Reprogrammed metabolism is a hallmark of cancer. Glioblastoma（GBM） tumor cells predominantly utilize aerobic glycolysis for the biogenesis of energy and intermediate nutrients. However, in GBM, the clinical signific...Reprogrammed metabolism is a hallmark of cancer. Glioblastoma（GBM） tumor cells predominantly utilize aerobic glycolysis for the biogenesis of energy and intermediate nutrients. However, in GBM, the clinical significance of glycolysis and its underlying relations with the molecular features such as IDH1 mutation and subtype have not been elucidated yet. Herein, based on glioma datasets including TCGA（The Cancer Genome Atlas）, REMBRANDT（Repository for Molecular Brain Neoplasia Data） and GSE16011 we established a glycolytic gene expression signature score（GGESS） by incorporating ten glycolytic genes. Then we performed survival analyses and investigated the correlations between GGESS and IDH1 mutation as well as the molecular subtypes in GBMs. The results showed that GGESS independently predicted unfavorable prognosis and poor response to chemotherapy of GBM patients. Notably, GGESS was high in GBMs of mesenchymal subtype but low in IDH1-mutant GBMs. Furthermore, we found that the promoter regions of tumor-promoting glycolytic genes were hypermethylated in IDH1-mutant GBMs.Finally, we found that high GGESS also predicted poor prognosis and poor response to chemotherapy when investigating IDH1-wild type GBM patients only. Collectively, glycolysis represented by GGESS predicts unfavorable clinical outcome of GBM patients and is closely associated with mesenchymal subtype and IDH1 mutation in GBMs.展开更多
Background:SARS-CoV-2,first identified in late 2019,has given rise to numerous variants of concern(VOCs),posing a significant threat to human health.The emer-gence of Omicron BA.1.1 towards the end of 2021 led to a pa...Background:SARS-CoV-2,first identified in late 2019,has given rise to numerous variants of concern(VOCs),posing a significant threat to human health.The emer-gence of Omicron BA.1.1 towards the end of 2021 led to a pandemic in early 2022.At present,the lethal mouse model for the study of SARS-CoV-2 needs supplementation,and the alterations in neutrophils and monocytes caused by different strains remain to be elucidated.Methods:Human ACE2 transgenic mice were inoculated with the SARS-CoV-2 proto-type and Omicron BA.1,respectively.The pathogenicity of the two strains was evalu-ated by observing clinical symptoms,viral load and pathology.Complete blood count,immunohistochemistry and flow cytometry were performed to detect the alterations of neutrophils and monocytes caused by the two strains.Results:Our findings revealed that Omicron BA.1 exhibited significantly lower vir-ulence compared to the SARS-CoV-2 prototype in the mouse model.Additionally,we observed a significant increase in the proportion of neutrophils late in infection with the SARS-CoV-2 prototype and Omicron BA.1.We found that the proportion of monocytes increased at first and then decreased.The trends in the changes in the proportions of neutrophils and monocytes induced by the two strains were similar.Conclusion:Our study provides valuable insights into the utility of mouse models for simulating the severe disease of SARS-CoV-2 prototype infection and the milder manifestation associated with Omicron BA.1.SARS-CoV-2 prototype and Omicron BA.1 resulted in similar trends in the changes in neutrophils and monocytes.展开更多
Diarrhea predominant irritable bowel syndrome(IBS-D)have a serious impact on the patient’s quality life due to the lack of safe and effective drugs.The transient receptor potential vanilloid subtype 1(TRPV1)is an ion...Diarrhea predominant irritable bowel syndrome(IBS-D)have a serious impact on the patient’s quality life due to the lack of safe and effective drugs.The transient receptor potential vanilloid subtype 1(TRPV1)is an ion channel receptor implicated in the perception of visceral injury.Recent studies indicated that TRPV1 mediates visceral hypersensitivity in IBS-D patients by enhancing the excitability of intestinal sensory neurons.Consequently,inhibiting the TRPV1 may be a promising option for the treatment of IBS-D.Current research demonstrates that various traditional Chinese medicine(TCM)methods,such as herbal prescriptions,acupuncture,and moxibustion,can reduce visceral sensitivity by regulating TRPV1 expression and its activation sensitization.This suggests that TCM methods may serve as safe and effective options for alleviating IBS-D.Therefore,this article summarizes potential therapeutic strategies of TCM as a regulator of TRPV1 for managing IBS-D.It also provides insights into potential TCM methods and natural phytochemical molecular nuclei for future drug research targeting TRPV1 in IBS-D.展开更多
The HA1 gene of H3N2 subtype swine influenza virus(SIV)was cloned into the expression plasmid pET-30a,the recombinant plasmid was named pET-HAl.This was transformed into E.coli BL21(DE3),and expressed by induction wit...The HA1 gene of H3N2 subtype swine influenza virus(SIV)was cloned into the expression plasmid pET-30a,the recombinant plasmid was named pET-HAl.This was transformed into E.coli BL21(DE3),and expressed by induction with IPTG.The expressed HA protein was identified by SDS-PAGE and Western blotting which showed the protein to be 42kDa and was immunoreactive.The purified HA protein was used to establish the indirect ELIS A for detection of the antibodies,specifically against the H3 subtype of SIV.The assay has excellent specificity,sensitivity and reproducibility.When 96 serum samples,randomly collected from the field,were evaluated in parallel by this new ELISA using recombinant HA1 and a routine HI test,the coincidental rate between the two tests was 86.5%.These results show that the recombinant HAl-based ELISA is specific,sensitive and easy to perform for the serological diagnosis of SIV infection.展开更多
At room temperature, Ba1-xCaxMoO4 polycrystalline solid solution thin films were prepared directly in contained Ca2+ and Ba2+ alkaline solution by electrochemical anode oxidation method. The crystal phase structure, s...At room temperature, Ba1-xCaxMoO4 polycrystalline solid solution thin films were prepared directly in contained Ca2+ and Ba2+ alkaline solution by electrochemical anode oxidation method. The crystal phase structure, surface morphology, ratio of compositions and room temperature fluorescence properties of the as-grown films were measured by using XRD, SEM, EDAX and Fluorescence Analysis techniques, respectively. The results reveal that the ratio of composition of Ba1-xCaxMoO4 films can be modulated by controlling the ratio of Ca2+ and Ba2+ in starting solutions; moreover it has important influence of ratio of composition of the films on the grain growth, the crystal lattice constant, the surface morphology as well as the fluorescence properties of the as-grown films.展开更多
Ba(Mg1/3Nb2/3)O3(BMN)复合钙钛矿陶瓷具有高介电常数和高品质因子等介电性能,预示了其在光学领域的应用前景.本文采用第一性原理方法计算了BMN的电子结构,对其本征光学性能进行分析和预测.对固相合成六方相BMN的XRD测试结果进行Rietvel...Ba(Mg1/3Nb2/3)O3(BMN)复合钙钛矿陶瓷具有高介电常数和高品质因子等介电性能,预示了其在光学领域的应用前景.本文采用第一性原理方法计算了BMN的电子结构,对其本征光学性能进行分析和预测.对固相合成六方相BMN的XRD测试结果进行Rietveld精修(加权方差因子Rwp=6.73%,方差因子Rp=5.05%),在此基础上建立晶体结构模型并对其进行几何优化.运用基于密度泛函理论(DFT)的平面波赝势方法,对六方相BMN晶体模型的能带、态密度和光学性质进行理论计算.结果表明BMN的能带结构为间接带隙,禁带宽度Eg=2.728 e V.Mg-O和Ba-O以离子键结合为主,Nb-O以共价键结合为主,费米面附近的能带主要由O-2p和Nb-4d态电子占据,形成了d-p轨道杂化.修正带隙后,计算了BMN沿[100]和[001]方向上的复介电函数、吸收系数和反射率等光学性质.结果表明,BMN近乎光学各向同性,在可见光区,其本征透过率为77%<T<83%,折射率为1.91<n<2.14,并伴随一定的色散现象.实验测试结果与理论计算结果相吻合.展开更多
Barium metaborate (BaB 2O 4) exists in two forms the high temperature for m α phase and the low temperature form β phase,with the phase transition poi nt at around 925℃.The low temperature phase (β BBO) is an exce...Barium metaborate (BaB 2O 4) exists in two forms the high temperature for m α phase and the low temperature form β phase,with the phase transition poi nt at around 925℃.The low temperature phase (β BBO) is an excellent NLO cryst al for UV region,while the high temperature one (α BBO) is known as a good bir efringent material.α BBO crystal possesses better transparency in the ultravio let region compared with commonly used YVO 4 and CaCO 3 crystals.However,due t o phase transition problem,it is difficult to grow single α BBO crystals by D CZ method,which restricts its wider application.α BBO crystal usually cracks upon cooling resulted from phase transition to β phase.Therefore,it is difficult to grow β BBO single crystals directly from pure BaB 2O 4 mel t;however,to grow single crystals of α BBO is not easy,either. Our recent experiments showed that one could avoid cracking of α BBO cryst als by Sr 2+ doping.This minute amount of Sr 2+ plays the role of structure stabilizer,which inhibits structural reconstruction. In this way,Sr 2+ d op ed BBO single crystals do not subject to phase transition from 925℃ down to roo m temperature. We have mow successfully grown out α BBO single crystals 30mm in diameter by D CZ method from Sr x Ba 1- x BO 4 melt with Sr concentration 0.3 0 .5%.The ratation rate is 10 15r/min,pulling speed is 1 2mm/h.Preliminary tests revealed that its structure and physic chemical properties were almost identic al to those grown from pure melts.These Sr 2+ doped α BBO crystals are no w being widely used in optical isolators.展开更多
基金supported by grants from the China National Science and Technology Major Project (2016YFA0101203)
文摘Reprogrammed metabolism is a hallmark of cancer. Glioblastoma（GBM） tumor cells predominantly utilize aerobic glycolysis for the biogenesis of energy and intermediate nutrients. However, in GBM, the clinical significance of glycolysis and its underlying relations with the molecular features such as IDH1 mutation and subtype have not been elucidated yet. Herein, based on glioma datasets including TCGA（The Cancer Genome Atlas）, REMBRANDT（Repository for Molecular Brain Neoplasia Data） and GSE16011 we established a glycolytic gene expression signature score（GGESS） by incorporating ten glycolytic genes. Then we performed survival analyses and investigated the correlations between GGESS and IDH1 mutation as well as the molecular subtypes in GBMs. The results showed that GGESS independently predicted unfavorable prognosis and poor response to chemotherapy of GBM patients. Notably, GGESS was high in GBMs of mesenchymal subtype but low in IDH1-mutant GBMs. Furthermore, we found that the promoter regions of tumor-promoting glycolytic genes were hypermethylated in IDH1-mutant GBMs.Finally, we found that high GGESS also predicted poor prognosis and poor response to chemotherapy when investigating IDH1-wild type GBM patients only. Collectively, glycolysis represented by GGESS predicts unfavorable clinical outcome of GBM patients and is closely associated with mesenchymal subtype and IDH1 mutation in GBMs.
基金supported by Beijing Natural Science Foundation(Grant No.Z210014)National Natural Science Foundation of China(Grant No.32070543)+1 种基金National Key Research and Development Project of China(Grant No.2022YFC2303404)CAMS Innovation Fund for Medical Sciences(CIFMS)(Grant No.2022-12M-CoV19-002)
文摘Background:SARS-CoV-2,first identified in late 2019,has given rise to numerous variants of concern(VOCs),posing a significant threat to human health.The emer-gence of Omicron BA.1.1 towards the end of 2021 led to a pandemic in early 2022.At present,the lethal mouse model for the study of SARS-CoV-2 needs supplementation,and the alterations in neutrophils and monocytes caused by different strains remain to be elucidated.Methods:Human ACE2 transgenic mice were inoculated with the SARS-CoV-2 proto-type and Omicron BA.1,respectively.The pathogenicity of the two strains was evalu-ated by observing clinical symptoms,viral load and pathology.Complete blood count,immunohistochemistry and flow cytometry were performed to detect the alterations of neutrophils and monocytes caused by the two strains.Results:Our findings revealed that Omicron BA.1 exhibited significantly lower vir-ulence compared to the SARS-CoV-2 prototype in the mouse model.Additionally,we observed a significant increase in the proportion of neutrophils late in infection with the SARS-CoV-2 prototype and Omicron BA.1.We found that the proportion of monocytes increased at first and then decreased.The trends in the changes in the proportions of neutrophils and monocytes induced by the two strains were similar.Conclusion:Our study provides valuable insights into the utility of mouse models for simulating the severe disease of SARS-CoV-2 prototype infection and the milder manifestation associated with Omicron BA.1.SARS-CoV-2 prototype and Omicron BA.1 resulted in similar trends in the changes in neutrophils and monocytes.
基金supported by the Science and Technology Department of Sichuan Province(2023NSFSC1757)the"Xinglin Scholar"Talent Research Promotion Plan of Chengdu University of Traditional Chinese Medicine(grant number MPRC2021020).
文摘Diarrhea predominant irritable bowel syndrome(IBS-D)have a serious impact on the patient’s quality life due to the lack of safe and effective drugs.The transient receptor potential vanilloid subtype 1(TRPV1)is an ion channel receptor implicated in the perception of visceral injury.Recent studies indicated that TRPV1 mediates visceral hypersensitivity in IBS-D patients by enhancing the excitability of intestinal sensory neurons.Consequently,inhibiting the TRPV1 may be a promising option for the treatment of IBS-D.Current research demonstrates that various traditional Chinese medicine(TCM)methods,such as herbal prescriptions,acupuncture,and moxibustion,can reduce visceral sensitivity by regulating TRPV1 expression and its activation sensitization.This suggests that TCM methods may serve as safe and effective options for alleviating IBS-D.Therefore,this article summarizes potential therapeutic strategies of TCM as a regulator of TRPV1 for managing IBS-D.It also provides insights into potential TCM methods and natural phytochemical molecular nuclei for future drug research targeting TRPV1 in IBS-D.
基金supported by the Chinese National S&T Plan(2004BA519A55)
文摘The HA1 gene of H3N2 subtype swine influenza virus(SIV)was cloned into the expression plasmid pET-30a,the recombinant plasmid was named pET-HAl.This was transformed into E.coli BL21(DE3),and expressed by induction with IPTG.The expressed HA protein was identified by SDS-PAGE and Western blotting which showed the protein to be 42kDa and was immunoreactive.The purified HA protein was used to establish the indirect ELIS A for detection of the antibodies,specifically against the H3 subtype of SIV.The assay has excellent specificity,sensitivity and reproducibility.When 96 serum samples,randomly collected from the field,were evaluated in parallel by this new ELISA using recombinant HA1 and a routine HI test,the coincidental rate between the two tests was 86.5%.These results show that the recombinant HAl-based ELISA is specific,sensitive and easy to perform for the serological diagnosis of SIV infection.
文摘At room temperature, Ba1-xCaxMoO4 polycrystalline solid solution thin films were prepared directly in contained Ca2+ and Ba2+ alkaline solution by electrochemical anode oxidation method. The crystal phase structure, surface morphology, ratio of compositions and room temperature fluorescence properties of the as-grown films were measured by using XRD, SEM, EDAX and Fluorescence Analysis techniques, respectively. The results reveal that the ratio of composition of Ba1-xCaxMoO4 films can be modulated by controlling the ratio of Ca2+ and Ba2+ in starting solutions; moreover it has important influence of ratio of composition of the films on the grain growth, the crystal lattice constant, the surface morphology as well as the fluorescence properties of the as-grown films.
文摘Ba(Mg1/3Nb2/3)O3(BMN)复合钙钛矿陶瓷具有高介电常数和高品质因子等介电性能,预示了其在光学领域的应用前景.本文采用第一性原理方法计算了BMN的电子结构,对其本征光学性能进行分析和预测.对固相合成六方相BMN的XRD测试结果进行Rietveld精修(加权方差因子Rwp=6.73%,方差因子Rp=5.05%),在此基础上建立晶体结构模型并对其进行几何优化.运用基于密度泛函理论(DFT)的平面波赝势方法,对六方相BMN晶体模型的能带、态密度和光学性质进行理论计算.结果表明BMN的能带结构为间接带隙,禁带宽度Eg=2.728 e V.Mg-O和Ba-O以离子键结合为主,Nb-O以共价键结合为主,费米面附近的能带主要由O-2p和Nb-4d态电子占据,形成了d-p轨道杂化.修正带隙后,计算了BMN沿[100]和[001]方向上的复介电函数、吸收系数和反射率等光学性质.结果表明,BMN近乎光学各向同性,在可见光区,其本征透过率为77%<T<83%,折射率为1.91<n<2.14,并伴随一定的色散现象.实验测试结果与理论计算结果相吻合.
文摘Barium metaborate (BaB 2O 4) exists in two forms the high temperature for m α phase and the low temperature form β phase,with the phase transition poi nt at around 925℃.The low temperature phase (β BBO) is an excellent NLO cryst al for UV region,while the high temperature one (α BBO) is known as a good bir efringent material.α BBO crystal possesses better transparency in the ultravio let region compared with commonly used YVO 4 and CaCO 3 crystals.However,due t o phase transition problem,it is difficult to grow single α BBO crystals by D CZ method,which restricts its wider application.α BBO crystal usually cracks upon cooling resulted from phase transition to β phase.Therefore,it is difficult to grow β BBO single crystals directly from pure BaB 2O 4 mel t;however,to grow single crystals of α BBO is not easy,either. Our recent experiments showed that one could avoid cracking of α BBO cryst als by Sr 2+ doping.This minute amount of Sr 2+ plays the role of structure stabilizer,which inhibits structural reconstruction. In this way,Sr 2+ d op ed BBO single crystals do not subject to phase transition from 925℃ down to roo m temperature. We have mow successfully grown out α BBO single crystals 30mm in diameter by D CZ method from Sr x Ba 1- x BO 4 melt with Sr concentration 0.3 0 .5%.The ratation rate is 10 15r/min,pulling speed is 1 2mm/h.Preliminary tests revealed that its structure and physic chemical properties were almost identic al to those grown from pure melts.These Sr 2+ doped α BBO crystals are no w being widely used in optical isolators.
