In the early stages of traumatic spinal cord injury,extensive accumulation of autophagosomes creates a neurotoxic microenvironment,exacerbating neuronal cell death and worsening tissue damage,ultimately hindering neur...In the early stages of traumatic spinal cord injury,extensive accumulation of autophagosomes creates a neurotoxic microenvironment,exacerbating neuronal cell death and worsening tissue damage,ultimately hindering neurofunctional recovery.Activin A is a critical growth factor necessary for the development of the embryonic nervous system and for maintaining neuronal function in the adult cerebral cortex.It can inhibit excessive autophagy in ischemic stroke to reduce neuronal damage.However,the specific mechanism through which Activin A functions in the spinal cord remains poorly understood.In this study,we administered different concentrations of Activin A to neural stem cells from the spinal cord and found that Activin A stimulated the proliferation and neuronal differentiation of neural stem cells.Then,we established an in vitro oxidative stress model by using hydrogen peroxide to stimulate the neural stem cells-induced neurons.We found that Activin A could reduce apoptosis caused by oxidative stress.Subsequently,we treated a mouse model of spinal cord contusion with intrathecal injection of Activin A.Behavioral and electrophysiological results showed that Activin A promoted recovery of motor function and reconstruction of neural circuits in the model mice.Finally,RNA sequencing indicated that Activin A inhibited autophagy by activating the PI3K/AKT/mTOR pathway and upregulating the expression of synaptogenesis-related factor Sema3A in the spinal cord.These results suggest that Activin A may mediate the excessive autophagic response after spinal cord injury,promote the reconstruction of damaged neural circuits,and restore neurological function in the injured spinal cord.展开更多
Based on the superiority of adaptive filtering algorithms designed with hyperbolic function-like objective functions,this paper proposes generalized spline adaptive filtering(SAF)algorithms designed with hyperbolic fu...Based on the superiority of adaptive filtering algorithms designed with hyperbolic function-like objective functions,this paper proposes generalized spline adaptive filtering(SAF)algorithms designed with hyperbolic function-like objective functions.Specifically,a series of generalized new SAF algorithms are proposed by introducing the q-deformed hyperbolic function as the cost function,named SAF-qDHSI,SAF-qDHCO,SAFqDHTA&SAF-qDHSE algorithms,respectively.Then,the proposed algorithm is theoretically demonstrated with detailed mean convergence and computational complexity analysis;secondly,the effect of different q values on the performance of the new algorithm is verified through data simulation;the new algorithm still has better performance under the interference of Gaussian noise and non-Gaussian noise even when facing the system mutation;finally,the new algorithm is verified through the measured engineering data,and the results show that the new algorithm has better convergence and robustness compared with the existing algorithm.In conclusion,the generalized algorithm based on the new cost function proposed in this paper is more effective in nonlinear system identification.展开更多
The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury.Regulation of shifting microglia ...The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury.Regulation of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be crucial.Tryptanthrin possesses an anti-inflammatory biological function.However,its roles and the underlying molecular mechanisms in spinal cord injury remain unknown.In this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in vitro.Tryptanthrin promoted M2 polarization through inactivating the cGAS/STING/NF-κB pathway.Additionally,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord injury.Finally,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal apoptosis.Taken together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.展开更多
Modelling and simulation of projectile flight is at the core of ballistic computer software and is essential to the study of performance of rifles and projectiles in various engagement conditions.An effective and repr...Modelling and simulation of projectile flight is at the core of ballistic computer software and is essential to the study of performance of rifles and projectiles in various engagement conditions.An effective and representative numerical model of projectile flight requires a relatively good approximation of the aerodynamics.The aerodynamic coefficients of the projectile model should be described as a series of piecewise polynomial functions of the Mach number that ideally meet the following conditions:they are continuous,differentiable at least once,and have a relatively low degree.The paper provides the steps needed to generate such piecewise polynomial functions using readily available tools,and then compares Piecewise Cubic Hermite Interpolating Polynomial(PCHIP),cubic splines,and piecewise linear functions,and their variant,as potential curve fitting methods to approximate the aerodynamics of a generic small arms projectile.A key contribution of the paper is the application of PCHIP to the approximation of projectile aerodynamics,and its evaluation against a set of criteria.Finally,the paper provides a baseline assessment of the impact of the polynomial functions on flight trajectory predictions obtained with 6-degree-of-freedom simulations of a generic projectile.展开更多
This paper describes an approximating solution, based on Lagrange interpolation and spline functions, to treat functional integral equations of Fredholm type and Volterra type. This method extended to functional integ...This paper describes an approximating solution, based on Lagrange interpolation and spline functions, to treat functional integral equations of Fredholm type and Volterra type. This method extended to functional integral and integro-differential equations. For showing efficiency of the method we give some numerical examples.展开更多
Chronic hepatitis B(CHB)remains a significant global health challenge,affecting more than 250 million individuals worldwide.A functional cure,defined as the loss of hepatitis B surface antigen(HBsAg)and suppression of...Chronic hepatitis B(CHB)remains a significant global health challenge,affecting more than 250 million individuals worldwide.A functional cure,defined as the loss of hepatitis B surface antigen(HBsAg)and suppression of hepatitis B virus(HBV)DNA to undetectable levels,represents the optimal therapeutic endpoint for managing CHB.However,the complex pathogenesis of CHB,which includes HBV DNA integration,persistence of covalently closed circular DNA,and impaired immune responses,presents substantial barriers to HBsAg clearance.Current therapies offer limited success in achieving a functional cure,with HBsAg seroclearance occurring in only 3%-5%of patients after 10 years of nucleos(t)ide analogs(NAs)therapy and 8%-14%within 3-5 years of pegylated interferon treatment.To overcome these limitations,novel direct-acting antivirals targeting different stages of the HBV life cycle are being investigated.Additionally,immunomodulatory approaches,including therapeutic vaccines and immune checkpoint inhibitors,are being explored to enhance HBV-specific immune responses.The concept of NAs cessation in carefully selected non-cirrhotic patients may accelerate HBsAg loss,although the risks of hepatic flare and hepatocellular carcinoma necessitate rigorous monitoring.This review provides a comprehensive overview of the current understanding of HBsAg seroclearance in CHB,discussing its clinical significance,therapeutic challenges,and evolving treatment landscape in the pursuit of a functional cure.展开更多
According to the B-spline convolution mask, first, the contrast sensitiveness （CS） is computed and then is viewed as a noise sensitiveness coeficient （NSC） to adaptively determine a noise-recognized threshold valu...According to the B-spline convolution mask, first, the contrast sensitiveness （CS） is computed and then is viewed as a noise sensitiveness coeficient （NSC） to adaptively determine a noise-recognized threshold value. Based on the noise density function （NDF） in a 3×3 window, the filtering window size is adaptively adjusted, and then a median filter is used to eliminate the noise-marked pixels. The experiment results show that the proposed algorithm can preserve image detail information well and effectively remove the noises, particularly the impulse noises that is also called salt-and-pepper noises superimposed on the computed tomography （CT） and magnetic resonance imaging （MRI） medical images.展开更多
In this article, we develop numerical method by constructing ninth degree spline function using extended cubic spline Bickley’s method to find the approximate solution of seventh order linear boundary value problems ...In this article, we develop numerical method by constructing ninth degree spline function using extended cubic spline Bickley’s method to find the approximate solution of seventh order linear boundary value problems at different step lengths. The approximate solution is compared with the solution obtained by eighth degree splines and exact solution. It has been observed that the approximate solution is an excellent agreement with exact solution. Low absolute error indicates that our numerical method is effective for solving high order linear boundary value problems.展开更多
A new method——the third power B-spline function method is developed to analyse the stability and the buckle of rolled strip under residual stress.The large deflection theory of thin plate is used to calculate the bu...A new method——the third power B-spline function method is developed to analyse the stability and the buckle of rolled strip under residual stress.The large deflection theory of thin plate is used to calculate the buckle of rolled strip and criterion of critical buckle is given.The computed results tally with those of experiment well,which provides theoretical basis and method for developing the mathematical model of flatness control.展开更多
In this paper,we give four characteristic theorems of the natural Tchebysheff splint functionassociated with multiple knots.These theorems possess specific form,that arc convenient forapplicaton;In the case of with si...In this paper,we give four characteristic theorems of the natural Tchebysheff splint functionassociated with multiple knots.These theorems possess specific form,that arc convenient forapplicaton;In the case of with simple knots or polynomial splint,the corollaries of this paper’s the-orems give corresponding results.展开更多
Ocean remote sensing satellites provide observations with high spatiotemporal resolution.However,the influence of clouds,fog,and haze frequently leads to significant data gaps.Accurate and effective estimation of thes...Ocean remote sensing satellites provide observations with high spatiotemporal resolution.However,the influence of clouds,fog,and haze frequently leads to significant data gaps.Accurate and effective estimation of these missing data is highly valuable for engineering and scientific research.In this study,the radial basis function(RBF)method is used to estimate the spatial distribution of total suspended matter(TSM)concentration in Hangzhou Bay using remote sensing data with severe data gaps.The estimation precision is validated by comparing the results with those of other commonly used interpolation methods,such as the Kriging method and the basic spline(B-spline)method.In addition,the applicability of the RBF method is explored.Results show that the estimation of the RBF method is significantly close to the observation in Hangzhou Bay.The average of the mean absolute error,mean relative error,and root mean square error in all the experiments is evidently smaller than those of the Kriging and B-spline interpolations,indicating that the proposed method is more appropriate for estimating the spatial distribution of the TSM in Hangzhou Bay.Finally,the TSM distribution in the blank observational area is predicted.This study can provide some reference values for handling watercolor remote sensing data.展开更多
BACKGROUND The relationship between hepatitis B surface antigen(HBsAg)concentrations,hepatitis B virus(HBV)DNA levels,and hepatic function in individuals with chronic hepatitis B(CHB)remains incompletely characterized...BACKGROUND The relationship between hepatitis B surface antigen(HBsAg)concentrations,hepatitis B virus(HBV)DNA levels,and hepatic function in individuals with chronic hepatitis B(CHB)remains incompletely characterized.AIM To examine the association of serum HBsAg concentrations with HBV DNA levels and hepatic function parameters in patients with CHB.METHODS A total of 110 individuals with CHB admitted to Kunming Third People’s Hospital between January 2023 and January 2025 were enrolled as the observation group,whereas 70 age-and sex-matched healthy individuals served as the control group.Fasting peripheral venous blood(5 mL)was collected from all participants.Serum HBsAg and HBV DNA levels(in the observation group),along with hepatic function markers,including total bilirubin(TBIL),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were measured in both groups.Pearson correlation analysis was used to assess the association between serum HBsAg levels and HBV DNA,TBIL,AST,and ALT levels in patients with CHB.Receiver operating characteristic(ROC)curve analysis was conducted to determine optimal cutoff values of HBsAg for predicting high viral load(HBV DNA≥10^(5) IU/mL)and significant liver injury(ALT≥2×upper limit of normal[ULN]).RESULTS HBsAg levels differed significantly across CHB phases:Immune tolerance(IT)phase(4.62±1.51 lgIU/mL),immune clearance(IC)phase(3.84±1.16 lgIU/mL),low replication(LR)phase(2.99±0.66 lgIU/mL),and HBV e antigen-negative hepatitis(ENH)phase(3.40±0.69 lgIU/mL).Corresponding HBV DNA levels were highest in the IT phase(7.41±1.83 log copies/mL),followed by the IC phase(6.03±1.92 log copies/mL),ENH phase(3.89±1.23 log copies/mL),and LR phase(2.55±1.00 log copies/mL).All hepatic function parameters in patients with CHB were significantly elevated compared to the healthy controls.Pearson correlation analysis showed significant positive associations between serum HBsAg levels and HBV DNA,TBIL,AST,and ALT levels.ROC analysis revealed that an HBsAg cutoff>4.09 lgIU/mL predicted HBV DNA≥105 IU/mL(high viral load)with 88.57%sensitivity,78.67%specificity,and an area under the curve(AUC)of 0.868(P<0.001),while a cutoff>4.07 lgIU/mL predicted ALT≥2×ULN(significant liver injury)with 69.70%sensitivity,90.91%specificity,and an AUC of 0.821(P<0.001).CONCLUSION Serum HBsAg,a noninvasive serological marker,holds significant clinical value in CHB management by aiding in the stratification of viral burden and the prediction of hepatic impairment.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82271419,81901902(to YZ),82202702(to ZW),82202351(to XH),82301550(to LYang),82271418(to XX)the Shanghai Rising-Star Program,No.22QA1408200(to YZ)the Fundamental Research Fundsfor the Central Universities,No.22120220555(to YZ).
文摘In the early stages of traumatic spinal cord injury,extensive accumulation of autophagosomes creates a neurotoxic microenvironment,exacerbating neuronal cell death and worsening tissue damage,ultimately hindering neurofunctional recovery.Activin A is a critical growth factor necessary for the development of the embryonic nervous system and for maintaining neuronal function in the adult cerebral cortex.It can inhibit excessive autophagy in ischemic stroke to reduce neuronal damage.However,the specific mechanism through which Activin A functions in the spinal cord remains poorly understood.In this study,we administered different concentrations of Activin A to neural stem cells from the spinal cord and found that Activin A stimulated the proliferation and neuronal differentiation of neural stem cells.Then,we established an in vitro oxidative stress model by using hydrogen peroxide to stimulate the neural stem cells-induced neurons.We found that Activin A could reduce apoptosis caused by oxidative stress.Subsequently,we treated a mouse model of spinal cord contusion with intrathecal injection of Activin A.Behavioral and electrophysiological results showed that Activin A promoted recovery of motor function and reconstruction of neural circuits in the model mice.Finally,RNA sequencing indicated that Activin A inhibited autophagy by activating the PI3K/AKT/mTOR pathway and upregulating the expression of synaptogenesis-related factor Sema3A in the spinal cord.These results suggest that Activin A may mediate the excessive autophagic response after spinal cord injury,promote the reconstruction of damaged neural circuits,and restore neurological function in the injured spinal cord.
基金financially supported by the National Natural Science Foundation of China (8225041038)the Sichuan Science and Technology Program (23NSFSC2916)the Fundamental Research Funds for the Central Universities, Southwest Minzu University (ZYN2024077)
文摘Based on the superiority of adaptive filtering algorithms designed with hyperbolic function-like objective functions,this paper proposes generalized spline adaptive filtering(SAF)algorithms designed with hyperbolic function-like objective functions.Specifically,a series of generalized new SAF algorithms are proposed by introducing the q-deformed hyperbolic function as the cost function,named SAF-qDHSI,SAF-qDHCO,SAFqDHTA&SAF-qDHSE algorithms,respectively.Then,the proposed algorithm is theoretically demonstrated with detailed mean convergence and computational complexity analysis;secondly,the effect of different q values on the performance of the new algorithm is verified through data simulation;the new algorithm still has better performance under the interference of Gaussian noise and non-Gaussian noise even when facing the system mutation;finally,the new algorithm is verified through the measured engineering data,and the results show that the new algorithm has better convergence and robustness compared with the existing algorithm.In conclusion,the generalized algorithm based on the new cost function proposed in this paper is more effective in nonlinear system identification.
基金supported by the National Natural Science Foundation of China,Nos.82071387(to HT),81971172(to YW)the Natural Science Foundation of Zhejiang Province,China,No.LY22H090012(to HT)the Basic Research Project of Wenzhou City,China,No.Y20220923(to MZ)。
文摘The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord injury.Regulation of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be crucial.Tryptanthrin possesses an anti-inflammatory biological function.However,its roles and the underlying molecular mechanisms in spinal cord injury remain unknown.In this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in vitro.Tryptanthrin promoted M2 polarization through inactivating the cGAS/STING/NF-κB pathway.Additionally,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord injury.Finally,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal apoptosis.Taken together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.
文摘Modelling and simulation of projectile flight is at the core of ballistic computer software and is essential to the study of performance of rifles and projectiles in various engagement conditions.An effective and representative numerical model of projectile flight requires a relatively good approximation of the aerodynamics.The aerodynamic coefficients of the projectile model should be described as a series of piecewise polynomial functions of the Mach number that ideally meet the following conditions:they are continuous,differentiable at least once,and have a relatively low degree.The paper provides the steps needed to generate such piecewise polynomial functions using readily available tools,and then compares Piecewise Cubic Hermite Interpolating Polynomial(PCHIP),cubic splines,and piecewise linear functions,and their variant,as potential curve fitting methods to approximate the aerodynamics of a generic small arms projectile.A key contribution of the paper is the application of PCHIP to the approximation of projectile aerodynamics,and its evaluation against a set of criteria.Finally,the paper provides a baseline assessment of the impact of the polynomial functions on flight trajectory predictions obtained with 6-degree-of-freedom simulations of a generic projectile.
文摘This paper describes an approximating solution, based on Lagrange interpolation and spline functions, to treat functional integral equations of Fredholm type and Volterra type. This method extended to functional integral and integro-differential equations. For showing efficiency of the method we give some numerical examples.
文摘Chronic hepatitis B(CHB)remains a significant global health challenge,affecting more than 250 million individuals worldwide.A functional cure,defined as the loss of hepatitis B surface antigen(HBsAg)and suppression of hepatitis B virus(HBV)DNA to undetectable levels,represents the optimal therapeutic endpoint for managing CHB.However,the complex pathogenesis of CHB,which includes HBV DNA integration,persistence of covalently closed circular DNA,and impaired immune responses,presents substantial barriers to HBsAg clearance.Current therapies offer limited success in achieving a functional cure,with HBsAg seroclearance occurring in only 3%-5%of patients after 10 years of nucleos(t)ide analogs(NAs)therapy and 8%-14%within 3-5 years of pegylated interferon treatment.To overcome these limitations,novel direct-acting antivirals targeting different stages of the HBV life cycle are being investigated.Additionally,immunomodulatory approaches,including therapeutic vaccines and immune checkpoint inhibitors,are being explored to enhance HBV-specific immune responses.The concept of NAs cessation in carefully selected non-cirrhotic patients may accelerate HBsAg loss,although the risks of hepatic flare and hepatocellular carcinoma necessitate rigorous monitoring.This review provides a comprehensive overview of the current understanding of HBsAg seroclearance in CHB,discussing its clinical significance,therapeutic challenges,and evolving treatment landscape in the pursuit of a functional cure.
基金supported by Foundation of 11th Five-year Plan for Key Construction Academic Subject (Optics) of Hunan Province,PRC, Outstanding Young Scientific Research Fund of Hunan Provincial Education Department, PRC (No. 09B071)Scientific Research Fund of Hunan Provincial Education Department, PRC(No. 06C581)
文摘According to the B-spline convolution mask, first, the contrast sensitiveness （CS） is computed and then is viewed as a noise sensitiveness coeficient （NSC） to adaptively determine a noise-recognized threshold value. Based on the noise density function （NDF） in a 3×3 window, the filtering window size is adaptively adjusted, and then a median filter is used to eliminate the noise-marked pixels. The experiment results show that the proposed algorithm can preserve image detail information well and effectively remove the noises, particularly the impulse noises that is also called salt-and-pepper noises superimposed on the computed tomography （CT） and magnetic resonance imaging （MRI） medical images.
文摘In this article, we develop numerical method by constructing ninth degree spline function using extended cubic spline Bickley’s method to find the approximate solution of seventh order linear boundary value problems at different step lengths. The approximate solution is compared with the solution obtained by eighth degree splines and exact solution. It has been observed that the approximate solution is an excellent agreement with exact solution. Low absolute error indicates that our numerical method is effective for solving high order linear boundary value problems.
文摘A new method——the third power B-spline function method is developed to analyse the stability and the buckle of rolled strip under residual stress.The large deflection theory of thin plate is used to calculate the buckle of rolled strip and criterion of critical buckle is given.The computed results tally with those of experiment well,which provides theoretical basis and method for developing the mathematical model of flatness control.
文摘In this paper,we give four characteristic theorems of the natural Tchebysheff splint functionassociated with multiple knots.These theorems possess specific form,that arc convenient forapplicaton;In the case of with simple knots or polynomial splint,the corollaries of this paper’s the-orems give corresponding results.
基金supported by the Open Funds for Hubei Key Laboratory of Marine Geological Resources,China University of Geosciences(No.MGR202308)the Natural Science Foundation of Shandong Province(No.ZR2020MD085)+3 种基金the National Natural Science Foundation of China(No.41821004)the Taishan Scholar Program(No.tstp2022114)the Shandong Provincial Natural Science Foundation(No.DKXZZ202206)the National Key Research and Development Program of China(No.2016YFC1402404).
文摘Ocean remote sensing satellites provide observations with high spatiotemporal resolution.However,the influence of clouds,fog,and haze frequently leads to significant data gaps.Accurate and effective estimation of these missing data is highly valuable for engineering and scientific research.In this study,the radial basis function(RBF)method is used to estimate the spatial distribution of total suspended matter(TSM)concentration in Hangzhou Bay using remote sensing data with severe data gaps.The estimation precision is validated by comparing the results with those of other commonly used interpolation methods,such as the Kriging method and the basic spline(B-spline)method.In addition,the applicability of the RBF method is explored.Results show that the estimation of the RBF method is significantly close to the observation in Hangzhou Bay.The average of the mean absolute error,mean relative error,and root mean square error in all the experiments is evidently smaller than those of the Kriging and B-spline interpolations,indicating that the proposed method is more appropriate for estimating the spatial distribution of the TSM in Hangzhou Bay.Finally,the TSM distribution in the blank observational area is predicted.This study can provide some reference values for handling watercolor remote sensing data.
文摘BACKGROUND The relationship between hepatitis B surface antigen(HBsAg)concentrations,hepatitis B virus(HBV)DNA levels,and hepatic function in individuals with chronic hepatitis B(CHB)remains incompletely characterized.AIM To examine the association of serum HBsAg concentrations with HBV DNA levels and hepatic function parameters in patients with CHB.METHODS A total of 110 individuals with CHB admitted to Kunming Third People’s Hospital between January 2023 and January 2025 were enrolled as the observation group,whereas 70 age-and sex-matched healthy individuals served as the control group.Fasting peripheral venous blood(5 mL)was collected from all participants.Serum HBsAg and HBV DNA levels(in the observation group),along with hepatic function markers,including total bilirubin(TBIL),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were measured in both groups.Pearson correlation analysis was used to assess the association between serum HBsAg levels and HBV DNA,TBIL,AST,and ALT levels in patients with CHB.Receiver operating characteristic(ROC)curve analysis was conducted to determine optimal cutoff values of HBsAg for predicting high viral load(HBV DNA≥10^(5) IU/mL)and significant liver injury(ALT≥2×upper limit of normal[ULN]).RESULTS HBsAg levels differed significantly across CHB phases:Immune tolerance(IT)phase(4.62±1.51 lgIU/mL),immune clearance(IC)phase(3.84±1.16 lgIU/mL),low replication(LR)phase(2.99±0.66 lgIU/mL),and HBV e antigen-negative hepatitis(ENH)phase(3.40±0.69 lgIU/mL).Corresponding HBV DNA levels were highest in the IT phase(7.41±1.83 log copies/mL),followed by the IC phase(6.03±1.92 log copies/mL),ENH phase(3.89±1.23 log copies/mL),and LR phase(2.55±1.00 log copies/mL).All hepatic function parameters in patients with CHB were significantly elevated compared to the healthy controls.Pearson correlation analysis showed significant positive associations between serum HBsAg levels and HBV DNA,TBIL,AST,and ALT levels.ROC analysis revealed that an HBsAg cutoff>4.09 lgIU/mL predicted HBV DNA≥105 IU/mL(high viral load)with 88.57%sensitivity,78.67%specificity,and an area under the curve(AUC)of 0.868(P<0.001),while a cutoff>4.07 lgIU/mL predicted ALT≥2×ULN(significant liver injury)with 69.70%sensitivity,90.91%specificity,and an AUC of 0.821(P<0.001).CONCLUSION Serum HBsAg,a noninvasive serological marker,holds significant clinical value in CHB management by aiding in the stratification of viral burden and the prediction of hepatic impairment.
