Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport deriv...Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport derived from the green pear‘Zaosu’.A new genetic map was constructed from a population derived from the cross of‘Yuluxiang’and‘Hongzaosu’,from which PpBBX24 was identified as a crucial gene controlling anthocyanin accumulation.Genetic and phylogenetic evidences revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear.A 14 bp deletion was detected in the third exon of PpBBX24 in‘Hongzaosu’,resulting in premature termination of protein translation.The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of PpCHS and PpUFGT.Three independent variations in the BBX24 coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins.The genome of‘Hongzaosu’was assembled using both long-read and short-read sequences.By combining genomic and transcriptomic data,allele-specific expression of PpMYB110a was observed in the fruit skin of‘Hongzaosu',which was likely caused by a large variation in the promoter.This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on‘Hongzaosu’pear.展开更多
Temperature is a crucial environmental cue that governs plant growth and adaptation in natural habitats.PHYTOCHROME INTERACTING FACTOR 4(PIF4)functions as a central regulator promoting thermomor-phogenesis in Arabidop...Temperature is a crucial environmental cue that governs plant growth and adaptation in natural habitats.PHYTOCHROME INTERACTING FACTOR 4(PIF4)functions as a central regulator promoting thermomor-phogenesis in Arabidopsis.An understanding of its precise regulation is essential for optimal thermomor-phogenic growth.Here,we identified two BBX proteins,BBX24 and BBX25,as novel components of the PIF4-mediated thermosensory pathway that promote growth at elevated temperatures.Single and double mutants of bbx24 and bbx25 exhibit moderate to strong temperature-insensitive hypocotyl and cotyledon growth.Elevated temperatures induce BBX24 and BBX25 mRNA expression and protein accumulation.Ge-netic and biochemical analyses reveal that BBX24 and BBX25 enhance PIF4-mediated thermosensory growth by counteracting ELF3,a key component of the evening complex.Whereas ELF3 represses BBX24 and BBX25 gene expression at low ambient temperatures during the evening,the inhibition of ELF3 activity under elevated temperatures increases BBX24 and BBX25 activity.Moreover,BBX24 and BBX25 suppress ELF3 function through direct physical interaction,likely relieving its repression of PIF4 and enhancing thermomorphogenesis.These findings identify the ELF3–BBX24/BBX25–PIF4 axis as a key regulatory module controlling plant growth and development in response to temperature cues.展开更多
CONSTANS(CO)and CONSTANS-LIKE(COL)transcription factors are known to regulate a series of cellular processes,including the transition from vegetative growth to flower development in plants.However,their role in regula...CONSTANS(CO)and CONSTANS-LIKE(COL)transcription factors are known to regulate a series of cellular processes,including the transition from vegetative growth to flower development in plants.However,their role in regulating the fruit chlorophyll content is poorly understood.In this study,SlCOL1,the tomato(Solanum lycopersicum)ortholog of Arabidopsis CONSTANS,was shown to play key roles in controlling fruit chlorophyll.The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit,while the overexpression of SlCOL1 increased it.An analysis of protein-protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE(GLK2),which promotes the stability of its protein.The overexpression of SlCOL1in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit,which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content.These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LR22C150001 to SB,LY22C150003 to JN)the Specialized Research Fund for Major Science and Technique of Zhejiang Province of China(2021C02066-5)+1 种基金the National Natural Science Foundation of China(32072545 to YT,32272639 to SB)the China Agriculture Research System of MOF and MARA(CARS-28)and the Yunnan Science and Technology Talent and Platform Program(202205AF150041)。
文摘Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport derived from the green pear‘Zaosu’.A new genetic map was constructed from a population derived from the cross of‘Yuluxiang’and‘Hongzaosu’,from which PpBBX24 was identified as a crucial gene controlling anthocyanin accumulation.Genetic and phylogenetic evidences revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear.A 14 bp deletion was detected in the third exon of PpBBX24 in‘Hongzaosu’,resulting in premature termination of protein translation.The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of PpCHS and PpUFGT.Three independent variations in the BBX24 coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins.The genome of‘Hongzaosu’was assembled using both long-read and short-read sequences.By combining genomic and transcriptomic data,allele-specific expression of PpMYB110a was observed in the fruit skin of‘Hongzaosu',which was likely caused by a large variation in the promoter.This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on‘Hongzaosu’pear.
基金supported by grants from the Department of Biotechnol-ogy(Ramalingaswami Fellowship Grant,BT/RLF/Re-entry/28/2017)Sci-ence and Engineering Research Board(Start-up Research Grant,SRG/2019/000446)+1 种基金Anusandhan National Research Foundation(Core Research Grant,CRG/2023/001553)Intramural grant(IISER Kolkata),Ministry of Education(MoE/STARS-1/416),Government of India.
文摘Temperature is a crucial environmental cue that governs plant growth and adaptation in natural habitats.PHYTOCHROME INTERACTING FACTOR 4(PIF4)functions as a central regulator promoting thermomor-phogenesis in Arabidopsis.An understanding of its precise regulation is essential for optimal thermomor-phogenic growth.Here,we identified two BBX proteins,BBX24 and BBX25,as novel components of the PIF4-mediated thermosensory pathway that promote growth at elevated temperatures.Single and double mutants of bbx24 and bbx25 exhibit moderate to strong temperature-insensitive hypocotyl and cotyledon growth.Elevated temperatures induce BBX24 and BBX25 mRNA expression and protein accumulation.Ge-netic and biochemical analyses reveal that BBX24 and BBX25 enhance PIF4-mediated thermosensory growth by counteracting ELF3,a key component of the evening complex.Whereas ELF3 represses BBX24 and BBX25 gene expression at low ambient temperatures during the evening,the inhibition of ELF3 activity under elevated temperatures increases BBX24 and BBX25 activity.Moreover,BBX24 and BBX25 suppress ELF3 function through direct physical interaction,likely relieving its repression of PIF4 and enhancing thermomorphogenesis.These findings identify the ELF3–BBX24/BBX25–PIF4 axis as a key regulatory module controlling plant growth and development in response to temperature cues.
基金supported by grants from the National Natural Science Foundation of China(32360766,32072595 and 32202512)the Earmarked Fund for CARS(CARS-23-A13)。
文摘CONSTANS(CO)and CONSTANS-LIKE(COL)transcription factors are known to regulate a series of cellular processes,including the transition from vegetative growth to flower development in plants.However,their role in regulating the fruit chlorophyll content is poorly understood.In this study,SlCOL1,the tomato(Solanum lycopersicum)ortholog of Arabidopsis CONSTANS,was shown to play key roles in controlling fruit chlorophyll.The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit,while the overexpression of SlCOL1 increased it.An analysis of protein-protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE(GLK2),which promotes the stability of its protein.The overexpression of SlCOL1in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit,which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content.These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.
