Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm matura...Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm maturation in the mouse, immunoprecipitated basigin samples from testis and epididymal spermatozoa were analyzed by liquid chromatography-mass spectrometry/mass spectrometry （LC-MS/MS）. The results demonstrated that basigin molecules from the testis and spermatozoa were separable into two major bands and that the differences in the molecular sizes were possibly because of an endoproteolytic cleavage. Since basigin is known to be a chaperone for the monocarboxylate transporter 1 （MCT1）, the localization of basigin, MCT1 and MCT2 was examined during postnatal testicular development. Immunohistochemical studies showed different expression patterns of MCT1 and MCT2. MCT1 was localized on the surface of spermatogonia, spermatocytes, and spermatids. In contrast, MCT2 appeared on the principal piece of spermatozoa in the testis, where basigin was also observed. In mature epididymal spermatozoa, MCT2 was located on the midpiece, where basigin co-localized with MCT2 but not with MCT1. Furthermore, MCT2 was immunoprecipitated with basigin in mouse testes and sperm. These results suggest that basigin has a functional role as a binding partner with MCT2 in testicular and epididymal spermatozoa.展开更多
Hepatocellular carcinoma （HCC） is one of the most common malignancies worldwide, ranking fifth in incidence and second in mortality （1）. Although HCC cases mainly occurred in South-East Asia and Southern Africa in...Hepatocellular carcinoma （HCC） is one of the most common malignancies worldwide, ranking fifth in incidence and second in mortality （1）. Although HCC cases mainly occurred in South-East Asia and Southern Africa in the past, the incidence of this disease has been on the rise in the Western countries over the forty last years （1）. Despite the advances in the diagnostic techniques and novel therapies, HCC remains a tumor with a dismal prognosis （2-4）. While the detection of HCC at an early stage allows the employment of potentially curative treatments, such as liver transplantation, surgical resection and tumor ablation, over two thirds of patients are diagnosed at a late stage of the disease, when conventional therapeutic approaches are ineffective （2-4）. Furthermore, administration of the multikinase inhibitor Sorafenib, the only drug approved by the Food and Drug Administration （FDA） for targeted therapy of advanced HCC, provides only limited benefits to HCC patients in terms of overall survival （2-4）. Thus, in order to improve significantly the effectiveness of therapeutic strategies against liver cancer, a deeper understanding of the molecular pathogenesis of this deadly disease is necessary.展开更多
文摘Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm maturation in the mouse, immunoprecipitated basigin samples from testis and epididymal spermatozoa were analyzed by liquid chromatography-mass spectrometry/mass spectrometry （LC-MS/MS）. The results demonstrated that basigin molecules from the testis and spermatozoa were separable into two major bands and that the differences in the molecular sizes were possibly because of an endoproteolytic cleavage. Since basigin is known to be a chaperone for the monocarboxylate transporter 1 （MCT1）, the localization of basigin, MCT1 and MCT2 was examined during postnatal testicular development. Immunohistochemical studies showed different expression patterns of MCT1 and MCT2. MCT1 was localized on the surface of spermatogonia, spermatocytes, and spermatids. In contrast, MCT2 appeared on the principal piece of spermatozoa in the testis, where basigin was also observed. In mature epididymal spermatozoa, MCT2 was located on the midpiece, where basigin co-localized with MCT2 but not with MCT1. Furthermore, MCT2 was immunoprecipitated with basigin in mouse testes and sperm. These results suggest that basigin has a functional role as a binding partner with MCT2 in testicular and epididymal spermatozoa.
文摘Hepatocellular carcinoma （HCC） is one of the most common malignancies worldwide, ranking fifth in incidence and second in mortality （1）. Although HCC cases mainly occurred in South-East Asia and Southern Africa in the past, the incidence of this disease has been on the rise in the Western countries over the forty last years （1）. Despite the advances in the diagnostic techniques and novel therapies, HCC remains a tumor with a dismal prognosis （2-4）. While the detection of HCC at an early stage allows the employment of potentially curative treatments, such as liver transplantation, surgical resection and tumor ablation, over two thirds of patients are diagnosed at a late stage of the disease, when conventional therapeutic approaches are ineffective （2-4）. Furthermore, administration of the multikinase inhibitor Sorafenib, the only drug approved by the Food and Drug Administration （FDA） for targeted therapy of advanced HCC, provides only limited benefits to HCC patients in terms of overall survival （2-4）. Thus, in order to improve significantly the effectiveness of therapeutic strategies against liver cancer, a deeper understanding of the molecular pathogenesis of this deadly disease is necessary.
