In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detectio...In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.展开更多
To improve the economic benefits and farmers’enthusiasm of Brassica napus production,this study compared the agronomic traits,economic traits,and incomes from vegetable and oilseed among five early maturing varieties...To improve the economic benefits and farmers’enthusiasm of Brassica napus production,this study compared the agronomic traits,economic traits,and incomes from vegetable and oilseed among five early maturing varieties of Brassica napus,thereby selecting the suitable materials for breeding the varieties for both vegetable and oil.The results showed that all of the five varieties had low erucic acid in oil and low glucosinolate in rapeseed meal.The economic benefits of the varieties for vegetable and oil were generally higher than those of the varieties for only oil.‘CNT01’had the best economic benefits,with the vegetable yield of 8175.3 kg/hm^(2),the rapeseed yield of 2476.5 kg/hm^(2),the vegetable income of 14859.0 yuan/hm^(2),the rapeseed income of 32701.2 yuan/hm^(2),and the net income of 35560.2 yuan/hm^(2).‘CNZ01’was selected as the variety with highest oil quality,with the rapeseed yield of 2712.6 kg/hm^(2) and the net income of 11775.6 yuan/hm^(2).In conclusion,‘CNT01’was selected as the variety for both vegetable and oil.展开更多
A breeding strategy for widening the germplasm of Brassica napus was proposed by introgression of the A^r subgenome of B. rapa （A^rA^r） and C^c of B. carinata （B^cB^cC^cC^c） into natural B. napus （AnAncncn）. The...A breeding strategy for widening the germplasm of Brassica napus was proposed by introgression of the A^r subgenome of B. rapa （A^rA^r） and C^c of B. carinata （B^cB^cC^cC^c） into natural B. napus （AnAncncn）. The progenies with 38 chromosomes that were derived from the self-pollinated seeds of pentaploid hybrids （A^rA^nB^cC^cC^n） were used for further research. Some of the partial new-typed B. napus showed normal meiotic behavior, high portion of germinated pollen and normal embryological development. This indicates that the selected new-typed B. napus had a balanced genetic base. Molecular analysis showed that about 50% of the genome in the new-typed B. napus was replaced by A^r and C^c subgenome from B. rapa and B. carinata. Considering the genetic diversity among different lines of new-typed B. napus, it was deduced that the introgression of the genomic components from B, rapa and B. carinata could widen the genetic diversity of rapeseed.展开更多
[Objective] The aim of this work was to analyze the N fertilization on the vegetative growth and N uptake of different winter rapeseed varieties at wintering stage. [Method] In two consecutive years （2009-2011）, two...[Objective] The aim of this work was to analyze the N fertilization on the vegetative growth and N uptake of different winter rapeseed varieties at wintering stage. [Method] In two consecutive years （2009-2011）, two winter rapeseed varieties （B. napus L.）, an early maturity variety Zhongyou 116 （ZY116） and a middle-late application maturity variety Zhongyouza 12 （ZYZ12） were employed. Field experiments with different N levels （0, 90, 180, 270, 360 kg N/hm 2 ） were designed. At the wintering stage, the dry matter weight, the nitrogen content and concentration of plants, leaf nitrate reductase activity （NRA） and seed yields were investigated. [Result] The shoot dry matter of ZY116 increased rapidly when N rate ranged from 0 to 180 kg/hm 2 , and it raised slightly when N rate ranged from 180 to 360 kg/hm 2 . The shoot dry matter of ZYZ12 were changed in a single peak curve; the peak of shoot dry matter appeared at 270 kg N/hm 2 . The N concentration and N content in shoot and root increased rapidly when the N rate changed from 90 to 180 kg/hm 2 . Moreover, the N concentration and N content root of in ZYZ12 were much higher than that of ZY116. Present study revealed that the changed trend of leaf nitrate reductase activities （NRA） were significantly increased at the N rate of 180 kg/hm 2 in ZY116 and ZYZ12 compared with the N rate of 90 kg/hm 2 in two years. [Conclusion] Optimal nitrogen application significantly increased the dry weights and N uptake at wintering stage as well as increasing the yield of winter oilseed rape.展开更多
Nitrogen(N)assimilation is a wide pathway in plants because of its fundamental importance for growth and development.The transport,assimilation and recycling of nitrogen is a highly complex and regulated process,as it...Nitrogen(N)assimilation is a wide pathway in plants because of its fundamental importance for growth and development.The transport,assimilation and recycling of nitrogen is a highly complex and regulated process,as it is the mineral nutrient that is required in great abundance by the plants.Basic approach to enhance agriculture sustainability is dependent on exploration of the elite germplasm where new cultivars could perform better even under low N.To test the effect of nitrogen levels at 100 and 125 kg ha-1 on photosynthetic pigments and N assimilation in oilseed rape(B.napus L.)canola variety GSC-7 and hybrid Hyola PAC 401 were selected for comparison with new hybrid PGSH-52 in pipeline.N assimilating enzymes were assayed at vegetative,flowering and siliquing stages of crop growth to visualise the impact of N on the productivity.Nitrogen assimilating enzymes were highest at flowering stage and enhanced with increased N level.Nitrate reductase(NR)activity improved by 11.5%,nitrite reductase(NiR)by 24.2%,glutamine synthetase(GS)by 12.2%and glutamate synthase(GOGAT)by 35.2%over recommended N dose(N100)at flowering stage.Hyola PAC 401 registered maximum enzymatic activities trailed by GSC-7 at all the three stages of crop growth.Differences existed within the genotypes for photosynthetic pigments which varied with N levels.Chlorophyll a,chlorophyll b,total chlorophyll and carotenoids increased with nitrogen at 125 kg N ha^(-1)whereas chlorophyll a/b declined at three stages of crop growth.At flowering total chlorophyll and carotenoids were maximum and enhanced by 12.8%and 5%respectively with higher nitrogen level.展开更多
基金the financial support provided by project of Science and Technology Tackling Key Problems in Henan Province(Grant No.182102110430)the National Key Research and Development Program of China(Grant No.2016YFD0101300)by China Agricultural Research system(CARS-12).
文摘In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.
文摘To improve the economic benefits and farmers’enthusiasm of Brassica napus production,this study compared the agronomic traits,economic traits,and incomes from vegetable and oilseed among five early maturing varieties of Brassica napus,thereby selecting the suitable materials for breeding the varieties for both vegetable and oil.The results showed that all of the five varieties had low erucic acid in oil and low glucosinolate in rapeseed meal.The economic benefits of the varieties for vegetable and oil were generally higher than those of the varieties for only oil.‘CNT01’had the best economic benefits,with the vegetable yield of 8175.3 kg/hm^(2),the rapeseed yield of 2476.5 kg/hm^(2),the vegetable income of 14859.0 yuan/hm^(2),the rapeseed income of 32701.2 yuan/hm^(2),and the net income of 35560.2 yuan/hm^(2).‘CNZ01’was selected as the variety with highest oil quality,with the rapeseed yield of 2712.6 kg/hm^(2) and the net income of 11775.6 yuan/hm^(2).In conclusion,‘CNT01’was selected as the variety for both vegetable and oil.
基金This study was supported by High Project of Science and Technology in China (No. 2001AA21110103)Doctoral Foundation of Education Department in China (No. 20020504009).
文摘A breeding strategy for widening the germplasm of Brassica napus was proposed by introgression of the A^r subgenome of B. rapa （A^rA^r） and C^c of B. carinata （B^cB^cC^cC^c） into natural B. napus （AnAncncn）. The progenies with 38 chromosomes that were derived from the self-pollinated seeds of pentaploid hybrids （A^rA^nB^cC^cC^n） were used for further research. Some of the partial new-typed B. napus showed normal meiotic behavior, high portion of germinated pollen and normal embryological development. This indicates that the selected new-typed B. napus had a balanced genetic base. Molecular analysis showed that about 50% of the genome in the new-typed B. napus was replaced by A^r and C^c subgenome from B. rapa and B. carinata. Considering the genetic diversity among different lines of new-typed B. napus, it was deduced that the introgression of the genomic components from B, rapa and B. carinata could widen the genetic diversity of rapeseed.
基金Supported by the Special Funds for Modern Agricultural (oilseed rape) Technical System (MATS) of Chinathe National Natural Science Foundation of China (NSFC) (31071372)~~
文摘[Objective] The aim of this work was to analyze the N fertilization on the vegetative growth and N uptake of different winter rapeseed varieties at wintering stage. [Method] In two consecutive years （2009-2011）, two winter rapeseed varieties （B. napus L.）, an early maturity variety Zhongyou 116 （ZY116） and a middle-late application maturity variety Zhongyouza 12 （ZYZ12） were employed. Field experiments with different N levels （0, 90, 180, 270, 360 kg N/hm 2 ） were designed. At the wintering stage, the dry matter weight, the nitrogen content and concentration of plants, leaf nitrate reductase activity （NRA） and seed yields were investigated. [Result] The shoot dry matter of ZY116 increased rapidly when N rate ranged from 0 to 180 kg/hm 2 , and it raised slightly when N rate ranged from 180 to 360 kg/hm 2 . The shoot dry matter of ZYZ12 were changed in a single peak curve; the peak of shoot dry matter appeared at 270 kg N/hm 2 . The N concentration and N content in shoot and root increased rapidly when the N rate changed from 90 to 180 kg/hm 2 . Moreover, the N concentration and N content root of in ZYZ12 were much higher than that of ZY116. Present study revealed that the changed trend of leaf nitrate reductase activities （NRA） were significantly increased at the N rate of 180 kg/hm 2 in ZY116 and ZYZ12 compared with the N rate of 90 kg/hm 2 in two years. [Conclusion] Optimal nitrogen application significantly increased the dry weights and N uptake at wintering stage as well as increasing the yield of winter oilseed rape.
文摘Nitrogen(N)assimilation is a wide pathway in plants because of its fundamental importance for growth and development.The transport,assimilation and recycling of nitrogen is a highly complex and regulated process,as it is the mineral nutrient that is required in great abundance by the plants.Basic approach to enhance agriculture sustainability is dependent on exploration of the elite germplasm where new cultivars could perform better even under low N.To test the effect of nitrogen levels at 100 and 125 kg ha-1 on photosynthetic pigments and N assimilation in oilseed rape(B.napus L.)canola variety GSC-7 and hybrid Hyola PAC 401 were selected for comparison with new hybrid PGSH-52 in pipeline.N assimilating enzymes were assayed at vegetative,flowering and siliquing stages of crop growth to visualise the impact of N on the productivity.Nitrogen assimilating enzymes were highest at flowering stage and enhanced with increased N level.Nitrate reductase(NR)activity improved by 11.5%,nitrite reductase(NiR)by 24.2%,glutamine synthetase(GS)by 12.2%and glutamate synthase(GOGAT)by 35.2%over recommended N dose(N100)at flowering stage.Hyola PAC 401 registered maximum enzymatic activities trailed by GSC-7 at all the three stages of crop growth.Differences existed within the genotypes for photosynthetic pigments which varied with N levels.Chlorophyll a,chlorophyll b,total chlorophyll and carotenoids increased with nitrogen at 125 kg N ha^(-1)whereas chlorophyll a/b declined at three stages of crop growth.At flowering total chlorophyll and carotenoids were maximum and enhanced by 12.8%and 5%respectively with higher nitrogen level.
