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Research on Purchasing Behavior on E-commerce Platforms of Low-income People of Vietnam
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作者 Pham Tu Linh Vu Thi Tung Chi +1 位作者 Nguyen Thi Minh Nguyet Tran Van Trung 《Economics World》 2025年第1期27-38,共12页
The purpose of this study is to analyze factors affecting the purchasing behavior of low-income Vietnamese on e-commerce platforms.700 questionnaires were dispersed among universities,coffee shops,and Facebook communi... The purpose of this study is to analyze factors affecting the purchasing behavior of low-income Vietnamese on e-commerce platforms.700 questionnaires were dispersed among universities,coffee shops,and Facebook community groups in Vietnam.Respondents to the questionnaires were consumers of those places in Vietnam,which were randomly selected.Finally,SEM analysis was used on the data to test the hypotheses of the study.The study identified that“Subjective norms”and“Consumer attitudes”have no effect on shopping behavior on e-commerce platforms,while“Perceived behavioral control”is most affected and“Perceived risks”are least affected on shopping behavior. 展开更多
关键词 b2C e-commerce e-commerce platforms low-income people purchasing behavior
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SWOT Analysis on Agricultural Enterprises Implementing B2C E-commerce——A Case Study of Wuding Strong Chicken Farming Enterprises 被引量:1
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作者 Dekun WANG Wan NIE 《Asian Agricultural Research》 2017年第12期55-58,共4页
This paper first introduced current situations of agricultural enterprises using B2C e-commerce to sell agricultural products.Then,using SWOT method,it analyzed strengths,weaknesses,opportunities,and threats of Wuding... This paper first introduced current situations of agricultural enterprises using B2C e-commerce to sell agricultural products.Then,using SWOT method,it analyzed strengths,weaknesses,opportunities,and threats of Wuding strong chicken farming enterprises implementing B2C e-commerce.Finally,it came up with recommendations for Wuding strong chicken farming enterprises implementing B2C e-commerce. 展开更多
关键词 Wuding strong chicken b2C e-commerce SWOT
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Digital Marketing and B2B Cross-Border E-Commerce: A Case Study of Alibaba International Station in China 被引量:4
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作者 Qianyu Li Jiaxuan Sun 《Proceedings of Business and Economic Studies》 2023年第6期76-80,共5页
The rapid development of the“Internet+”has ushered in a new era of global digital technology innovation.Concurrent with the deepening trends of economic globalization and integration,international trade is progressi... The rapid development of the“Internet+”has ushered in a new era of global digital technology innovation.Concurrent with the deepening trends of economic globalization and integration,international trade is progressively undergoing extensive digitization,with cross-border e-commerce emerging as a significant manifestation of this transformation.Within this landscape,the cross-border e-commerce B2B platform is anticipated to become a pivotal driver for China’s cross-border trade growth,representing a strategic opportunity for trade transformation.This paper provides a comprehensive examination of the concepts,characteristics,and development status of cross-border e-commerce and B2B platforms,considering both global and Chinese perspectives.Focusing on a prominent case study,Alibaba International Station,a B2B cross-border e-commerce platform,the paper delves into its business model and core competencies,offering a thorough analysis of its role in the intricacies of international e-commerce and its contribution to the field.Drawing on insights gained,the paper concludes by presenting targeted recommendations from both the platform and government perspectives.These suggestions are informed by a nuanced understanding of the development opportunities and challenges facing China’s cross-border e-commerce B2B platforms. 展开更多
关键词 Digital economy Cross-border e-commerce b2b platform 2.0 business model analysis Alibaba International Station
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Quality Criteria for B2C E-Commerce Websites in Brazil
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作者 Thair M. Hamtini Ana Alice Vilas Boas +1 位作者 Luciana Pussi Santos Tania Regina Vasconcellos Dias 《Journal of Software Engineering and Applications》 2013年第11期589-596,共8页
The objective of this study is to compare the ranking of the quality of e-commerce B2C portals in Brazil according to explicit criteria described in the article with the ranking according to the INFO 100 ranking. Theo... The objective of this study is to compare the ranking of the quality of e-commerce B2C portals in Brazil according to explicit criteria described in the article with the ranking according to the INFO 100 ranking. Theoretical research is first described, followed by the construction of eight self-established criteria of B2C portal quality measures and identification and compilation of requirements used by the I-Best, Opinia, E-bit and other institutes. After that, a portal quality evaluation is made by the web site content analysis;finally, a comparison of the rankings is presented. The obtained results showed that in spite of the lack of knowledge of the criteria adopted by INFO100 [1], there could be agreement with the criteria adopted in this study, because there was agreement with the results for the Ponto Frio portal. Therefore, it was concluded that the eight criteria used in this study can be useful in a quality analysis of B2C electronic portals. 展开更多
关键词 b2C PORTALS CRITERIA QUALITY e-commerce
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Research on Online Reviews Impact on Commodity Sales in B2C E-commerce
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作者 Wu Guo-dong 《Review of Global Academics》 2015年第2期553-557,共5页
The objective of this paper was to analyze the impact of online reviews on sales. Based on dual path model of commodity sales, an online reviews impact on the relationship between various factors, and then the theoret... The objective of this paper was to analyze the impact of online reviews on sales. Based on dual path model of commodity sales, an online reviews impact on the relationship between various factors, and then the theoretical hypothesis of each factor has been put forward in the model. As it is intuitive and strongly supported empirically, data including Chinese texts captured from Tmall.com was utilized, and then analyzed by SPSS and ROST CM6. Our empirical study on the reviews of Tmall.com indicated that the hypotheses are verified. 展开更多
关键词 Online Review Commodity Sales b2C e-commerce Influencing Factor STATISTICALANALYSIS
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Research on Online Reviews Impact on Commodity Sales in B2C E-commerce
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作者 Wu Guodong Song Fugen 《Review of Global Academics》 2015年第1期484-487,共4页
The objective of this paper was to analyze the impact of online reviews on sales. Based on dual path model of commodity sales, an online reviews impact on the relationship between various factors, and then the theoret... The objective of this paper was to analyze the impact of online reviews on sales. Based on dual path model of commodity sales, an online reviews impact on the relationship between various factors, and then the theoretical hypothesis of each factor has been put forward in the model. As it is intuitive and strongly supported empirically, data including Chinese texts captured from Tmall. com was utilized, and then analyzed by SPSS and ROST CM6. Our empirical study on the reviews of TmalLcom indicated that the hypotheses are verified. 展开更多
关键词 Online Review Commodity Sales b2C e-commerce Influencing Factor Statistical Analysis
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Study on the Self-supporting Logistics Mode based on B2C E-commerce Enterprises
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作者 Ting LI 《International Journal of Technology Management》 2015年第2期87-89,共3页
Taking B2C E-commerce enterprises as the center, it can be divided into a set of logistics activities upstream supply chain logistics producer to B2C e-commerce enterprises, and the B2C e-commerce enterprise logistics... Taking B2C E-commerce enterprises as the center, it can be divided into a set of logistics activities upstream supply chain logistics producer to B2C e-commerce enterprises, and the B2C e-commerce enterprise logistics to client consumer, and the paper focuses on the study of self-supporting logistics for the E-commerce enterprise. According to the development status of current B2C electronic commerce enterprise in our country and logistics, the paper study B2C electric business enterprise how to correctly choose the logistics mode and how to promote the business logistics operation level. 展开更多
关键词 b2C e-commerce Logistics distribution Self-supporting Logistics Mode Operating mechanism
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Assumption of E-commerce Platform of Agricultural Products Based on F2C2B Mode
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作者 KeweiZhu 《International Journal of Technology Management》 2017年第4期86-88,共3页
This paper proposes the assumption of E-commerce platform of the agricultural products based on the F2C2B mode. The whole e-commerce model is guided by the idea of the supply chain management from the perspective of t... This paper proposes the assumption of E-commerce platform of the agricultural products based on the F2C2B mode. The whole e-commerce model is guided by the idea of the supply chain management from the perspective of the modern system, and the e-commerce integration model from customer to supplier is implemented, and the whole is optimized. E-commerce to cloud computing as the basic environment, the construction of public cloud services and private cloud resources, based on public cloud to the SaaS way to provide services to customers, focus on business search and business collaboration, make full use of the lnternet, modern communications technology to provide the real- service. The development of agricultural economy, no longer depends only on the number of some traditional agricultural resources, but also depends on the modern technology, information access and use. Through the development of basic network technology, by virtue of some modern electronic information technology and some other means that can improve the intelligence of agricultural use of the information resources, the proposed model provides the new methodology of the solution. 展开更多
关键词 F2C2b Mode e-commerce Platform Agricultural Products.
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Investigation and Countermeasures of the Development of Chinese Pharmaceutical E-commerce in the B2C Model Based on PEST-SWOT Analysis
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作者 Xiu Yu Wang Shuling 《Asian Journal of Social Pharmacy》 2023年第4期398-405,共8页
Objective To analyze the problems of pharmaceutical e-commerce and provide strategies for its development in the future B2C mode since drug network management has great potential in China.Methods By collecting,identif... Objective To analyze the problems of pharmaceutical e-commerce and provide strategies for its development in the future B2C mode since drug network management has great potential in China.Methods By collecting,identifying,and conducting literature research,PEST-SWOT identification and positioning of pharmaceutical e-commerce in the B2C mode were carried out.Results and Conclusion A PEST-SWOT analysis matrix was established to analyze the status of B2C pharmaceutical e-commerce,and to summarize its advantages,disadvantages,opportunities and threats from four perspectives of politics,economy,society and technology.Suggestions on cultivating compound talents proficient in medicine and e-commerce,exploring online payment methods for medical insurance,integration of upstream and downstream of the industrial chain and data sharing are put forward to promote the healthy and long-term development of pharmaceutical e-commerce under the background of big data. 展开更多
关键词 pharmaceutical e-commerce b2C PEST-SWOT analysis matrix
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5-O-Methylvisammioside alleviates depression-like behaviors by inhibiting nuclear factor kappa B pathway activation via targeting SRC
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作者 Wenqian Zhu Bingjin Li Ranji Cui 《Neural Regeneration Research》 2026年第8期3659-3667,共9页
Preliminary studies on emerging herbal ingredients have highlighted alternative pathways that inflammation and modulate perturbed immunity as valuable strategies for treating depression.Previous studies have shown tha... Preliminary studies on emerging herbal ingredients have highlighted alternative pathways that inflammation and modulate perturbed immunity as valuable strategies for treating depression.Previous studies have shown that 5-O-methylvisammioside,a bioactive compound derived from Saposhnikoviae Radix,possesses excellent anti-inflammatory and antioxidant biological functions,exhibits a neuroprotective effect.The purpose of this study was to explore the targets and signaling pathways of 5-O-methylvisammioside in the potential treatment of major depressive disorder using a combination of network pharmacology analysis and biological experiments.The network pharmacological analysis results indicated that the proto-oncogene tyrosine-protein kinase Src and the nuclear factor kappa B signaling pathway were highly correlated with the treatment of major depressive disorder with 5-O-methylvisammioside.Further experiments indicated that 5-O-methylvisammioside significantly improved lipopolysaccharide-induced depression-like behaviors in mice,ameliorated microglial polarization in the hippocampal CA1 and CA3 regions,and inhibited Src phosphorylation and nuclear factor kappa B pathway activation.The effects of 5-O-methylvisammioside were similar to those of the Src inhibitor PP2.When 5-O-methylvisammioside was administered with PP2,no effects were observed on lipopolysaccharide-induced depression-like behaviors in mice,nuclear factor kappa B pathway proteins,and microglial polarization.These findings indicate that 5-O-methylvisammioside may exert its antidepressant potential by inhibiting Src-mediated activation of the nuclear factor kappa B signaling pathway.Therefore,5-O-methylvisammioside might serve as a promising Chinese herbal medicine for the prevention and treatment of depression. 展开更多
关键词 5-O-methylvisammioside depression-like behavior HIPPOCAMPUS major depressive disorder MICROGLIA network pharmacology NEUROINFLAMMATION nuclear factor kappa b signaling pathway PP2 SRC
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Perturbation response scanning of drug-target networks:Drug repurposing for multiple sclerosis 被引量:1
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作者 Yitan Lu Ziyun Zhou +10 位作者 Qi Li Bin Yang Xing Xu Yu Zhu Mengjun Xie Yuwan Qi Fei Xiao Wenying Yan Zhongjie Liang Qifei Cong Guang Hu 《Journal of Pharmaceutical Analysis》 2025年第6期1277-1290,共14页
Combined with elastic network model(ENM),the perturbation response scanning(PRS)has emerged as a robust technique for pinpointing allosteric interactions within proteins.Here,we proposed the PRS analysis of drug-targe... Combined with elastic network model(ENM),the perturbation response scanning(PRS)has emerged as a robust technique for pinpointing allosteric interactions within proteins.Here,we proposed the PRS analysis of drug-target networks(DTNs),which could provide a promising avenue in network medicine.We demonstrated the utility of the method by introducing a deep learning and network perturbation-based framework,for drug repurposing of multiple sclerosis(MS).First,the MS comorbidity network was constructed by performing a random walk with restart algorithm based on shared genes between MS and other diseases as seed nodes.Then,based on topological analysis and functional annotation,the neurotransmission module was identified as the“therapeutic module”of MS.Further,perturbation scores of drugs on the module were calculated by constructing the DTN and introducing the PRS analysis,giving a list of repurposable drugs for MS.Mechanism of action analysis both at pathway and structural levels screened dihydroergocristine as a candidate drug of MS by targeting a serotonin receptor of se-rotonin 2B receptor(HTR2B).Finally,we established a cuprizone-induced chronic mouse model to evaluate the alteration of HTR2B in mouse brain regions and observed that HTR2B was significantly reduced in the cuprizone-induced mouse cortex.These findings proved that the network perturbation modeling is a promising avenue for drug repurposing of MS.As a useful systematic method,our approach can also be used to discover the new molecular mechanism and provide effective candidate drugs for other complex diseases. 展开更多
关键词 network perturbations Mechanism of action Multiple sclerosis HTR2b
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全球最大的B2B电子商务网站阿里巴巴选择Array Networks确保接入安全
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《计算机与网络》 2006年第9期33-33,共1页
近日,Array Networks在杭州宣布。与全球最大的B2B电子商务网站——阿里巴巴正式签署采购协议。此次Array SSLVPN通用访问解决方案在阿里巴巴的成功运行,有效地提升了阿里巴巴公司员工的工作效率,增加了系统访问的安全性和便捷性,... 近日,Array Networks在杭州宣布。与全球最大的B2B电子商务网站——阿里巴巴正式签署采购协议。此次Array SSLVPN通用访问解决方案在阿里巴巴的成功运行,有效地提升了阿里巴巴公司员工的工作效率,增加了系统访问的安全性和便捷性,大大节约了时间成本,是Array SPX产品与中国的电子商务服务提供商(SP)的又一次成功合作。 展开更多
关键词 networkS 电子商务网站 b2b 服务提供商 工作效率 系统访问 时间成本 便捷性
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木犀草素通过与B淋巴细胞瘤-2蛋白结合抑制硅肺纤维化的作用机制 被引量:2
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作者 李芸芸 丁选胜 +6 位作者 周家伟 刘紫琴 杨雪莲 刘亚锋 郭健强 胡东 吴静 《兰州大学学报(医学版)》 2024年第8期7-15,共9页
目的探究中药丹参有效成分在治疗硅肺中的作用和作用机制。方法运用网络药理学和分子对接技术筛选出中药丹参治疗硅肺的有效成分和关键靶点。热转移实验检测有效成分与关键靶点的结合稳定性,细胞划痕实验检测上皮细胞的迁移能力,蛋白质... 目的探究中药丹参有效成分在治疗硅肺中的作用和作用机制。方法运用网络药理学和分子对接技术筛选出中药丹参治疗硅肺的有效成分和关键靶点。热转移实验检测有效成分与关键靶点的结合稳定性,细胞划痕实验检测上皮细胞的迁移能力,蛋白质印迹法检测上皮间质转化的标志蛋白、纤维化标志蛋白以及凋亡相关蛋白的表达水平。结果中药丹参的有效成分木犀草素与硅肺关键靶点B淋巴细胞瘤-2(BCL-2)具有最低的结合能,热转移实验验证木犀草素与BCL-2蛋白具有较好的结合稳定性。细胞划痕实验显示木犀草素能够抑制转化生长因子-β1刺激的上皮细胞迁移。蛋白质印迹法证明木犀草素能够抑制BCL-2蛋白的表达,与转化生长因子-β1刺激组相比,木犀草素给药及沉默BCL-2后能够抑制上皮间质转化及胶原蛋白的形成,进而抑制纤维化的进程。木犀草素给药及沉默BCL-2后能够促进细胞凋亡。结论木犀草素能够与BCL-2结合进而抑制硅肺纤维化的进程,其作用机制可能与木犀草素能够促进细胞凋亡有关。 展开更多
关键词 网络药理学 硅肺 木犀草素 b淋巴细胞瘤-2 上皮间质转化 细胞凋亡
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Innovation and Development of B2C Cross-Border E-Commerce from the Perspective of Economic Globalization
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作者 Jiekun Wu 《Proceedings of Business and Economic Studies》 2026年第3期123-128,共6页
From the perspective of economic globalization,B2C cross-border e-commerce has emerged as a promising sector.Industries can leverage internet platforms to engage in international trade,exporting goods or services over... From the perspective of economic globalization,B2C cross-border e-commerce has emerged as a promising sector.Industries can leverage internet platforms to engage in international trade,exporting goods or services overseas to expand their global influence.However,this model also presents challenges.Many cross-border e-commerce businesses lack comprehensive global operation strategies,relying solely on B2C platforms for marketing.International consumers exhibit cultural differences and consumption habits that make domestic brand strategies insufficient for global marketing needs.The industry faces compliance risks and data security threats,potentially causing financial losses.Additionally,many companies fail to establish effective talent acquisition and development models,leading to talent shortages that undermine cross-border marketing capabilities.To align with globalization trends,the cross-border e-commerce sector should innovate based on B2B operational characteristics to ensure sustainable development.This article examines the current status and challenges of B2C cross-border e-commerce under economic globalization,and proposes innovative development pathways to enhance China’s B2C cross-border e-commerce capabilities and support industry growth. 展开更多
关键词 Economic globalization b2C cross-border e-commerce Innovation and development
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The Effects of B2C Interaction on Customer Loyalty
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作者 xinyue yang qinjian yuan 《Journal of Data and Information Science》 CSCD 2018年第2期78-104,共27页
Purpose: This research attempts to examine the relationship between B2C interaction and customer loyalty in Business-to-Customer(B2C) context from a new perspective of the interactive tool.Design/methodology/appro... Purpose: This research attempts to examine the relationship between B2C interaction and customer loyalty in Business-to-Customer(B2C) context from a new perspective of the interactive tool.Design/methodology/approach: The scale for B2C interactive tools is of seven dimensions: efficiency, security, fulfillment, mobility, community, cultivation, and customization. A model reflecting the influences of these attributes on customer loyalty is developed and empirically examined based on data collected from 265 B2C customers. Findings: Results reveal that the fulfillment, mobility, community, and customization of B2C interactive tools can enhance customer loyalty directly and significantly. Efficiency and security, serving as the premise for possible purchase behavior, facilitate fulfillment. In addition, cultivation promotes the formation of customization, which directly strengthens customer loyalty.Research limitations: Models considering individual-level indicators and combined with classic loyalty mechanisms in B2C context may lead to a deeper understanding of the tested effects of interaction on customer loyalty.Practical implications: To strengthen B2C interaction and further cultivate loyal customers, making interactive tools more fundamental, flexible, and personalized is critical for B2C enterprises. Originality/value: This study proposes a new perspective from interactive tools when measuring the relationship between B2C interaction and customer loyalty, and offers a useful theoretical lens and reasonable explanations for investigating customer loyalty in B2C e-commerce context. 展开更多
关键词 b2C e-commerce INTERACTION Interactive tool Customer loyalty
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Risk Analysis and Consumer Protection in B2C Transactions
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作者 杨坚争 时启亮 +1 位作者 Gary Millar Ruhul A.Sarker 《Journal of Donghua University(English Edition)》 EI CAS 2005年第1期83-89,共7页
Recent studies have shown that the perceived lack of security is a major obstacle to the wider acceptance of e-commerce. To overcome this barrier, businesses need to implement comprehensive consumer protection systems... Recent studies have shown that the perceived lack of security is a major obstacle to the wider acceptance of e-commerce. To overcome this barrier, businesses need to implement comprehensive consumer protection systems that protect consumers during every stage of the purchasing process. This paper used the consumer behaviour model as the basis for analysing risks in Bussiness-to-Consumer (B2C) transactions. Four categories of risks were identified: information, agreement, payment and delivery risk. By combining these risk categories with the three dimensions of management, technology and legislation, a comprehensive B2C consumer protection framework is developed. 展开更多
关键词 Consumer Protection business-to-Consumer. (b2C) e-commerce Security Risk.
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人工神经网络应用于维生素B族4组分同时测定 被引量:8
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作者 何池洋 孙益民 +1 位作者 陈友存 吴根华 《浙江大学学报(理学版)》 CAS CSCD 2002年第2期160-165,共6页
应用人工神经网络原理 ,采用 Levenberg- Marquardt BP算法 ,对于吸收光谱严重重叠的维生素B1,B2 ,B6和烟酰胺 4组分同时进行含量测定 .四者的平均回收率分别为 10 0 % ,98% ,96 % ,10 0 % .该方法用于复合维生素 B片剂中 B1,B2 ,B6和... 应用人工神经网络原理 ,采用 Levenberg- Marquardt BP算法 ,对于吸收光谱严重重叠的维生素B1,B2 ,B6和烟酰胺 4组分同时进行含量测定 .四者的平均回收率分别为 10 0 % ,98% ,96 % ,10 0 % .该方法用于复合维生素 B片剂中 B1,B2 ,B6和烟酰胺的同时测定 ,获得较满意的结果 .实验表明 ,该方法与现有的算法相比具有训练速度快、预测结果准确度高等特点 ,有望能成为多组分分析的有效方法之一 . 展开更多
关键词 人工神经网络 维生素b1 维生素b2 维生素b6 烟酰胺 同时测定 含量测定 多组分分析
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弥漫大B细胞淋巴瘤R-CHOP化疗的生存预后评估模型 被引量:1
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作者 翁翔 赵明哲 胡慧仙 《浙江医学》 CAS 2024年第2期172-176,共5页
目的建立弥漫大B细胞淋巴瘤(DLBCL)R-CHOP化疗的生存预后评估模型,以指导临床进行风险分层。方法选取2018年1月至2023年1月在金华市中心医院确诊的DLBCL患者210例为研究对象,根据2:1抽样比例随机分为建模集140例和验证集70例。所有患者... 目的建立弥漫大B细胞淋巴瘤(DLBCL)R-CHOP化疗的生存预后评估模型,以指导临床进行风险分层。方法选取2018年1月至2023年1月在金华市中心医院确诊的DLBCL患者210例为研究对象,根据2:1抽样比例随机分为建模集140例和验证集70例。所有患者均接受R-CHOP化疗方案至少4个疗程(21 d为1个疗程),常规随访至2023年6月。根据生存预后分为良好组和不良组。比较两组患者的临床资料[包括性别、年龄、BMI、基础疾病、血清乳酸脱氢酶(LDH)、美国国家综合癌症网络国际预后指数(NCCN-IPI)]、病理特征[包括Hans分型、Ann Arbor分期、原发部位、Ki-67阳性表达率、MYC和B细胞淋巴瘤(Bcl)-2蛋白阳性表达以及骨髓侵犯]、治疗(化疗疗程和并发症)和随访时间。结果建模集中良好组105例和不良组35例,预后不良发生率25.0%;验证集良好组55例和不良组15例,不良发生率21.4%。建模集中不良组年龄大于良好组,LDH水平、NCCN-IPI、Ann Arbor分期Ⅲ~Ⅳ比例、Ki-67阳性表达率、MYC和Bcl-2蛋白双阳性表达率和骨髓侵犯比例均高于良好组,差异均有统计学意义(均P<0.05)。多因素Cox回归分析显示,NCCN-IPI(HR=2.526,95%CI:2.001~3.125,P<0.001)、Ann Arbor分期Ⅲ~Ⅳ(HR=5.021,95%CI:4.125~5.998,P<0.001)、MYC和Bcl-2蛋白双阳性(HR=3.859,95%CI:3.256~4.754,P<0.001)均是DLBCL患者R-CHOP化疗预后不良的危险因素。建立预测模型Y=0.056+1.032×(NCCN-IPI)+1.986×(Ann Arbor分期)+1.434×(MYC和Bcl-2蛋白双阳性)。ROC曲线显示,模型预测建模集与验证集预后不良的AUC分别为0.923和0.866(均P<0.01)。结论DLBCL患者化疗前NCCN-IPI、Ann Arbor分期升高以及MYC和Bcl-2蛋白双阳性表达与R-CHOP化疗预后不良密切相关,通过建立量化预测模型能够辅助临床早期、准确识别预后不良的高危群体,有较好的应用价值。 展开更多
关键词 MYC b细胞淋巴瘤-2 弥漫大b细胞淋巴瘤 模型 美国国家综合癌症网络国际预后指数
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基于PI3K/Akt通路探讨黄连知母汤治疗2型糖尿病的作用机制 被引量:3
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作者 王磊 潘蕴 +2 位作者 万利花 涂文玲 曹灵勇 《中国实验方剂学杂志》 北大核心 2025年第21期168-177,共10页
目的:基于磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路探讨黄连知母汤改善2型糖尿病(T2DM)糖脂代谢紊乱及肝脏胰岛素抵抗(IR)的作用及可能的分子机制。方法:采用高脂饮食喂养诱导Goto-Kakizaki(GK)大鼠制备T2DM模型,随机分为正常组... 目的:基于磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路探讨黄连知母汤改善2型糖尿病(T2DM)糖脂代谢紊乱及肝脏胰岛素抵抗(IR)的作用及可能的分子机制。方法:采用高脂饮食喂养诱导Goto-Kakizaki(GK)大鼠制备T2DM模型,随机分为正常组、模型组、二甲双胍组(0.10 g·kg^(-1))、黄连知母汤组(3.60 g·kg^(-1)),每组8只;给予药物连续干预8周,收集各组大鼠血清、肝脏组织。酶联免疫吸附测定法(ELISA)测定大鼠空腹胰岛素(FINS)水平,并计算胰岛素抵抗指数(HOMA-IR);采用全自动生化分析仪测定大鼠总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;苏木素-伊红(HE)染色进行肝组织病理观察;ELISA检测大鼠血清中白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量。结合网络药理学及转录组学测序分析正常组、模型组、黄连知母汤组肝组织差异表达基因(DEGs),并进行京都基因和基因组百科全书(KEGG)通路富集分析获得黄连知母汤干预T2DM差异表达通路。实时荧光定量聚合酶链式反应法(Real-time PCR)检测肝组织胰岛素受体底物-1(IRS-1)、PI3K、Akt、过氧化物酶体增殖物激活受体γ(PPARγ) mRNA表达,蛋白免疫印迹法(Western blot)检测肝组织IRS-1、PI3K、Akt、PPARγ蛋白表达。结果:黄连知母汤干预8周后能够显著改善T2DM大鼠模型多饮、多食、多尿等典型症状(P<0.01),降低T2DM大鼠空腹血糖水平和胰岛素抵抗(P<0.01);病理切片结果显示黄连知母汤有效改善了肝组织的脂肪变性和炎症水肿程度,减少脂质空泡;生化检测结果表明黄连知母汤有效降低了TC、TG、LDL-C水平(P<0.01);ELISA检测结果显示黄连知母汤有效降低血清炎症因子IL-6、TNF-α的含量(P<0.05)。结合网络药理学预测结果及转录组学KEGG通路分析提示黄连知母汤组与模型组比较差异基因显著富集于PI3K/Akt等信号通路。Real-time PCR和Western blot结果确证黄连知母汤可有效上调肝组织中PI3K/Akt信号通路相关mRNA及蛋白表达(P<0.05,P<0.01),发挥抗炎、减少肝脏脂质蓄积、增加胰岛素敏感性的作用。结论:黄连知母汤能有效改善T2DM糖脂代谢紊乱,其机制可能与调控PI3K/Akt通路,改善炎症,减少肝脏脂质沉积,减轻肝脏胰岛素抵抗有关。 展开更多
关键词 黄连知母汤 2型糖尿病 磷脂酰肌醇3-激酶/蛋白激酶b信号通路 网络药理学 转录组学
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基于生物信息学和实验联合探讨熟地黄治疗2型糖尿病的潜在机制 被引量:10
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作者 任婧 卜竹林 +2 位作者 李晓芬 孟祥龙 张朔生 《中草药》 北大核心 2025年第2期566-584,共19页
目的 运用生物信息学、网络药理学及实验验证的方法探究熟地黄Rehmanniae Radix Praeparata治疗2型糖尿病(type 2 diabetes mellitus,T2DM)的作用机制。方法 熟地黄的水提物ig给予T2DM小鼠,并通过超高效液相色谱-高分辨质谱(UPLC-HRMS)... 目的 运用生物信息学、网络药理学及实验验证的方法探究熟地黄Rehmanniae Radix Praeparata治疗2型糖尿病(type 2 diabetes mellitus,T2DM)的作用机制。方法 熟地黄的水提物ig给予T2DM小鼠,并通过超高效液相色谱-高分辨质谱(UPLC-HRMS)对入血及入胰腺成分进行辨识,在瑞士目标预测数据库(Swiss Target Prediction)预测潜在靶点,并与基因表达综合数据库(GeneExpressionOmnibus,GEO)中GSE15932、GSE25724数据集及比较毒物基因组学数据库(Comparative Toxicogenomics Database,CTD)、人类基因数据库(GeneCards)取交集获取共同基因,并在Cytoscape中进行聚类分析,得到熟地黄入血和入胰腺靶点。对熟地黄入血和入胰腺的靶点取交集分析。在ADMETab3.0数据库中进行毒性预测、分子对接及信使核糖核酸(messenger ribonucleic acid,m RNA)-微小核糖核酸(microRNA,miRNA)-长链非编码核糖核酸(long non-coding RNA,lncRNA)互作网络分析,运用基因和蛋白相互作用和功能数据库(GeneMANIA)分析主要生物功能,并结合实时荧光定量PCR实验验证。结果 熟地黄可改善T2DM小鼠异常葡萄糖水平和糖耐受量。熟地黄入血化合物138个,相对应的潜在基因靶点分别为18个和1个聚类;入胰腺化合物为359个,相对应的潜在基因靶点分别为141个和6个聚类。甲状腺素转运蛋白基因(transthyretin,TTR)和ATP结合盒转运蛋白B1(ATP binding cassette subfamily B member 1,ABCB1)为熟地黄入血及胰腺的交集基因靶点;得到包含6个相同miRNA及14个相同lncRNA的m RNA-miRNAlncRNA互作网络。主要生物功能涉及脂质运输和代谢、药物运输、离子和神经递质运输、血管过程及炎症反应的调节等。熟地黄可影响T2DM小鼠血液和胰腺中TTR、ABCB1的m RNA表达水平。结论 TTR和ABCB1可被视为熟地黄治疗T2DM的潜在作用靶点,为T2DM治疗提供依据的同时为后续深入研究熟地黄作用于T2DM的相关问题提供思路。 展开更多
关键词 生物信息学 网络药理学 2型糖尿病 熟地黄 甲状腺素转运蛋白基因 ATP结合盒转运蛋白b1
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