Objective:To study the antimicrobial,hemolytic activity and immunomodulatory activity of fish epidermal mucus and their chemical constituents from Cynoglossus arel(C.arel) and Alius caelatus(A.caelatus).Mucus plays an...Objective:To study the antimicrobial,hemolytic activity and immunomodulatory activity of fish epidermal mucus and their chemical constituents from Cynoglossus arel(C.arel) and Alius caelatus(A.caelatus).Mucus plays an important role in the prevention of colonization by parasites,bacteria and fungi.Methods:Epidermal mucus was obtained from two marine fishes,lyophilized and the chemical composition of epidermal mucus was analysed by FT-IR analysis.The in vitro antimicrobial activity against human pathogens(fungi,gram positive and gram-negative bacteria) and also the hemolytic activity and immunomodulatory activity were determined.Results:Totally ten human pathogens were tested against the fish mucus.Out of the ten pathogens,five pathogens have proved to be sensitive to the mucus.Maximum zone of inhibition was observed against Vibrio cholera(V.cholera)(9 mm and 2 mm in diameter),followed by Staphylococcus aureus(S.aureus) with a inhibition zone of(6 mm and 3 mm),Streptococcus areus(S.areus)(5 mm and 4 mm),Vibrio parahemolyticus(V.parahemolyticus)(4 mm and 5 mm) respectively.Conclusions:The present investigation has revealed that positive progresses in the fish mucus extracts against human pathogens and hemolytic activity.But further efforts are required for the purification and isolation of the active antimicrobial compounds in order to establish their possible applications.展开更多
The mitochondrial genome(mitogenome) was 16792 bp in length, containing 13 protein coding genes(PCGs), two rRNA genes(12 S rRNA and 16 S rRNA), 22 tRNA genes, and two main non-coding regions. Among these 37 genes, 28 ...The mitochondrial genome(mitogenome) was 16792 bp in length, containing 13 protein coding genes(PCGs), two rRNA genes(12 S rRNA and 16 S rRNA), 22 tRNA genes, and two main non-coding regions. Among these 37 genes, 28 genes were encoded on the heavy strand, while 9 genes were transcribed on the light strand. The non-coding regions of A. dispar included a control region, a light strand replication and another 11 intergenic spacers. The CR of A. dispar contained 8 conserved sequence blocks(CSBs), a termination-associated sequence(TAS) and a pyrimidine tract. Phylogenetic analysis based on 12 PCGs revealed that A. dispar was genetically closest to Arius arius. The families Schilbeidae, Claroteidae, Mochokidae, and Ariidae formed a closely evolved clade. Molecular information from this research introduces mitogenomice data of A. dispar and suggests the phylogenetic relationships among Siluriformes.展开更多
文摘Objective:To study the antimicrobial,hemolytic activity and immunomodulatory activity of fish epidermal mucus and their chemical constituents from Cynoglossus arel(C.arel) and Alius caelatus(A.caelatus).Mucus plays an important role in the prevention of colonization by parasites,bacteria and fungi.Methods:Epidermal mucus was obtained from two marine fishes,lyophilized and the chemical composition of epidermal mucus was analysed by FT-IR analysis.The in vitro antimicrobial activity against human pathogens(fungi,gram positive and gram-negative bacteria) and also the hemolytic activity and immunomodulatory activity were determined.Results:Totally ten human pathogens were tested against the fish mucus.Out of the ten pathogens,five pathogens have proved to be sensitive to the mucus.Maximum zone of inhibition was observed against Vibrio cholera(V.cholera)(9 mm and 2 mm in diameter),followed by Staphylococcus aureus(S.aureus) with a inhibition zone of(6 mm and 3 mm),Streptococcus areus(S.areus)(5 mm and 4 mm),Vibrio parahemolyticus(V.parahemolyticus)(4 mm and 5 mm) respectively.Conclusions:The present investigation has revealed that positive progresses in the fish mucus extracts against human pathogens and hemolytic activity.But further efforts are required for the purification and isolation of the active antimicrobial compounds in order to establish their possible applications.
基金supported by the National Natural Science Foundation of China (Nos.41806127 and 41906111)the Natural Science Foundation of Guangdong Province (No.2018A030313956)。
文摘The mitochondrial genome(mitogenome) was 16792 bp in length, containing 13 protein coding genes(PCGs), two rRNA genes(12 S rRNA and 16 S rRNA), 22 tRNA genes, and two main non-coding regions. Among these 37 genes, 28 genes were encoded on the heavy strand, while 9 genes were transcribed on the light strand. The non-coding regions of A. dispar included a control region, a light strand replication and another 11 intergenic spacers. The CR of A. dispar contained 8 conserved sequence blocks(CSBs), a termination-associated sequence(TAS) and a pyrimidine tract. Phylogenetic analysis based on 12 PCGs revealed that A. dispar was genetically closest to Arius arius. The families Schilbeidae, Claroteidae, Mochokidae, and Ariidae formed a closely evolved clade. Molecular information from this research introduces mitogenomice data of A. dispar and suggests the phylogenetic relationships among Siluriformes.
