In the paper, the in vitro dissolution of borneol in 12 hours from 6 batches of optimized inhalant samples were investigated. As a new dosage form, the in vitro release apparatus of nasal inhalant was invented and a p...In the paper, the in vitro dissolution of borneol in 12 hours from 6 batches of optimized inhalant samples were investigated. As a new dosage form, the in vitro release apparatus of nasal inhalant was invented and a pushing bump was used according to the simulation of the nose expiration and inspiration. Based on the data of r2 in the profile and similar factor f2 from 6 linear release tendencies, a good controlled release and a zero order tendency were observed. It can be suggested that there is a good correlation between the in vitro controlled release and the nose steady self-controllable expiration and inspiration, which will contribute to the trend of insoluble volatile drug controlled release and the effect of quick absorption in nasal pulmonary delivery to cure severe or acute cardiovascular or lung diseases at patients' sleeping, such as angina or breathing obstruction. Also, it was concluded that the prescription composed of insoluble volatile drugs can be prepared to be nasal inhalant from which drugs can be absorbed through nose steady self-controllable inspiration to the lung then into the blood and have a great effectiveness improvement of bioavailability at night timing drug delivery system.展开更多
The Anxin brand burglar-proof alarm padlock is manufactured by the Shanghai New Star Special Equipment Company. It has the combined effect of locking, alarm and guarding against theft.
Objective： To investigate the microR NAs（miR NAs） expression profile of acute myocardial infarction（AMI） rats and the regulating effects of Huoxue Anxin Recipe（活血安心方, HAR） on angiogenesis-related miR NAs a...Objective： To investigate the microR NAs（miR NAs） expression profile of acute myocardial infarction（AMI） rats and the regulating effects of Huoxue Anxin Recipe（活血安心方, HAR） on angiogenesis-related miR NAs and genes. Methods： Forty-five Wistar rats were randomly assigned to 3 groups according to a random number table： sham, AMI, and AMI＋HAR groups（15 in each group）. AMI rats were established by ligation of the left descending coronary artery. HAR was intragastrically administered to rats of the AMI＋HAR group for successive 21 days since modeling, meanwhile the same volume of 0.9% normal saline was administered to rats of the sham and AMI groups. Doppler echocardiography was used for noninvasive cardiac function test. Hematoxylin and eosin staining was used to observe the histopathological change. miR NAs expression profile was detected by quantitative realtime polymerase chain reaction（qR T-PCR）. The mR NA and protein expressions of vascular endothelial growth factor（VEGF）, and a target gene of miR-210 was further detected by qR T-PCR and Western blot, respectively. The microvessels density of myocardium was evaluated by CD31 immunostaining. Results： Compared with the sham group, ejection fraction（EF） and fractional shortening（FS） values were decreased significantly in the AMI group（P〈0.01）, while the infarction area and the interstitial collagen deposition were increased obviously. As for the AMI＋HAR group, EF and FS values were increased significantly（P〈0.05 vs. AMI group）, and the infarction area was reduced and the interstitial collagen deposition were alleviated significantly. Total of 23 miR NAs in the AMI group expressed differently by at least 1.5 folds compared with those in the sham group; 5 miR NAs in the AMI＋HAR group expressed differently by at least 1.5 folds compared with those in the AMI group. Among them, miR-210 was low in the AMI group and high in the AMI＋HAR group. The relative mR NA and protein expressions of VEGF were decreased significantly in the AMI group（P〈0.05 vs. sham group）, and increased significantly in the AMI＋HAR group（P〈0.01 vs. AMI group）. CD31 expression area and optical intensity were decreased significantly in the AMI group（P〈0.05 vs. sham group）, and increased significantly in the AMI＋HAR group（P〈0.01 vs. AMI group）. Conclusions： HAR could reduce the infarction area, alleviate the interstitial fibrosis and improve the cardiac function of AMI rats. Those effects could be related to promoting myocardium angiogenesis of HAR by up-regulating miR-210 and VEGF.展开更多
目的建立柴胡安心胶囊挥发性成分的气相色谱串联质谱(GC-MS)指纹图谱。方法色谱柱为DB-5ms毛细管柱(30 m×0.25 mm,0.25μm),载气为高纯度氦(纯度≥99.999%),流速为1.0 m L/min,进样口温度为250℃,进样量为1μL,程序升温,分流比为20...目的建立柴胡安心胶囊挥发性成分的气相色谱串联质谱(GC-MS)指纹图谱。方法色谱柱为DB-5ms毛细管柱(30 m×0.25 mm,0.25μm),载气为高纯度氦(纯度≥99.999%),流速为1.0 m L/min,进样口温度为250℃,进样量为1μL,程序升温,分流比为20∶1,分流进样;电子轰击离子源,轰击能量为70 e V,离子源温度为230℃,传输线温度为250℃,溶剂延迟时间为4 min,质荷比为55~550。采用"中药色谱指纹图谱超信息特征数字化评价系统3.0"软件,以峰面积最大的色谱峰为参照峰,确定共有峰;采用系统指纹定量法判定样品质量等级;根据美国国家标准与技术局标准质谱数据库及文献检索指认共有峰。结果12批样品的GC-MS图谱有53个共有峰,鉴定出44种化学成分(占总峰面积的99.57%);10批样品质量均在良好以上,2批样品质量为中等;44种化学成分主要包括糠醛(22.50%)、丹皮酚(15.80%)、棕榈酸(12.33%)、桉叶油醇(4.48%)等。结论所建立的GC-MS指纹图谱稳定、可靠,可用于柴胡安心胶囊挥发性成分的研究及整体质量控制,还可为后续药效学研究提供参考。展开更多
文摘In the paper, the in vitro dissolution of borneol in 12 hours from 6 batches of optimized inhalant samples were investigated. As a new dosage form, the in vitro release apparatus of nasal inhalant was invented and a pushing bump was used according to the simulation of the nose expiration and inspiration. Based on the data of r2 in the profile and similar factor f2 from 6 linear release tendencies, a good controlled release and a zero order tendency were observed. It can be suggested that there is a good correlation between the in vitro controlled release and the nose steady self-controllable expiration and inspiration, which will contribute to the trend of insoluble volatile drug controlled release and the effect of quick absorption in nasal pulmonary delivery to cure severe or acute cardiovascular or lung diseases at patients' sleeping, such as angina or breathing obstruction. Also, it was concluded that the prescription composed of insoluble volatile drugs can be prepared to be nasal inhalant from which drugs can be absorbed through nose steady self-controllable inspiration to the lung then into the blood and have a great effectiveness improvement of bioavailability at night timing drug delivery system.
文摘The Anxin brand burglar-proof alarm padlock is manufactured by the Shanghai New Star Special Equipment Company. It has the combined effect of locking, alarm and guarding against theft.
基金Supported by the Major New Drug Development Program from the Ministry of Science and Technology,China(No.2012ZX09102-201-006)
文摘Objective： To investigate the microR NAs（miR NAs） expression profile of acute myocardial infarction（AMI） rats and the regulating effects of Huoxue Anxin Recipe（活血安心方, HAR） on angiogenesis-related miR NAs and genes. Methods： Forty-five Wistar rats were randomly assigned to 3 groups according to a random number table： sham, AMI, and AMI＋HAR groups（15 in each group）. AMI rats were established by ligation of the left descending coronary artery. HAR was intragastrically administered to rats of the AMI＋HAR group for successive 21 days since modeling, meanwhile the same volume of 0.9% normal saline was administered to rats of the sham and AMI groups. Doppler echocardiography was used for noninvasive cardiac function test. Hematoxylin and eosin staining was used to observe the histopathological change. miR NAs expression profile was detected by quantitative realtime polymerase chain reaction（qR T-PCR）. The mR NA and protein expressions of vascular endothelial growth factor（VEGF）, and a target gene of miR-210 was further detected by qR T-PCR and Western blot, respectively. The microvessels density of myocardium was evaluated by CD31 immunostaining. Results： Compared with the sham group, ejection fraction（EF） and fractional shortening（FS） values were decreased significantly in the AMI group（P〈0.01）, while the infarction area and the interstitial collagen deposition were increased obviously. As for the AMI＋HAR group, EF and FS values were increased significantly（P〈0.05 vs. AMI group）, and the infarction area was reduced and the interstitial collagen deposition were alleviated significantly. Total of 23 miR NAs in the AMI group expressed differently by at least 1.5 folds compared with those in the sham group; 5 miR NAs in the AMI＋HAR group expressed differently by at least 1.5 folds compared with those in the AMI group. Among them, miR-210 was low in the AMI group and high in the AMI＋HAR group. The relative mR NA and protein expressions of VEGF were decreased significantly in the AMI group（P〈0.05 vs. sham group）, and increased significantly in the AMI＋HAR group（P〈0.01 vs. AMI group）. CD31 expression area and optical intensity were decreased significantly in the AMI group（P〈0.05 vs. sham group）, and increased significantly in the AMI＋HAR group（P〈0.01 vs. AMI group）. Conclusions： HAR could reduce the infarction area, alleviate the interstitial fibrosis and improve the cardiac function of AMI rats. Those effects could be related to promoting myocardium angiogenesis of HAR by up-regulating miR-210 and VEGF.
文摘目的建立柴胡安心胶囊挥发性成分的气相色谱串联质谱(GC-MS)指纹图谱。方法色谱柱为DB-5ms毛细管柱(30 m×0.25 mm,0.25μm),载气为高纯度氦(纯度≥99.999%),流速为1.0 m L/min,进样口温度为250℃,进样量为1μL,程序升温,分流比为20∶1,分流进样;电子轰击离子源,轰击能量为70 e V,离子源温度为230℃,传输线温度为250℃,溶剂延迟时间为4 min,质荷比为55~550。采用"中药色谱指纹图谱超信息特征数字化评价系统3.0"软件,以峰面积最大的色谱峰为参照峰,确定共有峰;采用系统指纹定量法判定样品质量等级;根据美国国家标准与技术局标准质谱数据库及文献检索指认共有峰。结果12批样品的GC-MS图谱有53个共有峰,鉴定出44种化学成分(占总峰面积的99.57%);10批样品质量均在良好以上,2批样品质量为中等;44种化学成分主要包括糠醛(22.50%)、丹皮酚(15.80%)、棕榈酸(12.33%)、桉叶油醇(4.48%)等。结论所建立的GC-MS指纹图谱稳定、可靠,可用于柴胡安心胶囊挥发性成分的研究及整体质量控制,还可为后续药效学研究提供参考。
