A new complex of Ce2L·6H2O(here L=2,6-bis[bis(carboxylatomethyl)amino]methylg-4-chlorophenolate and abbreviated as Cl-HXTA) was synthezied and characterized by IR and EA.Kinetic study on the hydrolysis of the pho...A new complex of Ce2L·6H2O(here L=2,6-bis[bis(carboxylatomethyl)amino]methylg-4-chlorophenolate and abbreviated as Cl-HXTA) was synthezied and characterized by IR and EA.Kinetic study on the hydrolysis of the phosphordiester model compound,BDNPP promoted by the Ce2(III)-L complex was investigated by UV-Vis methods at 25,37℃,and pH 7.20.The result showed that the Ce(Ⅲ) complex could hydrolyze the phosphordiester effectively,with the rate constants as k=626.7 and 821.3(mol/L)-1 min-1,and half lives as 127.65 and 97.41min,reapectively under 25 and 37℃.The investigation on the interaction between the complex and CT DNA indicated that the absorbance of CT DNA was increased and the maximum peak(λmax=260 nm) blue-shifted,while the intensity of fluorescence spectra of EB-DNA was gradually weakened.展开更多
A series of metal complexes were designed and synthesized and a novel binuclear magnesium complex has been selected, namely [Mg-2(dien)Cl(OH2)(2)]Cl-2 . H2O (dien=diethylene-triamine), which can cleave the plasmid pBR...A series of metal complexes were designed and synthesized and a novel binuclear magnesium complex has been selected, namely [Mg-2(dien)Cl(OH2)(2)]Cl-2 . H2O (dien=diethylene-triamine), which can cleave the plasmid pBR322 DNA effectively in close to physiological condition without adding any external materials. Through biological and chemical methods, especially the comparative experiments, we find the interaction between the complex and DNA belongs to hydrolytic mechanism.展开更多
文摘A new complex of Ce2L·6H2O(here L=2,6-bis[bis(carboxylatomethyl)amino]methylg-4-chlorophenolate and abbreviated as Cl-HXTA) was synthezied and characterized by IR and EA.Kinetic study on the hydrolysis of the phosphordiester model compound,BDNPP promoted by the Ce2(III)-L complex was investigated by UV-Vis methods at 25,37℃,and pH 7.20.The result showed that the Ce(Ⅲ) complex could hydrolyze the phosphordiester effectively,with the rate constants as k=626.7 and 821.3(mol/L)-1 min-1,and half lives as 127.65 and 97.41min,reapectively under 25 and 37℃.The investigation on the interaction between the complex and CT DNA indicated that the absorbance of CT DNA was increased and the maximum peak(λmax=260 nm) blue-shifted,while the intensity of fluorescence spectra of EB-DNA was gradually weakened.
文摘A series of metal complexes were designed and synthesized and a novel binuclear magnesium complex has been selected, namely [Mg-2(dien)Cl(OH2)(2)]Cl-2 . H2O (dien=diethylene-triamine), which can cleave the plasmid pBR322 DNA effectively in close to physiological condition without adding any external materials. Through biological and chemical methods, especially the comparative experiments, we find the interaction between the complex and DNA belongs to hydrolytic mechanism.
