AIM:To investigate the effects of the probiotic Bifidobacterium longum BB536 on the health management of elderly patients receiving enteral feeding.METHODS:Two double-blind,placebo-controlled trials were performed wit...AIM:To investigate the effects of the probiotic Bifidobacterium longum BB536 on the health management of elderly patients receiving enteral feeding.METHODS:Two double-blind,placebo-controlled trials were performed with long-term inpatients receiving enteral tube feeding at Kitakyushu Hospital Group,Fukuoka,Japan.BB536 was administered as BB536-L and BB536-H powders that contained approximately 2.5 × 10 10 and 5 × 10 10 cfu of BB536,respectively.In the first trial,83 patients(age range:67-101 years) were randomized into 2 groups that received placebo(placebo group) or BB536-H(BB536 group) powders.In the second trial,123 patients(age range:65-102years) were randomized into 3 groups,and each group received placebo(placebo group),BB536-L(BB536-L group),or BB536-H(BB536-H group) powders.Each patient received the study medication for 16 wk after 1 wk of pre-observation.Fecal samples were collected from each patient prior to and after the intervention during Trial 2.Clinical observations included body temperature,occurrence of infection,frequency of defecation,and fecal microbiota.RESULTS:No significant changes were observed in the frequency of defecation for either treatment in Trial 1.However,a significant change was noted in the BB536-L group(P = 0.0439) in Trial 2 but not in the placebo or BB536-H groups.Subgroup analyses based on the frequency of defecation for each patient during the pre-observation period for both trials revealed significant increases in bowel movements in patients with a low frequency of defecation and significant decreases in the bowel movements of patients with a high frequency of defecation during the intervention period in the BB536 groups.The combination of Trials 1 and 2 data revealed a modulatory effect of BB536 ingestion on the changes in bowel movements.Significantly increased bowel movements were observed in patients in the low frequency subgroup with significant intergroup differences(P < 0.01).Significantly decreased bowel movements were observed in patients in the high subgroup,but no significant intergroup differences were observed compared with the placebo group.BB536 ingestion increased the prevalence of normally formed stools.BB536 intake also significantly(P < 0.01) increased the cell numbers of bifidobacteria in fecal microbiota,and significant intergroup differences were observed at week 16.No adverse events were reported in any group.CONCLUSION:Our results suggest that BB536 ingestion modulated the intestinal environment and may have improved the health care of elderly patients receiving enteral feeding.展开更多
Probiotics have numerous biological functions and clinical studies have reported that consuming probiotic foods could promote hair growth and hair follicle health.Bifidobacterium longum BB536 was selected to investiga...Probiotics have numerous biological functions and clinical studies have reported that consuming probiotic foods could promote hair growth and hair follicle health.Bifidobacterium longum BB536 was selected to investigate the possibility of treating hair loss with Cell-Free Supernatant(CFS)of B.longum BB536 and the alleviation mech-anism of CFS on dihydrotestosterone(DHT)-damaged human dermal papilla cells(HDPCs).The results showed that HDPCs treated with 10%CFS of B.longum BB536 for 24 h could significantly promote cell proliferation,repair cell damage,accelerate the process of cell cycle and increase the secretion of VEGF.The CFS of B.longum BB536 reduced the apoptosis rate of DHT-damaged HDPCs.In addition,it could reduce the level of pro-inflammatory factors and improve the alkaline phosphatase(ALP)activity.Metabolites of B.longum BB536 may increase the protein expression of Wnt10b,β-catenin and LEF-1 by activating Wnt/β-catenin signaling pathway and inhibit the expression of DHT-induced negative feedback factors DKK1 and GSK-3β,which alle-viates the damage of DHT on HDPCs,some protein expression levels such as LEF-1 are close to minoxidil(common drugs for hair loss).So,metabolites of B.longum BB536 can be used as new treatment for hair loss due to its safety.This study provides theoretical basis for probiotics to prevent androgenic alopecia.展开更多
文摘AIM:To investigate the effects of the probiotic Bifidobacterium longum BB536 on the health management of elderly patients receiving enteral feeding.METHODS:Two double-blind,placebo-controlled trials were performed with long-term inpatients receiving enteral tube feeding at Kitakyushu Hospital Group,Fukuoka,Japan.BB536 was administered as BB536-L and BB536-H powders that contained approximately 2.5 × 10 10 and 5 × 10 10 cfu of BB536,respectively.In the first trial,83 patients(age range:67-101 years) were randomized into 2 groups that received placebo(placebo group) or BB536-H(BB536 group) powders.In the second trial,123 patients(age range:65-102years) were randomized into 3 groups,and each group received placebo(placebo group),BB536-L(BB536-L group),or BB536-H(BB536-H group) powders.Each patient received the study medication for 16 wk after 1 wk of pre-observation.Fecal samples were collected from each patient prior to and after the intervention during Trial 2.Clinical observations included body temperature,occurrence of infection,frequency of defecation,and fecal microbiota.RESULTS:No significant changes were observed in the frequency of defecation for either treatment in Trial 1.However,a significant change was noted in the BB536-L group(P = 0.0439) in Trial 2 but not in the placebo or BB536-H groups.Subgroup analyses based on the frequency of defecation for each patient during the pre-observation period for both trials revealed significant increases in bowel movements in patients with a low frequency of defecation and significant decreases in the bowel movements of patients with a high frequency of defecation during the intervention period in the BB536 groups.The combination of Trials 1 and 2 data revealed a modulatory effect of BB536 ingestion on the changes in bowel movements.Significantly increased bowel movements were observed in patients in the low frequency subgroup with significant intergroup differences(P < 0.01).Significantly decreased bowel movements were observed in patients in the high subgroup,but no significant intergroup differences were observed compared with the placebo group.BB536 ingestion increased the prevalence of normally formed stools.BB536 intake also significantly(P < 0.01) increased the cell numbers of bifidobacteria in fecal microbiota,and significant intergroup differences were observed at week 16.No adverse events were reported in any group.CONCLUSION:Our results suggest that BB536 ingestion modulated the intestinal environment and may have improved the health care of elderly patients receiving enteral feeding.
基金This work was supported by the CIFST-Yili Foundation of Health Science(2021-Y05).
文摘Probiotics have numerous biological functions and clinical studies have reported that consuming probiotic foods could promote hair growth and hair follicle health.Bifidobacterium longum BB536 was selected to investigate the possibility of treating hair loss with Cell-Free Supernatant(CFS)of B.longum BB536 and the alleviation mech-anism of CFS on dihydrotestosterone(DHT)-damaged human dermal papilla cells(HDPCs).The results showed that HDPCs treated with 10%CFS of B.longum BB536 for 24 h could significantly promote cell proliferation,repair cell damage,accelerate the process of cell cycle and increase the secretion of VEGF.The CFS of B.longum BB536 reduced the apoptosis rate of DHT-damaged HDPCs.In addition,it could reduce the level of pro-inflammatory factors and improve the alkaline phosphatase(ALP)activity.Metabolites of B.longum BB536 may increase the protein expression of Wnt10b,β-catenin and LEF-1 by activating Wnt/β-catenin signaling pathway and inhibit the expression of DHT-induced negative feedback factors DKK1 and GSK-3β,which alle-viates the damage of DHT on HDPCs,some protein expression levels such as LEF-1 are close to minoxidil(common drugs for hair loss).So,metabolites of B.longum BB536 can be used as new treatment for hair loss due to its safety.This study provides theoretical basis for probiotics to prevent androgenic alopecia.
