[Objectives]To study the protective effect and mechanism of n-butanol extract of Diploclisia glaucescens(B1.)Diels on rats with adjuvant arthritis.[Methods]A rat adjuvant arthritis(AA)model with similarities to a clin...[Objectives]To study the protective effect and mechanism of n-butanol extract of Diploclisia glaucescens(B1.)Diels on rats with adjuvant arthritis.[Methods]A rat adjuvant arthritis(AA)model with similarities to a clinical RA(rheumatoid arthritis)patient was used,and the model was made by injection of Complete Freund s adjuvant(CFA).Body mass and joint swelling degree were used as indicators,and the organ index was calculated and the synovial tissue of rats was examined under microscope to evaluate the protective effect of n-butanol extract on arthritis.The effects of n-butanol extract on TNF-α,IL-1βand PGE_(2)contents in rat serum were detected by ELISA kit.[Results]Arthritic rats experienced significant weight loss;the n-butanol extract reduced the joint swelling in rats.It exerted an effect on rat organs and reduced the contents of TNF-α,IL-1βand PGE_(2) in rat serum,and also reduced synovial inflammation in rats.[Conclusions]The n-butanol extract of D.glaucescens can protect rats with adjuvant arthritis by reducing the content of inflammatory factors.展开更多
Three new ent-kaurane diterpenoids fimbrialtols K–M（3–5） with highly substituted functionalities were isolated from the extract of Flickingeria fimbriata（B1.） Hawkes. The structures of these new compounds were d...Three new ent-kaurane diterpenoids fimbrialtols K–M（3–5） with highly substituted functionalities were isolated from the extract of Flickingeria fimbriata（B1.） Hawkes. The structures of these new compounds were determined by NMR and HR-ESIMS. The relative configurations of these compounds were determined by analysis of NOESY correlations. The absolute configurations of these new compounds were established by CD methods together with considering the biosynthetic pathway. Compounds 3 and4 contain a cinnamic carboxyl group, whereas compound 5 possesses a benzoic carboxyl group representing the first report in more than known 600 ent-kaurane diterpenoids.展开更多
目的分析首发精神分裂症患者认知水平与血清高迁移率族蛋白B1(high mobil ity group protein B1,HMGB1)、胃饥饿素(Ghrelin)、程序性细胞死亡因子4(programmed cell death factor 4,PDCD4)的相关性。方法选定乌鲁木齐市第四人民医院2021...目的分析首发精神分裂症患者认知水平与血清高迁移率族蛋白B1(high mobil ity group protein B1,HMGB1)、胃饥饿素(Ghrelin)、程序性细胞死亡因子4(programmed cell death factor 4,PDCD4)的相关性。方法选定乌鲁木齐市第四人民医院2021年6月~2023年6月就诊的60例首发精神分裂症患者为研究对象,以MATRICS公认认知成套测验(MATRICS consensus cognitive battery,MCCB)量表对患者进行认知功能评价,以MCCB量表评价患者认知功能并分组,比较认知功能正常组(39例)、认知功能障碍组(21例)阳性阴性症状评定量表(PANSS)评分、血清HMGB1、Ghrelin、PDCD4水平的差异,Pearson分析血清HMGB1、Ghrelin、PDCD4与MCCB评分、PANSS评分的相关性。结果认知功能障碍组MCCB评分、血清Ghrelin、PDCD4水平均低于认知功能正常组(P<0.05);认知功能障碍组PANSS评分、血清HMGB1均高于认知功能正常组(P<0.05);血清HMGB1与MCCB评分呈负相关性(P<0.05,r值=-0.394),与PANSS评分呈正相关性(P<0.05,r值=0.405),血清Ghrelin、PDCD4与MCCB评分均呈正相关性(P均<0.05,r值=0.386、0.412),血清Ghrelin、PDCD4与PANSS评分均呈负相关性(P<0.05,r值=-0.392、-0.402)。结论首发精神分裂症患者血清Ghrelin、PDCD4水平较低,血清HMGB1水平较高,血清HMGB1、Ghrelin、PDCD4与首发精神分裂症患者认知功能具有相关性。展开更多
基金Supported by Guangxi First-class Discipline Construction Project(2018xk0602019xk105)+2 种基金Open Project of Guangxi Key Laboratory of Zhuang and Yao Medicine(GXZYKF2019-2,GXZYKF2022-11)Research Project of Guangxi Key Laboratory of Pharmacodynamic Research of Traditional Chinese Medicine(19-050-39-A6)Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6).
文摘[Objectives]To study the protective effect and mechanism of n-butanol extract of Diploclisia glaucescens(B1.)Diels on rats with adjuvant arthritis.[Methods]A rat adjuvant arthritis(AA)model with similarities to a clinical RA(rheumatoid arthritis)patient was used,and the model was made by injection of Complete Freund s adjuvant(CFA).Body mass and joint swelling degree were used as indicators,and the organ index was calculated and the synovial tissue of rats was examined under microscope to evaluate the protective effect of n-butanol extract on arthritis.The effects of n-butanol extract on TNF-α,IL-1βand PGE_(2)contents in rat serum were detected by ELISA kit.[Results]Arthritic rats experienced significant weight loss;the n-butanol extract reduced the joint swelling in rats.It exerted an effect on rat organs and reduced the contents of TNF-α,IL-1βand PGE_(2) in rat serum,and also reduced synovial inflammation in rats.[Conclusions]The n-butanol extract of D.glaucescens can protect rats with adjuvant arthritis by reducing the content of inflammatory factors.
基金financially supported by the Chinese National S&T Special Project on Major New Drug Innovation(No.2013ZX09508104)the Fundamental Research Funds for the Central Scientific Research Institutes for Public WelfareCAMS Innovation Fund for Medical Sciences(CIFMS,No.2016-I2M-3-015)
文摘Three new ent-kaurane diterpenoids fimbrialtols K–M（3–5） with highly substituted functionalities were isolated from the extract of Flickingeria fimbriata（B1.） Hawkes. The structures of these new compounds were determined by NMR and HR-ESIMS. The relative configurations of these compounds were determined by analysis of NOESY correlations. The absolute configurations of these new compounds were established by CD methods together with considering the biosynthetic pathway. Compounds 3 and4 contain a cinnamic carboxyl group, whereas compound 5 possesses a benzoic carboxyl group representing the first report in more than known 600 ent-kaurane diterpenoids.
文摘目的分析首发精神分裂症患者认知水平与血清高迁移率族蛋白B1(high mobil ity group protein B1,HMGB1)、胃饥饿素(Ghrelin)、程序性细胞死亡因子4(programmed cell death factor 4,PDCD4)的相关性。方法选定乌鲁木齐市第四人民医院2021年6月~2023年6月就诊的60例首发精神分裂症患者为研究对象,以MATRICS公认认知成套测验(MATRICS consensus cognitive battery,MCCB)量表对患者进行认知功能评价,以MCCB量表评价患者认知功能并分组,比较认知功能正常组(39例)、认知功能障碍组(21例)阳性阴性症状评定量表(PANSS)评分、血清HMGB1、Ghrelin、PDCD4水平的差异,Pearson分析血清HMGB1、Ghrelin、PDCD4与MCCB评分、PANSS评分的相关性。结果认知功能障碍组MCCB评分、血清Ghrelin、PDCD4水平均低于认知功能正常组(P<0.05);认知功能障碍组PANSS评分、血清HMGB1均高于认知功能正常组(P<0.05);血清HMGB1与MCCB评分呈负相关性(P<0.05,r值=-0.394),与PANSS评分呈正相关性(P<0.05,r值=0.405),血清Ghrelin、PDCD4与MCCB评分均呈正相关性(P均<0.05,r值=0.386、0.412),血清Ghrelin、PDCD4与PANSS评分均呈负相关性(P<0.05,r值=-0.392、-0.402)。结论首发精神分裂症患者血清Ghrelin、PDCD4水平较低,血清HMGB1水平较高,血清HMGB1、Ghrelin、PDCD4与首发精神分裂症患者认知功能具有相关性。
