枯草芽孢杆菌B25是对香蕉枯萎病镰刀菌有较好拮抗作用的一株生防菌。采用70%饱和(NH_4)_2SO_4沉淀法获得的B25的发酵液粗提物作用于病原菌,能使菌丝或孢子肿大、扭曲或畸形。粗提物质通过DEAE-Sepharose fast flow离子交换层析与Sephade...枯草芽孢杆菌B25是对香蕉枯萎病镰刀菌有较好拮抗作用的一株生防菌。采用70%饱和(NH_4)_2SO_4沉淀法获得的B25的发酵液粗提物作用于病原菌,能使菌丝或孢子肿大、扭曲或畸形。粗提物质通过DEAE-Sepharose fast flow离子交换层析与Sephadex G-100凝胶层析进行分离纯化,经SDS-PAGE电泳检测得到了单一的条带,再通过Nano LC-ESI-MS/MS质谱分析,获得了17个肽段的氨基酸序列。NCBI数据库检索,与之序列相似性最高的仅为37%,可能是一种新的抗真菌蛋白。展开更多
Satisfactory single crystals of deshexapeptide(B25—B30) insulin for X-ray crystal structure analysis have been grown in citrate buffer by the method of hanging-drop gas phase diffusion. The crystal belongs to the mon...Satisfactory single crystals of deshexapeptide(B25—B30) insulin for X-ray crystal structure analysis have been grown in citrate buffer by the method of hanging-drop gas phase diffusion. The crystal belongs to the monoclinic system with space group C2. The unit cell constants are α=42.6, b=37.9, c=27.2, β=125.4 and there is only one molecule of deshexapeptide insulin in an asymmetric unit.展开更多
文摘枯草芽孢杆菌B25是对香蕉枯萎病镰刀菌有较好拮抗作用的一株生防菌。采用70%饱和(NH_4)_2SO_4沉淀法获得的B25的发酵液粗提物作用于病原菌,能使菌丝或孢子肿大、扭曲或畸形。粗提物质通过DEAE-Sepharose fast flow离子交换层析与Sephadex G-100凝胶层析进行分离纯化,经SDS-PAGE电泳检测得到了单一的条带,再通过Nano LC-ESI-MS/MS质谱分析,获得了17个肽段的氨基酸序列。NCBI数据库检索,与之序列相似性最高的仅为37%,可能是一种新的抗真菌蛋白。
基金Project supported by the National Natural Science Foundation of China.
文摘Satisfactory single crystals of deshexapeptide(B25—B30) insulin for X-ray crystal structure analysis have been grown in citrate buffer by the method of hanging-drop gas phase diffusion. The crystal belongs to the monoclinic system with space group C2. The unit cell constants are α=42.6, b=37.9, c=27.2, β=125.4 and there is only one molecule of deshexapeptide insulin in an asymmetric unit.
