Nine novel compounds,comprising seven tetrahydroanthraquinones(auxarthrolones A−G,1−7),aγ-butenolide glycoside(malfilamentoside E,26),and aγ-butenolide(auxarthrolide A,27),together with eighteen known compounds(8−25...Nine novel compounds,comprising seven tetrahydroanthraquinones(auxarthrolones A−G,1−7),aγ-butenolide glycoside(malfilamentoside E,26),and aγ-butenolide(auxarthrolide A,27),together with eighteen known compounds(8−25)were isolated from rice-based solid culture of Auxarthron umbrinum(A.umbrinum)DSM3193 using the one strain many compounds(OSMAC)approach.The structural elucidation of these compounds was accomplished through nuclear magnetic resonance(NMR),mass spectrometry(MS),and NMR calculation combined with DP4+analysis or MAEΔΔδparameter,while the absolute configurations of new compounds were established through single-crystal X-ray diffraction,electronic circular dichroism(ECD)spectroscopic data analysis and/or chemical derivatization.Austrocortilutein(10)and auxarthrol H(14)demonstrated moderate cytotoxicity against U87 and U251[half maximal inhibitory concentration(IC_(50))3.5−12.1μmol·L^(−1)].Additionally,auxarthrolone A(1),auxarthrol H(14),eupolyphagin B(15),and 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone(17)exhibited torsional effects on fibroblast proliferation challenges induced by oleic acid,thus demonstrating fibroblast proliferation-promoting activity.展开更多
基金supported by the National Key Research and Development Program of China(No.2018YFA0901900)the National Science Fund for Distinguished Young Scholars(No.82225042).
文摘Nine novel compounds,comprising seven tetrahydroanthraquinones(auxarthrolones A−G,1−7),aγ-butenolide glycoside(malfilamentoside E,26),and aγ-butenolide(auxarthrolide A,27),together with eighteen known compounds(8−25)were isolated from rice-based solid culture of Auxarthron umbrinum(A.umbrinum)DSM3193 using the one strain many compounds(OSMAC)approach.The structural elucidation of these compounds was accomplished through nuclear magnetic resonance(NMR),mass spectrometry(MS),and NMR calculation combined with DP4+analysis or MAEΔΔδparameter,while the absolute configurations of new compounds were established through single-crystal X-ray diffraction,electronic circular dichroism(ECD)spectroscopic data analysis and/or chemical derivatization.Austrocortilutein(10)and auxarthrol H(14)demonstrated moderate cytotoxicity against U87 and U251[half maximal inhibitory concentration(IC_(50))3.5−12.1μmol·L^(−1)].Additionally,auxarthrolone A(1),auxarthrol H(14),eupolyphagin B(15),and 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone(17)exhibited torsional effects on fibroblast proliferation challenges induced by oleic acid,thus demonstrating fibroblast proliferation-promoting activity.
