Low-cost and biodegradable photothermal wound dressings with remarkable therapeutic effects are highly desirable for next-generation wound healing.Herein,we report an efficient photothermal wound dressing mat made of ...Low-cost and biodegradable photothermal wound dressings with remarkable therapeutic effects are highly desirable for next-generation wound healing.Herein,we report an efficient photothermal wound dressing mat made of tellurium nanosheet(TeNS)-loaded electrospun polycaprolactone/gelatin(PCL/GEL)nanofibers.The TeNS-loaded PCL/GEL nanofibrous architectures showed antibacterial efficacy against Escherichia coli and Staphylococcus aureus of 87.68%and 94.57%,respectively.Under near-infrared+(NIR)light illumination,they can facilitate cell proliferation as revealed by in vitro scratch assay.The results from in vivo skin wounds combined with tissue staining experiments further showed that the TeNS-loaded dressing could substantially promote wound healing under photothermal conditions.Using immunohistochemical analysis,we found that the TeNS-loaded PCL/GEL nanofibers NIR group have a high expression of specific antigens in epidermal growth factor(EGF)(P<0.001)and endothelial cell adhesion molecule-31(CD31,P<0.05),verifying that the nanofibrous mat can stimulate EGF generation and microvessel proliferation.Furthermore,the PCL/GEL/TeNIR group has the lowest expression in endothelial cell adhesion molecule-68(CD68,P<0.001),suggesting that the nanofibrous mats have a high anti-inflammatory efficiency.Our work sheds light on the development of novel nonanti-inflammatory wound dressings via photothermal sterilization and the promotion of cell growth using two-dimensional(2D)nanosheets.展开更多
基金supported by the Foundation of Health Care for Cadres of Sichuan Provincial Health Commission(Grant No.2023-224).
文摘Low-cost and biodegradable photothermal wound dressings with remarkable therapeutic effects are highly desirable for next-generation wound healing.Herein,we report an efficient photothermal wound dressing mat made of tellurium nanosheet(TeNS)-loaded electrospun polycaprolactone/gelatin(PCL/GEL)nanofibers.The TeNS-loaded PCL/GEL nanofibrous architectures showed antibacterial efficacy against Escherichia coli and Staphylococcus aureus of 87.68%and 94.57%,respectively.Under near-infrared+(NIR)light illumination,they can facilitate cell proliferation as revealed by in vitro scratch assay.The results from in vivo skin wounds combined with tissue staining experiments further showed that the TeNS-loaded dressing could substantially promote wound healing under photothermal conditions.Using immunohistochemical analysis,we found that the TeNS-loaded PCL/GEL nanofibers NIR group have a high expression of specific antigens in epidermal growth factor(EGF)(P<0.001)and endothelial cell adhesion molecule-31(CD31,P<0.05),verifying that the nanofibrous mat can stimulate EGF generation and microvessel proliferation.Furthermore,the PCL/GEL/TeNIR group has the lowest expression in endothelial cell adhesion molecule-68(CD68,P<0.001),suggesting that the nanofibrous mats have a high anti-inflammatory efficiency.Our work sheds light on the development of novel nonanti-inflammatory wound dressings via photothermal sterilization and the promotion of cell growth using two-dimensional(2D)nanosheets.
