Background: Antibiotics have been used routinely for postoperative care in patients with chronic rhinosinusitis (CRS). However, increased bacterial growth was found after antibiotic treatment. In traditional Chinese m...Background: Antibiotics have been used routinely for postoperative care in patients with chronic rhinosinusitis (CRS). However, increased bacterial growth was found after antibiotic treatment. In traditional Chinese medicine, Jia Wei Cang Er San has been used to treat CRS. This study was to investigate the influence of Jia Wei Cang Er San on the postoperative bacteriology of CRS. Methods: Ninety-seven CRS patients who underwent functional endoscopic sinus surgery (FESS) were included. They were randomly divided into 3 groups. In the group of Chinese herbal medicine (CHM), patients were given a capsule of Jia Wei Cang Er San tid for 8 weeks and a placebo capsule for amoxicillin q8h for 4 weeks after FESS. In the amoxicillin group, patients were given a capsule of amoxicillin 250 mg q8h for 4 weeks and a placebo capsule for Jia Wei Cang Er San tid for 8 weeks. In the placebo group, patients were given both placebo capsules. Bacterial cultures were performed from bilateral middle meati before FESS, and 8 and 12 weeks after FESS. Results: In the CHM group, bacteria grew in 21 (46%) of 46 specimens pre-operatively, in 23 (50%) specimens 8 weeks and in 17 (37%) specimens 12 weeks after surgery. In the amoxicillin group, bacteria grew in 15 (28%) of 54 specimens pre-operatively, in 30 (56%) specimens 8 weeks and in 32 (59%) specimens 12 weeks after surgery. In the placebo group, bacteria were found in 13 (34%) of 38 specimens pre-operatively, in 16 (42%) specimens 8 weeks and in 12 (32%) specimens 12 weeks after surgery. The rates of bacterial growth did not change by Jia Wei Cang Er San 8 or 12 weeks after surgery, but increased significantly by amoxicillin 8 and 12 weeks after surgery. Conclusion: Our study showed that Jia Wei Cang Er San did not induce bacterial growth after FESS as amoxicillin.展开更多
Aquatic Animal Bacteriology is a basic course of Aquatic Animal Medicine discipline.Bacteriology is not only a frontier subject in the field of life sciences,but also one of the most rapidly developing scientific fiel...Aquatic Animal Bacteriology is a basic course of Aquatic Animal Medicine discipline.Bacteriology is not only a frontier subject in the field of life sciences,but also one of the most rapidly developing scientific fields today.The Belt and Road Initiative has brought opportunities and challenges to the upgrading and development of this discipline,so this discipline faces many problems that need to be solved.In view of this,this study combined the development trend of the discipline,reorganized and optimized the course content,and prepared sophisticated multimedia courseware,to stimulate students'learning initiative and enthusiasm,expand the field of professional knowledge,improve their English translation and writing skills,so as to lay a solid foundation for their future service to the Belt and Road Initiative.展开更多
Bacteria are smallest primitive, simple, unicellular, prokaryotic and microscopic organisms. But these organisms cannot be studied with naked eyes because of their minute structure. Therefore in search for the informa...Bacteria are smallest primitive, simple, unicellular, prokaryotic and microscopic organisms. But these organisms cannot be studied with naked eyes because of their minute structure. Therefore in search for the information about the structure and composition of bacterial cells, cell biologist used light microscopes with a numerical aperture of 1.4 and using wavelength of 0.4 μm separation. But there are still certain cellular structures that cannot be seen through naked eyes, and for them electron microscope is used. There are certain improved types of light microscope which can be incorporated to improve their resolving power. Hence microscopy is playing a crucial role in the field of bacteriology.展开更多
The paper examines the response of Chinese medicine and the general public to the introduction of the first-ever synthetic chemical agent of Salvarsan(洒尔佛散)to the Chinese market.Since the new medicine was first in...The paper examines the response of Chinese medicine and the general public to the introduction of the first-ever synthetic chemical agent of Salvarsan(洒尔佛散)to the Chinese market.Since the new medicine was first introduced in 1910,the feedback has been predominantly positive.The rapid efficacy of Salvarsan was also recognised by Chinese medicine practitioners,who integrated it into the Chinese traditional herbal classification system and redefined its pharmacological properties in Chinese medicine theories.Nevertheless,Chinese medicine’s perspective on Salvarsan goes beyond mere acceptance.The discourse surrounding Salvarsan encompasses its therapeutic effectiveness and delves deeper into broader themes concerning the fundamental principles of the medication as well as the dispute between Chinese and Western medicine that transcends medical theory.To maintain the validity of Chinese medicine theory in the discussion that went beyond simple medical theory,Chinese medicine used Salvarsan as evidence against the notion that bacteriological theory was the sole reliable theory of disease.展开更多
Groundwater quality assessment is important to assure safe and durable water use.In semi-arid areas of Algeria,groundwater represents the main water resource for drinking water supply of the rural population as well a...Groundwater quality assessment is important to assure safe and durable water use.In semi-arid areas of Algeria,groundwater represents the main water resource for drinking water supply of the rural population as well as for irrigation of agricultural lands.Groundwater samples from wells and springs were collected from the Gargaat Tarf and Annk Djemel sub-watersheds of the Oum El Bouaghi,Algeria,and were analyzed and compared with the World Health Organization(WHO)standards.Results showed that most of the measured physical and chemical parameters exceeded the quality limits according to the WHO standards.Groundwater had a slightly alkaline water pH(7.00-7.79),electrical conductivity>1500μS/cm,chloride>500 mg/L,calcium>250 mg/L,and magnesium>155 mg/L.Water quality index(WQI)results showed that 68%of the area had excellent water quality,24%of the samples fell into good category,and only 8%were of poor quality and unsuitable for human consumption.Six wells in the area showed bacterial contamination.Total coliforms(453.9(±180.3)CFU(colony-forming units)/100 mL),fecal coliforms(243.2(±99.2)CFU/100 mL),and fecal streptococci(77.9(±32.0)CFU/100 mL)loads were above the standard limits set by the WHO.These results confirmed that water resources in the study area were strongly influenced by anthropogenic activities and were not recommended for consumption as drinking water.展开更多
Background: Despite a significant decline in neonatal deaths in the last 20 years (5 million in 1990 to 2.4 million in 2019), the risk of death is still high, especially in developing countries. In Tanzania, neonatal ...Background: Despite a significant decline in neonatal deaths in the last 20 years (5 million in 1990 to 2.4 million in 2019), the risk of death is still high, especially in developing countries. In Tanzania, neonatal sepsis is the third leading cause of neonatal death, accounting for 25% of all deaths. The rising global threat of antimicrobial resistance and the rising burden of neonatal death due to neonatal sepsis have been of great concern and have delayed progress toward reaching SDG goal 3.2 by 2030. This study aims to determine the bacteriological profile, antibiotic susceptibility patterns, and predictors of bacteremia among neonates with clinical sepsis at KCMC Hospital in Northern Tanzania. Methodology: This study had a cross-sectional design conducted at KCMC Hospital, Northern Tanzania. The study population was neonates admitted to the neonatal unit at KCMC Hospital. Data were collected using questionnaires and blood cultures from neonates. Frequencies and proportions were used to summarize categorical variables, while continuous variables were summarized using mean and standard deviation. The frequencies and proportions of bacteria isolated and the antimicrobial susceptibility results were analyzed and compared using Pearson’s chi-square test and Fisher’s exact test where applicable. Modified Poisson regression model was used to determine factors associated with positive blood culture. Results: Out of 411 neonates with a clinical diagnosis of neonatal sepsis, 175 (42.9%) had positive blood cultures. Gram-positive bacteria were most frequently isolated at 52.3%, and gram-negative bacteria were 47.7%. Coagulase-negative Staphylococcus (30.7%) and Staphylococcus aureus (19.9%) were the predominant gram-positive isolates. Gram-negative isolates were Klebsiella spp 47 (26.7%), E. coli 10 (5.7%), and Citrobacter spp 10 (5.1%). The gram-positive isolates were sensitive to vancomycin, piperacillin/tazobactam, and ceftazidime, whereas the gram-negative were sensitive to amikacin, meropenem, and vancomycin. The study did not find statistically significant associations between clinical factors and positive blood cultures in bacteremia. Conclusion: Gram-positive bacteria are the dominant pathogens in early-onset and late-onset neonatal sepsis. High levels of resistance to ampicillin and ceftriaxone and moderate resistance to gentamycin were observed in both gram-positive and gram-negative bacteria. Gram-positive organisms exhibit better susceptibility rates to vancomycin and ciprofloxacin, while gram-negative micro-organisms are more sensitive to amikacin and meropenem. An effective initial treatment approach for neonatal sepsis would involve a combination of drugs.展开更多
Introduction: Microbiology of effusion fluids in children in Burkina Faso is characterized by the scarcity of data. This work aimed to study the bacteriological and antibiotics susceptibility profile of bacteria invol...Introduction: Microbiology of effusion fluids in children in Burkina Faso is characterized by the scarcity of data. This work aimed to study the bacteriological and antibiotics susceptibility profile of bacteria involved in effusion fluid infections in paediatrics in order to improve the choice of probabilistic antibiotics therapy. Methods: A cross-sectional, descriptive study was used in children aged 0 to 15 years from 2017 to 2020 at the Charles De Gaulle Pediatric University Hospital Center (CHUP-CDG) in Ouagadougou. Classical bacteriology methods such as macroscopy, Gram staining, identification galleries and antibiotics susceptibility testing were used. Results: Of 231 samples, 64 bacteria were isolated. The most common bacterial strains of pleural fluid were Staphylococcus aureus (25%) and 40% for Enterobacteriaceae. Of the peritoneal fluid, 77% were Enterobacteriaceae with 57% Escherichia coli;and from joint fluid, 33% were S. aureus and 22% for P. aeruginosa. The overall susceptibility profile showed 29% extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL), 10% methicillin-resistant S. aureus (MRSA), and 8% carbapenemases. Conclusion: Bacteriological profile is characterized by ESBL-producing Enterobacteriaceae and MRSA. The most active antibiotics were macrolides, aminoglycosides, and cefoxitin (methicillin) for Gram-positive cocci, carbapenems, and aminoglycosides for Gram-negative bacilli. Then, the monitoring of antibiotics resistance must be permanent.展开更多
The feeding of children during weaning must be of good quality, this is essential, and it must not be a source of contamination to ensure the well-being of children. The objective of this work was to determine the bac...The feeding of children during weaning must be of good quality, this is essential, and it must not be a source of contamination to ensure the well-being of children. The objective of this work was to determine the bacteriological quality of the porridges and purees of weaning consumed by children from 6 to 24 months at the INSE of Guinea. For this, a descriptive study of analytical type was carried out over a period of 4 months at the INSE of Guinea. This study found that total mesophilic flora, faecal streptococci, staphylococci, and salmonella were consistent with sanitary standards in Plantin-enriched peanut paste and groundnut paste samples. They had respective bacteriological qualities of 90% and 60%. Fecal coliform contamination and ASR were observed respectively in the samples of rice husk remnants enriched with peanut paste and planting banana purée with respective values 50% and 40%. From the point of view of tampering, the samples of rice ground meal enriched with groundnut paste and Plantin banana purees were severely affected by the total coliforms at 40% and 60% respectively. In the end, poor hygiene practice during preparation increases the risk of food contamination;however, hygienic measures must be taken to improve the hygienic quality of food to maintain good health of children.展开更多
Objectives To investigate pre-and post-total middle ear reconstruction bacteriological changes in order to facilitate prevention and treatment of surgical infections. Methods Specimens from 128 ears in 124 patients wi...Objectives To investigate pre-and post-total middle ear reconstruction bacteriological changes in order to facilitate prevention and treatment of surgical infections. Methods Specimens from 128 ears in 124 patients with problematic mastoid cavities who underwent revision total middle ear reconstruction were studied. Results Bacteriological tests were positive in 87(68.0%) pre-operative specimens, despite local treatment, and in 74 (57.8%) specimens collected 10 days after operation. Common organism strains in pre-and post-operative specimens were slightly different and included: coagulase-staphylococcus, GRAM+ rods, staphylococcus aureus, pseudomonas aeruginosa and fungi. At the time of patient discharge(15-20 days post-operative), only 3 ears (2.3%) were tested positive which responded well to and became dry after local treatment. At one year, 4 ears (3.1%) showed recurrent otorrhea which again responded to local treatment. Conclusion For infection of problematic mastoid cavities after an open cavity procedure, revision total middle ear reconstruction is effective in controlling infection and promoting a dry ear. Infection prevention and treatment is important considering the relatively high rates of peri-operative local infections.展开更多
AIM:To report the microbiological spectrum of acute and chronic dacrocystitis.METHODS:Retrospective study on 100 patients who presented to the ophthalmic plastic clinic of a tertiary eye care center from May 2011 and ...AIM:To report the microbiological spectrum of acute and chronic dacrocystitis.METHODS:Retrospective study on 100 patients who presented to the ophthalmic plastic clinic of a tertiary eye care center from May 2011 and April 2013 with acute and chronic dacryocystitis was reviewed for demographic and microbiological profile. The culture results and organisms isolated were recorded.RESULTS:Sixty patients had acute onset and the remaining 40 patients had chronic onset dacryocystitis.The female to male ratio was 1.78. The mean age of patients was 44 y. Gram-positive organisms were the most commonly isolated accounting for 54%, and the commonest species isolated was S. aureus in 26%.Percentage of gram positive cultures was higher in chronic dacryocystitis than acute ones(82% vs 48% of positive cultures; P =0.003). Also in culture positive acute dacryocystitis, gram negative species were found in 52%of eyes but only in 18% of chronic dacryocystitis.CONCLUSION:Gram negative bacteria, culture negative samples, unusual and more virulent organisms are more common in acute dacryocystitis than chronic ones. The results of this study have significant bearing on the treatment of patients with dacrocystitis.展开更多
Aim： To report the detection in vitro of secretory inhibitor of platelet microbicidal protein （SIPMP） phenotypes of urethral isolates along with a comparisonwith isolates from patients with or without chronic bacte...Aim： To report the detection in vitro of secretory inhibitor of platelet microbicidal protein （SIPMP） phenotypes of urethral isolates along with a comparisonwith isolates from patients with or without chronic bacterial prostatitis （CBP）. Methods： Urethral isolates of Staphylococcus spp. （n = 64）, diphtheroids （n = 28）, micrococci （n = 15）, streptococci （n = 21）, Enterobacteriaceae （n = 9） and Enterococcusfaecalis （n = 19） from patients with or without CBP were tested. SIPMP production was tested by inhibition of platelet microbicidal protein （PMP） bioactivity against Bacillus subtilis and was expressed as percentage of inhibition of PMP bactericidal activity. Results： A significantly higher proportion of CBP-strains （57.78% vs. 16.67%） reduced PMP-induced killing of Bacillus subtilis than non- CBP strains did （P 〈 0.01）. SIPMP levels of staphylococci and Enterococcusfaecalis from the CBP group were significantly higher than those of the control group. Conclusion： These results suggest that SIPMP production is associated with the CBP source. Data from the present study might have significant implications for the understanding of the pathogenesis of CBP. （Asian J Androl 2008 Mar; 10： 189-192）.展开更多
Objective： To study the bacteriological profile in a healing mastoid cavity. Methods： This study was a single centre prospective study. Culture swabs from granulations in the mastoid cavity were sent in 40 consecuti...Objective： To study the bacteriological profile in a healing mastoid cavity. Methods： This study was a single centre prospective study. Culture swabs from granulations in the mastoid cavity were sent in 40 consecutive patients with squamosal chronic otitis media undergoing mastoidectomy. Cultures were processed for both aerobic and anaerobic bacteria. Results： Preoperatively： specimens from 26 out of 40 （60.5%） had growth on culture, with 22 （55%） showing only one organism while 4 showing multiple organisms. The commonest organism isolated was pseudomonas aeruginosa （n=15）. At 1 month after mastoidctomy, 11 patients had sterile culture while 29 had growth, of which 26 had aerobic growth and 3 had anaerobic growth. Pseudomonas was seen in 22 patients and staphylococcus aureus in 2 patients. The mean Merchant score was 2. At 3 months： 29 patients （72.5%） had sterile culture from mastoid cavity while 11 patients （27.5%） had growth on culture. All positive cultures were aerobic, including pseudomonas （n=9） and proteus （n= 2）. The mean Merchant score was 1.03. Of the 40 patients, 16 （40%） had a different organism cultured postoperatively compared to preoperative swabs. Conclusion： Pseudomonas and proteus seem to be the most common organisms responsible for persistent otorrhea after mastoidectomy. Persistent sterile otorrhea was seen in 4 patients （10%） in this group at the end of 3 months. Sterile cultures of preoperative swab are more likely to remain sterile in the postoperative period.展开更多
Brucellosis is an urgent infectious disease of livestock and wild animals and the commonest human zoonosis. Diagnosis of brucellosis is rather complicated and it has to be obligatorily confirmed by laboratory testing....Brucellosis is an urgent infectious disease of livestock and wild animals and the commonest human zoonosis. Diagnosis of brucellosis is rather complicated and it has to be obligatorily confirmed by laboratory testing. Direct bacteriological and molecular methods and indirect serological tests are used for brucellosis diagnostics. The choice of the diagnostic tools depends on the overall epidemiological situation in the region and the objectives of the study: validation of the diagnosis, screening (monitoring), cross-sectional studies or confirmation of brucellosis-free status of the region. The review describes current bacteriological, serological and molecular methods, routinely used for the diagnosis of brucellosis in humans and animals. The perspectives of brucellosis diagnostics are also discussed.展开更多
The Gounti Yéna valley, the subject of this study, is a tributary of the Niger River, it is the main watercourse that divides the left bank of the city of Niamey into two unequal parts. The surface area of its wa...The Gounti Yéna valley, the subject of this study, is a tributary of the Niger River, it is the main watercourse that divides the left bank of the city of Niamey into two unequal parts. The surface area of its watershed is estimated at about 62 Km2. The objective of the present study is on the one hand to evaluate the current state of the physico-chemical and bacteriological quality of the water of Gounti Yéna basin, and on the other hand to define the risks of water pollution and its origin in the face of the phenomenon of rising water table of this basin. In order to carry out this work, we proceeded to a study of the evolution of the physicochemical and bacteriological parameters of the water of the basin of Gounti Yéna during the period going from November 2020 to October 2021, at the level of four points of sampling chosen from the upstream to the downstream of the basin. The results obtained showed that the physico-chemical quality of the water in Gounti Yéna basin is influenced by the lithology of the watershed, climatic factors (rainfall and temperature) and anthropic actions. This study also highlighted the presence of fecal contamination in the water of the Gounti Yéna basin, with a greater degree of emphasis during the rainy season. This contamination remains a concern and may constitute a health risk.展开更多
The present study assesses the physicochemical and bacteriological quality of the drinking water used by the population of S?-Ava based on the Beninese standards and those established by the World Health Organization ...The present study assesses the physicochemical and bacteriological quality of the drinking water used by the population of S?-Ava based on the Beninese standards and those established by the World Health Organization (WHO). In rural and peri-urban areas of Benin where public water supply systems are inadequate or almost non-existent, the population consumes water of various sources of unknown qualities. A total of 67 water samples were analyzed during the rainy season (July 2017) and in the dry season (January 2018) for certain physical, chemical and bacteriological parameters using the standard methods. The results of the analyses reveal that the physicochemical characteristics of the water used for consumption in S?-Ava comply with the drinking water standards of the World Health Organization and those in force in Benin except for the percentages of the following parameters: pH (41.80%);turbidity (25.37%);the color (16.42);ammonium (17.91%);iron (40.30%);Nitrites (4.48%);Residual chlorine (91.05). Bacteriologically, the analyses showed a high total aerobic mesophilic flora contamination, faecal coliforms, E. coli, faecal enterococci respectively in 89.55%, 82.09%, 50.75% and 70.15% of the analyzed water samples. The ratio of faecal coliforms to faecal enterococci indicated that the origin of faecal contamination was human in 59.7% of the samples and animal in 40.3% of the samples. The adoption of hygiene measures at the water point, during the transport and storage of water, including the treatment by chlorination of drinking water at the family level was recommended for the population concerned and household awareness on the adoption of basic hygiene and sanitation measures have been recommended for hygiene and sanitation services.展开更多
Objective:To investigate clinicopathological,bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis(Y.pseudotuberculosis)in hares in order to verify the efficacy of sero...Objective:To investigate clinicopathological,bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis(Y.pseudotuberculosis)in hares in order to verify the efficacy of serology for the in vivo diagnosis.Moreover,the pathogenicity of two Y.pseudotuberculosis strains was investigated in order to detect potential differences.Methods:Twelve European brown hares(Lepus europaeus,Pallas)were experimentally infected per os and via conjunctival mucosae with Y.pseudotuberculosis:six subjects were infected with a strain isolated from a naturally infected hare(YpH)and six subjects with a strain isolated from a naturally infected rabbit(YpR).Two hares were used as negative controls.All animals were subjected to clinical,bacteriological and serological examinations during 9 weeks following the infection and,at the end of the control period,subjects still alive were euthanized and submitted to a complete post mortem examination.Results:All faecal samples collected during the control period were positive for bacteriological examinations and to a PCR for the inv gene of Y.pseudotuberculosis,while only one Yp H-infected hare showed a positive haemocultures.From the 2nd to the 9th week post infection(pi),serological analysis revealed specific antibodies with titers ranging from 1:10 to 1:160 in all YpH-infected and two YpR-infected subjects.All the Yp H-infected and two Yp R-infected hares scored positive for Y.pseudotuberculosis by means of bacteriological investigations.Grossly,suppurative multifocal lesions were detected in liver,spleen,kidney and sub-mandibular lymph nodes in both YpH-and YpR-infected hares and confirmed with histopathology.Pulmonary lesions were observed only in Yp H-infected subjects.Immunohistochemistry confirmed the presence of bacterial antigen in all infected animals.Conclusion:Results of this study revealed that YpH strain is more pathogenic for hares than the YpR strain;moreover the serological test performed in this study could be used for the diagnosis of pseudotuberculosis in hares,whereas post mortem diagnosis should be confirmed by means of bacteriological examination,PCR,histopathology and immunohistochemistry.展开更多
To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in eart...To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in earthen ponds maintained according to four management regimes： （1） live zooplankton fed to carp larvae （LF）; （2） direct fertilization with poultry manure （PM）; （3） direct fertilization with cowdung （CD）; and （4） a control treatment （C）. There were three replicates for each treatment. The growth of heterotrophic bacteria and pathogenic microorganisms like Aeromonas sp. and Pseudomonas sp. were also examined in response to pond management. Values of dissolved oxygen were significantly higher （P〈0.05） in the water of LF ponds, compared to other treatments, while the PM and CD treatments recorded were significantly higher （P〈0.05） values of PO4 - P, NH4 - N, NO3 - N, NO2 - N, specific conductivity, alkalinity, and BOD, compared to the LF and C treatments. The percentages of organic carbon and total nitrogen in the bottom sediments were higher in the PM and CD treatments compared to LF （P〈0.05）. Average counts of heterotrophic bacteria in the water of PM and CD ponds were significantly higher than other treatments （P〈0.05）. The development of Aeromonas sp. and Pseudomonas sp. were significantly higher （P〈0.05） in the PM and CD treatments. Weight gain of koi carp stocked in LF was significantly higher （P〈0.05） than that of fish in the other treatments. There was a significant difference in the survival rate of koi carp among the treatments ranging from 67.21% in C to 90.11% in LF. The results suggest that raising koi carp larvae in ponds and feeding them exogenously with zooplankton would support high rates of survival and production through maintenance of better water quality and greater abundance of zooplankton in the system. Significantly lower abundance of Aeromonas sp. and Pseudomonas sp. in the LF treatment considerably lowered any possibility of occurrence of bacterial disease.展开更多
Safe drinking water access for rural populations in developing countries remains a challenge for a sustainable development, particularly in rural and periurban areas of Burkina Faso. The study aims to investigate the ...Safe drinking water access for rural populations in developing countries remains a challenge for a sustainable development, particularly in rural and periurban areas of Burkina Faso. The study aims to investigate the purifying capacity of Moringa oleifera defatted cake as compared to Moringa oleifera seed in the treatment of surface and well waters used for populations alimentation. A total of 90 water samples were collected in sterile glass bottles from 3 dams’ water reservoirs, a river, and a large diameter well, respectively. The water samples were treated in triplicate with Moringa oleifera seed and defatted cake coagulants. At different settling time and coagulant concentration, turbidity and pH were measured to determine the optimal conditions and factors influencing treatment with regard to sampling source. Nine physicochemical parameters (turbidity, pH, nitrates, nitrites, calcium, magnesium, total hardness, organic matter and sulfates), three bacterial fecal pollution indicators (Escherichia coli, fecal Coliforms and fecal Streptococcus) and parasite cysts were monitored based on laboratory standard methods. Data were analyzed using the Student’s t test and XLSTAT 7.5.2 statistical software. From the results obtained, for the same concentration of coagulant, settling time providing the lowest turbidity was significantly shorter (p Moringa oleifera cake than seed. Optimum settling time with Moringa oleifera cake was between 15 - 60 min versus 60 - 120 min, with Moringa oleifera seed. Both treatments significantly reduced minerals concentration in water excepted sulfates for which the concentration reversely increased. However, only Moringa oleifera cake treatment reduced organic matter content in all the water samples, while it increased with Moringa oleifera seed one (p < 0.0001). The reduction of microbial pollution indicators was 92% - 100% with M. oleifera cake treatment and 84% - 100% with M. oleifera seed one. Overall, for all water samples, Moringa oleifera cake treatment appeared more efficient in improving drinking water quality than the M. oleifera seed treatment.展开更多
Background: Knowledge of the common bacteria that cause surgical site infection (SSI) and their antibiotic sensitivity is mandatory if treatment of surgical infection is to be successful. The threat of the emergence o...Background: Knowledge of the common bacteria that cause surgical site infection (SSI) and their antibiotic sensitivity is mandatory if treatment of surgical infection is to be successful. The threat of the emergence of resistant strains of bacteria is ever-present. Hence, a sensitivity directed therapy is paramount for the successful eradication of organisms with minimal risk of development of antibiotic resistance. Aim: The aim is to identify the common bacteria that cause SSI in orthopaedic implant surgeries in our hospital. Method: This is a prospective longitudinal study that includes all orthopaedic surgeries involving the use of implants within one year. Patients that had major orthopaedic surgeries involving implant were followed up and their wounds inspected for signs of SSI on postoperative days 3, 7, 14, 42 and 90. Wound swab was taken for microscopy, culture and sensitivity analysis from those who had wound infection, based on the CDC guidelines. Results: One-hundred and sixteen patients met our inclusion criteria and were included in the analysis. There were 62 males and 54 females. The mean age of the participant was 39.62 years (SD = 15.02 years). Fracture fixation with plates and screws was the most common implant surgery done. The incidence of SSI was 2.6%, and Escherichia coli was the most common isolated pathogen. All the SSIs were superficial incisional type, and the infection was monomicrobial in 67% of cases and polymicrobial in 33%. All of the isolated pathogens were sensitive to Imipenem and Gentamycin. Conclusion: Superficial incisional SSI is the most common type of SSI in this study. Escherichia coli is the most frequent pathogen in SSI affecting implant surgeries in our hospital. Gentamycin and Imipenem should be used for the prophylaxis of SSI in our environment.展开更多
文摘Background: Antibiotics have been used routinely for postoperative care in patients with chronic rhinosinusitis (CRS). However, increased bacterial growth was found after antibiotic treatment. In traditional Chinese medicine, Jia Wei Cang Er San has been used to treat CRS. This study was to investigate the influence of Jia Wei Cang Er San on the postoperative bacteriology of CRS. Methods: Ninety-seven CRS patients who underwent functional endoscopic sinus surgery (FESS) were included. They were randomly divided into 3 groups. In the group of Chinese herbal medicine (CHM), patients were given a capsule of Jia Wei Cang Er San tid for 8 weeks and a placebo capsule for amoxicillin q8h for 4 weeks after FESS. In the amoxicillin group, patients were given a capsule of amoxicillin 250 mg q8h for 4 weeks and a placebo capsule for Jia Wei Cang Er San tid for 8 weeks. In the placebo group, patients were given both placebo capsules. Bacterial cultures were performed from bilateral middle meati before FESS, and 8 and 12 weeks after FESS. Results: In the CHM group, bacteria grew in 21 (46%) of 46 specimens pre-operatively, in 23 (50%) specimens 8 weeks and in 17 (37%) specimens 12 weeks after surgery. In the amoxicillin group, bacteria grew in 15 (28%) of 54 specimens pre-operatively, in 30 (56%) specimens 8 weeks and in 32 (59%) specimens 12 weeks after surgery. In the placebo group, bacteria were found in 13 (34%) of 38 specimens pre-operatively, in 16 (42%) specimens 8 weeks and in 12 (32%) specimens 12 weeks after surgery. The rates of bacterial growth did not change by Jia Wei Cang Er San 8 or 12 weeks after surgery, but increased significantly by amoxicillin 8 and 12 weeks after surgery. Conclusion: Our study showed that Jia Wei Cang Er San did not induce bacterial growth after FESS as amoxicillin.
基金Supported by Project on Education and Teaching Reform of Higher Education Institutions in Guangdong ProvinceApplied Personnel Training Course of Guangdong Ocean University+1 种基金Industry-University-Research Institute Cooperation Collaborative Education Teaching Content and Curriculum System Reform Project of Ministry of Education(202101134003)Project of Degree and Postgraduate Education and Teaching Reform of Guangdong Ocean University(202020&202120).
文摘Aquatic Animal Bacteriology is a basic course of Aquatic Animal Medicine discipline.Bacteriology is not only a frontier subject in the field of life sciences,but also one of the most rapidly developing scientific fields today.The Belt and Road Initiative has brought opportunities and challenges to the upgrading and development of this discipline,so this discipline faces many problems that need to be solved.In view of this,this study combined the development trend of the discipline,reorganized and optimized the course content,and prepared sophisticated multimedia courseware,to stimulate students'learning initiative and enthusiasm,expand the field of professional knowledge,improve their English translation and writing skills,so as to lay a solid foundation for their future service to the Belt and Road Initiative.
文摘Bacteria are smallest primitive, simple, unicellular, prokaryotic and microscopic organisms. But these organisms cannot be studied with naked eyes because of their minute structure. Therefore in search for the information about the structure and composition of bacterial cells, cell biologist used light microscopes with a numerical aperture of 1.4 and using wavelength of 0.4 μm separation. But there are still certain cellular structures that cannot be seen through naked eyes, and for them electron microscope is used. There are certain improved types of light microscope which can be incorporated to improve their resolving power. Hence microscopy is playing a crucial role in the field of bacteriology.
基金financed by the grant from the Philosophy and Social Sciences Research Project for Colleges and Universities in Jiangsu Province(No.2022SJYB0321).
文摘The paper examines the response of Chinese medicine and the general public to the introduction of the first-ever synthetic chemical agent of Salvarsan(洒尔佛散)to the Chinese market.Since the new medicine was first introduced in 1910,the feedback has been predominantly positive.The rapid efficacy of Salvarsan was also recognised by Chinese medicine practitioners,who integrated it into the Chinese traditional herbal classification system and redefined its pharmacological properties in Chinese medicine theories.Nevertheless,Chinese medicine’s perspective on Salvarsan goes beyond mere acceptance.The discourse surrounding Salvarsan encompasses its therapeutic effectiveness and delves deeper into broader themes concerning the fundamental principles of the medication as well as the dispute between Chinese and Western medicine that transcends medical theory.To maintain the validity of Chinese medicine theory in the discussion that went beyond simple medical theory,Chinese medicine used Salvarsan as evidence against the notion that bacteriological theory was the sole reliable theory of disease.
基金funding from the European Union’s Horizon 2020 Research&Innovation Programme(2211)under the Partnership for Research and Innovation in the Mediterranean Area(PRIMA)Project"SHARInG-MeD"from the Directorate-General for Scientific Research and Technological Development(DGRSDT)under the Projets de Recherche Formation-Universitaire(PRFU)Projects(D00L02UN120120230002,D01N01UN120120230005)。
文摘Groundwater quality assessment is important to assure safe and durable water use.In semi-arid areas of Algeria,groundwater represents the main water resource for drinking water supply of the rural population as well as for irrigation of agricultural lands.Groundwater samples from wells and springs were collected from the Gargaat Tarf and Annk Djemel sub-watersheds of the Oum El Bouaghi,Algeria,and were analyzed and compared with the World Health Organization(WHO)standards.Results showed that most of the measured physical and chemical parameters exceeded the quality limits according to the WHO standards.Groundwater had a slightly alkaline water pH(7.00-7.79),electrical conductivity>1500μS/cm,chloride>500 mg/L,calcium>250 mg/L,and magnesium>155 mg/L.Water quality index(WQI)results showed that 68%of the area had excellent water quality,24%of the samples fell into good category,and only 8%were of poor quality and unsuitable for human consumption.Six wells in the area showed bacterial contamination.Total coliforms(453.9(±180.3)CFU(colony-forming units)/100 mL),fecal coliforms(243.2(±99.2)CFU/100 mL),and fecal streptococci(77.9(±32.0)CFU/100 mL)loads were above the standard limits set by the WHO.These results confirmed that water resources in the study area were strongly influenced by anthropogenic activities and were not recommended for consumption as drinking water.
文摘Background: Despite a significant decline in neonatal deaths in the last 20 years (5 million in 1990 to 2.4 million in 2019), the risk of death is still high, especially in developing countries. In Tanzania, neonatal sepsis is the third leading cause of neonatal death, accounting for 25% of all deaths. The rising global threat of antimicrobial resistance and the rising burden of neonatal death due to neonatal sepsis have been of great concern and have delayed progress toward reaching SDG goal 3.2 by 2030. This study aims to determine the bacteriological profile, antibiotic susceptibility patterns, and predictors of bacteremia among neonates with clinical sepsis at KCMC Hospital in Northern Tanzania. Methodology: This study had a cross-sectional design conducted at KCMC Hospital, Northern Tanzania. The study population was neonates admitted to the neonatal unit at KCMC Hospital. Data were collected using questionnaires and blood cultures from neonates. Frequencies and proportions were used to summarize categorical variables, while continuous variables were summarized using mean and standard deviation. The frequencies and proportions of bacteria isolated and the antimicrobial susceptibility results were analyzed and compared using Pearson’s chi-square test and Fisher’s exact test where applicable. Modified Poisson regression model was used to determine factors associated with positive blood culture. Results: Out of 411 neonates with a clinical diagnosis of neonatal sepsis, 175 (42.9%) had positive blood cultures. Gram-positive bacteria were most frequently isolated at 52.3%, and gram-negative bacteria were 47.7%. Coagulase-negative Staphylococcus (30.7%) and Staphylococcus aureus (19.9%) were the predominant gram-positive isolates. Gram-negative isolates were Klebsiella spp 47 (26.7%), E. coli 10 (5.7%), and Citrobacter spp 10 (5.1%). The gram-positive isolates were sensitive to vancomycin, piperacillin/tazobactam, and ceftazidime, whereas the gram-negative were sensitive to amikacin, meropenem, and vancomycin. The study did not find statistically significant associations between clinical factors and positive blood cultures in bacteremia. Conclusion: Gram-positive bacteria are the dominant pathogens in early-onset and late-onset neonatal sepsis. High levels of resistance to ampicillin and ceftriaxone and moderate resistance to gentamycin were observed in both gram-positive and gram-negative bacteria. Gram-positive organisms exhibit better susceptibility rates to vancomycin and ciprofloxacin, while gram-negative micro-organisms are more sensitive to amikacin and meropenem. An effective initial treatment approach for neonatal sepsis would involve a combination of drugs.
文摘Introduction: Microbiology of effusion fluids in children in Burkina Faso is characterized by the scarcity of data. This work aimed to study the bacteriological and antibiotics susceptibility profile of bacteria involved in effusion fluid infections in paediatrics in order to improve the choice of probabilistic antibiotics therapy. Methods: A cross-sectional, descriptive study was used in children aged 0 to 15 years from 2017 to 2020 at the Charles De Gaulle Pediatric University Hospital Center (CHUP-CDG) in Ouagadougou. Classical bacteriology methods such as macroscopy, Gram staining, identification galleries and antibiotics susceptibility testing were used. Results: Of 231 samples, 64 bacteria were isolated. The most common bacterial strains of pleural fluid were Staphylococcus aureus (25%) and 40% for Enterobacteriaceae. Of the peritoneal fluid, 77% were Enterobacteriaceae with 57% Escherichia coli;and from joint fluid, 33% were S. aureus and 22% for P. aeruginosa. The overall susceptibility profile showed 29% extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL), 10% methicillin-resistant S. aureus (MRSA), and 8% carbapenemases. Conclusion: Bacteriological profile is characterized by ESBL-producing Enterobacteriaceae and MRSA. The most active antibiotics were macrolides, aminoglycosides, and cefoxitin (methicillin) for Gram-positive cocci, carbapenems, and aminoglycosides for Gram-negative bacilli. Then, the monitoring of antibiotics resistance must be permanent.
文摘The feeding of children during weaning must be of good quality, this is essential, and it must not be a source of contamination to ensure the well-being of children. The objective of this work was to determine the bacteriological quality of the porridges and purees of weaning consumed by children from 6 to 24 months at the INSE of Guinea. For this, a descriptive study of analytical type was carried out over a period of 4 months at the INSE of Guinea. This study found that total mesophilic flora, faecal streptococci, staphylococci, and salmonella were consistent with sanitary standards in Plantin-enriched peanut paste and groundnut paste samples. They had respective bacteriological qualities of 90% and 60%. Fecal coliform contamination and ASR were observed respectively in the samples of rice husk remnants enriched with peanut paste and planting banana purée with respective values 50% and 40%. From the point of view of tampering, the samples of rice ground meal enriched with groundnut paste and Plantin banana purees were severely affected by the total coliforms at 40% and 60% respectively. In the end, poor hygiene practice during preparation increases the risk of food contamination;however, hygienic measures must be taken to improve the hygienic quality of food to maintain good health of children.
文摘Objectives To investigate pre-and post-total middle ear reconstruction bacteriological changes in order to facilitate prevention and treatment of surgical infections. Methods Specimens from 128 ears in 124 patients with problematic mastoid cavities who underwent revision total middle ear reconstruction were studied. Results Bacteriological tests were positive in 87(68.0%) pre-operative specimens, despite local treatment, and in 74 (57.8%) specimens collected 10 days after operation. Common organism strains in pre-and post-operative specimens were slightly different and included: coagulase-staphylococcus, GRAM+ rods, staphylococcus aureus, pseudomonas aeruginosa and fungi. At the time of patient discharge(15-20 days post-operative), only 3 ears (2.3%) were tested positive which responded well to and became dry after local treatment. At one year, 4 ears (3.1%) showed recurrent otorrhea which again responded to local treatment. Conclusion For infection of problematic mastoid cavities after an open cavity procedure, revision total middle ear reconstruction is effective in controlling infection and promoting a dry ear. Infection prevention and treatment is important considering the relatively high rates of peri-operative local infections.
文摘AIM:To report the microbiological spectrum of acute and chronic dacrocystitis.METHODS:Retrospective study on 100 patients who presented to the ophthalmic plastic clinic of a tertiary eye care center from May 2011 and April 2013 with acute and chronic dacryocystitis was reviewed for demographic and microbiological profile. The culture results and organisms isolated were recorded.RESULTS:Sixty patients had acute onset and the remaining 40 patients had chronic onset dacryocystitis.The female to male ratio was 1.78. The mean age of patients was 44 y. Gram-positive organisms were the most commonly isolated accounting for 54%, and the commonest species isolated was S. aureus in 26%.Percentage of gram positive cultures was higher in chronic dacryocystitis than acute ones(82% vs 48% of positive cultures; P =0.003). Also in culture positive acute dacryocystitis, gram negative species were found in 52%of eyes but only in 18% of chronic dacryocystitis.CONCLUSION:Gram negative bacteria, culture negative samples, unusual and more virulent organisms are more common in acute dacryocystitis than chronic ones. The results of this study have significant bearing on the treatment of patients with dacrocystitis.
文摘Aim： To report the detection in vitro of secretory inhibitor of platelet microbicidal protein （SIPMP） phenotypes of urethral isolates along with a comparisonwith isolates from patients with or without chronic bacterial prostatitis （CBP）. Methods： Urethral isolates of Staphylococcus spp. （n = 64）, diphtheroids （n = 28）, micrococci （n = 15）, streptococci （n = 21）, Enterobacteriaceae （n = 9） and Enterococcusfaecalis （n = 19） from patients with or without CBP were tested. SIPMP production was tested by inhibition of platelet microbicidal protein （PMP） bioactivity against Bacillus subtilis and was expressed as percentage of inhibition of PMP bactericidal activity. Results： A significantly higher proportion of CBP-strains （57.78% vs. 16.67%） reduced PMP-induced killing of Bacillus subtilis than non- CBP strains did （P 〈 0.01）. SIPMP levels of staphylococci and Enterococcusfaecalis from the CBP group were significantly higher than those of the control group. Conclusion： These results suggest that SIPMP production is associated with the CBP source. Data from the present study might have significant implications for the understanding of the pathogenesis of CBP. （Asian J Androl 2008 Mar; 10： 189-192）.
文摘Objective： To study the bacteriological profile in a healing mastoid cavity. Methods： This study was a single centre prospective study. Culture swabs from granulations in the mastoid cavity were sent in 40 consecutive patients with squamosal chronic otitis media undergoing mastoidectomy. Cultures were processed for both aerobic and anaerobic bacteria. Results： Preoperatively： specimens from 26 out of 40 （60.5%） had growth on culture, with 22 （55%） showing only one organism while 4 showing multiple organisms. The commonest organism isolated was pseudomonas aeruginosa （n=15）. At 1 month after mastoidctomy, 11 patients had sterile culture while 29 had growth, of which 26 had aerobic growth and 3 had anaerobic growth. Pseudomonas was seen in 22 patients and staphylococcus aureus in 2 patients. The mean Merchant score was 2. At 3 months： 29 patients （72.5%） had sterile culture from mastoid cavity while 11 patients （27.5%） had growth on culture. All positive cultures were aerobic, including pseudomonas （n=9） and proteus （n= 2）. The mean Merchant score was 1.03. Of the 40 patients, 16 （40%） had a different organism cultured postoperatively compared to preoperative swabs. Conclusion： Pseudomonas and proteus seem to be the most common organisms responsible for persistent otorrhea after mastoidectomy. Persistent sterile otorrhea was seen in 4 patients （10%） in this group at the end of 3 months. Sterile cultures of preoperative swab are more likely to remain sterile in the postoperative period.
文摘Brucellosis is an urgent infectious disease of livestock and wild animals and the commonest human zoonosis. Diagnosis of brucellosis is rather complicated and it has to be obligatorily confirmed by laboratory testing. Direct bacteriological and molecular methods and indirect serological tests are used for brucellosis diagnostics. The choice of the diagnostic tools depends on the overall epidemiological situation in the region and the objectives of the study: validation of the diagnosis, screening (monitoring), cross-sectional studies or confirmation of brucellosis-free status of the region. The review describes current bacteriological, serological and molecular methods, routinely used for the diagnosis of brucellosis in humans and animals. The perspectives of brucellosis diagnostics are also discussed.
文摘The Gounti Yéna valley, the subject of this study, is a tributary of the Niger River, it is the main watercourse that divides the left bank of the city of Niamey into two unequal parts. The surface area of its watershed is estimated at about 62 Km2. The objective of the present study is on the one hand to evaluate the current state of the physico-chemical and bacteriological quality of the water of Gounti Yéna basin, and on the other hand to define the risks of water pollution and its origin in the face of the phenomenon of rising water table of this basin. In order to carry out this work, we proceeded to a study of the evolution of the physicochemical and bacteriological parameters of the water of the basin of Gounti Yéna during the period going from November 2020 to October 2021, at the level of four points of sampling chosen from the upstream to the downstream of the basin. The results obtained showed that the physico-chemical quality of the water in Gounti Yéna basin is influenced by the lithology of the watershed, climatic factors (rainfall and temperature) and anthropic actions. This study also highlighted the presence of fecal contamination in the water of the Gounti Yéna basin, with a greater degree of emphasis during the rainy season. This contamination remains a concern and may constitute a health risk.
基金the International Foundation for Science(IFS)for contributing to this work.
文摘The present study assesses the physicochemical and bacteriological quality of the drinking water used by the population of S?-Ava based on the Beninese standards and those established by the World Health Organization (WHO). In rural and peri-urban areas of Benin where public water supply systems are inadequate or almost non-existent, the population consumes water of various sources of unknown qualities. A total of 67 water samples were analyzed during the rainy season (July 2017) and in the dry season (January 2018) for certain physical, chemical and bacteriological parameters using the standard methods. The results of the analyses reveal that the physicochemical characteristics of the water used for consumption in S?-Ava comply with the drinking water standards of the World Health Organization and those in force in Benin except for the percentages of the following parameters: pH (41.80%);turbidity (25.37%);the color (16.42);ammonium (17.91%);iron (40.30%);Nitrites (4.48%);Residual chlorine (91.05). Bacteriologically, the analyses showed a high total aerobic mesophilic flora contamination, faecal coliforms, E. coli, faecal enterococci respectively in 89.55%, 82.09%, 50.75% and 70.15% of the analyzed water samples. The ratio of faecal coliforms to faecal enterococci indicated that the origin of faecal contamination was human in 59.7% of the samples and animal in 40.3% of the samples. The adoption of hygiene measures at the water point, during the transport and storage of water, including the treatment by chlorination of drinking water at the family level was recommended for the population concerned and household awareness on the adoption of basic hygiene and sanitation measures have been recommended for hygiene and sanitation services.
文摘Objective:To investigate clinicopathological,bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis(Y.pseudotuberculosis)in hares in order to verify the efficacy of serology for the in vivo diagnosis.Moreover,the pathogenicity of two Y.pseudotuberculosis strains was investigated in order to detect potential differences.Methods:Twelve European brown hares(Lepus europaeus,Pallas)were experimentally infected per os and via conjunctival mucosae with Y.pseudotuberculosis:six subjects were infected with a strain isolated from a naturally infected hare(YpH)and six subjects with a strain isolated from a naturally infected rabbit(YpR).Two hares were used as negative controls.All animals were subjected to clinical,bacteriological and serological examinations during 9 weeks following the infection and,at the end of the control period,subjects still alive were euthanized and submitted to a complete post mortem examination.Results:All faecal samples collected during the control period were positive for bacteriological examinations and to a PCR for the inv gene of Y.pseudotuberculosis,while only one Yp H-infected hare showed a positive haemocultures.From the 2nd to the 9th week post infection(pi),serological analysis revealed specific antibodies with titers ranging from 1:10 to 1:160 in all YpH-infected and two YpR-infected subjects.All the Yp H-infected and two Yp R-infected hares scored positive for Y.pseudotuberculosis by means of bacteriological investigations.Grossly,suppurative multifocal lesions were detected in liver,spleen,kidney and sub-mandibular lymph nodes in both YpH-and YpR-infected hares and confirmed with histopathology.Pulmonary lesions were observed only in Yp H-infected subjects.Immunohistochemistry confirmed the presence of bacterial antigen in all infected animals.Conclusion:Results of this study revealed that YpH strain is more pathogenic for hares than the YpR strain;moreover the serological test performed in this study could be used for the diagnosis of pseudotuberculosis in hares,whereas post mortem diagnosis should be confirmed by means of bacteriological examination,PCR,histopathology and immunohistochemistry.
文摘To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in earthen ponds maintained according to four management regimes： （1） live zooplankton fed to carp larvae （LF）; （2） direct fertilization with poultry manure （PM）; （3） direct fertilization with cowdung （CD）; and （4） a control treatment （C）. There were three replicates for each treatment. The growth of heterotrophic bacteria and pathogenic microorganisms like Aeromonas sp. and Pseudomonas sp. were also examined in response to pond management. Values of dissolved oxygen were significantly higher （P〈0.05） in the water of LF ponds, compared to other treatments, while the PM and CD treatments recorded were significantly higher （P〈0.05） values of PO4 - P, NH4 - N, NO3 - N, NO2 - N, specific conductivity, alkalinity, and BOD, compared to the LF and C treatments. The percentages of organic carbon and total nitrogen in the bottom sediments were higher in the PM and CD treatments compared to LF （P〈0.05）. Average counts of heterotrophic bacteria in the water of PM and CD ponds were significantly higher than other treatments （P〈0.05）. The development of Aeromonas sp. and Pseudomonas sp. were significantly higher （P〈0.05） in the PM and CD treatments. Weight gain of koi carp stocked in LF was significantly higher （P〈0.05） than that of fish in the other treatments. There was a significant difference in the survival rate of koi carp among the treatments ranging from 67.21% in C to 90.11% in LF. The results suggest that raising koi carp larvae in ponds and feeding them exogenously with zooplankton would support high rates of survival and production through maintenance of better water quality and greater abundance of zooplankton in the system. Significantly lower abundance of Aeromonas sp. and Pseudomonas sp. in the LF treatment considerably lowered any possibility of occurrence of bacterial disease.
文摘Safe drinking water access for rural populations in developing countries remains a challenge for a sustainable development, particularly in rural and periurban areas of Burkina Faso. The study aims to investigate the purifying capacity of Moringa oleifera defatted cake as compared to Moringa oleifera seed in the treatment of surface and well waters used for populations alimentation. A total of 90 water samples were collected in sterile glass bottles from 3 dams’ water reservoirs, a river, and a large diameter well, respectively. The water samples were treated in triplicate with Moringa oleifera seed and defatted cake coagulants. At different settling time and coagulant concentration, turbidity and pH were measured to determine the optimal conditions and factors influencing treatment with regard to sampling source. Nine physicochemical parameters (turbidity, pH, nitrates, nitrites, calcium, magnesium, total hardness, organic matter and sulfates), three bacterial fecal pollution indicators (Escherichia coli, fecal Coliforms and fecal Streptococcus) and parasite cysts were monitored based on laboratory standard methods. Data were analyzed using the Student’s t test and XLSTAT 7.5.2 statistical software. From the results obtained, for the same concentration of coagulant, settling time providing the lowest turbidity was significantly shorter (p Moringa oleifera cake than seed. Optimum settling time with Moringa oleifera cake was between 15 - 60 min versus 60 - 120 min, with Moringa oleifera seed. Both treatments significantly reduced minerals concentration in water excepted sulfates for which the concentration reversely increased. However, only Moringa oleifera cake treatment reduced organic matter content in all the water samples, while it increased with Moringa oleifera seed one (p < 0.0001). The reduction of microbial pollution indicators was 92% - 100% with M. oleifera cake treatment and 84% - 100% with M. oleifera seed one. Overall, for all water samples, Moringa oleifera cake treatment appeared more efficient in improving drinking water quality than the M. oleifera seed treatment.
文摘Background: Knowledge of the common bacteria that cause surgical site infection (SSI) and their antibiotic sensitivity is mandatory if treatment of surgical infection is to be successful. The threat of the emergence of resistant strains of bacteria is ever-present. Hence, a sensitivity directed therapy is paramount for the successful eradication of organisms with minimal risk of development of antibiotic resistance. Aim: The aim is to identify the common bacteria that cause SSI in orthopaedic implant surgeries in our hospital. Method: This is a prospective longitudinal study that includes all orthopaedic surgeries involving the use of implants within one year. Patients that had major orthopaedic surgeries involving implant were followed up and their wounds inspected for signs of SSI on postoperative days 3, 7, 14, 42 and 90. Wound swab was taken for microscopy, culture and sensitivity analysis from those who had wound infection, based on the CDC guidelines. Results: One-hundred and sixteen patients met our inclusion criteria and were included in the analysis. There were 62 males and 54 females. The mean age of the participant was 39.62 years (SD = 15.02 years). Fracture fixation with plates and screws was the most common implant surgery done. The incidence of SSI was 2.6%, and Escherichia coli was the most common isolated pathogen. All the SSIs were superficial incisional type, and the infection was monomicrobial in 67% of cases and polymicrobial in 33%. All of the isolated pathogens were sensitive to Imipenem and Gentamycin. Conclusion: Superficial incisional SSI is the most common type of SSI in this study. Escherichia coli is the most frequent pathogen in SSI affecting implant surgeries in our hospital. Gentamycin and Imipenem should be used for the prophylaxis of SSI in our environment.
