目的分析肝细胞癌组织及配对癌旁组织的蛋白质组数据,筛选与肝细胞癌患者预后相关的生物标志物。方法通过对5组肝细胞癌组织及配对癌旁组织进行定量蛋白质组学测序,利用差异分析筛选出差异表达蛋白质。对差异表达蛋白质进行GeneOntology...目的分析肝细胞癌组织及配对癌旁组织的蛋白质组数据,筛选与肝细胞癌患者预后相关的生物标志物。方法通过对5组肝细胞癌组织及配对癌旁组织进行定量蛋白质组学测序,利用差异分析筛选出差异表达蛋白质。对差异表达蛋白质进行GeneOntology(GO)功能注释和Kyoto encyclopedia of genes and genomes(KEGG)通路分析。同时,利用公共数据库和组织芯片对预后相关的差异表达蛋白质进行筛选与验证。结果在肝细胞癌组织和癌旁组织间共筛选出差异表达蛋白质820个。相比癌旁组织,在肝细胞癌组织中上调的蛋白质有422个,下调的蛋白质有398个。GO功能注释和KEGG通路分析的结果表明差异表达蛋白质富集到细胞内重要的生物学过程、结构和信号通路。在mRNA和蛋白质水平上,BAG3在肝细胞癌组织中的表达水平显著高于正常肝组织,并且BAG3高表达与肝细胞癌患者较差的预后相关。BAG3高表达是肝细胞癌患者预后的独立危险因素。结论BAG3高表达与肝细胞癌患者预后不良相关,BAG3可作为肝细胞癌患者的预后标志物。展开更多
Multiple regression equations of liquidus temperature, electrical conductivity and bath density of the Na_3AlF_6-AlF_3-BaC1_2-NaCl system were obtained from experiments by using orthogonal regression method. The exper...Multiple regression equations of liquidus temperature, electrical conductivity and bath density of the Na_3AlF_6-AlF_3-BaC1_2-NaCl system were obtained from experiments by using orthogonal regression method. The experiments were carried out in 100A cell with low melting point electrolyte, the influences of cathodic current density, electrolytic temperature, density differences of bath and liquid aluminum on current efficiency (CE) were studied; when the electrolyte cryolite ratio was 2.5, w(BaC1_2) and w(NaCl) were 48% and 10%, respectively, CE reached 90% and specific energy consumption was 10.97k Wb/kg/kg. Because of the fact that aluminum metal obtained floated on the surface of molten electrolyte, this electrolysis method was then defined as low temperature aluminum floating electrolysis. The results showed that the new low temperature aluminum electrolysis process in the Na_3AlF_6-AlF_3-BaC1_2-NaCl bath system was practical and promising.展开更多
文摘目的分析肝细胞癌组织及配对癌旁组织的蛋白质组数据,筛选与肝细胞癌患者预后相关的生物标志物。方法通过对5组肝细胞癌组织及配对癌旁组织进行定量蛋白质组学测序,利用差异分析筛选出差异表达蛋白质。对差异表达蛋白质进行GeneOntology(GO)功能注释和Kyoto encyclopedia of genes and genomes(KEGG)通路分析。同时,利用公共数据库和组织芯片对预后相关的差异表达蛋白质进行筛选与验证。结果在肝细胞癌组织和癌旁组织间共筛选出差异表达蛋白质820个。相比癌旁组织,在肝细胞癌组织中上调的蛋白质有422个,下调的蛋白质有398个。GO功能注释和KEGG通路分析的结果表明差异表达蛋白质富集到细胞内重要的生物学过程、结构和信号通路。在mRNA和蛋白质水平上,BAG3在肝细胞癌组织中的表达水平显著高于正常肝组织,并且BAG3高表达与肝细胞癌患者较差的预后相关。BAG3高表达是肝细胞癌患者预后的独立危险因素。结论BAG3高表达与肝细胞癌患者预后不良相关,BAG3可作为肝细胞癌患者的预后标志物。
基金The project was financially supported by the National Natural Science Foundation of China! (Gmnt No.59574018)China Postdocto
文摘Multiple regression equations of liquidus temperature, electrical conductivity and bath density of the Na_3AlF_6-AlF_3-BaC1_2-NaCl system were obtained from experiments by using orthogonal regression method. The experiments were carried out in 100A cell with low melting point electrolyte, the influences of cathodic current density, electrolytic temperature, density differences of bath and liquid aluminum on current efficiency (CE) were studied; when the electrolyte cryolite ratio was 2.5, w(BaC1_2) and w(NaCl) were 48% and 10%, respectively, CE reached 90% and specific energy consumption was 10.97k Wb/kg/kg. Because of the fact that aluminum metal obtained floated on the surface of molten electrolyte, this electrolysis method was then defined as low temperature aluminum floating electrolysis. The results showed that the new low temperature aluminum electrolysis process in the Na_3AlF_6-AlF_3-BaC1_2-NaCl bath system was practical and promising.
