Background:In a previous study,we found that Atractylodes macrocephala and Paeoniae radix(AM-PR)was useful for the alleviation of functional constipation(FC).However,the precise mechanism underlying the compatibility ...Background:In a previous study,we found that Atractylodes macrocephala and Paeoniae radix(AM-PR)was useful for the alleviation of functional constipation(FC).However,the precise mechanism underlying the compatibility between AM and PR in the treatment of FC remains uncertain.This study aims to analyze the pharmacokinetic differences in the active ingredients in the blood of rat models with FC when applied individually and in combination with AM-PR.It also seeks to compare the changes in the content of the active ingredient when applied individually and in combination with in vitro AM-PR,further in-depth investigation into its material foundation in terms of pharmacokinetics,as well as the composition of the substance.Methods:Blood microdialysis samples were collected using microdialysis technology.These samples from rats with FC were compared after administration of AM,PR,and AM-PR.The concentration of the main active ingredients was determined using the Ultra Performance Liquid Chromatography-Tunable Ultraviolet(UPLC-TUV)method.The concentration of the main active ingredients of the decoction compatibility before and after combining AM-PR was also determined using the UPLC-TUV method.Results:Our findings reveal that upon combination,the time to maximum concentration(T_(max))of isochlorogenic acid A(ICA-A)and ataridolide Ⅱ(ATR-Ⅱ)T_(max)was prolonged,terminal elimination half-life(T_(1/2))was reduced,and maximum plasma concentrations(C_(max))increased.The T_(max)of ataridolide Ⅲ(ATR-Ⅲ)remained consistent,whereas its T_(1/2)and C_(max)were significantly reduced.Conversely,for peoniflorin(PAE),T_(max)occurred sooner,T_(1/2)was shortened,and C_(max)increased.The T_(max)for albiflorin(ALB)remained consistent,whereas T_(1/2)and C_(max)witnessed significant increases.The area under the moment curve(AUMC)(0-t)and AUMC(0-∞)of PAE,ALB,ICA-A,ATR-Ⅱ experienced an increase after AM-PR administration in rats,attributable to the heightened C_(max).In comparison to individual herb administration,the T_(max)of ALB was advanced in combination,the T_(max)of PAE remained unchanged,and the T_(max)of ICA-A and ART-Ⅱ was delayed,with an increased area under the concentration-time curve(AUC)(0-t),indicating enhanced C_(max)and bioavailability.Furthermore,the dissolution rates of PAE,ICA-A,and ATR-Ⅱ significantly improved after compatibility.Conclusions:This study partially clarifies the rationale and compatibility of AM-PR in treating FC and offers a new perspective on the pharmacokinetic interactions of AM-PR in FC treatment.展开更多
First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provi...First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.展开更多
Three eudesmanolide sesquiterpene-phenol hybrids,atramacronoids A-C(1-3),featuring an unusual6/6/5/5/6 skeleton furnished by forming an unexpected C-8-C-16 linkage,were obtained from the rhizomes of Atractylodes macro...Three eudesmanolide sesquiterpene-phenol hybrids,atramacronoids A-C(1-3),featuring an unusual6/6/5/5/6 skeleton furnished by forming an unexpected C-8-C-16 linkage,were obtained from the rhizomes of Atractylodes macrocephala.Their structures and absolute configurations were elucidated by spectroscopic data analysis,chemical calculations,combined with X-ray diffractions.The plausible biosynthetic pathways for compounds 1-3 are proposed.Surprisingly,compound 1 exhibited cytotoxicity against SGC-7901 cells by inducing cells apoptosis,which might relate to the promotion of synthesis of neutrophil elastase.展开更多
The study examined the inhibitory effect of Atractylodes macrocephala （AM） on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the C...The study examined the inhibitory effect of Atractylodes macrocephala （AM） on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the Ca^2＋-activated K^＋ currents of pregnant human myometrial smooth muscle cells with or without the treatment with intedeukin-6. Single cells were acutely isolated from pregnant human myometrial smooth muscles. Whole-cell Ca^2＋-activated K^＋ currents were recorded by using an Axopatchl-D amplifier. The cells were divided into three groups： group A in which AM was added into perfusate, group B, in which interleukin-6 was added into perfusate） and group C in which AM was added into perfusate after addition of interleukin-6. IL-6 10 ng/mL inhibited BKca by 36.9%±13.7% as compared with control （P〈0.01）. AM at 2 mg/mL raised BKca by 36.7%±22.6% or 45.2%±13.7% with or without the treatment of IL-6, respectively （P〈0.01）. It is concluded that AM was able to enhance the BKca of pregnant human myometrial smooth muscle cells treated or untreated with interleukin-6 and its effect on the BKca IL-treated cells was stronger that its effect on BKca of untreated cells. Our results suggested that AM can help to maintain the membrane potentials and the resting status of pregnant human myometrial smooth muscle cells.展开更多
Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contain...Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contains volatile oil,atractylenolides,atractylodes polysaccharides,glycosides and amino acids.And it has the medical functions of good for spleen and intestine,diuretic and dehumidification,hidroschesis,miscarriage prevention and soon.In order to provide references for further development and utilization,this paper systematic arranged the Chinese medicine atractylodes chemical composition,pharmacological effects,processing technology and effect of processing technology on chemical composition and pharmacological action.展开更多
[Objective] The paper was to explore the effective agent against seedling blight of Atractylodes macrocephala. [Method] The control effects of 60% validamycin A SP against seeding blight of A. macrocephala were studie...[Objective] The paper was to explore the effective agent against seedling blight of Atractylodes macrocephala. [Method] The control effects of 60% validamycin A SP against seeding blight of A. macrocephala were studied in the field, and compared with that of control agent 20% bismerthiazol WP. [Result] At 14 d post the third spraying, the control effect of 60% validamycin A SP at the dose of 495-540 g/hm;was 76.9%,which was equivalent to that of control agent 20% bismerthiazol WP sprayed at the dose of 270 g/hm;（76.7%）. [Conclusion] 60% Validamycin A SP could be used as the ideal agent against seedling blight of A. macrocephala, with good prospects of application and popularization.展开更多
[Objectives]The paper was to explore the pharmacodynamic substances and mechanism of Atractylodes macrocephala in treating primary dysmenorrhea(PD).[Methods]The components of A.macrocephala were qualitatively identifi...[Objectives]The paper was to explore the pharmacodynamic substances and mechanism of Atractylodes macrocephala in treating primary dysmenorrhea(PD).[Methods]The components of A.macrocephala were qualitatively identified by ultra high performance liquid chromatography quadrupole-time of flight mass spectrometry(UPLC-MS)combined with analyst TF 1.7.1 and peakview 2.2 software with reference to internal databases and literatures.The chemical components of A.macrocephala and the target of PD were collected by using network pharmacological data.The common genes were analyzed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)of genes and genomes with the help of String database and Metascape platform,and the affinity between core molecules and key targets was verified.[Results]A total of 23 chemical components of A.macrocephala were identified,and 301 gene targets of chemical components of A.macrocephala,614 targets of PD,and 25 intersection targets were obtained.GO analysis results obtained 505 biological processes,11 cellular components,and 33 molecular functions.KEGG pathway analysis showed that it mainly involved TNF signaling pathway,IL-17 signaling pathway,estrogen receptor signaling pathway and arachidonic acid metabolism.The top 4 targets of PPI network centrality and the top 5 compounds of A.macrocephala-component-target-disease network centrality were selected for docking.The docking results showed that atractylenolide I,Selinar-4(15),7(11)-dien-8-one,and atractylenolide II had strong binding ability.[Conclusions]A.macrocephala may exert a curative effect on PD by targeting atractylenolide I,Selinar-4(15),7(11)-dien-8-one,and atractylenolide II on key targets such as TNF,IL6,IL1β,PTGS2 to regulate cellular TNF signaling pathway,IL-17 signaling pathway,estrogen receptor signaling pathway,and arachidonic acid metabolism.展开更多
Objective: to study the effect of different conditions on the content change and internal law of Atractylenolide I, II and III. Methods: the samples of Atractylodes macrocephala Koidz were treated with different bakin...Objective: to study the effect of different conditions on the content change and internal law of Atractylenolide I, II and III. Methods: the samples of Atractylodes macrocephala Koidz were treated with different baking temperatures, baking time, humidity, open space time, sample morphology and other conditions. The contents of Atractylodes macrocephala Koidz I, II and III were determined by HPLC, and the change rules were analyzed. Results: for the original medicinal materials and decoction pieces, temperature and heating time had a certain effect on the conversion amount, but the effect was limited. However, under the influence of the appropriate heating temperature and heating time, the content of pulverized sample powder changed significantly. In the case of Atractylodes macrocephala Koidz in the form of powder, with the increase of storage time, the content of Atractylenolide I, II and III changed significantly. Conclusion: comparison of the content change law of Atractylodes macrocephala Koidz lactone I, II, III is conducive to optimizing the processing method of Atractylodes macrocephala Koidz, and provides a reference for the quality control of Atractylodes macrocephala Koidz.展开更多
We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced o...We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced on Murashige-Skoog(MS)medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea(TDZ),6-benzylaminopurine(BA)andα-naphthaleneacetic acid(NAA).Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid(IBA).The maximum mean number of shoots(5.61)was obtained from a single explant by the combined effect of 1.08μmol/L NAA and 2.25μmol/L TDZ.The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators.The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7μmol/L NAA.展开更多
Two new sesquiterpenes: beishulenolide A 1 and peroxiatractylenolide III 2 wereisolated from Atraclylodes macrocephala Koids (compositae).Their structures were elucidated onthe basis of spectral evidence, especially 2...Two new sesquiterpenes: beishulenolide A 1 and peroxiatractylenolide III 2 wereisolated from Atraclylodes macrocephala Koids (compositae).Their structures were elucidated onthe basis of spectral evidence, especially 2D-NMR methods and chemical conversion.展开更多
Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, trad...Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, traditional Chinese medicine has become a feasible alternative for certain diseases, including recurrent spontaneous abortion. However, because of the complex composition of the traditional Chinese medicine formula, its action mechanism remains unclear. Methods: We selected two isolated active ingredients (RAMP and baicalin) from the traditional Chinese medicine formula and used an abortion-prone CBA/J × DBA/2 model to simulate human RSA and compared the changes in fetal resorption rate, Treg cell percentage, and relevant cytokines before and after combination therapy. In addition, The mechanisms were preliminarily discussed using in vitro differentiation models. Results: In CBA/J × DBA/2 abortion-prone mice, the combination therapy resulted in a lower embryo resorption rate compared to that obtained with individual delivery of either RAMP or baicalin, thereby playing an embryo-protective role through the increase in Treg cells for the maintenance of maternal-fetal immune tolerance. In in vitro primary cell differentiation experiments, the concentration of Treg cells significantly increased from 11% to 17.9% after the combination therapy compared to that of the single administration group. Conclusion: the synergistic effects of RAMP and baicalin were responsible for Treg differentiation. The present study provides a solid basis for improving the applicability of traditional Chinese herbs in the treatment of recurrent spontaneous abortion.展开更多
Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for stren...Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for strengthening the spleen and harmonizing the stomach.BZ processed with Aurantii Fructus Immaturus juice(ZSZBZ),a classic decoction since the Han Dynasty,can enhance the efficacy of BZ.However,the key active components and targets of action of ZSZBZ remain undiscovered.Aimof the study:This study aimed to investigate the bioactive chemical constituents of ZSZBZ against AGU and their possible mechanisms of action,elucidating the scientific content of ZSZBZ processing.Materials and methods:Initially,we examined rat stomach histopathology and conducted ELISA for oxidative stress and inflammation.Subsequently,we investigated underlying mechanisms using metabolomics.Further analysis of potent components and key targets in ZSZBZ was conducted through liquid chromatography-mass spectrometry analysis combined with network pharmacology.Finally,key targets were analyzed by Western blot.Results:ZSZBZ improved gastric histopathology,reversing high alcohol-induced oxidative stress(SOD,CAT)and inflammatory level(TNF-α,IL-6)disorders.This is associated with ZSZBZ’s regulation of amino acid metabolism,energy metabolism,and inflammatory response-related metabolic pathways,along with key targets PTGS2,MAPK1,and KDR.The significant increase in potency of ZSZBZ may be attributed to elevated levels of naringenin,hesperidin,hesperidin,and rhamnoceroside after concoction.Conclusions:Combining metabolomics and network pharmacology,this study elucidated that ZSZBZ enhanced gastroprotection by modulating amino acid metabolism,antioxidant,and inflammation-related targets and pathways,providing insights into the bioactive compounds and potential mechanisms of herbal concoctions.展开更多
Objective:To observe the clinical efficacy of tuina at head and abdomen for headache due to phlegm turbidity.Methods:A total of 56 patients with headache due to phlegm turbidity were randomized into a tuina group and ...Objective:To observe the clinical efficacy of tuina at head and abdomen for headache due to phlegm turbidity.Methods:A total of 56 patients with headache due to phlegm turbidity were randomized into a tuina group and a Chinese medicine group by the random number table,with 28 cases in each group.The tuina group was treated mainly with tuina at the head and abdomen,while the Chinese medicine group was treated with oral administration of Ban Xia Bai Zhu Tian Ma Tang(Pinellio,Atractylodes Mocrocepholo and Gastrodio Decoction).The course of treatment was 30 d.The scores of headache index,traditional Chinese medicine syndrome scale,and the therapeutic efficacy were observed.Results:There were 2 dropouts in each group during treatment.The total effective rate was 92.3%in the tuina group,significantly higher than 76.9%in the Chinese medicine group(P<0.05).The scores of headache index and traditional Chinese medicine syndrome scale in both groups decreased after treatment(both P<0.05),and scores in the tuina group were lower than those in the Chinese medicine group(both P<0.05).Conclusion:Tuina mainly at head and abdomen is effective in treating headache due to phlegm turbidity,and has a better effect than Ban Xia Bai Zhu Tian Ma Tang(Pinellia,Atractylodes Macrocephalo and Gastrodio Decoction).展开更多
基金Natural Science Foundation of Zhejiang Province,Grant/Award Number:Y19H280022。
文摘Background:In a previous study,we found that Atractylodes macrocephala and Paeoniae radix(AM-PR)was useful for the alleviation of functional constipation(FC).However,the precise mechanism underlying the compatibility between AM and PR in the treatment of FC remains uncertain.This study aims to analyze the pharmacokinetic differences in the active ingredients in the blood of rat models with FC when applied individually and in combination with AM-PR.It also seeks to compare the changes in the content of the active ingredient when applied individually and in combination with in vitro AM-PR,further in-depth investigation into its material foundation in terms of pharmacokinetics,as well as the composition of the substance.Methods:Blood microdialysis samples were collected using microdialysis technology.These samples from rats with FC were compared after administration of AM,PR,and AM-PR.The concentration of the main active ingredients was determined using the Ultra Performance Liquid Chromatography-Tunable Ultraviolet(UPLC-TUV)method.The concentration of the main active ingredients of the decoction compatibility before and after combining AM-PR was also determined using the UPLC-TUV method.Results:Our findings reveal that upon combination,the time to maximum concentration(T_(max))of isochlorogenic acid A(ICA-A)and ataridolide Ⅱ(ATR-Ⅱ)T_(max)was prolonged,terminal elimination half-life(T_(1/2))was reduced,and maximum plasma concentrations(C_(max))increased.The T_(max)of ataridolide Ⅲ(ATR-Ⅲ)remained consistent,whereas its T_(1/2)and C_(max)were significantly reduced.Conversely,for peoniflorin(PAE),T_(max)occurred sooner,T_(1/2)was shortened,and C_(max)increased.The T_(max)for albiflorin(ALB)remained consistent,whereas T_(1/2)and C_(max)witnessed significant increases.The area under the moment curve(AUMC)(0-t)and AUMC(0-∞)of PAE,ALB,ICA-A,ATR-Ⅱ experienced an increase after AM-PR administration in rats,attributable to the heightened C_(max).In comparison to individual herb administration,the T_(max)of ALB was advanced in combination,the T_(max)of PAE remained unchanged,and the T_(max)of ICA-A and ART-Ⅱ was delayed,with an increased area under the concentration-time curve(AUC)(0-t),indicating enhanced C_(max)and bioavailability.Furthermore,the dissolution rates of PAE,ICA-A,and ATR-Ⅱ significantly improved after compatibility.Conclusions:This study partially clarifies the rationale and compatibility of AM-PR in treating FC and offers a new perspective on the pharmacokinetic interactions of AM-PR in FC treatment.
文摘First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.
基金supported by the National Natural Science Foundation of China(No.82073992)the CAMS Innovation Fund for Medical Sciences(CIFMS,No.2021-I2M-1-071)。
文摘Three eudesmanolide sesquiterpene-phenol hybrids,atramacronoids A-C(1-3),featuring an unusual6/6/5/5/6 skeleton furnished by forming an unexpected C-8-C-16 linkage,were obtained from the rhizomes of Atractylodes macrocephala.Their structures and absolute configurations were elucidated by spectroscopic data analysis,chemical calculations,combined with X-ray diffractions.The plausible biosynthetic pathways for compounds 1-3 are proposed.Surprisingly,compound 1 exhibited cytotoxicity against SGC-7901 cells by inducing cells apoptosis,which might relate to the promotion of synthesis of neutrophil elastase.
文摘The study examined the inhibitory effect of Atractylodes macrocephala （AM） on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the Ca^2＋-activated K^＋ currents of pregnant human myometrial smooth muscle cells with or without the treatment with intedeukin-6. Single cells were acutely isolated from pregnant human myometrial smooth muscles. Whole-cell Ca^2＋-activated K^＋ currents were recorded by using an Axopatchl-D amplifier. The cells were divided into three groups： group A in which AM was added into perfusate, group B, in which interleukin-6 was added into perfusate） and group C in which AM was added into perfusate after addition of interleukin-6. IL-6 10 ng/mL inhibited BKca by 36.9%±13.7% as compared with control （P〈0.01）. AM at 2 mg/mL raised BKca by 36.7%±22.6% or 45.2%±13.7% with or without the treatment of IL-6, respectively （P〈0.01）. It is concluded that AM was able to enhance the BKca of pregnant human myometrial smooth muscle cells treated or untreated with interleukin-6 and its effect on the BKca IL-treated cells was stronger that its effect on BKca of untreated cells. Our results suggested that AM can help to maintain the membrane potentials and the resting status of pregnant human myometrial smooth muscle cells.
基金supported by National Natural Science Foundation of China(81473549)National Natural Science Foundation of China Youth Fund Project(31402237)+1 种基金National "Major New Drug Creation" Major Science and Technology Projects(2014ZX09304-306-04)Ministry of Education of the Central University Basic Scientific Research Business Fee(XDJK2015D016)
文摘Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contains volatile oil,atractylenolides,atractylodes polysaccharides,glycosides and amino acids.And it has the medical functions of good for spleen and intestine,diuretic and dehumidification,hidroschesis,miscarriage prevention and soon.In order to provide references for further development and utilization,this paper systematic arranged the Chinese medicine atractylodes chemical composition,pharmacological effects,processing technology and effect of processing technology on chemical composition and pharmacological action.
文摘[Objective] The paper was to explore the effective agent against seedling blight of Atractylodes macrocephala. [Method] The control effects of 60% validamycin A SP against seeding blight of A. macrocephala were studied in the field, and compared with that of control agent 20% bismerthiazol WP. [Result] At 14 d post the third spraying, the control effect of 60% validamycin A SP at the dose of 495-540 g/hm;was 76.9%,which was equivalent to that of control agent 20% bismerthiazol WP sprayed at the dose of 270 g/hm;（76.7%）. [Conclusion] 60% Validamycin A SP could be used as the ideal agent against seedling blight of A. macrocephala, with good prospects of application and popularization.
基金Youth Science Foundation Project of National Natural Science Foundation of China(82104384)Science and Technology Research Project of Colleges and Universities in Hebei Province(QN2021008)+3 种基金Research Start-up Fund for High-level Talents of Chengde Medical University(202103)Key Discipline Construction Project of Colleges and Universities in Hebei Province(JJG[2013]4)"Technology Innovation Guidance Special-Science and Technology Work Consultation"Project of Hebei Provincial Department of Science and TechnologyYouth PI Science and Technology Innovation Team of TCM Pharmacodynamic Substance Foundation of Chengde Medical University.
文摘[Objectives]The paper was to explore the pharmacodynamic substances and mechanism of Atractylodes macrocephala in treating primary dysmenorrhea(PD).[Methods]The components of A.macrocephala were qualitatively identified by ultra high performance liquid chromatography quadrupole-time of flight mass spectrometry(UPLC-MS)combined with analyst TF 1.7.1 and peakview 2.2 software with reference to internal databases and literatures.The chemical components of A.macrocephala and the target of PD were collected by using network pharmacological data.The common genes were analyzed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)of genes and genomes with the help of String database and Metascape platform,and the affinity between core molecules and key targets was verified.[Results]A total of 23 chemical components of A.macrocephala were identified,and 301 gene targets of chemical components of A.macrocephala,614 targets of PD,and 25 intersection targets were obtained.GO analysis results obtained 505 biological processes,11 cellular components,and 33 molecular functions.KEGG pathway analysis showed that it mainly involved TNF signaling pathway,IL-17 signaling pathway,estrogen receptor signaling pathway and arachidonic acid metabolism.The top 4 targets of PPI network centrality and the top 5 compounds of A.macrocephala-component-target-disease network centrality were selected for docking.The docking results showed that atractylenolide I,Selinar-4(15),7(11)-dien-8-one,and atractylenolide II had strong binding ability.[Conclusions]A.macrocephala may exert a curative effect on PD by targeting atractylenolide I,Selinar-4(15),7(11)-dien-8-one,and atractylenolide II on key targets such as TNF,IL6,IL1β,PTGS2 to regulate cellular TNF signaling pathway,IL-17 signaling pathway,estrogen receptor signaling pathway,and arachidonic acid metabolism.
文摘Objective: to study the effect of different conditions on the content change and internal law of Atractylenolide I, II and III. Methods: the samples of Atractylodes macrocephala Koidz were treated with different baking temperatures, baking time, humidity, open space time, sample morphology and other conditions. The contents of Atractylodes macrocephala Koidz I, II and III were determined by HPLC, and the change rules were analyzed. Results: for the original medicinal materials and decoction pieces, temperature and heating time had a certain effect on the conversion amount, but the effect was limited. However, under the influence of the appropriate heating temperature and heating time, the content of pulverized sample powder changed significantly. In the case of Atractylodes macrocephala Koidz in the form of powder, with the increase of storage time, the content of Atractylenolide I, II and III changed significantly. Conclusion: comparison of the content change law of Atractylodes macrocephala Koidz lactone I, II, III is conducive to optimizing the processing method of Atractylodes macrocephala Koidz, and provides a reference for the quality control of Atractylodes macrocephala Koidz.
基金This work was partially funded by the Science and Technology Department of Zhejiang Province,China(Nos.2005C13016 and 2005C22080).
文摘We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced on Murashige-Skoog(MS)medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea(TDZ),6-benzylaminopurine(BA)andα-naphthaleneacetic acid(NAA).Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid(IBA).The maximum mean number of shoots(5.61)was obtained from a single explant by the combined effect of 1.08μmol/L NAA and 2.25μmol/L TDZ.The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators.The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7μmol/L NAA.
文摘Two new sesquiterpenes: beishulenolide A 1 and peroxiatractylenolide III 2 wereisolated from Atraclylodes macrocephala Koids (compositae).Their structures were elucidated onthe basis of spectral evidence, especially 2D-NMR methods and chemical conversion.
基金the National Natural Science Foundation of China(81973221)National Natural Science Foundation for Young Scientists of China(81603647)+2 种基金the Women and Children Health Talent Project of Jiangsu Province(FRC201785)the Chinese Clinical Medicine Innovation Center of Obstetrics,Gynecology,and Reproduction in Jiangsu Province(ZX202102)the Women and Children Health Science Foundation of Jiangsu Province(F202206).
文摘Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, traditional Chinese medicine has become a feasible alternative for certain diseases, including recurrent spontaneous abortion. However, because of the complex composition of the traditional Chinese medicine formula, its action mechanism remains unclear. Methods: We selected two isolated active ingredients (RAMP and baicalin) from the traditional Chinese medicine formula and used an abortion-prone CBA/J × DBA/2 model to simulate human RSA and compared the changes in fetal resorption rate, Treg cell percentage, and relevant cytokines before and after combination therapy. In addition, The mechanisms were preliminarily discussed using in vitro differentiation models. Results: In CBA/J × DBA/2 abortion-prone mice, the combination therapy resulted in a lower embryo resorption rate compared to that obtained with individual delivery of either RAMP or baicalin, thereby playing an embryo-protective role through the increase in Treg cells for the maintenance of maternal-fetal immune tolerance. In in vitro primary cell differentiation experiments, the concentration of Treg cells significantly increased from 11% to 17.9% after the combination therapy compared to that of the single administration group. Conclusion: the synergistic effects of RAMP and baicalin were responsible for Treg differentiation. The present study provides a solid basis for improving the applicability of traditional Chinese herbs in the treatment of recurrent spontaneous abortion.
基金funded by the Jiangxi Provincial Natural Science Foundation Youth Project(20212BAB216011)the Second National Famous ChineseMedicine Practitioner(Gong Qianfeng)Inheritance Workshop of the State Administration of Traditional ChineseMedicine(State Chinese Medicine Office Human Education Letter[2022]no.245)+2 种基金2023 National Chinese Medicine Characteristic Technology Inheritance Talent Training Program(State Chinese Medicine Human Education Letter[2023]no.96)Jiangxi Province,the young and medium-aged backbone talents of traditional Chinese medicine(the fourth batch)(Gan TCM Science and Education[2022]no.7)the seventh batch of the national old Chinese medicine experts in academic experience successors(State Chinesemedicine human education letter[2022]no.76).
文摘Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for strengthening the spleen and harmonizing the stomach.BZ processed with Aurantii Fructus Immaturus juice(ZSZBZ),a classic decoction since the Han Dynasty,can enhance the efficacy of BZ.However,the key active components and targets of action of ZSZBZ remain undiscovered.Aimof the study:This study aimed to investigate the bioactive chemical constituents of ZSZBZ against AGU and their possible mechanisms of action,elucidating the scientific content of ZSZBZ processing.Materials and methods:Initially,we examined rat stomach histopathology and conducted ELISA for oxidative stress and inflammation.Subsequently,we investigated underlying mechanisms using metabolomics.Further analysis of potent components and key targets in ZSZBZ was conducted through liquid chromatography-mass spectrometry analysis combined with network pharmacology.Finally,key targets were analyzed by Western blot.Results:ZSZBZ improved gastric histopathology,reversing high alcohol-induced oxidative stress(SOD,CAT)and inflammatory level(TNF-α,IL-6)disorders.This is associated with ZSZBZ’s regulation of amino acid metabolism,energy metabolism,and inflammatory response-related metabolic pathways,along with key targets PTGS2,MAPK1,and KDR.The significant increase in potency of ZSZBZ may be attributed to elevated levels of naringenin,hesperidin,hesperidin,and rhamnoceroside after concoction.Conclusions:Combining metabolomics and network pharmacology,this study elucidated that ZSZBZ enhanced gastroprotection by modulating amino acid metabolism,antioxidant,and inflammation-related targets and pathways,providing insights into the bioactive compounds and potential mechanisms of herbal concoctions.
文摘Objective:To observe the clinical efficacy of tuina at head and abdomen for headache due to phlegm turbidity.Methods:A total of 56 patients with headache due to phlegm turbidity were randomized into a tuina group and a Chinese medicine group by the random number table,with 28 cases in each group.The tuina group was treated mainly with tuina at the head and abdomen,while the Chinese medicine group was treated with oral administration of Ban Xia Bai Zhu Tian Ma Tang(Pinellio,Atractylodes Mocrocepholo and Gastrodio Decoction).The course of treatment was 30 d.The scores of headache index,traditional Chinese medicine syndrome scale,and the therapeutic efficacy were observed.Results:There were 2 dropouts in each group during treatment.The total effective rate was 92.3%in the tuina group,significantly higher than 76.9%in the Chinese medicine group(P<0.05).The scores of headache index and traditional Chinese medicine syndrome scale in both groups decreased after treatment(both P<0.05),and scores in the tuina group were lower than those in the Chinese medicine group(both P<0.05).Conclusion:Tuina mainly at head and abdomen is effective in treating headache due to phlegm turbidity,and has a better effect than Ban Xia Bai Zhu Tian Ma Tang(Pinellia,Atractylodes Macrocephalo and Gastrodio Decoction).
