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基于AHP-RCA根源分析法的煤矿瓦斯爆炸事故风险评价研究
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作者 张景钢 刘鹏飞 +1 位作者 何鑫 孙静雯 《矿业安全与环保》 北大核心 2026年第1期109-115,共7页
为预防煤矿瓦斯爆炸事故的发生,需确定事故危险源,分析事故发生的根源,并对其致因加以控制。探究RCA(根源分析法)在煤矿瓦斯爆炸事故分析中的优势、实施过程,引入AHP-RCA分析法,并结合实际案例运用头脑风暴、鱼骨树等分析工具,分析煤矿... 为预防煤矿瓦斯爆炸事故的发生,需确定事故危险源,分析事故发生的根源,并对其致因加以控制。探究RCA(根源分析法)在煤矿瓦斯爆炸事故分析中的优势、实施过程,引入AHP-RCA分析法,并结合实际案例运用头脑风暴、鱼骨树等分析工具,分析煤矿瓦斯爆炸事故发生的规律和共性问题,排查事故成因;在此基础上运用AHP(层次分析法)构造煤矿瓦斯爆炸事故成因层次模型,通过构造判断矩阵对事故成因进行定量分析,确定各判断矩阵的特征向量与权重,得出造成瓦斯爆炸事故的主要成因。研究结果表明:AHP-RCA分析法突破了传统事故树分析的局限性,通过权重量化可实现致因要素的优先级排序,其中人为因素与管理因素为主要致灾因子,基于此对在岗人员的安全管理提出整改措施和建议,可更好地预防事故发生。 展开更多
关键词 瓦斯爆炸事故 rca AHP AHP-rca 判断矩阵
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Noninvasive interception of circulating leukocyte in vivo by optical tweezer array 被引量:1
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作者 Sisi Ge Jinhua Zhou Xunbin Wei 《Journal of Innovative Optical Health Sciences》 2026年第1期56-69,共14页
The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly a... The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly applicable optical trapping system based on a custom-designed 2×3 optical tweezer array,which enables the real-time interception and manipulation of circulating leukocytes in live animals.By utilizing intrinsic velocity differences between leukocytes and red blood cells,the system achieves stable trapping of individual leukocytes in vessels 15-20μm in diameter and decelerates multiple cells in vessels greater than 20μm.Notably,it also enables the optical blockage of lymphatic vessels exceeding 50μm,a previously unreported capability.This label-free,noninvasive approach operates without repeated blood draws and is compatible with diverse vessel geometries and flow dynamics.The system offers a generalizable solution for in vivo cell extraction and analysis,paving the way for high-precision single-cell technologies in biomedical research and clinical translation. 展开更多
关键词 Leukocytes optical tweezer array in vivo interception
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Deep learning-based number of sources estimation under colored noise and imperfect array
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作者 Linqiang JIANG Tao TANG +2 位作者 Zhidong WU Ding WANG Paihang ZHAO 《Chinese Journal of Aeronautics》 2026年第2期414-428,共15页
The estimation of the Number of Sources(NoS)is a significant challenge in signal processing,particularly due to the impact of colored noise on the performance of NoS estimation.This paper proposes a Multidimensional F... The estimation of the Number of Sources(NoS)is a significant challenge in signal processing,particularly due to the impact of colored noise on the performance of NoS estimation.This paper proposes a Multidimensional Feature Network(MFNet)which is designed for NoS estimation by extracting features of the sampled received signals and Sampled Covariance Matrix(SCM).The MFNet treats the raw signal and the SCM as two different types of data,and is able to achieve NoS estimation under colored noise and imperfect array.MFNet employs the Gated Recurrent Unit(GRU)to capture sequential information from the original signal data and to construct the Pseudo Covariance Matrix(PCM).Subsequently,various dimensional features,including eigenvalues and the Gerschgorin disk radius,are extracted from both the PCM and SCM,which are then jointly input into the subsequent network.An overall accuracy of 82%can be achieved after network training.The ablation experimental results demonstrate the effectiveness of multiple inputs.And simulation results demonstrate that the proposed MFNet achieves higher estimation accuracy compared to existing algorithms and exhibits greater robustness against colored noise. 展开更多
关键词 Number of source estimation Deep learning Colored noise Imperfect array array signal processing
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A new 10K liquid SNP genotyping array for wax gourd and its application in heterosis utilization and cultivars identification
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作者 Dan Liu Lingling Xie +4 位作者 Yuting Lei Bingchuan Tian Daolong Liao Fangfang Wu Baobin Mi 《Journal of Integrative Agriculture》 2026年第2期734-743,共10页
High-throughput single nucleotide polymorphism(SNP) arrays have emerged as essential genotyping tools,significantly accelerating breeding programs and advancing basic research.In this study,a high-throughput 10K SNP g... High-throughput single nucleotide polymorphism(SNP) arrays have emerged as essential genotyping tools,significantly accelerating breeding programs and advancing basic research.In this study,a high-throughput 10K SNP genotyping array for wax gourd was developed using genotyping by target sequencing(GBTS),featuring 10,722 SNPs evenly distributed across all 12 chromosomes,including 278 functional loci associated with key economic traits.To demonstrate its utility,genetic distances among 19 elite inbred lines were calculated from SNP data and correlated with heterosis for single fruit weight.The results revealed that greater genetic distance was associated with higher middle parent heterosis(MPH) for single fruit weight.Furthermore,56 commercial wax gourd cultivars collected from eight regions were selected and genotyped.Population structure analysis,phylogenetic analysis,and principal component analysis(PCA) collectively indicated that these cultivars fall into two major groups.Group I,comprising black or dark green skinned wax gourds,exhibited lower genetic diversity than Group II,which includes green or light green skinned varieties,reflecting shorter genetic distances within Group I.Finally,60 polymorphic SNPs were used to construct DNA fingerprints for distinguishing the 56 cultivars.As the first high-throughput genotyping platform for wax gourd,this SNP array provides an effective and powerful tool for genetic analysis. 展开更多
关键词 wax gourd SNP genotyping array HETEROSIS cultivar identification DNA fingerprint
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Cellular behavior regulation and adhesion force measurement based on flexible micropillar arrays
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作者 Ruoyu Feng Hang Qi +1 位作者 Xuexin Duan Yanyan Wang 《Nanotechnology and Precision Engineering》 2026年第1期34-43,共10页
Systematic understanding of the interaction between cells and their microenvironment is of wide interest.To investigate this interaction,a flexible micropillar array device integrating dual functions of cell behavior ... Systematic understanding of the interaction between cells and their microenvironment is of wide interest.To investigate this interaction,a flexible micropillar array device integrating dual functions of cell behavior regulation and adhesion measurement is developed.Micropillar arrays with high and low densities are designed to explore the role of substrate topography in the behavior of human bone marrow mesenchymal stem cells.In addition,a method is established for quantifying weak cell adhesion forces on the basis of micropillar deflections.The results show that cell cytoplasmic adhesion is greater on a low-density micropillar array than that on a high-density array and is localized mainly in the perinuclear region of the cytoplasm rather than in pseudopods.It is also found that the micropillar array topography facilitates the oriented spreading of cell morphology and pseudopod formation,and a reduction in focal adhesion aggregation and F-actin polarization compared with a flat substrate.Notably,cells cultured on a low-density micropillar array exhibited a higher number of pseudopods,stronger adhesion forces,and greater stiffness compared with those on a high-density array.In summary,this work employs an adhesion force sensor,immunofluorescence staining,and atomic force microscopy to investigate the mechanical properties of cells and elucidate the mechanisms by which micropillar topographical cues regulate the adhesion of mesenchymal stem cells to the substrate.The micropillar array force sensor developed in this study provides an effective tool for simultaneously modulating cell behavior and quantifying adhesion forces,offering valuable insights for biomechanical research. 展开更多
关键词 Micropillar array Mesenchymal stem cell Cell mechanics Adhesion force
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Yosemay金枪鱼 AG-04纯银RCA信号线、AG-03纯银电源线
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《视听前线》 2026年第1期45-45,共1页
特点。金枪鱼所有的线材产品制造过程都在金枪鱼YOSEMAY自家工厂内,使用金属模具上精密注塑机一体成型生产。RCA信号线AG-04采用多芯线纯银导体,不仅用上了纯银导体,就连屏蔽也使用镀银屏蔽网。线芯导体的绝缘层,使用薄薄一层铁氟龙,达... 特点。金枪鱼所有的线材产品制造过程都在金枪鱼YOSEMAY自家工厂内,使用金属模具上精密注塑机一体成型生产。RCA信号线AG-04采用多芯线纯银导体,不仅用上了纯银导体,就连屏蔽也使用镀银屏蔽网。线芯导体的绝缘层,使用薄薄一层铁氟龙,达到绝缘效果之余,还有理想的介电系数,更因为够薄,所以保有线身的柔软度。屏蔽结构是铁氟龙+镀银铜编织网+FRPE,插头外壳为纯铜CNC镀金适配碳纤维,自锁式RCA端子主体及接头端子纯铜镀金。 展开更多
关键词 纯银 rca信号线 金枪鱼 镀银屏蔽网 AG-04
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A Reconfigurable Omnidirectional Triboelectric Whisker Sensor Array for Versatile Human–Machine–Environment Interaction
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作者 Weichen Wang Jiaqi Zhu +9 位作者 Hongfa Zhao Fei Yao Yuzhu Zhang Xiankuan Qian Mingrui Shu Zhigang Wu Minyi Xu Hongya Geng Wenbo Ding Juntian Qu 《Nano-Micro Letters》 2026年第3期121-140,共20页
Developing effective,versatile,and high-precision sensing interfaces remains a crucial challenge in human-machine-environment interaction applications.Despite progress in interaction-oriented sensing skins,limitations... Developing effective,versatile,and high-precision sensing interfaces remains a crucial challenge in human-machine-environment interaction applications.Despite progress in interaction-oriented sensing skins,limitations remain in unit-level reconfiguration,multiaxial force and motion sensing,and robust operation across dynamically changing or irregular surfaces.Herein,we develop a reconfigurable omnidirectional triboelectric whisker sensor array(RO-TWSA)comprising multiple sensing units that integrate a triboelectric whisker structure(TWS)with an untethered hydro-sealing vacuum sucker(UHSVS),enabling reversibly portable deployment and omnidirectional perception across diverse surfaces.Using a simple dual-triangular electrode layout paired with MXene/silicone nanocomposite dielectric layer,the sensor unit achieves precise omnidirectional force and motion sensing with a detection threshold as low as 0.024 N and an angular resolution of 5°,while the UHSVS provides reliable and reversible multi-surface anchoring for the sensor units by involving a newly designed hydrogel combining high mechanical robustness and superior water absorption.Extensive experiments demonstrate the effectiveness of RO-TWSA across various interactive scenarios,including teleoperation,tactile diagnostics,and robotic autonomous exploration.Overall,RO-TWSA presents a versatile and high-resolution tactile interface,offering new avenues for intelligent perception and interaction in complex real-world environments. 展开更多
关键词 Reconfigurable sensor array Interaction interface Tactile perception Omnidirectional sensor Reversible anchoring
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Design and investigation of iontronic flexible pressure sensor based on microstructure array
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作者 Zhenzhen Shang Hairong Kou +2 位作者 Libo Yang Xiaoyong Zhang Yuhang Pang 《Nanotechnology and Precision Engineering》 2026年第1期44-51,共8页
Flexible pressure sensors are widely used in fields such as healthcare,owing to their simple preparation method,abundant raw materials,and high sensitivity.To solve the problems of low sensitivity and high noise impac... Flexible pressure sensors are widely used in fields such as healthcare,owing to their simple preparation method,abundant raw materials,and high sensitivity.To solve the problems of low sensitivity and high noise impact that arise with traditional capacitive pressure sensors,this paper proposes an electrical double layer microarray capacitive pressure sensor using silver nanowires(AgNW)and ionic liquids.AgNW/polyimide(PI)flexible electrodes and microarray-structured ionic liquids are utilized to obtain highly sensitive responses to changes in weak physiological signals.The microarray structure is a 20×20 quadrangular prism convex array.Experimental tests show that the sensor has high sensitivity(3.1202 kPa^(−1),0–800 kPa)and fast response/recovery times(100/100 ms).By flexibly attaching the sensor to the skin or a computer mouse,rapid and stable monitoring of micromovements such as facial muscles,vocalization,swallowing,finger joint activity,and mouse clicks can be achieved.The results obtained here are of value for the development of intelligent flexible electronics. 展开更多
关键词 Electronic skin Iontronic mechanism Flexible sensor Ionic dielectric layer Highly sensitive array
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Bio-inspired offset array design for enhanced range in underwater active electrosensing with neural network-based localization
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作者 Meijiang Hou Jiegang Peng +2 位作者 Minan Yang Taoyu Jiang Yang Chen 《Defence Technology(防务技术)》 2026年第3期217-245,共29页
Addressing the critical detection range limitation in active electrosensing(AES)for underwater sensing,this study proposes an enhanced AES system via novel array optimization.While AES offers advantages like interfere... Addressing the critical detection range limitation in active electrosensing(AES)for underwater sensing,this study proposes an enhanced AES system via novel array optimization.While AES offers advantages like interference immunity,acoustic stealth detection,and low cost,its short range restricts applicability.A target perturbation model under differential signal acquisition reveals that signal strength increases with local electric field intensity,target size,differential channel spacing,and conductivity contrast,but decreases with target-electrode distance.To extend detection,novel array configurations were explored.Simulations demonstrate that both rectangular and offset arrays significantly outperform the traditional collinear layout.Specifically,an offset array(with 8 m transmitting–receiving spacing)achieved an effective detection range enhancement exceeding 83%under the same distortion threshold while maintaining simplified electrode structure.Experimental validation confirmed a 100%increase in maximum detection distance to 5 m under identical noise thresholds compared to the collinear array.Furthermore,a fully connected neural network-based localization model achieved a mean positioning error of 14.12 cm at 3.15 m in static scenarios.In dynamic scenarios within 1–3 m,mean errors were controlled between 13.19 cm and 27.56 cm.Mechanistic analysis indicates that increasing the array baseline enhances the signal-to-noise ratio by simultaneously suppressing near-field environmental noise and amplifying far-field signal reception.Structural innovations in array design enabled this study to significantly expand the detection range of AES systems without compromising cost efficiency.These advancements directly promote the engineering application of AES technology,offering critical technical support for underwater defense security monitoring,long-range early warning systems,and maritime rights protection. 展开更多
关键词 Active electrical sensing Target perturbation model array optimization Detection range Fuly connected neural network
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Adaptive implementation of multi-branch convolution with fusion coefficients based on reconfigurable array
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作者 Liu Dongyue Jiang Lin +2 位作者 Wang Mei Li Yuancheng Hao Juan 《High Technology Letters》 2026年第1期39-48,共10页
Reconfigurable array architecture has become an important hardware platform for edge-side deployment of convolutional neural networks due to their high parallelism and flexible programmability.However,traditional mult... Reconfigurable array architecture has become an important hardware platform for edge-side deployment of convolutional neural networks due to their high parallelism and flexible programmability.However,traditional multi-branch convolutional networks suffer from computational redundancy,high memory access overhead,and inefficient branch fusion.Therefore,this paper proposes an adaptive multi-branch convolutional module(AMBC)that integrates software-hardware co-optimization.During training,the learnable fusion coefficients are introduced to enable adaptive fusion of multi-scale features,while in the inference phase,the multiple branches and their normalization parameters are merged with the fusion coefficients into a single 3×3 convolutional kernel through operator fusion.On the SIREA-288 reconfigurable platform,compared with unoptimized multi-branch networks,the proposed AMBC reduces external memory accesses by 47.91%and inference latency by 47.20%,achieving a 1.90×speedup.This approach maximizes the utilization of the reconfigurable logic while minimizing both reconfiguration and data-movement overheads in edge inference. 展开更多
关键词 reconfigurable array processor structural re-parameterization model compression fusion coefficients edge-side inference acceleration hardware-software co-optimization
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3D printed high-temperature ceramic conformal array antenna:Design,analysis,manufacturing,and testing
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作者 Peng Li Ruibo Li +5 位作者 Zijiao Fan Jiujiu Han Guangda Ding Qunbiao Wang Wanye Xu Paolo Rocca 《Defence Technology(防务技术)》 2026年第1期340-353,共14页
In this study,the design,analysis,manufacturing,and testing of a 3D-printed conformal microstrip array antenna for high-temperature environments is presented.3D printing technology is used to fabricate a curved cerami... In this study,the design,analysis,manufacturing,and testing of a 3D-printed conformal microstrip array antenna for high-temperature environments is presented.3D printing technology is used to fabricate a curved ceramic substrate,and laser sintering and microdroplet spraying processes are used to add the conductive metal on the curved substrate.The problems of gain loss,bandwidth reduction,and frequency shift caused by high temperatures are addressed by using a proper antenna design,with parasitic patches,slots,and metal resonant cavities.The antenna prototype is characterized by the curved substrates and the conductive metals for the power dividers,the patch,and the ground plane;its performance is examined up to a temperature of 600℃in a muffle furnace and compared with the results from the numerical analysis.The results show that the antenna can effectively function at 600℃and even higher temperatures. 展开更多
关键词 Ceramic antenna Conformal array High-temperature environment 3D printing High gain and wide band
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Short-period dense linear array ambient noise tomography of shallow crustal structure across the Haichenghe fault zone,China
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作者 Mingruo Jiao Liang Wang +9 位作者 Hongyu Ni Haiyan Wang Lanshu Bai Shuo Liu Zhihong Zhang Zhenpeng Yang Zhengdong Cui Qingshan Sun Rui Qian Mengying Li 《Earthquake Science》 2026年第2期220-234,共15页
The Haicheng region,Liaoning,China,likely hosts a conjugate fault system comprising the NW-trending Haichenghe fault and NE-trending secondary faults.On February 4,1975,at 19:36 CST,an earthquake of M_(S)7.3 and inten... The Haicheng region,Liaoning,China,likely hosts a conjugate fault system comprising the NW-trending Haichenghe fault and NE-trending secondary faults.On February 4,1975,at 19:36 CST,an earthquake of M_(S)7.3 and intensity(MMI)IX hit the city of Haicheng,Liaoning,China.Although deep seismic profiling was previously conducted along the Haichenghe fault,the limited horizontal resolution in the shallow part prevented the recognition of kilometer-scale anomalies.The velocity structure characteristics of the Haichenghe fault and its NE-trending conjugate faults remain unclear.Using the extended range phase shift method,the high-resolution S-wave velocity structures are obtained by deploying a long,dense linear array of 55 short-period seismometers across the fault and NE-trending conjugate faults.The array length was 32 km and inter-station spacing was approximately 600 m,facilitating the collection of approximately 22 days of continuous waveform data.Employing the Extended Range Phase Shift(ERPS)method enabled the extraction of broadband 0.2–5 s Rayleigh wave phase velocity dispersion curves.The broadband dispersion data were used for inversion of the high-resolution S-wave velocity structure to a depth of 8 km from the surface.The velocity structure characteristics and seismicity of the Haichenghe fault and NE-trending conjugate faults were analyzed and compared with nearby fault gas measurements.Results show(1)shallow S-wave velocities show a low-high-low horizontal distribution,corresponding to basin-uplift-basin topography;(2)significant velocity contrasts occur across the Haichenghe fault:its SW segment(0–17 km)exhibits high velocities consistent with Paleoproterozoic crystalline basement(Pt_(1)),while the NE segment(17–32 km)shows low velocities related to Yanshanian intrusions(γ_(5))and Quaternary sediments.NE-trending conjugate faults display sharp velocity gradients marking fracture locations,with all faults being near-vertical to~8 km depth;(3)seismicity at 1–6 km depth mainly clusters in high-velocity zones;at 6–8 km depth,it concentrates beneath the Haichenghe fault in low-velocity areas and along NE-trending faults;(4)the seismic activity characteristics and fault zone width of the Haicheng he fault reflected by velocity imaging results are basically consistent with those obtained by the fault gas measurement method. 展开更多
关键词 dense linear array ambient noise tomography extended range phase shift method S-wave velocity structure Haichenghe fault
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基于RCA方法优化儿童专科医院红外耳温计的临床应用管理
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作者 吴胜 郑彩仙 +2 位作者 王琳 沈云明 郑焜 《中国医学工程》 2025年第6期1-5,共5页
目的探讨根本原因分析法(RCA)在优化儿童专科医院红外耳温计的临床应用管理研究。方法选取浙江大学医学院附属儿童医院2023年新购入且经计量检测合格的122台红外耳温计作为研究对象,按照随机数字表法将其分为对照组与实验组,每组61台。... 目的探讨根本原因分析法(RCA)在优化儿童专科医院红外耳温计的临床应用管理研究。方法选取浙江大学医学院附属儿童医院2023年新购入且经计量检测合格的122台红外耳温计作为研究对象,按照随机数字表法将其分为对照组与实验组,每组61台。对照组采用常规管理手段,实验组开展基于RCA的临床应用管理,计算两组设备的计量合格率及每百台设备的故障次数以比较两组的管理效果。结果对照组的计量合格率为73.77%,明显低于实验组的90.16%(P<0.05);对照组每百台设备的故障次数为(42.89±8.61)次,明显高于实验组的(28.96±6.42)次(P<0.05)。结论采用RCA方法可有效提高红外耳温计的管理水平,降低其故障率、延长使用寿命,值得推广和应用。 展开更多
关键词 根本原因分析法 儿童专科医院 红外耳温计 临床使用管理
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新文科语境下的设计创新教育:RCA跨学科策略分析
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作者 刘月林 宋立巍 《设计》 2025年第19期100-103,共4页
在新文科语境下实现设计创新教育转型,以提升新时代设计创新人才培养质量。借鉴英国皇家艺术学院的跨学科设计教育策略,分析了RCA跨学科设计创新教育的发展脉络、应用策略和教育实践,以及生成式AI发展所引发的设计教育新议题。提出从设... 在新文科语境下实现设计创新教育转型,以提升新时代设计创新人才培养质量。借鉴英国皇家艺术学院的跨学科设计教育策略,分析了RCA跨学科设计创新教育的发展脉络、应用策略和教育实践,以及生成式AI发展所引发的设计教育新议题。提出从设计知识体系建构、理论思维能力培养、提升设计全球胜任力和提升以人为本的AI设计素养4个方面优化设计创新教育。为设计创新教育所面临的关键共性问题提供启发性思路。 展开更多
关键词 新文科 设计创新教育 皇家艺术学院 跨学科 生成式AI
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In Situ Growth of 3D Hierarchical ZnO@Ni_xCo_(1-x)(OH)_y Core/Shell Nanowire/Nanosheet Arrays on Ni Foam for High-Performance Aqueous Hybrid Supercapacitors 被引量:1
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作者 Fumin Wang Mengchao Liu +2 位作者 Xubin Zhang Guojun Lv Mingshuai Sun 《Transactions of Tianjin University》 EI CAS 2018年第3期201-211,共11页
In this study, we designed and synthesized a novel battery-type electrode featuring three-dimensional(3D) hierarchical ZnO@Ni_xCo_(1-x)(OH)_y core/shell nanowire/nanosheet arrays arranged on Nifoam substrate via a two... In this study, we designed and synthesized a novel battery-type electrode featuring three-dimensional(3D) hierarchical ZnO@Ni_xCo_(1-x)(OH)_y core/shell nanowire/nanosheet arrays arranged on Nifoam substrate via a two-step protocol including a wet chemical process followed by electro-deposition. We then characterized its composition, structure and surface morphology by X-ray diff raction, energy-dispersive X-ray spectrometry(EDS), X-ray photoelectron spectroscopy, scanning electron microscopy(SEM), transmission electron microscopy, EDS elemental mapping. Our electrochemical measurements show that the ZnO@Ni_(0.67)Co_(0.33)(OH)_y electrode material exhibited a noticeably high specific capacity of as much as 255(mA ·h)/g at 1 A/g. Additionally, it demonstrated a superior rate capability, as well as an excellent cycling stability with 81.6% capacity retention over 2000 cycles at 5 A/g. This sample delivered a high energy density of 64 W·h/kg and a power density of 250 W/kg at a current density of 1 A/g. With such remarkable electrochemical properties, we expect the 3D hierarchical hybrid electrode material presented in this work to have promising applications for the next generation of energy storage systems. 展开更多
关键词 ZnO@NixCo1-x(OH)y Core/shell nanowire/nanosheet array Supercapacitor Ni foam
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基于CRISPR-Cas12a系统-RCA反应检测重金属汞离子方法的可行性研究
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作者 张玉梅 李德月 江磊 《工业微生物》 2025年第2期76-78,共3页
文章旨在开发一种基于CRISPR-Cas12a系统的高灵敏度和高特异性的重金属汞离子荧光检测方法,以克服现有检测技术耗时长、成本高、灵敏度和特异性差等不足。该方法结合RCA等温扩增技术和CRISPR-Cas12a系统,利用T4 DNA连接酶和phi29 DNA聚... 文章旨在开发一种基于CRISPR-Cas12a系统的高灵敏度和高特异性的重金属汞离子荧光检测方法,以克服现有检测技术耗时长、成本高、灵敏度和特异性差等不足。该方法结合RCA等温扩增技术和CRISPR-Cas12a系统,利用T4 DNA连接酶和phi29 DNA聚合酶快速扩增环状DNA模板,实现了待测靶标的指数增长。在crRNA的引导下,Cas12a可特异性识别目的靶标并激活其反式切割活性,从而检测体系中的ss DNA荧光信号,并通过荧光定量PCR仪读取和分析荧光信号。研究发现,基于CRISPR-Cas12a系统的重金属汞离子荧光检测方法能快速、灵敏地检测汞离子,为重金属污染的快速检测提供新的解决方案。 展开更多
关键词 汞离子检测 CRISPR-Cas12a系统 rca技术 检测新方法
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基于RCA法的多部门协作对提高住院患者抗菌药物治疗前病原学送检效果的评价研究 被引量:1
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作者 潘惠娟 魏薇 《中国医疗管理科学》 2025年第1期114-119,共6页
目的探讨基于RCA法的多部门协作干预对住院患者抗菌药物治疗前病原学送检的影响。方法选取某三级老年病医院2022年1月至2023年12月治疗性使用抗菌药物的住院患者为研究对象,其中2022年1月—12月为对照组,2023年1月—12月为干预组。采取... 目的探讨基于RCA法的多部门协作干预对住院患者抗菌药物治疗前病原学送检的影响。方法选取某三级老年病医院2022年1月至2023年12月治疗性使用抗菌药物的住院患者为研究对象,其中2022年1月—12月为对照组,2023年1月—12月为干预组。采取基于RCA法的多部门协作机制对住院患者抗菌药物治疗前病原学送检情况进行干预,比较干预前后两组患者病原学送检相关监测指标。结果采取多部门协作机制干预后,抗菌药物治疗前病原学送检率、医院感染诊断相关病原学送检率、限制级抗菌药物治疗前病原学送检率分别从52.99%、83.99%、59.61%提高至59.46%、90.15%、64.23%,抗菌药物使用强度从36.42 DDDs下降至29.24 DDDs,差异均有统计学意义(P<0.05)。联合使用重点药物前病原学送检率、特殊使用级抗菌药物治疗前病原学送检率分别从97.06%、95.33%提高到98.91%、95.36%,差异均无统计学意义(P>0.05)。结论基于RCA法的多部门协作干预机制可以有效提高住院患者抗菌药物治疗前病原学送检各项指标。 展开更多
关键词 多部门协作干预 rca 病原学送检 抗菌药物 医院感染管理
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Structural Color Dynamic Graphics Display Based on Microlens Array 被引量:1
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作者 LI Xue-han LIU Ling-zhi +1 位作者 HUANG Min LI Xiu 《印刷与数字媒体技术研究》 北大核心 2025年第2期162-168,共7页
It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be... It is of great scientific significance to construct a 3D dynamic structural color with a special color effect based on the microlens array.However,the problems of imperfect mechanisms and poor color quality need to be solved.A method of 3D structural color turning on periodic metasurfaces fabricated by the microlens array and self-assembly technology was proposed in this study.In the experiment,Polydimethylsiloxane(PDMS)flexible film was used as a substrate,and SiO2 microspheres were scraped into grooves of the PDMS film to form 3D photonic crystal structures.By adjusting the number of blade-coated times and microsphere concentrations,high-saturation structural color micropatterns were obtained.These films were then matched with microlens arrays to produce dynamic graphics with iridescent effects.The results showed that by blade-coated two times and SiO2 microsphere concentrations of 50%are the best conditions.This method demonstrates the potential for being widely applied in the anticounterfeiting printing and ultra-high-resolution display. 展开更多
关键词 Structural color Microlens array Dynamic graphics display Moirémagnification Optical anti-counterfeiting
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Improving DOA estimation of GNSS interference through sparse non-uniform array reconfiguration 被引量:3
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作者 Rongling LANG Hao XU +3 位作者 Fei GAO Zewen TANG Zhipeng WANG Amir HUSSAIN 《Chinese Journal of Aeronautics》 2025年第8期104-118,共15页
Interference significantly impacts the performance of the Global Navigation Satellite Systems(GNSS),highlighting the need for advanced interference localization technology to bolster anti-interference and defense capa... Interference significantly impacts the performance of the Global Navigation Satellite Systems(GNSS),highlighting the need for advanced interference localization technology to bolster anti-interference and defense capabilities.The Uniform Circular Array(UCA)enables concurrent estimation of the Direction of Arrival(DOA)in both azimuth and elevation.Given the paramount importance of stability and real-time performance in interference localization,this work proposes an innovative approach to reduce the complexity and increase the robustness of the DOA estimation.The proposed method reduces computational complexity by selecting a reduced number of array elements to reconstruct a non-uniform sparse array from a UCA.To ensure DOA estimation accuracy,minimizing the Cramér-Rao Bound(CRB)is the objective,and the Spatial Correlation Coefficient(SCC)is incorporated as a constraint to mitigate side-lobe.The optimization model is a quadratic fractional model,which is solved by Semi-Definite Relaxation(SDR).When the array has perturbations,the mathematical expressions for CRB and SCC are re-derived to enhance the robustness of the reconstructed array.Simulation and hardware experiments validate the effectiveness of the proposed method in estimating interference DOA,showing high robustness and reductions in hardware and computational costs associated with DOA estimation. 展开更多
关键词 GNSS interference location Direction of arrival estimation Adaptive reconfigurable array Cramér-Raobound Quadratic fractional programming
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Confronting pressure and humidity limitations in gold nanoparticle sensors array for enhancing electronic nose technology in real-world applications 被引量:1
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作者 Xue Jiang Dan-Yao Qu +11 位作者 Jun-Nan Zhang Viki Kloper Ke-Fan Zhang Guang-Jian Zhang Lei Wang Ming-Hu Pan Quan-Min Guo Li Chen Wei-Tian Huang Jian-Zhi Gao Hossam Haick Wei-Wei Wu 《Rare Metals》 2025年第11期8789-8801,共13页
The field of disease smelling diagnosis has experienced a major breakthrough with the development of the electronic nose(E-nose)that utilizes thiol-capped gold nanoparticles(GNPs).This study focuses on overcoming the ... The field of disease smelling diagnosis has experienced a major breakthrough with the development of the electronic nose(E-nose)that utilizes thiol-capped gold nanoparticles(GNPs).This study focuses on overcoming the challenges of sensors to detect VOCs stably confounding with interferents of humidity and pressure,and clarifying their essential mechanism.An innovative and straightforward method for synthesizing GNPs and modifying their surface has been developed.This unique approach deviates from the conventional Brust method by completely removing any traces of tetraoctylammonium bromide(TOAB),ensuring reproducibility and simplicity of use.This modification enhances the sensor's responsiveness to both polar and non-polar VOCs,as well as strengthen selectivity and anti-interferant capabilities.The mechanism of generating sensing cross-talk from TOAB residue is proposed through rigorous sensing experiments,X-ray photoelectron spectroscopy(XPS)results,and theoretical analysis.Thus,this study enhances the responsiveness of the sensor to both polar and non-polar VOCs,while also strengthening its selectivity and anti-interferent capabilities.This could potentially revolutionize the practical applications of E-nose in smelling diagnosis. 展开更多
关键词 Gold nanoparticles Volatile organic compounds Gas sensor Humidity insensitive Sensors array
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