In this study,the yield of exopolysacchrides(LCEPS)produced by Leuconostoc citreum BH10 was optimized throughthe improvement of fermentation conditions.A three-level four-factor central composite design coupled withre...In this study,the yield of exopolysacchrides(LCEPS)produced by Leuconostoc citreum BH10 was optimized throughthe improvement of fermentation conditions.A three-level four-factor central composite design coupled withresponse surface methodology was employed to elucidate the interactions among four design variables,namelyfermentation time(X_(1)),fermentation temperature(X_(2)),sucrose concentration(X_(3)),and inoculation amount(X_(4))over abroad range of process conditions and determine their effects on LCEPS production.Based on the developed models,under the optimum process conditions of 49.99 h,25.59℃,21.66,and 3.00%,the obtained maximum production ofLCEPS was up to 55.96 g/L.Besides,the produced LCEPS presented better thermal stability than the original dextranand rendered finely emulsifying properties compared with commercial emulsifiers.Moreover,the LCEPS possessedgood antioxidant activities and showed certain biofilm inhibition against Staphylococcus aureus ATCC 6538 strain,which indicated that it has fine potential to be used as an excellent additive into the food industry.展开更多
基金supported by the Fundamental Research Funds for the Central Universities(No.KYZZ2022001)Priority Academic Program Development of Jiangsu Higher Education Institutions(080-820830)Jiangsu Agricultural Science and Technology Innovation Fund(Grant No.CX(21)2003).
文摘In this study,the yield of exopolysacchrides(LCEPS)produced by Leuconostoc citreum BH10 was optimized throughthe improvement of fermentation conditions.A three-level four-factor central composite design coupled withresponse surface methodology was employed to elucidate the interactions among four design variables,namelyfermentation time(X_(1)),fermentation temperature(X_(2)),sucrose concentration(X_(3)),and inoculation amount(X_(4))over abroad range of process conditions and determine their effects on LCEPS production.Based on the developed models,under the optimum process conditions of 49.99 h,25.59℃,21.66,and 3.00%,the obtained maximum production ofLCEPS was up to 55.96 g/L.Besides,the produced LCEPS presented better thermal stability than the original dextranand rendered finely emulsifying properties compared with commercial emulsifiers.Moreover,the LCEPS possessedgood antioxidant activities and showed certain biofilm inhibition against Staphylococcus aureus ATCC 6538 strain,which indicated that it has fine potential to be used as an excellent additive into the food industry.
