Bet v 1基因家族编码植物病程相关蛋白PR10,参与植物的非生物和生物胁迫应答。本研究对新疆沙冬青Bet v 1基因家族进行筛选和进化分析,并通过实时荧光定量PCR分析了Bet v 1基因在低温和渗透胁迫下的表达模式。结果表明筛选到27个新疆沙...Bet v 1基因家族编码植物病程相关蛋白PR10,参与植物的非生物和生物胁迫应答。本研究对新疆沙冬青Bet v 1基因家族进行筛选和进化分析,并通过实时荧光定量PCR分析了Bet v 1基因在低温和渗透胁迫下的表达模式。结果表明筛选到27个新疆沙冬青Bet v 1基因家族成员,均包含内含子,分布于6条染色体上。在新疆沙冬青中有11个Bet v 1基因在大豆、拟南芥基因组中具有直系同源基因。该基因家族成员的启动子中含有大量与逆境胁迫和激素响应相关的元件。基因表达分析显示EVM0015604.1、EVM0020624.1、EVM000-0761.1、EVM0033568.1和EVM0004485.1的表达量在低温和渗透胁迫下升高,推测Bet v 1基因参与了新疆沙冬青响应低温和干旱胁迫。本研究为理解植物Bet v 1基因的功能提供了一定的理论依据。展开更多
In light of the growing urgency to address environmental degradation and improve carbon sequestration strategies,this study rigorously investigates the potential of Cistus ladaniferus as a viable feedstock for biochar...In light of the growing urgency to address environmental degradation and improve carbon sequestration strategies,this study rigorously investigates the potential of Cistus ladaniferus as a viable feedstock for biochar and activated carbon production.The influence of pyrolysis temperature,heating rate and particle size on biochar yield was systematically examined.The results demonstrate that increasing pyrolysis temperature and heating rate significantly reduces biochar yield,while particle size plays a crucial role in thermal degradation and biochar retention.To evaluate the structural and chemical properties of the materials,various characterization techniques were employed,including Fourier-transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),and energy-dispersive X-ray analysis(EDXA).FTIR identified key functional groups,while SEM and EDXA provided valuable insights into the morphology and elemental composition of the materials.Activated carbons exhibited enhanced porosity and carbon content compared to their biochar counterparts,achieving specific surface areas of up to 1210 m^(2) g^(-1) for acidactivated shells(AC-Sha).The Brunauer-Emmett-Teller(BET)method confirmed the mesoporous characteristics of these materials,with AC-Sa displaying a surface area of 678.74 m^(2) g^(-1) and an average pore size of 2.73 nm.Elemental analysis revealed that activated carbons possessed a higher carbon content(96.40 wt.%for AC-Sha)and lower oxygen content(2.37 wt.%),highlighting their suitability for applications in adsorption and catalysis.These findings underscore the significant impact of activation processes on the stability and adsorption capabilities of Cistus-derived biochars and activated carbons,paving the way for future research and practical applications in pollution control,carbon sequestration,and bioenergy.展开更多
Saline soils are significantly affected by water-salt phase changes,evaporation,and groundwater during seasonal freezing and thawing.For the study of physical and mechanical properties of saline soils,solubility is an...Saline soils are significantly affected by water-salt phase changes,evaporation,and groundwater during seasonal freezing and thawing.For the study of physical and mechanical properties of saline soils,solubility is an important indicator that varies with temperature.However,there have been very limited computational studies on solubility at low temperatures.The model for calculating the solubility of Na_(2)SO_(4)-NaCl-H_(2)O ternary system under low temperature conditions was constructed in this paper,based on the Pitzer and BET models.Improvements were made to the parametersФandγin the Pitzer model,while improvements were made to the parameters c,r,and aw in the BET model.The solubility changes within the range of 273.15 K–373.15 K were calculated and validated by combining them with indoor experiments.It was found that both the improved Pitzer model and BET model accurately predicted relative equilibrium solubility data of the Na_(2)SO_(4)-NaCl-H_(2)O ternary system at temperatures ranging from 273.15 K to 373.15 K.Additionally,compared with the Pitzer model,the BET model had advantages such as easy parameter acquisition and wider application range.The findings from this research hold great significance for understanding the process and patterns of salt analysis during soil freeze-thaw cycles as well as providing a scientific foundation for further comprehension of phase change laws and physical properties related to saline soils.展开更多
The enrichment of low-grade phosphate rock is an important process to realize sustainable support of phosphorus resources. An aqueous solution containing Ca(NO_(3))_(2) and Mg(NO_(3))_(2) is produced during the enrich...The enrichment of low-grade phosphate rock is an important process to realize sustainable support of phosphorus resources. An aqueous solution containing Ca(NO_(3))_(2) and Mg(NO_(3))_(2) is produced during the enrichment of low-grade phosphate rock by leaching of HNO_(3) or calcination coupling with leaching of NH_(4)NO_(3) solution. Preparation liquid fertilizer is a preferential way to utilize it. The liquid−solid phase diagrams of Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O, KNO_(3)-Mg(NO_(3))_(2)-H_(2)O, KNO_(3)-Ca(NO_(3))_(2)-H_(2)O and KNO_(3)-Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O systems at 313.15 K were studied by isothermal dissolution equilibrium method. Two crystallization regions of Ca(NO_(3))_(2)·4H_(2)O and Mg(NO_(3))_(2)·6H_(2)O were observed in the phase diagram of the ternary system Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O, a liquid fertilizer with a maximal total nutrient content of 27.46% and a nutrients ratio of N:Ca:Mg = 8.40:10.37:1 can be formed. A homogenous solution can be formed by mixing Ca(NO_(3))_(2)·4H_(2)O and Mg(NO_(3))_(2)·6H_(2)O. In the ternary system KNO_(3)-Mg(NO_(3))_(2)-H_(2)O, the crystallization regions of KNO_(3), Mg(NO_(3))_(2)·6H_(2)O and the co-crystallization region of KNO_(3) and Mg(NO_(3))_(2)·6H_(2)O were observed. The obtained maximal total nutrient content of liquid fertilizer is 23.32% with the ratio of N:K_(2)O = 1:3.39. In the ternary system KNO_(3)-Ca(NO_(3))_(2)-H_(2)O, the crystallization regions of Ca(NO_(3))_(2)·4H_(2)O and KNO_(3) were observed. The obtained maximal total nutrient content of liquid fertilizer is 38.41% with the ratio of N:K_(2)O:Ca = 1.05:1.18:1. A homogenous solution can also be formed by mixing Ca(NO_(3))_(2)·4H_(2)O and KNO_(3) directly. In the quaternary system KNO_(3)-Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O, the crystallization regions of Ca(NO_(3))_(2)·4H_(2)O, Mg(NO_(3))_(2)·6H_(2)O and KNO_(3) and the co-crystallization region of KNO_(3) and Mg(NO_(3))_(2)·6H_(2)O were observed. The obtained maximal total nutrient content of liquid fertilizer is 38.41% with the ratio of N:K_(2)O:Ca = 1.05:1.18:1. The modified BET model was successfully used to fit the solubility curves. The results can provide a guidance for the formulation of water-soluble fertilizers of N-(K, Ca, Mg).展开更多
旨在探究抑制水牛BET(bromodomain and extra terminal,BET)蛋白对支持细胞自噬调控的影响。以水牛支持细胞(sertoli cells,SCs)为研究对象,首先从睾丸中分离纯化得到高纯度的SCs,通过免疫荧光法及RT-PCR法检测出水牛SCs的标记基因均正...旨在探究抑制水牛BET(bromodomain and extra terminal,BET)蛋白对支持细胞自噬调控的影响。以水牛支持细胞(sertoli cells,SCs)为研究对象,首先从睾丸中分离纯化得到高纯度的SCs,通过免疫荧光法及RT-PCR法检测出水牛SCs的标记基因均正常表达。结果显示,BET蛋白被抑制后,水牛SCs形态和数量发生显著变化,细胞的体积变大,数量减少,折光性减弱,免疫荧光分析和MDC分析也证实其生物学功能受到影响。超微结构分析发现,水牛SCs中产生大量自噬体、自噬溶酶体以及自噬空泡,且自噬相关标记基因的表达量显著提升。说明抑制BET蛋白可引起水牛SCs活性和自噬活性降低,并导致自噬体增多。结果表明,本试验为BET蛋白调控雄性生殖机制的研究提供了依据。展开更多
1文献来源SHAPIRO G I,LoRUSSO P,DOWLATI A,et al.A phase 1 study of RO6870810,a novel bromodomain and extra-terminal protein inhibitor,in patients with NUT carcinoma,other solid tumours,or diffuse large B-cell lymphoma[...1文献来源SHAPIRO G I,LoRUSSO P,DOWLATI A,et al.A phase 1 study of RO6870810,a novel bromodomain and extra-terminal protein inhibitor,in patients with NUT carcinoma,other solid tumours,or diffuse large B-cell lymphoma[J].Br J Cancer,2021,124(4):744−753.doi:10.1038/s41416-020-01180-1.2研究背景N端赖氨酸残基的组蛋白乙酰化是一种常见的翻译后修饰,在转录调控中起重要作用。被称为溴结构域(bromodomains,BRD)的蛋白质相互作用结构域能够识别并结合赖氨酸乙酰化基序,从而允许含BRD的蛋白质影响转录。BRD和超末端结构域(BRD and extra-terminal,BET)蛋白通过BRD模块中的疏水口袋与乙酰化染色质结合,随后募集并稳定调节转录效应因子。展开更多
1文献来源AMERATUNGA M,BRANA I,BONO P,et al.First-in-human phase 1 open label study of the BET inhibitor ODM-207 in patients with selected solid tumours[J].Br J Cancer,2020,123(12):1730−1736.doi:10.1038/s41416-020-0107...1文献来源AMERATUNGA M,BRANA I,BONO P,et al.First-in-human phase 1 open label study of the BET inhibitor ODM-207 in patients with selected solid tumours[J].Br J Cancer,2020,123(12):1730−1736.doi:10.1038/s41416-020-01077-z.2证据水平2a。3研究背景溴结构域和超末端结构域(bromodomain and extra-terminal,BET)家族(BRD2、BRD3、BRD4和BRDT)包含两个串联的异染色质蛋白域,这些域可结合乙酰化赖氨酸残基,促进转录蛋白招募到染色质上。BRD4定位于染色质的启动子和增强子区域,并通过与中介复合体和pTEFb的相互作用来调节RNA聚合酶Ⅱ介导的延伸和转录。展开更多
Dear Editor,Ferroptosis,an iron-dependent form of cell death driven by overwhelming lipid peroxidation,represents a vulnerability in cancers,and therapeutic strategies to further potentiate ferroptosis hold great pote...Dear Editor,Ferroptosis,an iron-dependent form of cell death driven by overwhelming lipid peroxidation,represents a vulnerability in cancers,and therapeutic strategies to further potentiate ferroptosis hold great potential for melanoma treatment.展开更多
1文献来源PIHA-PAUL S A,HANN C L,FRENCH C A,et al.Phase 1 study of molibresib(GSK525762),a bromodomain and extra-terminal domain protein inhibitor,in NUT carcinoma and other solid tumors[J].JNCI Cancer Spectr,2019,4(2)...1文献来源PIHA-PAUL S A,HANN C L,FRENCH C A,et al.Phase 1 study of molibresib(GSK525762),a bromodomain and extra-terminal domain protein inhibitor,in NUT carcinoma and other solid tumors[J].JNCI Cancer Spectr,2019,4(2):pkz093.doi:10.1093/jncics/pkz093.2研究背景溴结构域和超末端结构域(bromodomain and extraterminal domain,BET)家族是表观基因组的调节因子,也是肿瘤细胞生存所依赖的肿瘤相关基因表达的关键驱动因子,一些重要的原癌基因如MYC、BCL2、CDK6均受到BET蛋白的调控,BET蛋白的异常激活与包括癌症在内的各种人类疾病密切相关,包括罕见且具有高度侵袭性的NUT癌。展开更多
文摘Bet v 1基因家族编码植物病程相关蛋白PR10,参与植物的非生物和生物胁迫应答。本研究对新疆沙冬青Bet v 1基因家族进行筛选和进化分析,并通过实时荧光定量PCR分析了Bet v 1基因在低温和渗透胁迫下的表达模式。结果表明筛选到27个新疆沙冬青Bet v 1基因家族成员,均包含内含子,分布于6条染色体上。在新疆沙冬青中有11个Bet v 1基因在大豆、拟南芥基因组中具有直系同源基因。该基因家族成员的启动子中含有大量与逆境胁迫和激素响应相关的元件。基因表达分析显示EVM0015604.1、EVM0020624.1、EVM000-0761.1、EVM0033568.1和EVM0004485.1的表达量在低温和渗透胁迫下升高,推测Bet v 1基因参与了新疆沙冬青响应低温和干旱胁迫。本研究为理解植物Bet v 1基因的功能提供了一定的理论依据。
文摘In light of the growing urgency to address environmental degradation and improve carbon sequestration strategies,this study rigorously investigates the potential of Cistus ladaniferus as a viable feedstock for biochar and activated carbon production.The influence of pyrolysis temperature,heating rate and particle size on biochar yield was systematically examined.The results demonstrate that increasing pyrolysis temperature and heating rate significantly reduces biochar yield,while particle size plays a crucial role in thermal degradation and biochar retention.To evaluate the structural and chemical properties of the materials,various characterization techniques were employed,including Fourier-transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),and energy-dispersive X-ray analysis(EDXA).FTIR identified key functional groups,while SEM and EDXA provided valuable insights into the morphology and elemental composition of the materials.Activated carbons exhibited enhanced porosity and carbon content compared to their biochar counterparts,achieving specific surface areas of up to 1210 m^(2) g^(-1) for acidactivated shells(AC-Sha).The Brunauer-Emmett-Teller(BET)method confirmed the mesoporous characteristics of these materials,with AC-Sa displaying a surface area of 678.74 m^(2) g^(-1) and an average pore size of 2.73 nm.Elemental analysis revealed that activated carbons possessed a higher carbon content(96.40 wt.%for AC-Sha)and lower oxygen content(2.37 wt.%),highlighting their suitability for applications in adsorption and catalysis.These findings underscore the significant impact of activation processes on the stability and adsorption capabilities of Cistus-derived biochars and activated carbons,paving the way for future research and practical applications in pollution control,carbon sequestration,and bioenergy.
基金supported by the National key research&development program of China(Grant no.2021YFD1900600)the National Natural Science Foundation of Ningxia(2023AAC05014)+1 种基金the Ningxia Key Research and Development Program(Special Talents)(2023BSB03021)the Scientific Research Program of University of Ningxia Education Department(NYG2024049)。
文摘Saline soils are significantly affected by water-salt phase changes,evaporation,and groundwater during seasonal freezing and thawing.For the study of physical and mechanical properties of saline soils,solubility is an important indicator that varies with temperature.However,there have been very limited computational studies on solubility at low temperatures.The model for calculating the solubility of Na_(2)SO_(4)-NaCl-H_(2)O ternary system under low temperature conditions was constructed in this paper,based on the Pitzer and BET models.Improvements were made to the parametersФandγin the Pitzer model,while improvements were made to the parameters c,r,and aw in the BET model.The solubility changes within the range of 273.15 K–373.15 K were calculated and validated by combining them with indoor experiments.It was found that both the improved Pitzer model and BET model accurately predicted relative equilibrium solubility data of the Na_(2)SO_(4)-NaCl-H_(2)O ternary system at temperatures ranging from 273.15 K to 373.15 K.Additionally,compared with the Pitzer model,the BET model had advantages such as easy parameter acquisition and wider application range.The findings from this research hold great significance for understanding the process and patterns of salt analysis during soil freeze-thaw cycles as well as providing a scientific foundation for further comprehension of phase change laws and physical properties related to saline soils.
基金support from the National Key Research and Development Program of China(2022YFC2904704)the Fundamental Research Funds for the Central Universities(SCU2024D009)。
文摘The enrichment of low-grade phosphate rock is an important process to realize sustainable support of phosphorus resources. An aqueous solution containing Ca(NO_(3))_(2) and Mg(NO_(3))_(2) is produced during the enrichment of low-grade phosphate rock by leaching of HNO_(3) or calcination coupling with leaching of NH_(4)NO_(3) solution. Preparation liquid fertilizer is a preferential way to utilize it. The liquid−solid phase diagrams of Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O, KNO_(3)-Mg(NO_(3))_(2)-H_(2)O, KNO_(3)-Ca(NO_(3))_(2)-H_(2)O and KNO_(3)-Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O systems at 313.15 K were studied by isothermal dissolution equilibrium method. Two crystallization regions of Ca(NO_(3))_(2)·4H_(2)O and Mg(NO_(3))_(2)·6H_(2)O were observed in the phase diagram of the ternary system Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O, a liquid fertilizer with a maximal total nutrient content of 27.46% and a nutrients ratio of N:Ca:Mg = 8.40:10.37:1 can be formed. A homogenous solution can be formed by mixing Ca(NO_(3))_(2)·4H_(2)O and Mg(NO_(3))_(2)·6H_(2)O. In the ternary system KNO_(3)-Mg(NO_(3))_(2)-H_(2)O, the crystallization regions of KNO_(3), Mg(NO_(3))_(2)·6H_(2)O and the co-crystallization region of KNO_(3) and Mg(NO_(3))_(2)·6H_(2)O were observed. The obtained maximal total nutrient content of liquid fertilizer is 23.32% with the ratio of N:K_(2)O = 1:3.39. In the ternary system KNO_(3)-Ca(NO_(3))_(2)-H_(2)O, the crystallization regions of Ca(NO_(3))_(2)·4H_(2)O and KNO_(3) were observed. The obtained maximal total nutrient content of liquid fertilizer is 38.41% with the ratio of N:K_(2)O:Ca = 1.05:1.18:1. A homogenous solution can also be formed by mixing Ca(NO_(3))_(2)·4H_(2)O and KNO_(3) directly. In the quaternary system KNO_(3)-Ca(NO_(3))_(2)-Mg(NO_(3))_(2)-H_(2)O, the crystallization regions of Ca(NO_(3))_(2)·4H_(2)O, Mg(NO_(3))_(2)·6H_(2)O and KNO_(3) and the co-crystallization region of KNO_(3) and Mg(NO_(3))_(2)·6H_(2)O were observed. The obtained maximal total nutrient content of liquid fertilizer is 38.41% with the ratio of N:K_(2)O:Ca = 1.05:1.18:1. The modified BET model was successfully used to fit the solubility curves. The results can provide a guidance for the formulation of water-soluble fertilizers of N-(K, Ca, Mg).
文摘旨在探究抑制水牛BET(bromodomain and extra terminal,BET)蛋白对支持细胞自噬调控的影响。以水牛支持细胞(sertoli cells,SCs)为研究对象,首先从睾丸中分离纯化得到高纯度的SCs,通过免疫荧光法及RT-PCR法检测出水牛SCs的标记基因均正常表达。结果显示,BET蛋白被抑制后,水牛SCs形态和数量发生显著变化,细胞的体积变大,数量减少,折光性减弱,免疫荧光分析和MDC分析也证实其生物学功能受到影响。超微结构分析发现,水牛SCs中产生大量自噬体、自噬溶酶体以及自噬空泡,且自噬相关标记基因的表达量显著提升。说明抑制BET蛋白可引起水牛SCs活性和自噬活性降低,并导致自噬体增多。结果表明,本试验为BET蛋白调控雄性生殖机制的研究提供了依据。
文摘1文献来源SHAPIRO G I,LoRUSSO P,DOWLATI A,et al.A phase 1 study of RO6870810,a novel bromodomain and extra-terminal protein inhibitor,in patients with NUT carcinoma,other solid tumours,or diffuse large B-cell lymphoma[J].Br J Cancer,2021,124(4):744−753.doi:10.1038/s41416-020-01180-1.2研究背景N端赖氨酸残基的组蛋白乙酰化是一种常见的翻译后修饰,在转录调控中起重要作用。被称为溴结构域(bromodomains,BRD)的蛋白质相互作用结构域能够识别并结合赖氨酸乙酰化基序,从而允许含BRD的蛋白质影响转录。BRD和超末端结构域(BRD and extra-terminal,BET)蛋白通过BRD模块中的疏水口袋与乙酰化染色质结合,随后募集并稳定调节转录效应因子。
文摘1文献来源AMERATUNGA M,BRANA I,BONO P,et al.First-in-human phase 1 open label study of the BET inhibitor ODM-207 in patients with selected solid tumours[J].Br J Cancer,2020,123(12):1730−1736.doi:10.1038/s41416-020-01077-z.2证据水平2a。3研究背景溴结构域和超末端结构域(bromodomain and extra-terminal,BET)家族(BRD2、BRD3、BRD4和BRDT)包含两个串联的异染色质蛋白域,这些域可结合乙酰化赖氨酸残基,促进转录蛋白招募到染色质上。BRD4定位于染色质的启动子和增强子区域,并通过与中介复合体和pTEFb的相互作用来调节RNA聚合酶Ⅱ介导的延伸和转录。
基金This work was supported by grants from the National Natural Science Foundation of China(82103183,82102803,82272849)the Natural Science Foundation of Hunan Province(2022JJ40767,2021JJ40976)+1 种基金the Natural Science Fund for Outstanding Youths in Hunan Province(2023JJ20093)the National Key Research and Development Program(2022YFC2504700).
文摘Dear Editor,Ferroptosis,an iron-dependent form of cell death driven by overwhelming lipid peroxidation,represents a vulnerability in cancers,and therapeutic strategies to further potentiate ferroptosis hold great potential for melanoma treatment.
文摘1文献来源PIHA-PAUL S A,HANN C L,FRENCH C A,et al.Phase 1 study of molibresib(GSK525762),a bromodomain and extra-terminal domain protein inhibitor,in NUT carcinoma and other solid tumors[J].JNCI Cancer Spectr,2019,4(2):pkz093.doi:10.1093/jncics/pkz093.2研究背景溴结构域和超末端结构域(bromodomain and extraterminal domain,BET)家族是表观基因组的调节因子,也是肿瘤细胞生存所依赖的肿瘤相关基因表达的关键驱动因子,一些重要的原癌基因如MYC、BCL2、CDK6均受到BET蛋白的调控,BET蛋白的异常激活与包括癌症在内的各种人类疾病密切相关,包括罕见且具有高度侵袭性的NUT癌。
