Introduction: Healthcare practitioners in Côte d’Ivoire have reported low efficacy rates for the Artemether-Lumefantrine (AL) combination in treating uncomplicated malaria. This study aims to compare the therape...Introduction: Healthcare practitioners in Côte d’Ivoire have reported low efficacy rates for the Artemether-Lumefantrine (AL) combination in treating uncomplicated malaria. This study aims to compare the therapeutic responses of two generic formulations of this antimalarial agent, Artéfan® and Coartem®, which have been available in Côte d’Ivoire’s healthcare facilities since 2008. Method: We conducted a comparative analysis of a bi-centric clinical trial in Côte d’Ivoire, evaluating the therapeutic efficacy of Artéfan® in Daloa and Coartem® in Ayamé between 2018 and 2019, following the WHO’s 42-day protocol. The primary evaluation metric was the Adequate Clinical and Parasitological Response (ACPR) at day 42 (D42) post-PCR correction. Results: A total of sixty-two patients were included at each site. The fever clearance time was 24 hours in both Daloa and Ayamé. Parasitic clearance occurred in 48 hours in Daloa and 72 hours in Ayamé. In the per-protocol analysis at D42, the ACPR was 92% in Daloa and 98% in Ayamé, with no significant difference noted (p = 0.1). Conclusion: Artéfan® and Coartem® exhibited statistically equivalent therapeutic efficacy. Nonetheless, the Artemether-Lumefantrine combination warrants further surveillance due to notable re-infestation rates.展开更多
Rabies is caused by infection of rabies virus(RABV)and remains a serious threat to the global public health.Except for the requirement for cold chain and high cost of human rabies immune globulin,no small molecule dru...Rabies is caused by infection of rabies virus(RABV)and remains a serious threat to the global public health.Except for the requirement for cold chain and high cost of human rabies immune globulin,no small molecule drugs are currently available for clinical treatment of rabies.So,it is of great importance to identify novel compounds that can effectively inhibit RABV infection.Artesunate(ART)and dihydroartemisinin(DHA),two derivatives of artemisinin,are widely used for treatment of malaria in adults and children,showing high safety.In this study,we found that both ART and DHA were able to inhibit RABV replication in host cells at a low concentration(0.1μmol/L).The antiviral effects of ART and DHA were independent of viral strains and cell lines.Pre-treatment with ART or DHA for 2 h in vitro did not affect the viral replication in host cells,implying that ART and DHA neither reduced the viability of RABV directly nor inhibited the binding and entrance of the virus to host cells.Further studies revealed that ART and DHA inhibited RABV genomic RNA synthesis and viral gene transcription.Treatment with ART or DHA(5 mg/kg)by intramuscular injection improved,to some extent,the survival rate of RABV-challenged mice.Combination treatment with derivatives of artemisinin and mannitol significantly improved the survival rate of RABV-challenged mice.The results suggest that ART and DHA have a great potential to be explored as new anti-rabies agents for treatment of rabies.展开更多
Purpose： The objective of this study was to investigate the anti-tumor effects and analyze the mechanism of artesunate （ART） action on breast cancer in vivo using tumor transplanted nude mice. Methods： The human b...Purpose： The objective of this study was to investigate the anti-tumor effects and analyze the mechanism of artesunate （ART） action on breast cancer in vivo using tumor transplanted nude mice. Methods： The human breast tumor cell line MCF-7 was transplanted into nude mice, and the animals were treated with various doses of ART alone or in combination with cyclophosphamide （CTX） or normal saline （NS）. The tumor inhibitory effects were observed and compared, and the ultrastructural morphology of the transplanted tumor cells was observed by electron microscopy. The apoptosis rates and cell cycle status were detected by flow cytometry （FCM）. The expression of apoptosis-related proteins p53, Bcl-2, Bax and Caspase-3 were detected by immunohistochemistry and IGF-IR was detected by western blot. The expression correlation for these proteins was also analyzed. Results： The tumor inhibition rates in the low dose ART group, high dose ART group, CTX group and combined drug therapy group were （24.39±10.20）%, （40.24±7.02）%, （57.01±5.84）% and （68.29±5.1）%, respectively. The cell cycle was arrested in phase G0/Gt after treatment with ART. The expression of Bcl-2 was significantly reduced, and the expression levels of Bax and Caspase-3 were significantly increased in the ART group compared to the negative control saline group. There was no significant difference detected in p53 expression. The Bcl-2 level was negatively related to Bax and Caspase-3. The western blotting results showed IGF-IR downregulation. Conclusions： ART inhibits the growth of MCF-7 breast tumor cell xenografts in nude mice. The anti-tumor mechanism of ART for human breast carcinoma in nude mice might be correlated with the alteration of apoptosis related protein expression, which may further induce apoptosis and inhibit cell proliferation.展开更多
One of the keys to the inheritance and utilization of shadow play art in kindergartens is to select valuable, appropriate and classic shadow play art resources. Our garden insists on the identification from the perspe...One of the keys to the inheritance and utilization of shadow play art in kindergartens is to select valuable, appropriate and classic shadow play art resources. Our garden insists on the identification from the perspectives of culture, art, education, regional characteristics and other aspects, carries out the collection and optimization of shadow play art resources, and has established a shadow play art resource bank serving the learning and development of young children;Based on the early childhood development, it explores the educational, appropriate and innovative principles of shadow play art resources applied in early childhood education. By adopting measures such as strengthening teachers culture to lead the curriculum, and enhancing teachers professional level of art education, teachers ability to use shadow play art resources will be enhanced, and a bridge of home-based collaborative education will be built, so that shadow play art can truly live and be inherited.展开更多
Fine art authentication plays a significant role in protecting cultural heritage and ensuring the integrity of artworks.Traditional authentication methods require professionals to collect many reference materials and ...Fine art authentication plays a significant role in protecting cultural heritage and ensuring the integrity of artworks.Traditional authentication methods require professionals to collect many reference materials and conduct detailed analyses.To ease the difficulty,we collaborate with domain experts to develop a GPT-based agent,namely ArtEyer,that offers accurate attributions,determines the origin and authorship,and executes visual analytics.Despite the convenience of the conversational user interface,novice users may still face challenges due to the hallucination issue and the steep learning curve associated with prompting.To face these obstacles,we propose a novel solution that places interactive data visualizations into the conversations.We create contextual visualizations from an external domain-dependent database to ensure data trustworthiness and allow users to provide precise instructions to the agent by interacting directly with these visualizations,thus overcoming the vagueness inherent in natural language-based prompting.We evaluate ArtEyer through an in-lab user study and demonstrate its usage with a real-world case.展开更多
目的探讨抗逆转录病毒治疗(antiretroviral treatment,ART)对HIV-1感染者(people living with HIV-1,PLWH)CD3^(+)T细胞PD-1和CD160表达及其活化凋亡水平的影响。方法收集66例慢性HIV-1感染患者,根据是否接受ART治疗,将PLWH分为non-ART...目的探讨抗逆转录病毒治疗(antiretroviral treatment,ART)对HIV-1感染者(people living with HIV-1,PLWH)CD3^(+)T细胞PD-1和CD160表达及其活化凋亡水平的影响。方法收集66例慢性HIV-1感染患者,根据是否接受ART治疗,将PLWH分为non-ART组(39例)和ART组(27例),并设置HIV-1阴性对照组(30例)。采用流式细胞术检测3组外周血CD4计数及CD3^(+)T细胞中PD-1、CD160、CD95、CD38和HLA-DR的表达,并分析3组间不同指标的差异性。结果non-ART组CD3^(+)T细胞百分比和PD-1^(+)T细胞百分比与ART组相比差异无统计学意义(P=0.8141,P>0.9999),而其CD160^(+)CD3^(+)T细胞百分比显著高于ART组(P=0.0162)。共表达分析显示,non-ART组PD-1^(+)CD160^(+)CD3^(+)T细胞百分比显著高于ART组(P=0.0121),而在单阳的CD3^(+)T性细胞(CD160^(+)PD-1^(-)和PD-1^(+)CD160-)细胞群,ART组和non-ART组差异均无统计学意义(P=0.3382,P>0.9999)。在PD-1^(+)CD160^(+)CD3^(+)T细胞CD38方面,non-ART组显著高于ART组和HIV-1阴性对照组(P=0.0005,P=0.0015),HIV-1阴性对照组和ART组差异无统计学意义(P>0.9999)。结论ART对PLWH中PD-1^(+)CD160^(+)CD3^(+)T细胞百分比及其CD38的表达具有调节作用。展开更多
本文探讨Art to Wear即“可以穿的艺术”,其中大部分作品为服装,少量作品是帽子和首饰。通过分析Art to Wear代表艺术家,解读Art to Wear艺术作品,从而探讨美国20世纪60年代到80年代女性艺术家群体如何以艺术为媒介对自身经历进行叙述...本文探讨Art to Wear即“可以穿的艺术”,其中大部分作品为服装,少量作品是帽子和首饰。通过分析Art to Wear代表艺术家,解读Art to Wear艺术作品,从而探讨美国20世纪60年代到80年代女性艺术家群体如何以艺术为媒介对自身经历进行叙述性表现。60年代末70年代初处于美国女性主义的第二次浪潮,众多美国名校的年轻白人女大学生,不断为女性的参政、就业、医疗、最低工资等争取更多的权利,而到了80年代遭到美国保守主义的强烈打击,追求自由平等的女性主义运动跌入低谷。第二次女性主义运动的跌宕正是Art to Wear艺术运动的发展时期,艺术学院在读青年女生们发起Art to Wear艺术运动,她们在保守主义与激进主义的影响下寻求精神、生活的平衡,极力反叛女性束身塑形来博得男性视觉愉悦、通过同性的嫉妒来肯定自身价值。她们对家庭、事业、社会地位开始深思,通过作品叙述对社会性别与身份认定的迷茫,表达保守与激进思想的内心纠结。展开更多
With the progress of urbanization,urban landscape design has garnered increasing attention.Urban landscape design can not only reflect the construction level of a city but also has the functions of beautifying the urb...With the progress of urbanization,urban landscape design has garnered increasing attention.Urban landscape design can not only reflect the construction level of a city but also has the functions of beautifying the urban environment and enhancing the city image.Modern art enriches the expression forms of urban landscape design,and its integration with urban landscape design can greatly improve the urban landscape.This article elaborated on the connotations of modern art and urban landscape design and analyzed the application of modern art in urban landscape design.The application of modern art can help to achieve the goal of urban landscape design to the maximum extent.展开更多
文摘Introduction: Healthcare practitioners in Côte d’Ivoire have reported low efficacy rates for the Artemether-Lumefantrine (AL) combination in treating uncomplicated malaria. This study aims to compare the therapeutic responses of two generic formulations of this antimalarial agent, Artéfan® and Coartem®, which have been available in Côte d’Ivoire’s healthcare facilities since 2008. Method: We conducted a comparative analysis of a bi-centric clinical trial in Côte d’Ivoire, evaluating the therapeutic efficacy of Artéfan® in Daloa and Coartem® in Ayamé between 2018 and 2019, following the WHO’s 42-day protocol. The primary evaluation metric was the Adequate Clinical and Parasitological Response (ACPR) at day 42 (D42) post-PCR correction. Results: A total of sixty-two patients were included at each site. The fever clearance time was 24 hours in both Daloa and Ayamé. Parasitic clearance occurred in 48 hours in Daloa and 72 hours in Ayamé. In the per-protocol analysis at D42, the ACPR was 92% in Daloa and 98% in Ayamé, with no significant difference noted (p = 0.1). Conclusion: Artéfan® and Coartem® exhibited statistically equivalent therapeutic efficacy. Nonetheless, the Artemether-Lumefantrine combination warrants further surveillance due to notable re-infestation rates.
基金We thank Dr.Xianzhu Xia(Academy of Military Medical Sciences)for providing the CVS-11 virus.We thank Dr.Zhenfang Fu(Huazhong Agricultural University)for providing the CVS-24 virus.This work was supported by the National Key Research and Development Program of China(2016YFD0500400)National Natural Science Foundation of China(31772742)Natural Science Foundation of Guangdong(2015A03031103).
文摘Rabies is caused by infection of rabies virus(RABV)and remains a serious threat to the global public health.Except for the requirement for cold chain and high cost of human rabies immune globulin,no small molecule drugs are currently available for clinical treatment of rabies.So,it is of great importance to identify novel compounds that can effectively inhibit RABV infection.Artesunate(ART)and dihydroartemisinin(DHA),two derivatives of artemisinin,are widely used for treatment of malaria in adults and children,showing high safety.In this study,we found that both ART and DHA were able to inhibit RABV replication in host cells at a low concentration(0.1μmol/L).The antiviral effects of ART and DHA were independent of viral strains and cell lines.Pre-treatment with ART or DHA for 2 h in vitro did not affect the viral replication in host cells,implying that ART and DHA neither reduced the viability of RABV directly nor inhibited the binding and entrance of the virus to host cells.Further studies revealed that ART and DHA inhibited RABV genomic RNA synthesis and viral gene transcription.Treatment with ART or DHA(5 mg/kg)by intramuscular injection improved,to some extent,the survival rate of RABV-challenged mice.Combination treatment with derivatives of artemisinin and mannitol significantly improved the survival rate of RABV-challenged mice.The results suggest that ART and DHA have a great potential to be explored as new anti-rabies agents for treatment of rabies.
基金Province Science Fund for Young Scholars (No. QC05C46)Science Foundation from Health Bureau of Heilongjiang Province (No. 2005-47)
文摘Purpose： The objective of this study was to investigate the anti-tumor effects and analyze the mechanism of artesunate （ART） action on breast cancer in vivo using tumor transplanted nude mice. Methods： The human breast tumor cell line MCF-7 was transplanted into nude mice, and the animals were treated with various doses of ART alone or in combination with cyclophosphamide （CTX） or normal saline （NS）. The tumor inhibitory effects were observed and compared, and the ultrastructural morphology of the transplanted tumor cells was observed by electron microscopy. The apoptosis rates and cell cycle status were detected by flow cytometry （FCM）. The expression of apoptosis-related proteins p53, Bcl-2, Bax and Caspase-3 were detected by immunohistochemistry and IGF-IR was detected by western blot. The expression correlation for these proteins was also analyzed. Results： The tumor inhibition rates in the low dose ART group, high dose ART group, CTX group and combined drug therapy group were （24.39±10.20）%, （40.24±7.02）%, （57.01±5.84）% and （68.29±5.1）%, respectively. The cell cycle was arrested in phase G0/Gt after treatment with ART. The expression of Bcl-2 was significantly reduced, and the expression levels of Bax and Caspase-3 were significantly increased in the ART group compared to the negative control saline group. There was no significant difference detected in p53 expression. The Bcl-2 level was negatively related to Bax and Caspase-3. The western blotting results showed IGF-IR downregulation. Conclusions： ART inhibits the growth of MCF-7 breast tumor cell xenografts in nude mice. The anti-tumor mechanism of ART for human breast carcinoma in nude mice might be correlated with the alteration of apoptosis related protein expression, which may further induce apoptosis and inhibit cell proliferation.
文摘One of the keys to the inheritance and utilization of shadow play art in kindergartens is to select valuable, appropriate and classic shadow play art resources. Our garden insists on the identification from the perspectives of culture, art, education, regional characteristics and other aspects, carries out the collection and optimization of shadow play art resources, and has established a shadow play art resource bank serving the learning and development of young children;Based on the early childhood development, it explores the educational, appropriate and innovative principles of shadow play art resources applied in early childhood education. By adopting measures such as strengthening teachers culture to lead the curriculum, and enhancing teachers professional level of art education, teachers ability to use shadow play art resources will be enhanced, and a bridge of home-based collaborative education will be built, so that shadow play art can truly live and be inherited.
基金This document contains the results of the research project funded by the National Social Science Fund of China (19ZDA046)NSF of China (62302440,U22A2032)+1 种基金China Postdoctoral Science Foundation (2023TQ0288)the Fundamental Research Funds for the Central Universities,China.
文摘Fine art authentication plays a significant role in protecting cultural heritage and ensuring the integrity of artworks.Traditional authentication methods require professionals to collect many reference materials and conduct detailed analyses.To ease the difficulty,we collaborate with domain experts to develop a GPT-based agent,namely ArtEyer,that offers accurate attributions,determines the origin and authorship,and executes visual analytics.Despite the convenience of the conversational user interface,novice users may still face challenges due to the hallucination issue and the steep learning curve associated with prompting.To face these obstacles,we propose a novel solution that places interactive data visualizations into the conversations.We create contextual visualizations from an external domain-dependent database to ensure data trustworthiness and allow users to provide precise instructions to the agent by interacting directly with these visualizations,thus overcoming the vagueness inherent in natural language-based prompting.We evaluate ArtEyer through an in-lab user study and demonstrate its usage with a real-world case.
文摘目的探讨抗逆转录病毒治疗(antiretroviral treatment,ART)对HIV-1感染者(people living with HIV-1,PLWH)CD3^(+)T细胞PD-1和CD160表达及其活化凋亡水平的影响。方法收集66例慢性HIV-1感染患者,根据是否接受ART治疗,将PLWH分为non-ART组(39例)和ART组(27例),并设置HIV-1阴性对照组(30例)。采用流式细胞术检测3组外周血CD4计数及CD3^(+)T细胞中PD-1、CD160、CD95、CD38和HLA-DR的表达,并分析3组间不同指标的差异性。结果non-ART组CD3^(+)T细胞百分比和PD-1^(+)T细胞百分比与ART组相比差异无统计学意义(P=0.8141,P>0.9999),而其CD160^(+)CD3^(+)T细胞百分比显著高于ART组(P=0.0162)。共表达分析显示,non-ART组PD-1^(+)CD160^(+)CD3^(+)T细胞百分比显著高于ART组(P=0.0121),而在单阳的CD3^(+)T性细胞(CD160^(+)PD-1^(-)和PD-1^(+)CD160-)细胞群,ART组和non-ART组差异均无统计学意义(P=0.3382,P>0.9999)。在PD-1^(+)CD160^(+)CD3^(+)T细胞CD38方面,non-ART组显著高于ART组和HIV-1阴性对照组(P=0.0005,P=0.0015),HIV-1阴性对照组和ART组差异无统计学意义(P>0.9999)。结论ART对PLWH中PD-1^(+)CD160^(+)CD3^(+)T细胞百分比及其CD38的表达具有调节作用。
文摘本文探讨Art to Wear即“可以穿的艺术”,其中大部分作品为服装,少量作品是帽子和首饰。通过分析Art to Wear代表艺术家,解读Art to Wear艺术作品,从而探讨美国20世纪60年代到80年代女性艺术家群体如何以艺术为媒介对自身经历进行叙述性表现。60年代末70年代初处于美国女性主义的第二次浪潮,众多美国名校的年轻白人女大学生,不断为女性的参政、就业、医疗、最低工资等争取更多的权利,而到了80年代遭到美国保守主义的强烈打击,追求自由平等的女性主义运动跌入低谷。第二次女性主义运动的跌宕正是Art to Wear艺术运动的发展时期,艺术学院在读青年女生们发起Art to Wear艺术运动,她们在保守主义与激进主义的影响下寻求精神、生活的平衡,极力反叛女性束身塑形来博得男性视觉愉悦、通过同性的嫉妒来肯定自身价值。她们对家庭、事业、社会地位开始深思,通过作品叙述对社会性别与身份认定的迷茫,表达保守与激进思想的内心纠结。
文摘With the progress of urbanization,urban landscape design has garnered increasing attention.Urban landscape design can not only reflect the construction level of a city but also has the functions of beautifying the urban environment and enhancing the city image.Modern art enriches the expression forms of urban landscape design,and its integration with urban landscape design can greatly improve the urban landscape.This article elaborated on the connotations of modern art and urban landscape design and analyzed the application of modern art in urban landscape design.The application of modern art can help to achieve the goal of urban landscape design to the maximum extent.
