由于用大肠杆菌对植物乳杆菌来源的bsh基因进行胞内和胞外表达时,它们很容易形成包涵体,而且大肠杆菌的内毒素会显限制BSH的应用,所以利用毕赤酵母这一高效的表达系统对其进行了分泌表达.首先用融合PCR的方法将信号肽α-factor与BSH连接...由于用大肠杆菌对植物乳杆菌来源的bsh基因进行胞内和胞外表达时,它们很容易形成包涵体,而且大肠杆菌的内毒素会显限制BSH的应用,所以利用毕赤酵母这一高效的表达系统对其进行了分泌表达.首先用融合PCR的方法将信号肽α-factor与BSH连接,然后再克隆到质粒pPIC9K上,得到重组质粒pPIC9K-α-factor-BSH.重组质粒经过SalⅠ线性化后转化至毕赤酵母GS115.表达产物经SDS-PAGE分析和酶活测定发现,重组BSH的相对分子质量(Mr)和胞外酶活分别为37.0×103和1.08 U mL-1.通过正交实验[L9(34)]对发酵条件优化后,胞外总酶活达到2.69 U mL-1,比原来提高了1.49倍.研究为胆盐水解酶的分离纯化、酶学性质研究以及大规模生产奠定了一定的基础.展开更多
On October 13,the second China-CELAC High-Level Academic Forum cum the sixth China-Latin America and the Caribbean Think Tank Forum was held.Co-hosted by the Institute of Latin American Studies of Chinese Academy of S...On October 13,the second China-CELAC High-Level Academic Forum cum the sixth China-Latin America and the Caribbean Think Tank Forum was held.Co-hosted by the Institute of Latin American Studies of Chinese Academy of Social Sciences(CASS),the Bureau of International Cooperation of CASS,the United Nations Economic Commission for Latin America and the Caribbean(ECLAC),the Chinese People’s Institute of Foreign Affairs(CPIFA),China Institute of International Studies(CIIS)and China Foundation for International Studies(CFIS),the forum was attended by more than 30 experts and scholars from China and Latin American countries,including former Chilean President Eduardo Frei Ruiz-Tagle and ECLAC Executive Secretary Alicia Bárcena.In his remarks at the forum’s closing ceremony,CIIS President Xu Bu expressed the institute’s willingness to contribute to China-Latin America community with a shared future by intellectual exchanges and cooperation.展开更多
January 15-17,2025,Nassau,Bahamas The 3rd Annual CIRT-BS Cybersecurity Conference+15th ITU Caribbean CyberDrill is an event co-organized by the ITU's Telecommunication Development Sector (ITU-D) and the Government...January 15-17,2025,Nassau,Bahamas The 3rd Annual CIRT-BS Cybersecurity Conference+15th ITU Caribbean CyberDrill is an event co-organized by the ITU's Telecommunication Development Sector (ITU-D) and the Government of the Bahamas.The 3rd Annual CIRT-BS Cybersecurity Conference and ITU Regional CyberDrill (formerly Stakeholder Engagement Symposium),under the theme,"Building Shared Cyber Resilience",aims to enhance national and regional cybersecurity partnerships.展开更多
文摘由于用大肠杆菌对植物乳杆菌来源的bsh基因进行胞内和胞外表达时,它们很容易形成包涵体,而且大肠杆菌的内毒素会显限制BSH的应用,所以利用毕赤酵母这一高效的表达系统对其进行了分泌表达.首先用融合PCR的方法将信号肽α-factor与BSH连接,然后再克隆到质粒pPIC9K上,得到重组质粒pPIC9K-α-factor-BSH.重组质粒经过SalⅠ线性化后转化至毕赤酵母GS115.表达产物经SDS-PAGE分析和酶活测定发现,重组BSH的相对分子质量(Mr)和胞外酶活分别为37.0×103和1.08 U mL-1.通过正交实验[L9(34)]对发酵条件优化后,胞外总酶活达到2.69 U mL-1,比原来提高了1.49倍.研究为胆盐水解酶的分离纯化、酶学性质研究以及大规模生产奠定了一定的基础.
文摘On October 13,the second China-CELAC High-Level Academic Forum cum the sixth China-Latin America and the Caribbean Think Tank Forum was held.Co-hosted by the Institute of Latin American Studies of Chinese Academy of Social Sciences(CASS),the Bureau of International Cooperation of CASS,the United Nations Economic Commission for Latin America and the Caribbean(ECLAC),the Chinese People’s Institute of Foreign Affairs(CPIFA),China Institute of International Studies(CIIS)and China Foundation for International Studies(CFIS),the forum was attended by more than 30 experts and scholars from China and Latin American countries,including former Chilean President Eduardo Frei Ruiz-Tagle and ECLAC Executive Secretary Alicia Bárcena.In his remarks at the forum’s closing ceremony,CIIS President Xu Bu expressed the institute’s willingness to contribute to China-Latin America community with a shared future by intellectual exchanges and cooperation.
文摘January 15-17,2025,Nassau,Bahamas The 3rd Annual CIRT-BS Cybersecurity Conference+15th ITU Caribbean CyberDrill is an event co-organized by the ITU's Telecommunication Development Sector (ITU-D) and the Government of the Bahamas.The 3rd Annual CIRT-BS Cybersecurity Conference and ITU Regional CyberDrill (formerly Stakeholder Engagement Symposium),under the theme,"Building Shared Cyber Resilience",aims to enhance national and regional cybersecurity partnerships.
