Objective:To perform phytochemical analyses on the leaves of Ocimum basilicum L.(O.basilicum),to elucidate the structure of isolate and then perform the antisickling activity on the crude extract and on the isolate.Me...Objective:To perform phytochemical analyses on the leaves of Ocimum basilicum L.(O.basilicum),to elucidate the structure of isolate and then perform the antisickling activity on the crude extract and on the isolate.Methods:The Emmel test performed on the acidified methanolic extract of this plant was used to evaluate the antisickling activity.The structure characterization of the active compound was performed using chromatographic techniques for the separation and the spectroscopic ones for structure elucidation(1H-NMR,13C-NMR.COSY,HMBC).Resurte:The chemical screening on the crude extract revealed the presence of polyphenols(flavonoids,anthocyanins,leucoanthocyanins,tannins,quinones) alkaloids,saponins,triterpenoids and steroids.The ohtained extract after evaporation yielded 34.50g(11.5%) out of 300g of powdered leaves of O.basilicum.The acidified methanolic extract and butyl stearate showed an interesting antisickling activity.Conchisions:The acidified methanolic extract and butyl stearate from O.basilicum displayed a good antisickling activity.To the best of our knowledge,this is the first time to report the antisickling activity of this compound in this plant.The synmesized compound presented the same spectroscopic characteristics than the natural one and the antisickling activities of its derivatives are understudying.展开更多
<span style="line-height:1.5;">Natural plant products have been used by the population of the south-eastern part of C</span><span lang="EN-US" style="line-height:1.5;">&...<span style="line-height:1.5;">Natural plant products have been used by the population of the south-eastern part of C</span><span lang="EN-US" style="line-height:1.5;">ô</span><span style="line-height:1.5;">te d’Ivoire in the management of sickle cell anemia. </span><span style="line-height:1.5;">This study was aimed at investigating the antisickling activity of the hydro-ethanolic extract </span><span "="" style="line-height:1.5;"><span>of a combination of the leaves of </span><i><span>J</span></i><span>. </span><i><span>secunda</span></i><span>, </span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i></span><span style="line-height:1.5;"> a</span><span "="" style="line-height:1.5;"><span>nd </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span>.</span></span><span style="line-height:1.5;"> These three plants species were used in the Ivorian traditional herbal medicine. Preliminary phytochemistry was carried out using standard methods. As for the sickling reversal test, the Hb SS blood sickling was induced with 2% sodium metabisulfite. After 120 minutes of incubation, the plant extract was added. Every 30 minutes for a period of 120 minutes, a drop of the prepared solution was observed at (40</span><span style="line-height:1.5;">×</span><span "="" style="line-height:1.5;"><span>) magnification and the percentage of reversion calculated. The phytochemical analysis revealed the presence of Alkaloids, Flavonoids, Polyphenols, Catechic Tannins, Sterols and Polyterpenes. The results of the reversal test showed that the percentage of sickling reversal effect of the combination of the plants (75.00 ± 4.33</span><sup><span>b,c</span></sup><span>) was highly superior than the negative control (10.17 ± 0.55</span><sup><span>d</span></sup><span>) but was significantly (p < 0.05) the same as the percentage of the individual plant (</span><i><span>J</span></i><span>. </span><i><span>secunda</span></i><span>: 83.50 ± 2.33</span><sup><span>a</span></sup><span>;</span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i><span>: 78.00 ± 3.67</span><sup><span>b,c</span></sup><span> and </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span>: 77.83 ± 2.89</span><sup><span>b,c</span></sup><span>) and the positive control (80.66 ± 2.22</span><sup><span>a,b</span></sup><span>). From the results, the extracts </span><i><span>J. secunda</span></i><span>, </span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i><span> and </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span> have shown to be therapeutically beneficial to the population. Their use is also justified in the management of sickle cell disease in the south-eastern part of C</span></span><span lang="EN-US" style="line-height:1.5;">ô</span><span style="line-height:1.5;">te d’Ivoire.展开更多
The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum.Biological testing revealed that the plant extracts possess antisickling effects.The combination...The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum.Biological testing revealed that the plant extracts possess antisickling effects.The combination of spectroscopic techniques:1D-NMR,2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O.gratissimum(Silva et al.in Molecules 13:2482–2487,2008;Kedar et al.J Food Drug Anal 20:865–871,2012).This study is the first report of the antisickling activity of ursolic acid isolated from O.gratissimum.The pharmaceutical relevance of findings from this study derives from the possibility of integrating O.gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo.The identification of the active principle could enhance the standardization of antisickling recipe.展开更多
The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democrat...The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democratic Republic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed by TLC and HPLC analysis, showed that rosmarinic acid is the most abundant phenolic acid in these Ocimum species according to the following order O. basilicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. basilicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would be among the main active molecules in these extracts.展开更多
Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat Sickle Cell Disease. These medicinal plants have bee...Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat Sickle Cell Disease. These medicinal plants have been investigated for their inhibitory effect on the induced sickling process of red blood cells using Emmel’s test. Aqueous plant extracts showed good antisickling activity as revealed by the observed normal biconcave form of sickle red blood cells in anoxic conditions. The calculated radius of treated red blood cells by Euphorbia hirta L., Diclipteracolorata C. B. Clarke and Sorghum biocolor (L.) Moench extracts are 3.31 ± 0.55;3.34 ± 0.53;3.17 ± 0.52, respectively. Euphorbia hirta L. is the most active with a normalization rate, more than 70%. The chemical screening performed on these plants showed the presence of anthocyanins which were then extracted. The test carried out with anthocyanin extracts showed that these phenolic compounds have a good antisickling activity and, additionally, possess an anti-hemolytic effect on SS blood. This suggests that the evidenced biological activity of these plants would be due to anthocyanins. The results hence obtained justify the use of these plants in Congolese traditional medicine.展开更多
Sickle cell disease (SCD) is a genetic blood disorder that affects the shape and transportation of red blood cells (RBCs) in blood vessels, leading to various clinical complications. Sickle cell disease is a widesprea...Sickle cell disease (SCD) is a genetic blood disorder that affects the shape and transportation of red blood cells (RBCs) in blood vessels, leading to various clinical complications. Sickle cell disease is a widespread genetic disease in Black Africa. The objective of this study was to evaluate the sickling inhibition activity of Griffonia simplicifolia. Quantitative and qualitative tests were used to determine the major groups of secondary metabolites present in the Griffonia simplicifolia leaves extracts and the modified Emmel test was used to perform the study of sickling inhibition activity. The OECD 423 toxicity study showed that at a single dose of 2000 mg/kg bw, Griffonia simplicifolia leaves extracts are not toxic. All tested substances inhibit erythrocyte falciformation in a dose-dependent manner. The percentages of inhibition were 50.35%, 73% and 94.23% for aqueous extract, hydromethanolic extract and phenylalanine respectively at the concentration of 15 mg/mL. The methanolic extract (70%) had higher activity compared to the aqueous extract. These results suggest that the Griffonia simplicifolia extracts have some potential to be used as alternative antisickling therapy in SCD management.展开更多
基金Supported by the International Foundation for Science(IFS)the Organization for the Prohibition of Chemical Weapons(OPCW)for the Research Grant(IFS Research Grant F/4921-2)
文摘Objective:To perform phytochemical analyses on the leaves of Ocimum basilicum L.(O.basilicum),to elucidate the structure of isolate and then perform the antisickling activity on the crude extract and on the isolate.Methods:The Emmel test performed on the acidified methanolic extract of this plant was used to evaluate the antisickling activity.The structure characterization of the active compound was performed using chromatographic techniques for the separation and the spectroscopic ones for structure elucidation(1H-NMR,13C-NMR.COSY,HMBC).Resurte:The chemical screening on the crude extract revealed the presence of polyphenols(flavonoids,anthocyanins,leucoanthocyanins,tannins,quinones) alkaloids,saponins,triterpenoids and steroids.The ohtained extract after evaporation yielded 34.50g(11.5%) out of 300g of powdered leaves of O.basilicum.The acidified methanolic extract and butyl stearate showed an interesting antisickling activity.Conchisions:The acidified methanolic extract and butyl stearate from O.basilicum displayed a good antisickling activity.To the best of our knowledge,this is the first time to report the antisickling activity of this compound in this plant.The synmesized compound presented the same spectroscopic characteristics than the natural one and the antisickling activities of its derivatives are understudying.
文摘<span style="line-height:1.5;">Natural plant products have been used by the population of the south-eastern part of C</span><span lang="EN-US" style="line-height:1.5;">ô</span><span style="line-height:1.5;">te d’Ivoire in the management of sickle cell anemia. </span><span style="line-height:1.5;">This study was aimed at investigating the antisickling activity of the hydro-ethanolic extract </span><span "="" style="line-height:1.5;"><span>of a combination of the leaves of </span><i><span>J</span></i><span>. </span><i><span>secunda</span></i><span>, </span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i></span><span style="line-height:1.5;"> a</span><span "="" style="line-height:1.5;"><span>nd </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span>.</span></span><span style="line-height:1.5;"> These three plants species were used in the Ivorian traditional herbal medicine. Preliminary phytochemistry was carried out using standard methods. As for the sickling reversal test, the Hb SS blood sickling was induced with 2% sodium metabisulfite. After 120 minutes of incubation, the plant extract was added. Every 30 minutes for a period of 120 minutes, a drop of the prepared solution was observed at (40</span><span style="line-height:1.5;">×</span><span "="" style="line-height:1.5;"><span>) magnification and the percentage of reversion calculated. The phytochemical analysis revealed the presence of Alkaloids, Flavonoids, Polyphenols, Catechic Tannins, Sterols and Polyterpenes. The results of the reversal test showed that the percentage of sickling reversal effect of the combination of the plants (75.00 ± 4.33</span><sup><span>b,c</span></sup><span>) was highly superior than the negative control (10.17 ± 0.55</span><sup><span>d</span></sup><span>) but was significantly (p < 0.05) the same as the percentage of the individual plant (</span><i><span>J</span></i><span>. </span><i><span>secunda</span></i><span>: 83.50 ± 2.33</span><sup><span>a</span></sup><span>;</span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i><span>: 78.00 ± 3.67</span><sup><span>b,c</span></sup><span> and </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span>: 77.83 ± 2.89</span><sup><span>b,c</span></sup><span>) and the positive control (80.66 ± 2.22</span><sup><span>a,b</span></sup><span>). From the results, the extracts </span><i><span>J. secunda</span></i><span>, </span><i><span>J</span></i><span>. </span><i><span>gossypiifolia</span></i><span> and </span><i><span>P</span></i><span>. </span><i><span>nigrescens</span></i><span> have shown to be therapeutically beneficial to the population. Their use is also justified in the management of sickle cell disease in the south-eastern part of C</span></span><span lang="EN-US" style="line-height:1.5;">ô</span><span style="line-height:1.5;">te d’Ivoire.
文摘The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum.Biological testing revealed that the plant extracts possess antisickling effects.The combination of spectroscopic techniques:1D-NMR,2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O.gratissimum(Silva et al.in Molecules 13:2482–2487,2008;Kedar et al.J Food Drug Anal 20:865–871,2012).This study is the first report of the antisickling activity of ursolic acid isolated from O.gratissimum.The pharmaceutical relevance of findings from this study derives from the possibility of integrating O.gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo.The identification of the active principle could enhance the standardization of antisickling recipe.
文摘The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democratic Republic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed by TLC and HPLC analysis, showed that rosmarinic acid is the most abundant phenolic acid in these Ocimum species according to the following order O. basilicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. basilicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would be among the main active molecules in these extracts.
文摘Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat Sickle Cell Disease. These medicinal plants have been investigated for their inhibitory effect on the induced sickling process of red blood cells using Emmel’s test. Aqueous plant extracts showed good antisickling activity as revealed by the observed normal biconcave form of sickle red blood cells in anoxic conditions. The calculated radius of treated red blood cells by Euphorbia hirta L., Diclipteracolorata C. B. Clarke and Sorghum biocolor (L.) Moench extracts are 3.31 ± 0.55;3.34 ± 0.53;3.17 ± 0.52, respectively. Euphorbia hirta L. is the most active with a normalization rate, more than 70%. The chemical screening performed on these plants showed the presence of anthocyanins which were then extracted. The test carried out with anthocyanin extracts showed that these phenolic compounds have a good antisickling activity and, additionally, possess an anti-hemolytic effect on SS blood. This suggests that the evidenced biological activity of these plants would be due to anthocyanins. The results hence obtained justify the use of these plants in Congolese traditional medicine.
文摘Sickle cell disease (SCD) is a genetic blood disorder that affects the shape and transportation of red blood cells (RBCs) in blood vessels, leading to various clinical complications. Sickle cell disease is a widespread genetic disease in Black Africa. The objective of this study was to evaluate the sickling inhibition activity of Griffonia simplicifolia. Quantitative and qualitative tests were used to determine the major groups of secondary metabolites present in the Griffonia simplicifolia leaves extracts and the modified Emmel test was used to perform the study of sickling inhibition activity. The OECD 423 toxicity study showed that at a single dose of 2000 mg/kg bw, Griffonia simplicifolia leaves extracts are not toxic. All tested substances inhibit erythrocyte falciformation in a dose-dependent manner. The percentages of inhibition were 50.35%, 73% and 94.23% for aqueous extract, hydromethanolic extract and phenylalanine respectively at the concentration of 15 mg/mL. The methanolic extract (70%) had higher activity compared to the aqueous extract. These results suggest that the Griffonia simplicifolia extracts have some potential to be used as alternative antisickling therapy in SCD management.
