Probiotics are crucial to human health,but their low survival rate during food processing and application re-mains a significant challenge that needs to be addressed.In this study,the carboxymethyl chitosan-ellagic ac...Probiotics are crucial to human health,but their low survival rate during food processing and application re-mains a significant challenge that needs to be addressed.In this study,the carboxymethyl chitosan-ellagic acid graft(CMCS-EA)and Ca^(2+)were self-assembled to form metal-phenol networks(MPN).The MPN was then used to efficiently encapsulate the anaerobic bacteria(Bifidobacterium animalis subsp.animalis BB04),with the aim of significantly enhancing its resistance to harsh environmental conditions(such as gastric acid,bile salts,and low temperatures)and their probiotic properties.Results showed that MPN exhibited excellent resistance to gastric acid degradation,effectively shielding B.animalis subsp.animalis BB04 from damage caused by gastric acid,bile salts,and low temperatures,thereby significantly enhancing its survival rate,and showed better cell adhesion ability.Based on this,the encapsulated B.animalis subsp.animalis BB04 was incorporated into probiotic ice cream.BB04@CMCS-EA-Ca^(2+)not only significantly reduced the ice cream's overrun rate and melting rate,more importantly,maintained B.animalis subsp.animalis BB04′s viability at a stable level throughout a 180-day storage period at-20℃,keeping its live bacterial count consistently above 6.0 log CFU/g.This study not only provides an efficient and multifunctional encapsulation strategy for delivering high-viability probiotics but also demonstrates its significant application potential in developing stable frozen probiotic functional foods.展开更多
基金funded by Beijing Natural Science Foundation(No.6252001)Natural Science Foundation of China(No.31871772).
文摘Probiotics are crucial to human health,but their low survival rate during food processing and application re-mains a significant challenge that needs to be addressed.In this study,the carboxymethyl chitosan-ellagic acid graft(CMCS-EA)and Ca^(2+)were self-assembled to form metal-phenol networks(MPN).The MPN was then used to efficiently encapsulate the anaerobic bacteria(Bifidobacterium animalis subsp.animalis BB04),with the aim of significantly enhancing its resistance to harsh environmental conditions(such as gastric acid,bile salts,and low temperatures)and their probiotic properties.Results showed that MPN exhibited excellent resistance to gastric acid degradation,effectively shielding B.animalis subsp.animalis BB04 from damage caused by gastric acid,bile salts,and low temperatures,thereby significantly enhancing its survival rate,and showed better cell adhesion ability.Based on this,the encapsulated B.animalis subsp.animalis BB04 was incorporated into probiotic ice cream.BB04@CMCS-EA-Ca^(2+)not only significantly reduced the ice cream's overrun rate and melting rate,more importantly,maintained B.animalis subsp.animalis BB04′s viability at a stable level throughout a 180-day storage period at-20℃,keeping its live bacterial count consistently above 6.0 log CFU/g.This study not only provides an efficient and multifunctional encapsulation strategy for delivering high-viability probiotics but also demonstrates its significant application potential in developing stable frozen probiotic functional foods.
