背景:目前发现miR-196b-5p在细胞增殖、迁移及抑制瘢痕增生中发挥作用,但在创面愈合过程中是否发挥作用缺乏相关研究。目的:探讨脂肪干细胞源性外泌体中miR-196b-5p对烧伤创面愈合的影响。方法:构建SD大鼠皮肤深Ⅱ度烧伤模型,随机分为4...背景:目前发现miR-196b-5p在细胞增殖、迁移及抑制瘢痕增生中发挥作用,但在创面愈合过程中是否发挥作用缺乏相关研究。目的:探讨脂肪干细胞源性外泌体中miR-196b-5p对烧伤创面愈合的影响。方法:构建SD大鼠皮肤深Ⅱ度烧伤模型,随机分为4组:空白对照组、外泌体组、agomiR-196b-5p组和外泌体+antagomiR-196b-5p组,每组10只,根据不同分组于创周注射PBS、脂肪干细胞源性外泌体、miR-196b-5p激动剂和miR-196b-5p抑制剂,伤后即刻和伤后7,14,21 d观察创面愈合情况,伤后7 d苏木精-伊红染色观察创面炎症表达,伤后14 d Masson染色观察创面胶原表达及免疫组化染色观察创面CD31表达,伤后7 d Western blot检测创面中α-平滑肌肌动蛋白、Ⅰ型胶原蛋白表达。结果与结论:①agomiR-196b-5p组创面愈合较快,空白对照组和外泌体+antagomiR-196b-5p组愈合较慢;②与空白对照组和外泌体+antagomiR-196b-5p组相比,外泌体组和agomiR-196b-5p组创面炎性细胞浸润较少,CD31表达明显增加(P<0.01);③与空白对照组和外泌体+antagomiR-196b-5p组相比,外泌体组和agomiR-196b-5p组中α-平滑肌肌动蛋白、Ⅰ型胶原蛋白表达升高(P<0.05)。结果表明,脂肪干细胞源性外泌体中miR-196b-5p能促进大鼠烧伤创面愈合。展开更多
In this paper,we study the generalized Dedekind Rademacher sums considered by Hall,Wilson and Zagier.We establish a new formula for the products of two Bernoulli functions by using Parseval's formula,Hurwitz's...In this paper,we study the generalized Dedekind Rademacher sums considered by Hall,Wilson and Zagier.We establish a new formula for the products of two Bernoulli functions by using Parseval's formula,Hurwitz's formula and Lerch's functional equation.As applications of the result,some well-known reciprocity formulas are deduced as special cases.展开更多
Polynomial splines have played an important role in image processing, medical imaging and wavelet theory. Exponential splines which are of more general concept have been recently investigated.We focus on cardinal expo...Polynomial splines have played an important role in image processing, medical imaging and wavelet theory. Exponential splines which are of more general concept have been recently investigated.We focus on cardinal exponential splines and develop a method to implement the exponential B-splines which form a Riesz basis of the space of cardinal exponential splines with finite energy.展开更多
文摘背景:目前发现miR-196b-5p在细胞增殖、迁移及抑制瘢痕增生中发挥作用,但在创面愈合过程中是否发挥作用缺乏相关研究。目的:探讨脂肪干细胞源性外泌体中miR-196b-5p对烧伤创面愈合的影响。方法:构建SD大鼠皮肤深Ⅱ度烧伤模型,随机分为4组:空白对照组、外泌体组、agomiR-196b-5p组和外泌体+antagomiR-196b-5p组,每组10只,根据不同分组于创周注射PBS、脂肪干细胞源性外泌体、miR-196b-5p激动剂和miR-196b-5p抑制剂,伤后即刻和伤后7,14,21 d观察创面愈合情况,伤后7 d苏木精-伊红染色观察创面炎症表达,伤后14 d Masson染色观察创面胶原表达及免疫组化染色观察创面CD31表达,伤后7 d Western blot检测创面中α-平滑肌肌动蛋白、Ⅰ型胶原蛋白表达。结果与结论:①agomiR-196b-5p组创面愈合较快,空白对照组和外泌体+antagomiR-196b-5p组愈合较慢;②与空白对照组和外泌体+antagomiR-196b-5p组相比,外泌体组和agomiR-196b-5p组创面炎性细胞浸润较少,CD31表达明显增加(P<0.01);③与空白对照组和外泌体+antagomiR-196b-5p组相比,外泌体组和agomiR-196b-5p组中α-平滑肌肌动蛋白、Ⅰ型胶原蛋白表达升高(P<0.05)。结果表明,脂肪干细胞源性外泌体中miR-196b-5p能促进大鼠烧伤创面愈合。
基金Supported by the Natural Science Foundation of Sichuan Province(No.2023NSFSC0065)。
文摘In this paper,we study the generalized Dedekind Rademacher sums considered by Hall,Wilson and Zagier.We establish a new formula for the products of two Bernoulli functions by using Parseval's formula,Hurwitz's formula and Lerch's functional equation.As applications of the result,some well-known reciprocity formulas are deduced as special cases.
文摘Polynomial splines have played an important role in image processing, medical imaging and wavelet theory. Exponential splines which are of more general concept have been recently investigated.We focus on cardinal exponential splines and develop a method to implement the exponential B-splines which form a Riesz basis of the space of cardinal exponential splines with finite energy.
