背景:人尿源性干细胞是近年来新发现的成体干细胞,其来源不受限制,提取简便,具备良好的增殖能力及多向分化潜能,近年来已应用于泌尿系疾病中的神经功能修复,如应激性尿失禁以及膀胱输尿管返流等。目的:探索人尿源性干细胞向神经元样细...背景:人尿源性干细胞是近年来新发现的成体干细胞,其来源不受限制,提取简便,具备良好的增殖能力及多向分化潜能,近年来已应用于泌尿系疾病中的神经功能修复,如应激性尿失禁以及膀胱输尿管返流等。目的:探索人尿源性干细胞向神经元样细胞诱导分化能力及对大鼠脊髓损伤的修复作用。方法:体外获取人尿源性干细胞后利用流式细胞仪检测其细胞表型,将人尿源性干细胞向神经元样细胞诱导后进行免疫组织化学染色鉴定。采用Allen方法制作大鼠T9节段脊髓损伤模型,24只SD大鼠被随机分为2组:脊髓损伤组和人尿源性干细胞组,每组12只。人尿源性干细胞组在脊髓损伤后第1天于损伤脊髓边缘注入2μL细胞浓度为1.0×10^11L^-1人尿源性干细胞,脊髓损伤组注入等量含体积分数为10%胎牛血清的L-DMEM培养液,于造模后第1,10,20,30天进行BBB评分,第30天取各组损伤脊髓组织分别进行Luxol Fast Blue染色、小胶质细胞/巨噬细胞染色和胶质纤维酸性蛋白染色,并计算损伤脊髓面积和胶质纤维酸性蛋白荧光强度。结果与结论:①人尿源性干细胞高表达CD29、CD90,而低表达CD45,并且在体外可向神经元样细胞诱导分化;②造模后第1,10天两组大鼠BBB评分差异无显著性意义(P>0.05),第20,30天人尿源性干细胞组BBB评分明显高于脊髓损伤组(P<0.05);③人尿源性干细胞组脊髓损伤面积明显低于脊髓损伤组(P<0.05),胶质纤维酸性蛋白染色显示人尿源性干细胞组荧光强度明显低于脊髓损伤组(P<0.05);④结果表明,人尿源性干细胞能向神经元样细胞分化,并对大鼠脊髓损伤具有修复作用。展开更多
OBJECTIVE： To evaluate the effects of olfactory ensheathing cell transplantation on functional recovery of rats with complete spinal cord transection. DATA SOURCES： A computer-based online search of Medline （1989-2...OBJECTIVE： To evaluate the effects of olfactory ensheathing cell transplantation on functional recovery of rats with complete spinal cord transection. DATA SOURCES： A computer-based online search of Medline （1989-2013）, Embase （1989- 2013）, Cochrane library （1989-2013）, Chinese Biomedical Literature Database （1989-2013）, China National Knowledge Infrastructure （1989-2013）, VIP （1989-2013）, Wanfang databases （1989-2013） and Chinese Clinical Trial Register was conducted to collect randomized controlled trial data regarding olfactory ensheathing cell transplantation for the treatment of complete spinal cord transection in rats. SELECTION CRITERIA： Randomized controlled trials investigating olfactory ensheathing cell transplantation and other transplantation methods for promoting neurological functional recov- ery of rats with complete spinal cord transection were included in the analysis. Meta analysis was conducted using RevMan 4.2.2 software. MAIN OUTCOME MEASURES： Basso, Beattie and Bresnahan scores of rats with complete spinal cord transection were evaluated in this study. RESULTS： Six randomized controlled trials with high quality methodology were included. Meta analysis showed that Basso, Beattie and Bresnahan scores were significantly higher in the olfacto- ry ensheathing cell transplantation group compared with the control group （WMD = 3.16, 95% （21 （1.68, 4.65）; P 〈 0.00001）. CONCLUSION： Experimental studies have shown that olfactory ensheathing cell transplantation can promote the functional recovery of motor nerves in rats with complete spinal cord transection.展开更多
文摘背景:人尿源性干细胞是近年来新发现的成体干细胞,其来源不受限制,提取简便,具备良好的增殖能力及多向分化潜能,近年来已应用于泌尿系疾病中的神经功能修复,如应激性尿失禁以及膀胱输尿管返流等。目的:探索人尿源性干细胞向神经元样细胞诱导分化能力及对大鼠脊髓损伤的修复作用。方法:体外获取人尿源性干细胞后利用流式细胞仪检测其细胞表型,将人尿源性干细胞向神经元样细胞诱导后进行免疫组织化学染色鉴定。采用Allen方法制作大鼠T9节段脊髓损伤模型,24只SD大鼠被随机分为2组:脊髓损伤组和人尿源性干细胞组,每组12只。人尿源性干细胞组在脊髓损伤后第1天于损伤脊髓边缘注入2μL细胞浓度为1.0×10^11L^-1人尿源性干细胞,脊髓损伤组注入等量含体积分数为10%胎牛血清的L-DMEM培养液,于造模后第1,10,20,30天进行BBB评分,第30天取各组损伤脊髓组织分别进行Luxol Fast Blue染色、小胶质细胞/巨噬细胞染色和胶质纤维酸性蛋白染色,并计算损伤脊髓面积和胶质纤维酸性蛋白荧光强度。结果与结论:①人尿源性干细胞高表达CD29、CD90,而低表达CD45,并且在体外可向神经元样细胞诱导分化;②造模后第1,10天两组大鼠BBB评分差异无显著性意义(P>0.05),第20,30天人尿源性干细胞组BBB评分明显高于脊髓损伤组(P<0.05);③人尿源性干细胞组脊髓损伤面积明显低于脊髓损伤组(P<0.05),胶质纤维酸性蛋白染色显示人尿源性干细胞组荧光强度明显低于脊髓损伤组(P<0.05);④结果表明,人尿源性干细胞能向神经元样细胞分化,并对大鼠脊髓损伤具有修复作用。
文摘OBJECTIVE： To evaluate the effects of olfactory ensheathing cell transplantation on functional recovery of rats with complete spinal cord transection. DATA SOURCES： A computer-based online search of Medline （1989-2013）, Embase （1989- 2013）, Cochrane library （1989-2013）, Chinese Biomedical Literature Database （1989-2013）, China National Knowledge Infrastructure （1989-2013）, VIP （1989-2013）, Wanfang databases （1989-2013） and Chinese Clinical Trial Register was conducted to collect randomized controlled trial data regarding olfactory ensheathing cell transplantation for the treatment of complete spinal cord transection in rats. SELECTION CRITERIA： Randomized controlled trials investigating olfactory ensheathing cell transplantation and other transplantation methods for promoting neurological functional recov- ery of rats with complete spinal cord transection were included in the analysis. Meta analysis was conducted using RevMan 4.2.2 software. MAIN OUTCOME MEASURES： Basso, Beattie and Bresnahan scores of rats with complete spinal cord transection were evaluated in this study. RESULTS： Six randomized controlled trials with high quality methodology were included. Meta analysis showed that Basso, Beattie and Bresnahan scores were significantly higher in the olfacto- ry ensheathing cell transplantation group compared with the control group （WMD = 3.16, 95% （21 （1.68, 4.65）; P 〈 0.00001）. CONCLUSION： Experimental studies have shown that olfactory ensheathing cell transplantation can promote the functional recovery of motor nerves in rats with complete spinal cord transection.
