Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood supply.Previous research has suggested that au-tophagy and angiogenesis play vital roles in the preven...Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood supply.Previous research has suggested that au-tophagy and angiogenesis play vital roles in the prevention of heart failure after MI,and miR-106a is considered to be an important regulatory factor in MI.But the specific mechanism remains unknown.In this study,using cultured venous endothelial cells and a rat model of MI,we aimed to identify the potential target genes of miR-106a and discover the mechanisms of inhibiting autophagy and angiogenesis.Methods:We first explored the biological functions of miR-106a on autophagy and angiogenesis on endothelial cells.Then we identified ATG7,which was the down-stream target gene of miR-106a.The expression of miR-106a and ATG7 was investi-gated in the rat model of MI.Results:We found that miR-106a inhibits the proliferation,cell cycle,autophagy and angiogenesis,but promoted the apoptosis of vein endothelial cells.Moreover,ATG7 was identified as the target of miR-106a,and ATG7 rescued the inhibition of autophagy and angiogenesis by miR-106a.The expression of miR-106a in the rat model of MI was decreased but the expression of ATG7 was increased in the infarction areas.Conclusion:Our results indicate that miR-106a may inhibit autophagy and angiogenesis by targeting ATG7.This mechanism may be a potential therapeutic treatment for MI.展开更多
目的:探讨糖尿病肾病(DKD)患者血清Beclin1、ATG7水平与蛋白尿的相关性。方法:收集2018年12月—2019年10月在本院门诊或住院部诊断为DKD共108例患者的临床资料,按ACR分为正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组。纳入同期我院...目的:探讨糖尿病肾病(DKD)患者血清Beclin1、ATG7水平与蛋白尿的相关性。方法:收集2018年12月—2019年10月在本院门诊或住院部诊断为DKD共108例患者的临床资料,按ACR分为正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组。纳入同期我院体检健康者共30例为对照组。检测血清Beclin1、ATG7水平,用Pearson/Spearman相关分析Beclin1、ATG7与尿微量白蛋白、尿微量白蛋白/肌酐等指标的相关性。用多重线性逐步回归法分析Beclin1、ATG7的影响因素。结果:(1)与对照组相比,DKD三组患者血清Beclin1、ATG7水平均降低,其中大量白蛋白尿组最低(P<0.001)。(2)Beclin1与HDL-C、eGFR、Hb、Alb呈正相关(P<0.05),与糖尿病病程、HbAlc、PBG、TC、BUN、Scr、UA、UAlb、ACR、24 h UTP呈负相关(P<0.05)。ATG7与HDL-C、eGFR、Hb、Alb呈正相关(P<0.05),与糖尿病病程、FBG、HbAlc、PBG、TC、LDL-C、BUN、Scr、UA、UAlb、ACR、24 h UTP呈负相关(P<0.05)。(3)经多重线性逐步回归分析,Beclin1水平的重要预测指标为糖尿病病程、ACR、eGFR、HDL-C(P<0.01)。ATG7水平的预测指标为ACR、eGFR、HbAlc(P<0.05)。结论:本研究发现DKD患者血清中自噬相关蛋白Beclin1、ATG7水平降低,与蛋白尿程度存在相关性,对于明确自噬障碍与DKD进展可能具有一定的研究价值,其具体机制有待于进一步研究。展开更多
基金National Natural Science Foundation of China,Grant/Award Number:32070542Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2021A1515010873 and 2022A1515011455+1 种基金Breed Industry Innovation Park of Guangdong Xiaoerhua Pig,Grant/Award Number:2022-4408X1-43010402-0019Hainan Provincial Natural Science Foundation,Grant/Award Number:818MS132。
文摘Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood supply.Previous research has suggested that au-tophagy and angiogenesis play vital roles in the prevention of heart failure after MI,and miR-106a is considered to be an important regulatory factor in MI.But the specific mechanism remains unknown.In this study,using cultured venous endothelial cells and a rat model of MI,we aimed to identify the potential target genes of miR-106a and discover the mechanisms of inhibiting autophagy and angiogenesis.Methods:We first explored the biological functions of miR-106a on autophagy and angiogenesis on endothelial cells.Then we identified ATG7,which was the down-stream target gene of miR-106a.The expression of miR-106a and ATG7 was investi-gated in the rat model of MI.Results:We found that miR-106a inhibits the proliferation,cell cycle,autophagy and angiogenesis,but promoted the apoptosis of vein endothelial cells.Moreover,ATG7 was identified as the target of miR-106a,and ATG7 rescued the inhibition of autophagy and angiogenesis by miR-106a.The expression of miR-106a in the rat model of MI was decreased but the expression of ATG7 was increased in the infarction areas.Conclusion:Our results indicate that miR-106a may inhibit autophagy and angiogenesis by targeting ATG7.This mechanism may be a potential therapeutic treatment for MI.
文摘目的:探讨糖尿病肾病(DKD)患者血清Beclin1、ATG7水平与蛋白尿的相关性。方法:收集2018年12月—2019年10月在本院门诊或住院部诊断为DKD共108例患者的临床资料,按ACR分为正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组。纳入同期我院体检健康者共30例为对照组。检测血清Beclin1、ATG7水平,用Pearson/Spearman相关分析Beclin1、ATG7与尿微量白蛋白、尿微量白蛋白/肌酐等指标的相关性。用多重线性逐步回归法分析Beclin1、ATG7的影响因素。结果:(1)与对照组相比,DKD三组患者血清Beclin1、ATG7水平均降低,其中大量白蛋白尿组最低(P<0.001)。(2)Beclin1与HDL-C、eGFR、Hb、Alb呈正相关(P<0.05),与糖尿病病程、HbAlc、PBG、TC、BUN、Scr、UA、UAlb、ACR、24 h UTP呈负相关(P<0.05)。ATG7与HDL-C、eGFR、Hb、Alb呈正相关(P<0.05),与糖尿病病程、FBG、HbAlc、PBG、TC、LDL-C、BUN、Scr、UA、UAlb、ACR、24 h UTP呈负相关(P<0.05)。(3)经多重线性逐步回归分析,Beclin1水平的重要预测指标为糖尿病病程、ACR、eGFR、HDL-C(P<0.01)。ATG7水平的预测指标为ACR、eGFR、HbAlc(P<0.05)。结论:本研究发现DKD患者血清中自噬相关蛋白Beclin1、ATG7水平降低,与蛋白尿程度存在相关性,对于明确自噬障碍与DKD进展可能具有一定的研究价值,其具体机制有待于进一步研究。
