Insulin resistance is the pathophysiological basis of many diseases.Overcoming early insulin resistance highly significant in prevention diabetes,non-alcoholic fatty liver,and atherosclerosis.The present study aimed a...Insulin resistance is the pathophysiological basis of many diseases.Overcoming early insulin resistance highly significant in prevention diabetes,non-alcoholic fatty liver,and atherosclerosis.The present study aimed at evaluating the therapeutic effects of baicalin on insulin resistance and skeletal muscle ectopic fat storage in high fat diet-induced mice,and exploring the potential molecular mechanisms.Insulin resistance in mice was induced with a high fat diet for 16 weeks.Animals were then treated with three different doses of baicalin(100,200,and 400 mg·kg^(-1)·d^(-1)for 14 weeks.Fasting blood glucose,fasting serum insulin,glucose tolerance test(GTT),insulin tolerance test(ITT),and skeletal muscle lipid deposition were measured.Additionally,the AMP-activated protein kinase/acetyl-CoA carboxylase and protein kinase B/Glycogen synthase kinase 3 beta pathways in skeletal muscle were further evaluated.Baicalin significantly reduced the levels of fasting blood glucose and fasting serum insulin and attenuated high fat diet induced glucose tolerance and insulin tolerance.Moreover,insulin resistance was significantly reversed.Pathological analysis revealed baicalin dose-dependently decreased the degree of the ectopic fat storage in skeletal muscle.The properties of baicalin were mediated,at least in part,by inhibition of the AMPK/ACC pathway,a key regulator of de novo lipogenesis and activation of the Akt/GSK-3β pathway,a key regulator of Glycogen synthesis.These data suggest that baicalin,at dose up to 400 mg·kg^(-1)·d^(-1),is safe and able to attenuate insulin resistance and skeletal muscle ectopic fat storage,through modulating the skeletal muscle AMPK/ACC pathway and Akt/GSK-3β pathway.展开更多
弥漫大B细胞淋巴瘤(diffuse large B cell lym-phoma,DLBCL)和多发性骨髓瘤(multiple myeloma,MM)均为常见的血液学肿瘤性疾病。DLBCL是一种起源于B细胞弥漫增生为主的恶性侵袭性肿瘤,是非霍奇金淋巴瘤中成年人群最常见的一种;MM是一种...弥漫大B细胞淋巴瘤(diffuse large B cell lym-phoma,DLBCL)和多发性骨髓瘤(multiple myeloma,MM)均为常见的血液学肿瘤性疾病。DLBCL是一种起源于B细胞弥漫增生为主的恶性侵袭性肿瘤,是非霍奇金淋巴瘤中成年人群最常见的一种;MM是一种起源于浆细胞增殖异常的恶性肿瘤,多发生于老年人群。本院收治一例82岁男性患者,该患者经腹腔包块穿刺病理确诊弥漫大B细胞淋巴瘤;血清免疫固定电泳发现IgG-λ型M蛋白,骨髓检查显示骨髓瘤细胞(原浆细胞型)比例为17.5%,活检结果证实为λ型浆细胞骨髓瘤,确诊为DLBCL合并MM,目前同一例患者合并此两种恶性肿瘤病情的相对较少,临床上容易漏诊误诊,耽误治疗时机,且DLBCL合并MM并无确切的治疗指南,本病例予mini-CHOP方案化疗,短期疗效有部分缓解,提示治疗方案对肿瘤有一定控制作用,但一个疗程结束后患者因个人原因转院,本次住院以“未愈”状态结束。展开更多
赖氨酸特异性去甲基化酶6B(lysine-specific demethylase 6B,KDM6B)是含Jumonji C结构域蛋白家族(Jumonji C domain-containing protein family,JmjC)中的一种重要表观遗传因子,不仅在细胞分化、炎症反应、组织稳态和神经性疾病中发挥...赖氨酸特异性去甲基化酶6B(lysine-specific demethylase 6B,KDM6B)是含Jumonji C结构域蛋白家族(Jumonji C domain-containing protein family,JmjC)中的一种重要表观遗传因子,不仅在细胞分化、炎症反应、组织稳态和神经性疾病中发挥表观遗传调控作用,还对巨噬细胞的功能、免疫反应等具有关键调控意义。作为JmjC家族中唯一能响应类Toll受体(Toll-like receptor,TLR)信号的成员,KDM6B可在TLR信号刺激下被激活从而发挥功能。研究发现,KDM6B可以通过调节巨噬细胞的极化、影响细胞因子的表达水平以及参与肿瘤微环境调控等方式影响巨噬细胞,因此,KDM6B在免疫反应、炎症反应以及肿瘤等病理生理过程中发挥重要作用。KDM6B作为关键的表观遗传因子对巨噬细胞的功能具有调控作用,包括调节巨噬细胞的极化、炎症反应以及促纤维化等,有望成为研究免疫、炎症及肿瘤等相关疾病的潜在靶点。展开更多
基金supported by a grant provided by Southeast University(No.9224007044)
文摘Insulin resistance is the pathophysiological basis of many diseases.Overcoming early insulin resistance highly significant in prevention diabetes,non-alcoholic fatty liver,and atherosclerosis.The present study aimed at evaluating the therapeutic effects of baicalin on insulin resistance and skeletal muscle ectopic fat storage in high fat diet-induced mice,and exploring the potential molecular mechanisms.Insulin resistance in mice was induced with a high fat diet for 16 weeks.Animals were then treated with three different doses of baicalin(100,200,and 400 mg·kg^(-1)·d^(-1)for 14 weeks.Fasting blood glucose,fasting serum insulin,glucose tolerance test(GTT),insulin tolerance test(ITT),and skeletal muscle lipid deposition were measured.Additionally,the AMP-activated protein kinase/acetyl-CoA carboxylase and protein kinase B/Glycogen synthase kinase 3 beta pathways in skeletal muscle were further evaluated.Baicalin significantly reduced the levels of fasting blood glucose and fasting serum insulin and attenuated high fat diet induced glucose tolerance and insulin tolerance.Moreover,insulin resistance was significantly reversed.Pathological analysis revealed baicalin dose-dependently decreased the degree of the ectopic fat storage in skeletal muscle.The properties of baicalin were mediated,at least in part,by inhibition of the AMPK/ACC pathway,a key regulator of de novo lipogenesis and activation of the Akt/GSK-3β pathway,a key regulator of Glycogen synthesis.These data suggest that baicalin,at dose up to 400 mg·kg^(-1)·d^(-1),is safe and able to attenuate insulin resistance and skeletal muscle ectopic fat storage,through modulating the skeletal muscle AMPK/ACC pathway and Akt/GSK-3β pathway.
文摘弥漫大B细胞淋巴瘤(diffuse large B cell lym-phoma,DLBCL)和多发性骨髓瘤(multiple myeloma,MM)均为常见的血液学肿瘤性疾病。DLBCL是一种起源于B细胞弥漫增生为主的恶性侵袭性肿瘤,是非霍奇金淋巴瘤中成年人群最常见的一种;MM是一种起源于浆细胞增殖异常的恶性肿瘤,多发生于老年人群。本院收治一例82岁男性患者,该患者经腹腔包块穿刺病理确诊弥漫大B细胞淋巴瘤;血清免疫固定电泳发现IgG-λ型M蛋白,骨髓检查显示骨髓瘤细胞(原浆细胞型)比例为17.5%,活检结果证实为λ型浆细胞骨髓瘤,确诊为DLBCL合并MM,目前同一例患者合并此两种恶性肿瘤病情的相对较少,临床上容易漏诊误诊,耽误治疗时机,且DLBCL合并MM并无确切的治疗指南,本病例予mini-CHOP方案化疗,短期疗效有部分缓解,提示治疗方案对肿瘤有一定控制作用,但一个疗程结束后患者因个人原因转院,本次住院以“未愈”状态结束。
文摘赖氨酸特异性去甲基化酶6B(lysine-specific demethylase 6B,KDM6B)是含Jumonji C结构域蛋白家族(Jumonji C domain-containing protein family,JmjC)中的一种重要表观遗传因子,不仅在细胞分化、炎症反应、组织稳态和神经性疾病中发挥表观遗传调控作用,还对巨噬细胞的功能、免疫反应等具有关键调控意义。作为JmjC家族中唯一能响应类Toll受体(Toll-like receptor,TLR)信号的成员,KDM6B可在TLR信号刺激下被激活从而发挥功能。研究发现,KDM6B可以通过调节巨噬细胞的极化、影响细胞因子的表达水平以及参与肿瘤微环境调控等方式影响巨噬细胞,因此,KDM6B在免疫反应、炎症反应以及肿瘤等病理生理过程中发挥重要作用。KDM6B作为关键的表观遗传因子对巨噬细胞的功能具有调控作用,包括调节巨噬细胞的极化、炎症反应以及促纤维化等,有望成为研究免疫、炎症及肿瘤等相关疾病的潜在靶点。
