Objective:To identify and to determine the antimicrobial susceptibility of Acinetobacter baumannii(A.baumannii) clinical isolates from ICU at Aseer Central Hospital.Methods:The study was conducted in the Intensive Car...Objective:To identify and to determine the antimicrobial susceptibility of Acinetobacter baumannii(A.baumannii) clinical isolates from ICU at Aseer Central Hospital.Methods:The study was conducted in the Intensive Care Unit,Aseer Central Hospital,Saudi Arabia over 13 months period(2014-2015).Acinetobacter species(n= 105) were isolated from various clinical samples.Isolates were identified using selected phenotypic criteria and confirmed using the Vitek 2 automated system.This system was used to determine the susceptibilities of 21 antimicrobial agents.Patients,isolates and drug data were analyzed using the SPSS statistical software package to determine some epidemiological and microbiological patterns.Results:Of the 105 stains,A.baumannii accounted for 49(46.67%),A.baumannii complex,19(18.09%),A.baumannii/haemolyticus 32(30.47),Acinetobacter haemolyticus 4(3.81%),Acinetobater lwoffii 1(0.95%) and unidentified Acinetobater species 2(1.3%).Of the 105 Acinetobacter strains,103(98.1%) were found multidrug resistant(MDR).A.baumannii strain were 100% sensitive to colistin and 74.5% to trimethoprim + sulfamethoxazole.The remaining 19 antimicrobial agents revealed low or no sensitivities:amikacin 16.3%; ampicillin 7.7%; ceftazidime 7.3%.Distribution of similar sensitivities was shown by other Acinetobacter species.Mean number of isolates from males and females indicates no statistical variation(P=0.867) whereas age groups showed significant differences(P= 0.008) as it is clear from the high percentage of infected individuals more than 60 years followed by those aged 20 to 29 years old(19.05%).Upper respiratory tract(30.48%),lower respiratory tract(47.65%) and subcutaneous tissue(9.5%) were the main sources of Acinetobacter spp.but mean numbers of isolates from these specimens indicate no discrepancy between specimens(P=0.731).Conclusions:Acinetobacterspecies including A.baumannii were found MDR(98.1%) according to the current Acinetobacter spp.antimicrobial categorization.Approximately half of these strains were A.baumannii.All Acinetobacter species were 100% sensitive to colistin and to some extent to trimethoprim + sulfamethoxazole(74.5%).ICU-acquired pneumonia among patients over 60 years of age who spend prolong times at artificial ventilations made up the majority of the cases.展开更多
Objective:To investigate the prevalence and risk factors of brucellosis in human and animal’s communities in southern Saudi Arabia.Methods:A cross-sectional sero-epidemiological study was conducted in Aseer and Jazan...Objective:To investigate the prevalence and risk factors of brucellosis in human and animal’s communities in southern Saudi Arabia.Methods:A cross-sectional sero-epidemiological study was conducted in Aseer and Jazan,Saudi Arabia(October 2017-October 2018).Human serum samples(n=339)were initially screened for Brucella antibodies and positive samples were further titrated for Brucella antibodies by immunocapture assay(titer of≥1:320 as positive).Animal samples(n=828)were screened using the Rose Bengal test.Relationship status was dichotomized to measure and predict independent contributions to variations in human using univariate and multivariate stepwise binary logistic regression model.Results:The rate of brucellosis among the 339 human samples in the two regions was 33.9%,and the rate of acute brucellosis was 12.4%.The rate of brucellosis in animals was 4.7%.Human brucellosis among the target groups was higher in northwestern Aseer(53.3%)compared to Southeastern Aseer(25.9%)and Jazan region(20.6%).The disease was more prevalent among non-Saudi nationals(35.2%)compared to Saudis(30.5%).The rate of brucellosis among butchers and shepherds was 37.5%and 37.2%,respectively.The rate of brucellosis was 37.8%in people over 30 years of age.Our univariate analysis showed that residing in Aseer region(OR:2.60,95%CI:1.50-4.40),especially residing in northwestern Aseer region(OR:4.40,95%CI:2.40-7.90),frequent consumption of raw meat(OR:2.90,95%CI:1.50-5.50),shepherds(OR:2.10,95%CI:0.80-5.30),owning sheep(OR:2.20,95%CI:1.10-4.40),daily contact with animals(OR:2.10,95%CI:0.75-5.80),and those>30-year-old(OR:1.50,95%CI:1.00-2.40)were significantly associated with increased risks of brucellosis.Our multivariate analysis further showed that residing in northwestern Aseer(OR:9.16,95%CI:3.39-24.76)and having sheep(OR:1.16,95%CI:1.00-1.35)were significant and independent risks of brucellosis while residing in agricultural region(OR:0.28,95%CI:0.10-0.78)was a significant and independent protector against brucellosis.Conclusions:The study concluded that residing in northwestern Aseer area and having animals(sheep)are associated with significantly increased risks of brucellosis.展开更多
Background: Periodontitis is the predominant disease in the oral cavity and there are alterations in the periodontal tissues associated with the aging and gender of the patient. Objective: The present study was design...Background: Periodontitis is the predominant disease in the oral cavity and there are alterations in the periodontal tissues associated with the aging and gender of the patient. Objective: The present study was designed to assess the impact of age and gender of the patient on severity and types of periodontal diseases among patients from two regions in Saudi Arabia. Materials and Methods: The current study was done on 600 Saudi patients from Aseer and Tabuk regions in Saudi Arabia (50% males and 50% females) and divided into three equal groups (n = 200) according to the patients age: children and young patients (1 - 24 years old), adults patients (25 - 64 years old) and seniors patients (more than 65 years old). Plaque index (PLI), gingival index (GI), periodontal pocket depth (PPD) and clinical attachment loss (CAL) were recorded from all participants. The data were collected and analyzed with SPSS to determine the mean and standard deviation (±SD) and the values of significance (P ≤ 0.005). Results: The results of the present study revealed that 340 patients (56.7%) were affected with gingivitis dental biofilm induced and 260 patients were affected by periodontitis. Gingivitis dental biofilm-induced is especially detected in children and young patients (33.3%), adult patients (16.7%) and seniors patients (6.7%) whereas periodontitis cases were seen among the adult and seniors patients (16.7%) and (26.6%) respectively. Furthermore, the present study displayed the higher severity and prevalence of gingivitis dental biofilm-induced and periodontitis among females more than males in group II and group III maybe due to hormonal changes. The present study saw that there are statistically significant differences in clinical findings in the comparison between groups of this study (P ≤ 0.005). Conclusion: We conclude that there is a relation between severity and types of periodontal diseases and the ages and genders of Saudi patients in Aseer and Tabuk regions, Saudi Arabia.展开更多
文摘Objective:To identify and to determine the antimicrobial susceptibility of Acinetobacter baumannii(A.baumannii) clinical isolates from ICU at Aseer Central Hospital.Methods:The study was conducted in the Intensive Care Unit,Aseer Central Hospital,Saudi Arabia over 13 months period(2014-2015).Acinetobacter species(n= 105) were isolated from various clinical samples.Isolates were identified using selected phenotypic criteria and confirmed using the Vitek 2 automated system.This system was used to determine the susceptibilities of 21 antimicrobial agents.Patients,isolates and drug data were analyzed using the SPSS statistical software package to determine some epidemiological and microbiological patterns.Results:Of the 105 stains,A.baumannii accounted for 49(46.67%),A.baumannii complex,19(18.09%),A.baumannii/haemolyticus 32(30.47),Acinetobacter haemolyticus 4(3.81%),Acinetobater lwoffii 1(0.95%) and unidentified Acinetobater species 2(1.3%).Of the 105 Acinetobacter strains,103(98.1%) were found multidrug resistant(MDR).A.baumannii strain were 100% sensitive to colistin and 74.5% to trimethoprim + sulfamethoxazole.The remaining 19 antimicrobial agents revealed low or no sensitivities:amikacin 16.3%; ampicillin 7.7%; ceftazidime 7.3%.Distribution of similar sensitivities was shown by other Acinetobacter species.Mean number of isolates from males and females indicates no statistical variation(P=0.867) whereas age groups showed significant differences(P= 0.008) as it is clear from the high percentage of infected individuals more than 60 years followed by those aged 20 to 29 years old(19.05%).Upper respiratory tract(30.48%),lower respiratory tract(47.65%) and subcutaneous tissue(9.5%) were the main sources of Acinetobacter spp.but mean numbers of isolates from these specimens indicate no discrepancy between specimens(P=0.731).Conclusions:Acinetobacterspecies including A.baumannii were found MDR(98.1%) according to the current Acinetobacter spp.antimicrobial categorization.Approximately half of these strains were A.baumannii.All Acinetobacter species were 100% sensitive to colistin and to some extent to trimethoprim + sulfamethoxazole(74.5%).ICU-acquired pneumonia among patients over 60 years of age who spend prolong times at artificial ventilations made up the majority of the cases.
基金funded by a grant from the Deanship for Scientific Research,King Khalid University(Project#GRP-4-1439).
文摘Objective:To investigate the prevalence and risk factors of brucellosis in human and animal’s communities in southern Saudi Arabia.Methods:A cross-sectional sero-epidemiological study was conducted in Aseer and Jazan,Saudi Arabia(October 2017-October 2018).Human serum samples(n=339)were initially screened for Brucella antibodies and positive samples were further titrated for Brucella antibodies by immunocapture assay(titer of≥1:320 as positive).Animal samples(n=828)were screened using the Rose Bengal test.Relationship status was dichotomized to measure and predict independent contributions to variations in human using univariate and multivariate stepwise binary logistic regression model.Results:The rate of brucellosis among the 339 human samples in the two regions was 33.9%,and the rate of acute brucellosis was 12.4%.The rate of brucellosis in animals was 4.7%.Human brucellosis among the target groups was higher in northwestern Aseer(53.3%)compared to Southeastern Aseer(25.9%)and Jazan region(20.6%).The disease was more prevalent among non-Saudi nationals(35.2%)compared to Saudis(30.5%).The rate of brucellosis among butchers and shepherds was 37.5%and 37.2%,respectively.The rate of brucellosis was 37.8%in people over 30 years of age.Our univariate analysis showed that residing in Aseer region(OR:2.60,95%CI:1.50-4.40),especially residing in northwestern Aseer region(OR:4.40,95%CI:2.40-7.90),frequent consumption of raw meat(OR:2.90,95%CI:1.50-5.50),shepherds(OR:2.10,95%CI:0.80-5.30),owning sheep(OR:2.20,95%CI:1.10-4.40),daily contact with animals(OR:2.10,95%CI:0.75-5.80),and those>30-year-old(OR:1.50,95%CI:1.00-2.40)were significantly associated with increased risks of brucellosis.Our multivariate analysis further showed that residing in northwestern Aseer(OR:9.16,95%CI:3.39-24.76)and having sheep(OR:1.16,95%CI:1.00-1.35)were significant and independent risks of brucellosis while residing in agricultural region(OR:0.28,95%CI:0.10-0.78)was a significant and independent protector against brucellosis.Conclusions:The study concluded that residing in northwestern Aseer area and having animals(sheep)are associated with significantly increased risks of brucellosis.
文摘Background: Periodontitis is the predominant disease in the oral cavity and there are alterations in the periodontal tissues associated with the aging and gender of the patient. Objective: The present study was designed to assess the impact of age and gender of the patient on severity and types of periodontal diseases among patients from two regions in Saudi Arabia. Materials and Methods: The current study was done on 600 Saudi patients from Aseer and Tabuk regions in Saudi Arabia (50% males and 50% females) and divided into three equal groups (n = 200) according to the patients age: children and young patients (1 - 24 years old), adults patients (25 - 64 years old) and seniors patients (more than 65 years old). Plaque index (PLI), gingival index (GI), periodontal pocket depth (PPD) and clinical attachment loss (CAL) were recorded from all participants. The data were collected and analyzed with SPSS to determine the mean and standard deviation (±SD) and the values of significance (P ≤ 0.005). Results: The results of the present study revealed that 340 patients (56.7%) were affected with gingivitis dental biofilm induced and 260 patients were affected by periodontitis. Gingivitis dental biofilm-induced is especially detected in children and young patients (33.3%), adult patients (16.7%) and seniors patients (6.7%) whereas periodontitis cases were seen among the adult and seniors patients (16.7%) and (26.6%) respectively. Furthermore, the present study displayed the higher severity and prevalence of gingivitis dental biofilm-induced and periodontitis among females more than males in group II and group III maybe due to hormonal changes. The present study saw that there are statistically significant differences in clinical findings in the comparison between groups of this study (P ≤ 0.005). Conclusion: We conclude that there is a relation between severity and types of periodontal diseases and the ages and genders of Saudi patients in Aseer and Tabuk regions, Saudi Arabia.
