Objective:To explore and analyze the effective component,target and mechanism of action of Radix Astragali-Radix Pseudostellariae in the treatment of chronic heart failure(CHF)by network pharmacological research metho...Objective:To explore and analyze the effective component,target and mechanism of action of Radix Astragali-Radix Pseudostellariae in the treatment of chronic heart failure(CHF)by network pharmacological research methods.Methods:With the help of TCMSP database,the effective chemical constituents and targets of Radix Astragali-Radix Pseudostellariae were screened out,and CHF-related proteins were obtained by Gene Cards and OMIM database.The target protein interaction network(PPI)was constructed on the String database platform,and using DAVID database to conduct the GO function and KEGG pathway enrichment analysis on the target of the obtained astragalus-pseudostellariae for the treatment of CHF.Results:Radix Astragali-Radix Pseudostellariae were selected as 28 effective chemical components and 104 therapeutic targets for CHF.There were obtained 49 GO functional annotations and 86 KEGG signaling pathways.Conclusion:Radix Astragali-Radix Pseudostellariae can treat CHF through multi-target and multi-pathway.展开更多
目的探索中药治疗脓毒症的“药-效”关联,识别与降低病死率、改善急性生理与慢性健康评分Ⅱ(acute physiology and chronic health evaluation Ⅱ,APACHEII)等关键临床结局相关的核心中药,为中药辅助治疗脓毒症提供循证参考。方法系统...目的探索中药治疗脓毒症的“药-效”关联,识别与降低病死率、改善急性生理与慢性健康评分Ⅱ(acute physiology and chronic health evaluation Ⅱ,APACHEII)等关键临床结局相关的核心中药,为中药辅助治疗脓毒症提供循证参考。方法系统检索截至2024年9月的9个中英文数据库,纳入中药复方治疗脓毒症的临床研究,构建脓毒症中药复方数据库。采用十折交叉验证比较9种机器学习模型的预测性能,并运用沙普利加性解释(Shapley additive explanations,SHAP)方法解析各临床结局的最优模型,识别关键药物及其贡献方向。结果多层感知器在预测中医证候评分、有效率、炎症与免疫指标、生化指标、器官功能障碍评分及病死率性能最佳;逻辑回归在血气分析和胃肠功能与肠黏膜屏上表现最优;支持向量机在血常规与APACHE Ⅱ评分上预测效能最佳。SHAP分析显示,地黄、枳实、黄芪、附子、黄芩、厚朴等中药与病死率、APACHE Ⅱ评分、炎症指标和胃肠功能等结局中具有较高正向贡献,位于“药-效”网络的核心节点。结论通过多模型比较与可解释性分析,构建了以临床结局为导向的中药“药-效”关联网络,揭示了核心中药在脓毒症治疗中的潜在关键作用,为中医药精准辨证用药及中药新药研发提供数据支撑和循证依据。展开更多
基金Fund Project:Youth Science Fund Project of National Natural Science Foundation of China(No.81904107)Scientific Research Fund of Heilongjiang University of Traditional Chinese Medicine(No.2019TD01)。
文摘Objective:To explore and analyze the effective component,target and mechanism of action of Radix Astragali-Radix Pseudostellariae in the treatment of chronic heart failure(CHF)by network pharmacological research methods.Methods:With the help of TCMSP database,the effective chemical constituents and targets of Radix Astragali-Radix Pseudostellariae were screened out,and CHF-related proteins were obtained by Gene Cards and OMIM database.The target protein interaction network(PPI)was constructed on the String database platform,and using DAVID database to conduct the GO function and KEGG pathway enrichment analysis on the target of the obtained astragalus-pseudostellariae for the treatment of CHF.Results:Radix Astragali-Radix Pseudostellariae were selected as 28 effective chemical components and 104 therapeutic targets for CHF.There were obtained 49 GO functional annotations and 86 KEGG signaling pathways.Conclusion:Radix Astragali-Radix Pseudostellariae can treat CHF through multi-target and multi-pathway.
