements in the field of plant protection are mainly considered and presented. Natural chemicals of potential use as pesticide...Allelopathy importance and its application under field conditions are reviewed. Recent achievements in the field of plant protection are mainly considered and presented. Natural chemicals of potential use as pesticides, commercialized natural products of pesticidal properties are included. Reported plants of pest control properties, their targeted agricultural pests, formulations, methods of application and activity under field conditions are presented. Allelopathic plant species and implications on their use in the field, positive and negative impacts on cultivated crop species and agricultural pests are discussed. Some research difficulties and experimental methodology problems are mentioned. Prospects of allelopathy as a future strategy for pest management, development of eco-friendly biopesticides and importance for sustainable agriculture and recent developments in allelopathy research are discussed.展开更多
Currently, plant diseases and insect infestations are mainly controlled by the extraneous application of pesticides. Unfortunately, the indiscriminate use of such agrochemicals can cause ecological and environmental p...Currently, plant diseases and insect infestations are mainly controlled by the extraneous application of pesticides. Unfortunately, the indiscriminate use of such agrochemicals can cause ecological and environmental problems, as well as human health hazards. To obviate the potential pollution arising from the application of agrochemicals, biological control of soilborne pathogens or insect pests using antagonistic microorganisms may be employed. Certain soil bacteria, algae, fungi, plants and insects possess the unique ability to produce hydrogen cyanide(HCN), which plays an important role in the biotic interactions of those organisms. In particular, cyanogenic bacteria have been found to inhibit the growth of various pathogenic fungi, weeds, insects, termites and nematodes. Thus, the use of HCN-producing bacteria as biopesticides offers an ecofriendly approach for sustainable agriculture. The enzyme, HCN synthase,involved in the synthesis of HCN, is encoded by the hcnABC gene cluster. The biosynthetic regulation of HCN, antibiotics and fluorescent insecticidal toxins through the conserved global regulatory GacS/GacA system is elaborated in this review, including approaches that may optimize cyanogenesis for enhanced pest control. In addition, the effects of bacterially synthesized HCN on the production of indole acetic acid, antibiotics and fluorescent insecticidal toxins, 1-aminocyclopropane-1-carboxylate deaminase utilization and phosphate solubilization may result in the stimulation of plant growth. A more detailed understanding of HCN biosynthesis and regulation may help to elaborate the precise role of this compound in biotic interactions and sustainable agriculture.展开更多
文摘Biological insecticides have gained importance in modern society because they cause minimal environmental harm and reduce pests’ability to develop resistance.Nomuraea rileyi,a facultative entomopathogenic fungus,is suitable for infecting lepidopteran pests,including Spodoptera litura and Helicoverpa armigera.This work aims to identify a suitable semi-solid fermentation medium to increase the conidia production of N.rileyi and enhance its biological control ability as mycoinsecticide.Research is carried out on broken rice,wheat,sorghum,and corn as substrates,both with and without nutrient addition.It also covers incubation under set conditions,colony harvesting,and conidial yield and germination determination using a haemocytometer.All the study was conducted using a completely randomized design(CRD)and a test of significance was set at p<0.05 using analysis of variance(ANOVA).This work aimed to establish the effect of various substrates on growth and conidia formation in N.rileyi.The highest conidia count of 10×10^(8)CFU/g on day 15.The result was obtained from BR+Nu followed by sorghum 7×10^(8)CFU/g,corn 7×10^(8)CFU/g and wheat 6.5×10^(8)CFU/g.Thus,post-drying,the highest corresponding viability was observed in the product containing BR+Nu–6.20×10^(8)CFU/g.Adding rice bran(BR+RB,150 g each)still increases conidial yield up to 14×10^(8) CFU/g at 15 DAI.These indings thus assert that broken rice and supplementation affect conidia yield and viability.
文摘In this study, toxicity and control efficiency of three nicotine biopesticides against pomegranate aphids were determined via indoor toxicity test and field effica- cy test, aiming at screening the appropriate reagent for prevention and control of pomegranate aphids. The results of indoor toxicity test showed that LC^o of reagent 1, reagent 2 and reagent 3 reached 10 270, 12 810 and 13 040 mg/L, respectively. The results of field efficacy test showed that control efficacy of reagent 1, reagent 2 and reagent 3 reached 60.52%, 34.60% and 46.99%, respectively. Overall, reagent 1 （91% ultrafine powder of tobacco leaves, 3% silicone oil, 2% sasangua cake, 2% emulsifier, 2% ascorbic acid） exhibited the highest control efficiency against pomegranate aphids, which can be further popularized and utilized.
基金financially supported by the Jiangsu Agricultural Scientific Self-innovation Fund,China (CX(15)1057)the Fund of Science and Technology of Jiangsu Province,China (BE2015342)the National Key Research and Development Program of China (2017YFD0200400 and 2016YFD0300706)
文摘The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata lugens.In this study,the selective toxicity of seven commercial biopesticides for C.lividipennis was examined under laboratory conditions; abamectin was the most selective to C.lividipennis,followed by matrine and azadirachtin.Veratridine,rotenone,Bacillus thuringiensis and Beauveria bassiana showed less selectivity for C.lividipennis.Subsequently,matrine,abamectin and azadirachtin were selected for sublethal assessments with respect to C.lividipennis due to their high toxicities to N.lugens.C.lividipennis treated with sublethal concentrations(LC10 and LC20) of the three biopesticides could distinguish volatiles released from healthy and N.lugens-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving miridbugs.Azadirachtin decreased the consumption capability of C.lividipennis when the densities of N.lugens were 20,30,40 and 50 insects per vial.Sublethal concentration treatment did not impact the pre-oviposition period or egg hatchability of C.lividipennis.However,the fecundity of C.lividipennis exposed to azadirachtin and abamectin increased by 27–41% compared to the untreated individuals.In summary,abamectin or matrine together with C.lividipennis could be considered an effective,sustainable pest management strategy for rice.
文摘Biopesticides are derivatives of plants, microorganisms and insects. Substances from plants and animals have been used to manage diseases in crops, animals and humans. Reliance on nature to heal nature is a practise for many people around the world. Use of natural products was overtaken by synthetic chemicals due to their efficacy, reliability and quick knock down effect. However, synthetic pesticides have become a health hazards for humans and environment due to their toxicity and pollution. Biopesticides are potential alternatives to synthetic pesticides. Sources of biopesticides are readily available, easily biodegradable, exhibit various modes of action, are less expensive and have low toxicity to humans and non-target organisms. Neem, pyrethrum, cotton and tobacco are known sources of botanical pesticides and have already been commercialized. Other sources of botanical pesticides include garlic, euphorbia, citrus, pepper among others. Species of Trichoderma, Bacillus, Pseudomonas, Beauveria have been commercialized as microbial pesticides. Biopesticides are however faced with challenges of formulation, registration, commercialization, acceptance and adoption. This paper describes several aspects of biopesticide development, including but not limited to, their sources, production, formulation, commercialization, efficacy and role in sustainable agriculture.
基金Supported by Scientific and Technological Innovation Platform Construction Project for Biological Control Resources Exploration and Utilization in Yunnan Province(202207AB110009)。
文摘[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County.
文摘Neem extract is an alternative insecticide for organic farming and is an allowed component for commercial biopesticide in Korea. However, crop protection properties are not consistent in commercial products. In this report, quantitative analysis of commercial biopesticides for the four insecticidal limonoids of neem extract-azadirachtin A, azadirachtin B, deacetylsalannin and salannin, was conducted through solid phase extraction method with lipophilic-hydrophilic balanced material. The recoveries of the four limonoids ranged from 80.5% to 105%, and their limit of quantitation ranged from 0.028 mg/L to 0.356 mg/L. On the five imported neem extracts, the total contents of the four bioactive limonoids extracted were from 321 mg/L to 5810 mg/L, but there were big variations in the relative composition of the limonoids. The total limonoidal concentrations in 23 commercial bio-pesticides made from neem showed from below LOQ to 7190 mg/L with significant differences in the relative composition. These differences determine the biopesticide’s efficacy on pests, therefore, tracking the active ingredients is necessary for the quality control of commercial bio-pesticides.
文摘Successive cartridge clean-up method for the simultaneous determination of matrine and oxyma- trine in biopesticides containing Sophora flavescens extract was developed and validated by UPLC. The clean-up method was established with ENVI-Carb (0.5 g) and C18 SPE (0.5 g) cartridges for the bioactive alkaloid in biopesticides from S. flavescens, and the eluate was analyzed to quantify the matrine and oxymatrine by UPLC. The developed method was validated, and the recovery and LOQ of both materials were 105.0% and 103.6%, and 0.050 and 0.684 mg·kg-1, respectively. Of the twenty one samples, the total content of matrines were analyzed by using the developed method and the result showed the developed successive clean-up method could contribute to the manufacture and control of biopesticides including matrines, and can be ap- plied to the method development for the analysis of alkaloid materials in biopesticides.
基金the National Natural Science Foundation of China(72103188,72061147002,71974220)the Social Science Foundation of Beijing(21JCC100)+1 种基金the National Social Science Foundation of China(18ZDA074)the 2115 Talent Development Program of China Agricultural University.
文摘Excessive application of mineral fertilizers and synthetic pesticides poses a substantial threat to the soil and water environment and food security.Organic fertilizer and biopesticides have gradually become essential technology for reducing mineral fertilizer and pesticide inputs.In the process,the technical environment is critical for promoting farmer behavior related to the adoption of organic fertilizer and biopesticides.This paper analyzes the influence of the technical environment on farmer behavior related to the adoption of organic fertilizer and biopesticides based on a survey of 1282 farmers in Heilongjiang Province,China,using the bivariate probit model.The results indicate that(1)farmer behavior related to the adoption applying organic fertilizer and biopesticides were positively correlated;(2)the technical environment had a significant positive impact on farmer behavior related to the adoption of organic fertilizer and biopesticides;and(3)the technical environment had a heterogeneous effect across different groups of farmers.This research provides insights useful for promoting organic fertilizer and biopesticides to farmers.It can be helpful to bundle relevant environmental technologies,conduct technology training for farmers and strengthen the construction of rural information networks.
文摘Cowpea, Vigna unguiculata L. Walp, is an important food grain legume in Niger facing production losses due to insect pests. This study aims to determine the efficiency of non-chemical methods for managing these pests. A trial was conducted during the 2020 and 2022 cropping seasons at the INRAN station in the Maradi region. A Fischer experimental design with 6 repetitions was used to compare 4 treatments: synthetic chemical pesticide;the entomopathogenic fungus Beauveria bassiana;aqueous extracts of neem seeds, and control. Observations were carried out every three days. The cowpea pod-sucking bug, pod borer, and thrips were the main insect pests recorded. In terms of effectiveness, the synthetic pesticide was the best treatment. It reduced insect pest densities by 71.35% to 90.40% in 2020 and by 35.11% to 42.13% in 2022. Grain yields varied between treatments. Neem seed extract followed the synthetic pesticide and significantly reduced insect infestations in both years. The synthetic pesticide and neem seed extract resulted in yields 3 to 5 times higher than the control treatment in 2020. By contrast, B. bassiana 115 and neem seed extract produced similar yields in 2022. Therefore, the results of this study showed that B. bassiana 115 and neem seed extract have insecticidal potential and could be used as an ecological alternative for managing cowpea insect pests in the Sahel.
文摘Biopesticides are attracting interest as alternatives to conventional pesticides but without many of the non-target effects, promising a better record of safety and sustainability in pest control practices. In this article we summarize and discuss the current status and future promise of biopesticides, including how biopesticides use may increase the quality and safety of the food supply.
文摘Plants with bioactive properties are greatly useful in preventing and controlling blood-sucking and disease-vector invertebrates, particularly in developing countries and low-income communities. Their application is a promising alternative to synthetic compounds whose use remains a health, environmental, and economic challenge. However, many are still unknown and unvalued, while others are becoming ignored and threatened. The main objective of this ethnobotanical study is to identify and characterize indigenous and locally grown plants against blood-sucking and disease-vector insects. Salient opportunities and challenges of using these plants are documented and discussed. Semi-structured interviews, using a prepared questionnaire, were conducted with 228 informants. The consensus index (CI) was calculated to analyze the reliability of the collected information. The identified 31 anti-insect plant species belong to 20 botanical families, four morphological categories, and six habitat types. They can be categorized as insecticidal plants (42% of the total), insect repellent (42% of the total), and both insecticidal and insect repellent (16% of the total). More than 54% of these are still abundant in the study area, while about 35.5% have become rare and difficultly accessible. Based on the numerical importance of related anti-insect plant species, the seven targeted blood-sucking insects range in the following decreasing order: Jiggers (16 species) > Fire Ants (9 species) > Flies (8 plants) > Mosquitoes (4 species) > Fleas (2 species) > Bedbugs (1 species) > lice (0 species). The three most commonly used plants, with the highest confirmation indices, are Tetradenia riparia (ICs = 0.712), Eucalyptus globulus subsp. maidenii (ICs = 0.302), and Solanum aculeastrum (ICs = 0.288). The antimicrobial role of many locally grown anti-insect plants and the multiple other associated valorization possibilities are ignored by most informants. Domesticating, propagating, protecting, and promoting the sustainable use of these plants would be an appropriate route for their conservation and continued availability.
文摘Aromatic plant distillation produces residues rich in phenolic compounds known to be bioactive. In this context, residues from the hydrodistillation of Mentha piperita L., Cymbopogon citratus Stapf, and Eucalyptus camaldulensis Dehnh were chemically and biologically analyzed. The ethanol percentages (70%, 50%, and 30%) were evaluated by determining antioxidant activity using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging, total phenolic content, and total flavonoid content. Antifungal activity was evaluated “in vitro” against Fusarium oxysporum, Alternaria alternata, Trametes versicolor, and Coniophora puteana using the diffusion method in malt-agar medium. Antitermite activity was also assessed against Reticulitermes flavipes using the most active extract with low ethanol concentrations (50% and 30%). The optimum ethanol concentration to extract phenolic compounds is 70% for TFC and 30% for TPC, and the antioxidant activity was 0.45 ± 0.01 mg/mL (IC50). The most active extracts were the ethanolic ones, particularly for Eucalyptus camaldulensis, presenting 100% inhibition against the mycelial growth of Coniophora puteana and Alternaria alternata at 1 mg/mL and 0.5 mg/mL, respectively. For antitermite activity, EE50 was the most effective, with a 24% termite survival rate and a degraded food supply surface of 28%. These data suggest the potential use of hydrodistillation residues for biopesticide development.
文摘The present research aimed at producing a biopesticide with proper formulation of invert emulsion (water-in-oil type) and effective strain of?Trichoderma harzianum?then, testing it against pathogens of postharvest mold infections(Botrytis cinerea?&?Penicillium expansum) on fresh fruits of apple and pear. The?proper formulation of invert emulsion (IE#3) used for biopesticide production has the following ingredients (100% w/w): soybean oil (28.50%), coconut oil (19.50%), oil-soluble emulsifier: Tween 20 (2.0%), glycerine (4.25%), water-soluble emulsifier:?dehymuls k (0.75%),?sterile distilled water (22.5%) and conidial suspension of the effective strain ofT. harzianum?“TrichoPAL1” in water (22.5%, concentration 1 ×?107?conidia/ml). Testing efficacy of the produced biopesticide has indicated a significant reduction in the disease lesion diameter of mold infections on wounded apple and pear fruits stored at?20℃?± 1℃?compared to the untreated fruits or control (reduction from up to 38.75 to about 7.50 mm, respectively, according to?the type of mold infections and fruit type). Also, the treatment with the produced biopesticide has resulted in?a long protection period from mold infections on wounded and un-wounded fresh fruits of apple and pear stored under controlled and semi-commercial conditions(up to 2.5 months according to the type of mold infections and fruit type). In conclusion, the overall results have demonstrated the effectiveness of produced biopesticide on stored fruits under controlled and semi-commercial conditions therefore,?it is recommended to test this effectiveness on marketed fruits stored under variable conditions before applying it at a large scale.
基金Supported by Circular of the Ministry of Agriculture on Approval of the First Batch of the National Demonstration Zones for Modern Agriculture(NJF[2010]22)Fundamental Research Funds for the Central Universities(XDJK2015C057,SWU114046)~~
文摘In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were conducted to study the control effect of biopesticide chitosan, biochemical compound Prochloraz-Chitosan and chemical pesticides difenoconazole and thifluzamide by field efficacy trials. The results showed that under serious occurrence of anthracnose in autumn greenhouse cowpea, when the 2% chJtosan AS （2 250 g/hm^2）, 46% Prochloraz-Chitosan EW （450 g/hm^2）, 10% difenoconazole WG （900 g/hm^2, CK） or 24% thifluzamide SC （360 ml/hm^2） were applied three times with an interval of 5-8 d, the control efficiency on day 7, 14 and 20 reached 83%, 78% and 73%, respectively. No significant difference was found in control efficiency among the four kinds of agents. These four kinds of agents, especially chitosan and Prochloraz-Chitosan, could be used as ideal agents for controlling anthracnose, as well as for modern agricultural demonstration zones, pollution-free agricultural products, green agricultural products and organic agricultural products,thereby meeting the develop- ment needs of ＇modern agriculture, green agriculture, organic agriculture and ecological agriculture.
文摘The use of chemotherapeutants, antibiotics, and pesticides in aqua farms and non-adherence to scientific management practices have resulted in adverse impact on aquacultural production, serious outbreaks of diseases, development of drug resistance in microbes, and accumulation of antibiotics and pesticide residues in finfish and shellfish and environmental pollution. As a result, a need has been felt by the aquaculturists as well as aquatic animal health management professionals to find a suitable alternative therapy in place of antibiotics and chemotherapy. Phytotherapy has come to be recognized as a handy and viable alternative to chemotherapy, as it is economical, effective, non-resistance forming, renewable, eco-friendly and farmer-friendly. Although the use of medicinal plants is known to humanity since the dawn of human civilization for the treatment and control of human and animal diseases but its importance in combating finfish and shellfish diseases has been realized only recently. The phytotherapy of aquacultural diseases is in its infancy in most part of the world except in China to some extent. However, many important contributions in this field by different workers during the last quarter of the 20th century and early 21st century have shown encouraging results and opened new vistas in phytotherapy of aquatic animals. The present review crtically evaluates the present status of knowledge of phytotherapy in the world combating various aquacultural diseases, identifying the bottlenecks and suggests remedial measures.
文摘Plant extracts have long been used in commercial agriculture as anti-microbial tools in food safety applications. These offer growers and agrobiologists many unique benefits which include their eco-friendliness. This work reviews the situation of Biofungicides reconnaissance in reference to fungal disease of cowpea. Twenty different pathogens were associated with various fungal diseases of cowpea and, only the species of Colletotrichum was found to have the virulence and propensity of afflicting a 100% infection on a single susceptible cowpea crop. Plant families under the affliction of Colletotrichum were analyzed. The different forms of botanicals so far availed for use as potential biofungicidal were identified. Eighteen plant families were found to represent the entire plants and plant materials agrobiologically screened within a range of thirteen years and found to habour large spectra of species containing substances of biofungicidal potentials. Current position in the use of Botanicals to combat agricultural pests and disease is 7% of the total cowpea disease management options.
文摘Essential oils （EOs） from Rosemary, Rosmarinus officinalis （Lamiaceae）, Orange, Citrus sinensis （Rutaceae）, Lavandula, Lavandula angustifolia （Lamiaceae）, False Yellowhead, Dittrichia viscosa （Asteraceae） and their major components were evaluated against root knot nematode, Meloidogyne incognita and Meloidogynejavanica. Second stage juveniles＇ （J2） paralysis and egg hatch inhibition were studied, while pulverized plant parts were tested for nematodes biological cycle arrest. All EOs paralyzed J2 and M. incognita were found more sensitive than M javanica with the EC50/4 days calculated at 250, 3,650 and 4,260 12g/mL for 1）. viscosa, L. angustifolia and R. officinalis, respectively. Similarly, all EOs inhibited nematodes egg hatch and D. viscosa exhibited the highest inhibition on egg hatch （100% inhibition at 5 μg/mL）. A significant influence of constituent terpenes （limonene, 1,8-cineole, linalool, camphor, L-borneol, caryophyllene oxide, β-eudesmol） dose and exposure time was indicated on egg hatch inhibition （56% to 100% at 500 μg/mL and 1,000 μg/mL）, while only β-eudesmol achieved paralysis of J2 and specifically against M. incognita （EC50/1d = 50μg/mL）. Interestingly, the most active botanical species arresting Meloidogyne spp. biological cycle in soil was C. sinensis （EC50 = 2 mg/g） and the most sensitive nematode species was M. javanica. The larvicidal and egg hatch inhibition activity holds promise towards the optimization of artificial terpene mixtures as novel and effective natural nematicides. Complex interactions of primary compounds and subsequent decomposition derivates compose efficacy profile of soil amendments.
文摘The coconut mite, <i>Aceria</i> <i>guerreronis</i> Keifer (Acari: Eriophyidae), is a major pest of coconut plantations (<i>Cocos</i> <i>nucifera</i> L.) worldwide. Here, we conducted a bioguided<span style="font-family:;" "=""> </span><span style="font-family:;" "="">phytochemical approach using toxicity and repellency bioassays of nonpolar extract and its fractions of <i>Vitex</i> <i>gardneriana</i> Schauer (Lamiaceae) leaves to this pest. Nonpolar crude extract was fractionated by column chromatography using solvents with increased polarity and binary mixtures, resulting in five semipurified groups. The biomonitor</span><span style="font-family:;" "="">ing</span><span style="font-family:;" "=""> bioassay provided active fractions and led to the isolation and characterization of the <span>bioactive compound squalene, a biosynthetic precursor of 20-hydroxyecdysone,</span> which plays an important role in plant defense against arthropods. The LC<sub>50</sub> of the crude extract of <i>V</i>. <i>gardneriana</i> for <i>A</i>. <i>guerreronis</i> was estimated to be 0.185 mg·mL<sup>-1</sup> and LC<sub>80</sub> = 4.123 mg·mL<sup>-1</sup>. Also, the extract was highly repellent to this pest for up to 24 h. The fractions of <i>V</i>. <i>gardneriana</i>, and also squalene, caused mortality to <i>A</i>. <i>guerreronis</i>. The potential of <i>V</i>. <i>gardneriana</i> fractions/squalene as biopesticides for controlling <i>A</i>. <i>guerreronis</i> in coconut plantations </span><span style="font-family:;" "="">is</span><span style="font-family:;" "=""> discussed herein.</span>
文摘Allelopathy importance and its application under field conditions are reviewed. Recent achievements in the field of plant protection are mainly considered and presented. Natural chemicals of potential use as pesticides, commercialized natural products of pesticidal properties are included. Reported plants of pest control properties, their targeted agricultural pests, formulations, methods of application and activity under field conditions are presented. Allelopathic plant species and implications on their use in the field, positive and negative impacts on cultivated crop species and agricultural pests are discussed. Some research difficulties and experimental methodology problems are mentioned. Prospects of allelopathy as a future strategy for pest management, development of eco-friendly biopesticides and importance for sustainable agriculture and recent developments in allelopathy research are discussed.
文摘Currently, plant diseases and insect infestations are mainly controlled by the extraneous application of pesticides. Unfortunately, the indiscriminate use of such agrochemicals can cause ecological and environmental problems, as well as human health hazards. To obviate the potential pollution arising from the application of agrochemicals, biological control of soilborne pathogens or insect pests using antagonistic microorganisms may be employed. Certain soil bacteria, algae, fungi, plants and insects possess the unique ability to produce hydrogen cyanide(HCN), which plays an important role in the biotic interactions of those organisms. In particular, cyanogenic bacteria have been found to inhibit the growth of various pathogenic fungi, weeds, insects, termites and nematodes. Thus, the use of HCN-producing bacteria as biopesticides offers an ecofriendly approach for sustainable agriculture. The enzyme, HCN synthase,involved in the synthesis of HCN, is encoded by the hcnABC gene cluster. The biosynthetic regulation of HCN, antibiotics and fluorescent insecticidal toxins through the conserved global regulatory GacS/GacA system is elaborated in this review, including approaches that may optimize cyanogenesis for enhanced pest control. In addition, the effects of bacterially synthesized HCN on the production of indole acetic acid, antibiotics and fluorescent insecticidal toxins, 1-aminocyclopropane-1-carboxylate deaminase utilization and phosphate solubilization may result in the stimulation of plant growth. A more detailed understanding of HCN biosynthesis and regulation may help to elaborate the precise role of this compound in biotic interactions and sustainable agriculture.
