Arsenic methyltransferase（As3mt） catalyzes the conversion of inorganic arsenic（i As） to its methylated metabolites, including toxic methylarsonite（MAs~Ⅲ） and dimethylarsinite（DMAs~Ⅲ）. Knockout（KO） of As3 m...Arsenic methyltransferase（As3mt） catalyzes the conversion of inorganic arsenic（i As） to its methylated metabolites, including toxic methylarsonite（MAs~Ⅲ） and dimethylarsinite（DMAs~Ⅲ）. Knockout（KO） of As3 mt was shown to reduce the capacity to methylate i As in mice. However, no data are available on the oxidation states of As species in tissues of these mice. Here, we compare the oxidation states of As species in tissues of male C57BL/6 As3mt-KO and wild-type（WT） mice exposed to arsenite（iA s~Ⅲ） in drinking water. WT mice were exposed to50 mg/L As and As3mt-KO mice that cannot tolerate 50 mg/L As were exposed to 0, 15, 20, 25 or30 mg/L As. iA s~Ⅲaccounted for 53% to 74% of total As in liver, pancreas, adipose, lung, heart, and kidney of As3mt-KO mice; tri- and pentavalent methylated arsenicals did not exceed 10% of total As. Tissues of WT mice retained iA s and methylated arsenicals： iA s~Ⅲ, MAs~Ⅲand DMAs~Ⅲ represented 55%‐68% of the total As in the liver, pancreas, and brain. High levels of methylated species, particularly MAs~Ⅲ, were found in the intestine of WT, but not As3mt-KO mice,suggesting that intestinal bacteria are not a major source of methylated As. Blood of WT mice contained significantly higher levels of As than blood of As3mt-KO mice. This study is the first to determine oxidation states of As species in tissues of As3mt-KO mice. Results will help to design studies using WT and As3mt-KO mice to examine the role of iA s methylation in adverse effects of iA s exposure.展开更多
Flow has been widely studied in the field of positive psychology.However,little is known regarding its biological mechanism.This study aimed to ascertain flow-related gene loci.We investigated the association between ...Flow has been widely studied in the field of positive psychology.However,little is known regarding its biological mechanism.This study aimed to ascertain flow-related gene loci.We investigated the association between flow and five single nucleotide polymorphisms associated with common mental disorders among a sample of 870 healthy 1 st year students of Jining Medical University,Shandong Province,China.This study was approved by the Ethics Committee of Jining Medical University(approval number:JNMC-2016-KY-001)on June 1,2016.rs11191454 demonstrated significant statistical association with flow after adjusting for age and gender(P=0.004).The allele carriers achieved higher scores in all 4 dimensions of flow:merging of action and awareness,challenge-skill balance,sense of control,and clear goals.This biological research article indicates that rs11191454 in the arsenite methyltransferase(AS3MT)gene might be associated with flow in a Chinese Han population,and that might result from altered arsenic metabolism.展开更多
基金supported by NIH grant No. 2 R01 ES010845 to M.S.the UNC Nutrition Obesity Research Center grant no. DK056350,+1 种基金NIH grant No. P30ES010126 to the UNC Center for Environmental Health and Susceptibilitysupported by a pre-doctoral traineeship (National Research Service Award T32 ES007126) from the National Institute of Environmental Health Sciences, NIH
文摘Arsenic methyltransferase（As3mt） catalyzes the conversion of inorganic arsenic（i As） to its methylated metabolites, including toxic methylarsonite（MAs~Ⅲ） and dimethylarsinite（DMAs~Ⅲ）. Knockout（KO） of As3 mt was shown to reduce the capacity to methylate i As in mice. However, no data are available on the oxidation states of As species in tissues of these mice. Here, we compare the oxidation states of As species in tissues of male C57BL/6 As3mt-KO and wild-type（WT） mice exposed to arsenite（iA s~Ⅲ） in drinking water. WT mice were exposed to50 mg/L As and As3mt-KO mice that cannot tolerate 50 mg/L As were exposed to 0, 15, 20, 25 or30 mg/L As. iA s~Ⅲaccounted for 53% to 74% of total As in liver, pancreas, adipose, lung, heart, and kidney of As3mt-KO mice; tri- and pentavalent methylated arsenicals did not exceed 10% of total As. Tissues of WT mice retained iA s and methylated arsenicals： iA s~Ⅲ, MAs~Ⅲand DMAs~Ⅲ represented 55%‐68% of the total As in the liver, pancreas, and brain. High levels of methylated species, particularly MAs~Ⅲ, were found in the intestine of WT, but not As3mt-KO mice,suggesting that intestinal bacteria are not a major source of methylated As. Blood of WT mice contained significantly higher levels of As than blood of As3mt-KO mice. This study is the first to determine oxidation states of As species in tissues of As3mt-KO mice. Results will help to design studies using WT and As3mt-KO mice to examine the role of iA s methylation in adverse effects of iA s exposure.
基金approved by the Ethics Committee of Jining Medical University,China(approval number:JNMC-2016-KY-001)on June 1,2016.
文摘Flow has been widely studied in the field of positive psychology.However,little is known regarding its biological mechanism.This study aimed to ascertain flow-related gene loci.We investigated the association between flow and five single nucleotide polymorphisms associated with common mental disorders among a sample of 870 healthy 1 st year students of Jining Medical University,Shandong Province,China.This study was approved by the Ethics Committee of Jining Medical University(approval number:JNMC-2016-KY-001)on June 1,2016.rs11191454 demonstrated significant statistical association with flow after adjusting for age and gender(P=0.004).The allele carriers achieved higher scores in all 4 dimensions of flow:merging of action and awareness,challenge-skill balance,sense of control,and clear goals.This biological research article indicates that rs11191454 in the arsenite methyltransferase(AS3MT)gene might be associated with flow in a Chinese Han population,and that might result from altered arsenic metabolism.
