Background and Objectives: The distal radius fracture (DRF) is a major public health problem in northern countries. Its frequency is constantly increasing. The locked anterior plate with its well-established biomechan...Background and Objectives: The distal radius fracture (DRF) is a major public health problem in northern countries. Its frequency is constantly increasing. The locked anterior plate with its well-established biomechanical properties, offers a reliable alternative. The aim of this study was to evaluate the radiological, the functional results and to determine the factors of poor postoperative prognosis of DRF treated with Newclip radial plates®. Methodology: This prospective cohort study evaluates the radiological and functional outcames of displaced radius fractures (DRFs) in patients ≥50 years old treated with Newclip® (locked anterior plates) at the Basse-Terre Hospital in Guadeloupe from 2022 to 2024. The patients were categorized into those with epiphyseal involvement (E1 - E4) and without epiphyseal involvement (E0) based on Laulan’s MEU classification. Radiological parameters (distal radio-ulnar index (DRUI), radial inclination frant view (IRF), radial inclination sagittal view (IRS) were assessed pre and post-operatively. Functional recovery was evaluated at 12 months using the QuickDash questionnaire. Results: Falls were the most common cause of fracture. Post-operatively, SRI was the least restored parameter. Poor prognostic factor for SRI improvement included posterior commimution and unstable fractures. Factors associated with higher QuickDash scores included unstable factures, unrestored DRUI, low plate position, metaphyseal features, and ulnar features. Conclusion: The anterior locking plate osteosynthesis is reliable treatment option with excellent functional outcomes.展开更多
Urea is a major end product of nitrogen catabolism,serving as an osmolyte to regulate osmotic stress in fish exposed to varying water environments.It has been well known that urea transporters(UTs)facilitate the rapid...Urea is a major end product of nitrogen catabolism,serving as an osmolyte to regulate osmotic stress in fish exposed to varying water environments.It has been well known that urea transporters(UTs)facilitate the rapid movement of urea across cell membranes.However,researches on ut genes were predominantly focused on elasmobranchs and early developmental stages of fish.In this investigation,a total of three ut genes were identified in spotted sea bass.Phylogenetic,homology,and syntenic analyses were conducted to validate the annotation and assess the evolutionary relationships among ut genes.Both ut-a and ut-b genes have retained their evolutionary stability,demonstrating a significant level of homology between them.To gain deeper insights into the evolution of ut genes in spotted sea bass,we performed selective pressure analysis using site,branch,and branch-site models.The results suggested that positive selection likely played a significant role in shaping the evolution of the ut gene family.Furthermore,tissue-specific expression analyses revealed high expression levels of ut genes in osmoregulatory tissues such as the gill and kidney.Additionally,all three ut genes exhibited salinity-related expression patterns in gill and kidney tissues during both seawater-to-freshwater(SF)and freshwater-to-seawater(FS)adaptation.In situ hybridization results demonstrated the localization of both ut-a and ut-c mRNAs on the gill lamellae and adjacent gill filament epithelium.In summary,our study establishes a solid foundation for future research elucidating the evolutionary relationships and functional significance of ut genes during salinity acclimation in spotted sea bass and other teleost species.展开更多
Deep-sea aquaculture is an emerging trend due to the contamination and overexploitation of nearshore mariculture areas.However,the complex water conditions in the deep sea impose higher demands on the swimming perform...Deep-sea aquaculture is an emerging trend due to the contamination and overexploitation of nearshore mariculture areas.However,the complex water conditions in the deep sea impose higher demands on the swimming performance of farmed animals.Spotted sea bass(Lateolabrax maculatus)is one of the most economically important fish species in China.To investigate the mechanisms underlying the individual variations in swimming performance among spotted sea bass,we measured their critical swimming speed(U_(crit))and morphological phenotypes.Total length,body length,body weight,caudal region length,and condition factor showed significant positive correlations with absolute Ucrit.In contrast,caudal fin length and pectoral fin length tended to hinder the swimming performance of individual spotted sea bass.Additionally,white muscle tissues from fish exhibiting good swimming performance(relative U_(crit)>8.20 BL/s)and poor swimming performance(relative U_(crit)<7.31 BL/s)were sampled for RNA-seq.A total of 694 differential expression genes(DEGs)were identified through gene expression analysis,with significant enrichment in GO terms such as mitochondrial protein complex,ribosomal subunit,structural constituent of ribosome,and oxidative phosphorylation,as well as genes in KEGG pathways including ribosome and metabolic pathways.In conclusion,our study for the first time comprehensively elucidated the impact of morphology of spotted sea bass on its individual differences in swimming ability,and analyzed the genetic basis underlying swimming ability using transcriptomic methods.This study provides a theoretical basis for the potential breeding varieties of spotted sea bass suitable for deep-sea aquaculture.展开更多
With the large-scale promotion of distributed photovoltaics,new challenges have emerged in the photovoltaic consumptionwithin distribution networks.Traditional photovoltaic consumption schemes have primarily focused o...With the large-scale promotion of distributed photovoltaics,new challenges have emerged in the photovoltaic consumptionwithin distribution networks.Traditional photovoltaic consumption schemes have primarily focused on static analysis.However,as the scale of photovoltaic power generation devices grows and the methods of integration diversify,a single consumption scheme is no longer sufficient to meet the actual needs of current distribution networks.Therefore,this paper proposes an optimal evaluation method for photovoltaic consumption schemes based on BASS model predictions of installed capacity,aiming to provide an effective tool for generating and evaluating photovoltaic consumption schemes in distribution networks.First,the BASS diffusion model,combined with existing photovoltaic capacity data and roof area information,is used to predict the trends in photovoltaic installed capacity for each substation area,providing a scientific basis for consumption evaluation.Secondly,an improved random scenario simulation method is proposed for assessing the photovoltaic consumption capacity in distribution networks.This method generates photovoltaic integration schemes based on the diffusion probabilities of different regions and evaluates the consumption capacity of each scheme.Finally,the Technique for Order Preference by Similarity to an Ideal Solution(TOPSIS)is used to comprehensively evaluate the generated schemes,ensuring that the selected scheme not only meets the consumption requirements but also offers high economic benefits and reliability.The effectiveness and feasibility of the proposedmethod are validated through simulations of the IEEE 33-node system,providing strong support for optimizing photovoltaic consumption schemes in distribution networks.展开更多
Background The carnivorous fish,largemouth bass(Micropterus salmoides),has difficulty metabolizing dietary carbohydrates,frequently resulting in issues with energy metabolism and fatty liver disease.Nevertheless,the m...Background The carnivorous fish,largemouth bass(Micropterus salmoides),has difficulty metabolizing dietary carbohydrates,frequently resulting in issues with energy metabolism and fatty liver disease.Nevertheless,the molecular mechanisms involved are still not fully understood.Results The results of high-carbohydrate(HC)diets and high-glucose(HG)treatments in largemouth bass hepatocytes showed that high-glucose causes liver damage and glycolipid accumulation.High-glucose promoted the lipogenesis process by activating AMPK/ACC/SREBP-1 pathway and reduced bile acid synthesis by downregulating cholesterol 7-hydroxylase(cyp7a1)and sterol 12-hydroxylase(cyp8b1).Concurrently,HG treatments also caused mitochondrial fission and damage by increasing the expression of dynamin-related protein 1(Drp1),leading to impaired mitochondria accumulation and mitochondria-dependent apoptosis via the p38 MAPK/Bcl-2/Casp3 pathway.Additionally,HG treatments decreased Sirt1 expression and relocated it from the nucleus to the cytoplasm,where it interacts with autophagosomes and lysosomes,inhibiting Pink1/Parkin-mediated mitophagy.This also led to the cytoplasmic translocation of Pink1 and its co-localization with Sirt1,indicating that Sirt1 regulates high glucose-induced metabolic stress by inhibiting the Pink1/Parkin mitophagy pathway.Conclusion In summary,HG treatment induces mitochondrial damage and glycolipid accumulation in largemouth bass through mechanisms involving AMPK/SREBP1/ACC1-mediated lipogenesis,bile acid metabolism,Sirt-mediated mitophagy,and p38 MAPK/Bcl-2/Casp3-activated apoptosis.展开更多
Background To more effectively address the scarcity resources and elevated costs associated with fishmeal(FM),the utilization of cottonseed protein concentrate(CPC)as an alternative in aquaculture feeds has become inc...Background To more effectively address the scarcity resources and elevated costs associated with fishmeal(FM),the utilization of cottonseed protein concentrate(CPC)as an alternative in aquaculture feeds has become increasingly prevalent.However,high levels of CPC substitution for FM have been reported to suppress the growth of fish and impair intestinal health.Hydrolysable tannin(HT)has been reported to exhibit biological activities such as antiinflammatory and antioxidant activities,but whether the HT can generate positive biological effects on the intestinal health of largemouth bass(Micropterus salmoides)remains unknown.Largemouth bass(initial weight:6.03±0.01 g)were subjected to an 8-week feeding trial with three different diets:a basic diet(named as the NC),a high CPC diet(in which CPC replaced 75%of the FM protein in the NC diet,named as the HC),and an additive diet(1.25 g/kg of the HT was added to the HC diet,named as the HCH)to explore the potential benefits of HT on intestinal health.Results The HC treatment significantly reduced the weight gain rate of fish,increased the feed conversion ratio,and induced intestinal inflammation.However,the HCH treatment could alleviate the adverse impacts of the HC diet,as evidenced by the promotion of growth and feed utilization,increased activity of digestive enzymes and antioxidant capacities,downregulated expression of pro-inflammatory factors,and maintenance of the integrity of intestinal barrier.Metabolomic analysis revealed that HCH treatment could reduce the pro-inflammatory active substances produced by arachidonic acid metabolism,including prostaglandin F2α(PGF2α)and leukotriene B4(LTB4).Transcriptomic results indicated that dietary HT might alleviate intestinal inflammation by suppressing the activation of the NF-κB signaling pathway.Furthermore,correlation analysis demonstrated that the metabolites PGF2αand LTB4,derived from arachidonic acid,exhibited a significant positive correlation with the expression of genes associated with proinflammatory responses within the NF-κB signaling pathway.Conclusions The study indicates that the HT mitigates the growth retardation and intestinal inflammation resulting from the HC diet on largemouth bass.展开更多
文摘Background and Objectives: The distal radius fracture (DRF) is a major public health problem in northern countries. Its frequency is constantly increasing. The locked anterior plate with its well-established biomechanical properties, offers a reliable alternative. The aim of this study was to evaluate the radiological, the functional results and to determine the factors of poor postoperative prognosis of DRF treated with Newclip radial plates®. Methodology: This prospective cohort study evaluates the radiological and functional outcames of displaced radius fractures (DRFs) in patients ≥50 years old treated with Newclip® (locked anterior plates) at the Basse-Terre Hospital in Guadeloupe from 2022 to 2024. The patients were categorized into those with epiphyseal involvement (E1 - E4) and without epiphyseal involvement (E0) based on Laulan’s MEU classification. Radiological parameters (distal radio-ulnar index (DRUI), radial inclination frant view (IRF), radial inclination sagittal view (IRS) were assessed pre and post-operatively. Functional recovery was evaluated at 12 months using the QuickDash questionnaire. Results: Falls were the most common cause of fracture. Post-operatively, SRI was the least restored parameter. Poor prognostic factor for SRI improvement included posterior commimution and unstable fractures. Factors associated with higher QuickDash scores included unstable factures, unrestored DRUI, low plate position, metaphyseal features, and ulnar features. Conclusion: The anterior locking plate osteosynthesis is reliable treatment option with excellent functional outcomes.
基金supported by the National Natural Science Foundation of China(No.32072947)the China Agriculture Research System(No.CARS-47)。
文摘Urea is a major end product of nitrogen catabolism,serving as an osmolyte to regulate osmotic stress in fish exposed to varying water environments.It has been well known that urea transporters(UTs)facilitate the rapid movement of urea across cell membranes.However,researches on ut genes were predominantly focused on elasmobranchs and early developmental stages of fish.In this investigation,a total of three ut genes were identified in spotted sea bass.Phylogenetic,homology,and syntenic analyses were conducted to validate the annotation and assess the evolutionary relationships among ut genes.Both ut-a and ut-b genes have retained their evolutionary stability,demonstrating a significant level of homology between them.To gain deeper insights into the evolution of ut genes in spotted sea bass,we performed selective pressure analysis using site,branch,and branch-site models.The results suggested that positive selection likely played a significant role in shaping the evolution of the ut gene family.Furthermore,tissue-specific expression analyses revealed high expression levels of ut genes in osmoregulatory tissues such as the gill and kidney.Additionally,all three ut genes exhibited salinity-related expression patterns in gill and kidney tissues during both seawater-to-freshwater(SF)and freshwater-to-seawater(FS)adaptation.In situ hybridization results demonstrated the localization of both ut-a and ut-c mRNAs on the gill lamellae and adjacent gill filament epithelium.In summary,our study establishes a solid foundation for future research elucidating the evolutionary relationships and functional significance of ut genes during salinity acclimation in spotted sea bass and other teleost species.
基金National Key R&D Program of China(No.2022YFD2400103).
文摘Deep-sea aquaculture is an emerging trend due to the contamination and overexploitation of nearshore mariculture areas.However,the complex water conditions in the deep sea impose higher demands on the swimming performance of farmed animals.Spotted sea bass(Lateolabrax maculatus)is one of the most economically important fish species in China.To investigate the mechanisms underlying the individual variations in swimming performance among spotted sea bass,we measured their critical swimming speed(U_(crit))and morphological phenotypes.Total length,body length,body weight,caudal region length,and condition factor showed significant positive correlations with absolute Ucrit.In contrast,caudal fin length and pectoral fin length tended to hinder the swimming performance of individual spotted sea bass.Additionally,white muscle tissues from fish exhibiting good swimming performance(relative U_(crit)>8.20 BL/s)and poor swimming performance(relative U_(crit)<7.31 BL/s)were sampled for RNA-seq.A total of 694 differential expression genes(DEGs)were identified through gene expression analysis,with significant enrichment in GO terms such as mitochondrial protein complex,ribosomal subunit,structural constituent of ribosome,and oxidative phosphorylation,as well as genes in KEGG pathways including ribosome and metabolic pathways.In conclusion,our study for the first time comprehensively elucidated the impact of morphology of spotted sea bass on its individual differences in swimming ability,and analyzed the genetic basis underlying swimming ability using transcriptomic methods.This study provides a theoretical basis for the potential breeding varieties of spotted sea bass suitable for deep-sea aquaculture.
基金supported in part by theThe Planning Subject Project of Guangdong Power Grid Co.,Ltd.(62273104).
文摘With the large-scale promotion of distributed photovoltaics,new challenges have emerged in the photovoltaic consumptionwithin distribution networks.Traditional photovoltaic consumption schemes have primarily focused on static analysis.However,as the scale of photovoltaic power generation devices grows and the methods of integration diversify,a single consumption scheme is no longer sufficient to meet the actual needs of current distribution networks.Therefore,this paper proposes an optimal evaluation method for photovoltaic consumption schemes based on BASS model predictions of installed capacity,aiming to provide an effective tool for generating and evaluating photovoltaic consumption schemes in distribution networks.First,the BASS diffusion model,combined with existing photovoltaic capacity data and roof area information,is used to predict the trends in photovoltaic installed capacity for each substation area,providing a scientific basis for consumption evaluation.Secondly,an improved random scenario simulation method is proposed for assessing the photovoltaic consumption capacity in distribution networks.This method generates photovoltaic integration schemes based on the diffusion probabilities of different regions and evaluates the consumption capacity of each scheme.Finally,the Technique for Order Preference by Similarity to an Ideal Solution(TOPSIS)is used to comprehensively evaluate the generated schemes,ensuring that the selected scheme not only meets the consumption requirements but also offers high economic benefits and reliability.The effectiveness and feasibility of the proposedmethod are validated through simulations of the IEEE 33-node system,providing strong support for optimizing photovoltaic consumption schemes in distribution networks.
基金supported by Project of National Natural Science Foundation of China(32172982)Project of Science and Technology of Guangdong Province(2021B0202050002)+2 种基金Project of Science and Technology of Guangdong Province(2023A1515012627)National Natural Science Foundation of China(32303014)Guangdong Basic and Applied Basic Research Foundation(2024A1515010444).
文摘Background The carnivorous fish,largemouth bass(Micropterus salmoides),has difficulty metabolizing dietary carbohydrates,frequently resulting in issues with energy metabolism and fatty liver disease.Nevertheless,the molecular mechanisms involved are still not fully understood.Results The results of high-carbohydrate(HC)diets and high-glucose(HG)treatments in largemouth bass hepatocytes showed that high-glucose causes liver damage and glycolipid accumulation.High-glucose promoted the lipogenesis process by activating AMPK/ACC/SREBP-1 pathway and reduced bile acid synthesis by downregulating cholesterol 7-hydroxylase(cyp7a1)and sterol 12-hydroxylase(cyp8b1).Concurrently,HG treatments also caused mitochondrial fission and damage by increasing the expression of dynamin-related protein 1(Drp1),leading to impaired mitochondria accumulation and mitochondria-dependent apoptosis via the p38 MAPK/Bcl-2/Casp3 pathway.Additionally,HG treatments decreased Sirt1 expression and relocated it from the nucleus to the cytoplasm,where it interacts with autophagosomes and lysosomes,inhibiting Pink1/Parkin-mediated mitophagy.This also led to the cytoplasmic translocation of Pink1 and its co-localization with Sirt1,indicating that Sirt1 regulates high glucose-induced metabolic stress by inhibiting the Pink1/Parkin mitophagy pathway.Conclusion In summary,HG treatment induces mitochondrial damage and glycolipid accumulation in largemouth bass through mechanisms involving AMPK/SREBP1/ACC1-mediated lipogenesis,bile acid metabolism,Sirt-mediated mitophagy,and p38 MAPK/Bcl-2/Casp3-activated apoptosis.
基金supported by the National Key Research and Development Program of China(2024YFD1300803).
文摘Background To more effectively address the scarcity resources and elevated costs associated with fishmeal(FM),the utilization of cottonseed protein concentrate(CPC)as an alternative in aquaculture feeds has become increasingly prevalent.However,high levels of CPC substitution for FM have been reported to suppress the growth of fish and impair intestinal health.Hydrolysable tannin(HT)has been reported to exhibit biological activities such as antiinflammatory and antioxidant activities,but whether the HT can generate positive biological effects on the intestinal health of largemouth bass(Micropterus salmoides)remains unknown.Largemouth bass(initial weight:6.03±0.01 g)were subjected to an 8-week feeding trial with three different diets:a basic diet(named as the NC),a high CPC diet(in which CPC replaced 75%of the FM protein in the NC diet,named as the HC),and an additive diet(1.25 g/kg of the HT was added to the HC diet,named as the HCH)to explore the potential benefits of HT on intestinal health.Results The HC treatment significantly reduced the weight gain rate of fish,increased the feed conversion ratio,and induced intestinal inflammation.However,the HCH treatment could alleviate the adverse impacts of the HC diet,as evidenced by the promotion of growth and feed utilization,increased activity of digestive enzymes and antioxidant capacities,downregulated expression of pro-inflammatory factors,and maintenance of the integrity of intestinal barrier.Metabolomic analysis revealed that HCH treatment could reduce the pro-inflammatory active substances produced by arachidonic acid metabolism,including prostaglandin F2α(PGF2α)and leukotriene B4(LTB4).Transcriptomic results indicated that dietary HT might alleviate intestinal inflammation by suppressing the activation of the NF-κB signaling pathway.Furthermore,correlation analysis demonstrated that the metabolites PGF2αand LTB4,derived from arachidonic acid,exhibited a significant positive correlation with the expression of genes associated with proinflammatory responses within the NF-κB signaling pathway.Conclusions The study indicates that the HT mitigates the growth retardation and intestinal inflammation resulting from the HC diet on largemouth bass.
