目的探讨洛桑卒中量表(Acute Stroke Registry and Analysis of Lausanne,ASTRAL)评分预测不同性别、不同梗死部位、不同病情严重程度急性缺血性卒中(acute ischemic stroke,AIS)患者3个月预后的价值,并与PLAN(Preadmission Comorbiditi...目的探讨洛桑卒中量表(Acute Stroke Registry and Analysis of Lausanne,ASTRAL)评分预测不同性别、不同梗死部位、不同病情严重程度急性缺血性卒中(acute ischemic stroke,AIS)患者3个月预后的价值,并与PLAN(Preadmission Comorbidities,Level of Consciousness,Age,Neurologic Deficit,PLAN)评分、血管事件总体健康风险(Totaled Health Risks in Vascular Events,THRIVE)评分对急性缺血性卒中3个月预测价值进行比较。方法记录符合纳入标准的急性缺血性卒中患者的基线资料,按照性别、梗死部位、病情严重程度进行分组,然后记录并分别使用ASTRAL评分、PLAN评分与THRIVE评分预测患者3个月预后。通过受试者工作特征曲线下面积(area under the receiver operating characteristic curve,AUC)进行Z检验来比较它们的预测价值。结果 1本研究共纳入513例AIS患者,男性309例、女性204例;梗死部位:前循环305例,后循环153例,混合型55例;病情轻重程度:轻型卒中260例,非轻型卒中253例;2ASTRAL预测所有入组AIS患者的AUC为0.845,预测男性组AUC为0.804,女性组AUC为0.878,两者比较Z值为1.86(P>0.05);预测前循环组AUC为0.843、后循环组AUC分别为0.857、混合组AUC为0.814,三者比较Z值均<1.98(P>0.05);预测轻型卒中AUC为0.714、非轻型卒中AUC为0.855,两者比较Z值为4.03,其值>1.98(P值为0.000)。ASTRAL评分、PLAN评分、THRIVE评分预测所有入组AIS患者的AUC分别为0.845、0.783和0.740,三者比较Z值均>1.98(P值分别为0.000、0.010和0.000)。结论 ASTRAL评分可对AIS患者3个月预后进行预测,更适合非轻型AIS患者,预测价值高于PLAN评分、THRIVE评分。展开更多
Background Toxocara canis,the most prevalent helminth in dogs and other canines,is one of the socioeconomically important zoonotic parasites,particularly affecting pediatric and adolescent populations in impoverished ...Background Toxocara canis,the most prevalent helminth in dogs and other canines,is one of the socioeconomically important zoonotic parasites,particularly affecting pediatric and adolescent populations in impoverished communities.However,limited information is available regarding the proteomes of female and male adult T.canis.To address this knowledge gap,we performed a comprehensive proteomic analysis to identify the proteins with differential abundance(PDAs)and gender-specifically expressed proteins between the two sexes adult T.canis.Methods The comparative proteomic analysis was carried out by the Orbitrap mass spectrometry(MS)with asymmetric track lossless(Astral)analyzer.The difference analysis was conducted using t-test and the proteins verification was achieved through parallel reaction monitoring(PRM).The potential biological functions of identified adult T.canis proteins and PDAs were predicted by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases.The domain,transcription factor and subcellular localization of the identified proteins and PDAs were analyzed by InterPro,AnimalTFDB 4.0 and Cell-mPLOC 2.0 databases,respectively.Results A total of 8565 somatic proteins of adult T.canis were identified.Compared to male adult,682 up-regulated PDAs and 844 down-regulated PDAs were identified in female adult with P-values<0.05 and|log2FC|>1,including 139 proteins exclusively expressed in female and 272 proteins exclusively expressed in male.The GO annotation analysis using all PDAs revealed that the main biological processes,cellular components and molecular functions corresponded to aminoglycan metabolic process,extracellular region and protein tyrosine phosphatase activity,respectively.The KEGG analysis using all PDAs showed that the pathways were mainly associated with adipocytokine signaling pathway,proximal tubule bicarbonate reclamation and PPAR signaling pathway.Conclusions This study reveals the differential protein expression between female and male adult T.canis,providing valuable resource for developing the novel intervention strategies against T.canis infection in humans and animals,especially from the perspective of sexual development and reproduction.展开更多
文摘目的探讨洛桑卒中量表(Acute Stroke Registry and Analysis of Lausanne,ASTRAL)评分预测不同性别、不同梗死部位、不同病情严重程度急性缺血性卒中(acute ischemic stroke,AIS)患者3个月预后的价值,并与PLAN(Preadmission Comorbidities,Level of Consciousness,Age,Neurologic Deficit,PLAN)评分、血管事件总体健康风险(Totaled Health Risks in Vascular Events,THRIVE)评分对急性缺血性卒中3个月预测价值进行比较。方法记录符合纳入标准的急性缺血性卒中患者的基线资料,按照性别、梗死部位、病情严重程度进行分组,然后记录并分别使用ASTRAL评分、PLAN评分与THRIVE评分预测患者3个月预后。通过受试者工作特征曲线下面积(area under the receiver operating characteristic curve,AUC)进行Z检验来比较它们的预测价值。结果 1本研究共纳入513例AIS患者,男性309例、女性204例;梗死部位:前循环305例,后循环153例,混合型55例;病情轻重程度:轻型卒中260例,非轻型卒中253例;2ASTRAL预测所有入组AIS患者的AUC为0.845,预测男性组AUC为0.804,女性组AUC为0.878,两者比较Z值为1.86(P>0.05);预测前循环组AUC为0.843、后循环组AUC分别为0.857、混合组AUC为0.814,三者比较Z值均<1.98(P>0.05);预测轻型卒中AUC为0.714、非轻型卒中AUC为0.855,两者比较Z值为4.03,其值>1.98(P值为0.000)。ASTRAL评分、PLAN评分、THRIVE评分预测所有入组AIS患者的AUC分别为0.845、0.783和0.740,三者比较Z值均>1.98(P值分别为0.000、0.010和0.000)。结论 ASTRAL评分可对AIS患者3个月预后进行预测,更适合非轻型AIS患者,预测价值高于PLAN评分、THRIVE评分。
文摘Background Toxocara canis,the most prevalent helminth in dogs and other canines,is one of the socioeconomically important zoonotic parasites,particularly affecting pediatric and adolescent populations in impoverished communities.However,limited information is available regarding the proteomes of female and male adult T.canis.To address this knowledge gap,we performed a comprehensive proteomic analysis to identify the proteins with differential abundance(PDAs)and gender-specifically expressed proteins between the two sexes adult T.canis.Methods The comparative proteomic analysis was carried out by the Orbitrap mass spectrometry(MS)with asymmetric track lossless(Astral)analyzer.The difference analysis was conducted using t-test and the proteins verification was achieved through parallel reaction monitoring(PRM).The potential biological functions of identified adult T.canis proteins and PDAs were predicted by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases.The domain,transcription factor and subcellular localization of the identified proteins and PDAs were analyzed by InterPro,AnimalTFDB 4.0 and Cell-mPLOC 2.0 databases,respectively.Results A total of 8565 somatic proteins of adult T.canis were identified.Compared to male adult,682 up-regulated PDAs and 844 down-regulated PDAs were identified in female adult with P-values<0.05 and|log2FC|>1,including 139 proteins exclusively expressed in female and 272 proteins exclusively expressed in male.The GO annotation analysis using all PDAs revealed that the main biological processes,cellular components and molecular functions corresponded to aminoglycan metabolic process,extracellular region and protein tyrosine phosphatase activity,respectively.The KEGG analysis using all PDAs showed that the pathways were mainly associated with adipocytokine signaling pathway,proximal tubule bicarbonate reclamation and PPAR signaling pathway.Conclusions This study reveals the differential protein expression between female and male adult T.canis,providing valuable resource for developing the novel intervention strategies against T.canis infection in humans and animals,especially from the perspective of sexual development and reproduction.
