Objective:To investigate the clinical symptoms of coronavirus disease 2019(COVID-19)patients with and without B.1.1.7 mutation.Methods:This retrospective observational study included COVID-19 patients who were divided...Objective:To investigate the clinical symptoms of coronavirus disease 2019(COVID-19)patients with and without B.1.1.7 mutation.Methods:This retrospective observational study included COVID-19 patients who were divided into two groups,the mutation and the non-mutation group.Demographics characteristics,clinical characteristics,laboratory parameters,and mortality rates were recorded and compared between the two groups.Results:A total of 196 patients were included in the study.The relationship between the mutant virus status and sex,age,comorbidity,survival status,and disease severity was not significant(P>0.05).No significant differences were found in duration of hospitalization between the mutation and the non-mutation group(P>0.05).However,there was a statistically significant difference between patients with and without mutant viruses in hemoglobin,mean platelet volume,procalcitonin,low density lipoprotein,iron-binding capacity,potassium,calcium,C-reactive protein,folate,creatine kinase myocardial band,D-dimer,and international normalized ratio(P<0.05).Conclusions:No significant difference is found in mortality rate,disease severity or duration of hospitalization between the patients with and without variant B.1.1.7.Careful monitoring of COVID-19 patients is required for all variants.展开更多
Background:With the ongoing worldwide coronavirus disease 2019(COVID-19)pandemic,an increasing number of viral variants are being identified,which poses a challenge for nucleic acid-based diagnostic tests.Rapid tests,...Background:With the ongoing worldwide coronavirus disease 2019(COVID-19)pandemic,an increasing number of viral variants are being identified,which poses a challenge for nucleic acid-based diagnostic tests.Rapid tests,such as real-time reverse transcription-polymerase chain reaction(rRT-PCR),play an important role in monitoring COVID-19 infection and controlling its spread.However,the changes in the genotypes of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants may result in decreased sensitivity of the rRT-PCR assay and it is necessary to monitor the mutations in primers and probes of SARSCoV-2 detection over time.Methods:We developed two rRT-PCR assays to detect the RNA-dependent RNA polymerase(RdRp)and nucleocapsid(N)genes of SARS-CoV-2.We evaluated these assays together with our previously published assays targeting the ORF1ab and N genes for the detection and confirmation of SARS-CoV-2 and its variants of concern(VOCs).In addition,we also developed two rRT-PCR assays(S484K and S501Y)targeting the spike gene,which when combined with the open reading frames(ORF)1ab assay,respectively,to form duplex rRT-PCR assays,were able to detect SARS-CoV-2 VOCs(lineages B.1.351 and B.1.1.7).Results:Using a SARS-CoV-2 stock with predetermined genomic copies as a standard,the detection limit of both assays targeting RdRp and N was five copies/reaction.Furthermore,no cross-reactions with six others human CoVs(229E,OC43,NL63,HKU1,severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus)were observed using these assays.In addition,the S484K and S501Y assays were combined with the ORF1ab assay,respectively.Conclusions:Four rRT-PCR assays(RdRp,N,S484K,and S501Y)were used to detect SARS-CoV-2 variants,and these assays were shown to be effective in screening for multiple virus strains.展开更多
文摘Objective:To investigate the clinical symptoms of coronavirus disease 2019(COVID-19)patients with and without B.1.1.7 mutation.Methods:This retrospective observational study included COVID-19 patients who were divided into two groups,the mutation and the non-mutation group.Demographics characteristics,clinical characteristics,laboratory parameters,and mortality rates were recorded and compared between the two groups.Results:A total of 196 patients were included in the study.The relationship between the mutant virus status and sex,age,comorbidity,survival status,and disease severity was not significant(P>0.05).No significant differences were found in duration of hospitalization between the mutation and the non-mutation group(P>0.05).However,there was a statistically significant difference between patients with and without mutant viruses in hemoglobin,mean platelet volume,procalcitonin,low density lipoprotein,iron-binding capacity,potassium,calcium,C-reactive protein,folate,creatine kinase myocardial band,D-dimer,and international normalized ratio(P<0.05).Conclusions:No significant difference is found in mortality rate,disease severity or duration of hospitalization between the patients with and without variant B.1.1.7.Careful monitoring of COVID-19 patients is required for all variants.
基金This work was supported by grants from the National Key Research and Development Program of China(Nos.2016YFD0500301,2021YFC0863300,and 2020YFC0840900).
文摘Background:With the ongoing worldwide coronavirus disease 2019(COVID-19)pandemic,an increasing number of viral variants are being identified,which poses a challenge for nucleic acid-based diagnostic tests.Rapid tests,such as real-time reverse transcription-polymerase chain reaction(rRT-PCR),play an important role in monitoring COVID-19 infection and controlling its spread.However,the changes in the genotypes of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants may result in decreased sensitivity of the rRT-PCR assay and it is necessary to monitor the mutations in primers and probes of SARSCoV-2 detection over time.Methods:We developed two rRT-PCR assays to detect the RNA-dependent RNA polymerase(RdRp)and nucleocapsid(N)genes of SARS-CoV-2.We evaluated these assays together with our previously published assays targeting the ORF1ab and N genes for the detection and confirmation of SARS-CoV-2 and its variants of concern(VOCs).In addition,we also developed two rRT-PCR assays(S484K and S501Y)targeting the spike gene,which when combined with the open reading frames(ORF)1ab assay,respectively,to form duplex rRT-PCR assays,were able to detect SARS-CoV-2 VOCs(lineages B.1.351 and B.1.1.7).Results:Using a SARS-CoV-2 stock with predetermined genomic copies as a standard,the detection limit of both assays targeting RdRp and N was five copies/reaction.Furthermore,no cross-reactions with six others human CoVs(229E,OC43,NL63,HKU1,severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus)were observed using these assays.In addition,the S484K and S501Y assays were combined with the ORF1ab assay,respectively.Conclusions:Four rRT-PCR assays(RdRp,N,S484K,and S501Y)were used to detect SARS-CoV-2 variants,and these assays were shown to be effective in screening for multiple virus strains.
