BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the k...BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the known bioactive components,such as geniposide and crocin,has been the primary focus of the research on G.jasminoides.However,the identification of unknown bioactive components and their metabolomics remains underexplored.Therefore,analysing the metabolic differences between gardenias from different sources is essential to provide a comprehensive theoretical basis for the evaluation of G.jasminoides and germplasm resource identification.AIM To systematically evaluate the morphology,secondary metabolites,typical active ingredients,and antioxidant activity of wild G.jasminoides fruits.METHODS Gardenia fruits were collected from different provenances.Metabolites were identified via ultra-high-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS).The metabolic differences were compared using hierarchical cluster analysis(HCA).The antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays and ferric reducing antioxidant power assays,and its correlation with typical active ingredients was analysed.RESULTS A total of 444 and 240 metabolites were identified using UPLC-MS/MS in positive and negative ion modes,respectively.The HCA results of the flavonoids indicated that the higher content of flavonoids was in the fruits from Lukou.The differential analysis of metabolites in fruits from Shaoyang,Miluo and Lukou showed that the fruit from Miluo had the highest upregulated differential metabolites.CONCLUSION The metabolic characteristics of the Ningxiang and Xiangxi extracts were similar,while those of Lukou,Miluo and Shaoyang extracts differed significantly.展开更多
Marine fungi are potentially important resources for bioactive lead compounds for discovering new drugs for diseases such as Alzheimer's disease.In this paper,the combined bioassay model of acetylcholinesterase(AC...Marine fungi are potentially important resources for bioactive lead compounds for discovering new drugs for diseases such as Alzheimer's disease.In this paper,the combined bioassay model of acetylcholinesterase(AChE)inhibition,1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging,and Artemi ci larval lethality was used to evaluate the activity and toxicity of 35 n narine fun gal strains from seas around China.Their bioactive constitue nfs were revealed by thin layer chromatography(TLC)autography,bioactivity coupled LC-MS/MS and Global Natural Products Social Molecular Networking(GNPS).The results show that the extracts of five strains exhibited higher AChE inhibition ratios than the positive control compound,'tacrine;for which the ratio was 89.8%at 200 pig/ml.Six strains displayed both AChE inhibition(inhibition ratios>20%at 200 pg/ml)and DPPH scavenging activity(scavenging ratios>30%at 200 pg/ml)together with low Artemia larval toxicity(lethal rates<12%).TLC autography showed that the fractioned extracts of four strains contained highly diverse and different bioactive constituents,includi ng strains Talaromyces sp.C2\-\,Aspergillus ter re us C23-3,Trichoderma harzianum DLEN2008005,and Penicillium corylophilum TBG1-17.From the most potent sample F-l 1-1-b(derived from Aspergillus tern us C23-3),five AChE inhibitors and seven antioxida nts were recognized as bioactive molecules by AChE coupled ultrafiltration followed by LC-MS/MS,and LC-MS/MS coupled with DPPH incubation.Furthermore,with the aid of GNPS,the AChE inhibitors were plausibly annotated as territrem analogues including territrems A-C/D,arisugacin A and an unknown compound 4,and the seven antioxidants were assigned as butyrolactone I.aspernolide E,a phenolic derivative and possibly unknown compounds 8-10 and 12.展开更多
On July 23rd, 2009 Carlyle Sinorgchem Shandong Co., Ltd. (Carlyle Sinorgchem) held aground breaking ceremony for its rubber antioxidant expansion project in Cao county,Shandong province.
基金Supported by The Forestry Science and Technology Innovation Funded Project of Hunan Forestry Bureau,No.XLKY202221。
文摘BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the known bioactive components,such as geniposide and crocin,has been the primary focus of the research on G.jasminoides.However,the identification of unknown bioactive components and their metabolomics remains underexplored.Therefore,analysing the metabolic differences between gardenias from different sources is essential to provide a comprehensive theoretical basis for the evaluation of G.jasminoides and germplasm resource identification.AIM To systematically evaluate the morphology,secondary metabolites,typical active ingredients,and antioxidant activity of wild G.jasminoides fruits.METHODS Gardenia fruits were collected from different provenances.Metabolites were identified via ultra-high-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS).The metabolic differences were compared using hierarchical cluster analysis(HCA).The antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays and ferric reducing antioxidant power assays,and its correlation with typical active ingredients was analysed.RESULTS A total of 444 and 240 metabolites were identified using UPLC-MS/MS in positive and negative ion modes,respectively.The HCA results of the flavonoids indicated that the higher content of flavonoids was in the fruits from Lukou.The differential analysis of metabolites in fruits from Shaoyang,Miluo and Lukou showed that the fruit from Miluo had the highest upregulated differential metabolites.CONCLUSION The metabolic characteristics of the Ningxiang and Xiangxi extracts were similar,while those of Lukou,Miluo and Shaoyang extracts differed significantly.
基金This work was supported by the Yangfan Talent Project of Guangdong Province under Grant 201433009the Natural Science Foundation of Guangdong Province under Grant 2018A030307046+7 种基金the Basic Research Project of Shenzhen Science and Technology Innovation Commission under Grant JCYJ20190813105005619the Shenzhen Dapeng New District Industrial Development Fund under Grants KY20180203&PT201901-05the Shenzhen Dapeng New District Scientific and Technological Research and Development Fund under Grant KJYF202001-07the Natural Science Foundation of China under Grant 21807015the Program for Scientific Research Start-Up Funds of Guangdong Ocean University under Grant E15155the Applied S&T Research and Development Major Project of Guangdong Province under Grant 2016B020235001the Scientific Fund of the Zhanjiang Municipal Oceanic and Fishery Bureau under Grant A18018the Project of Enhancing School with Innovation of Guangdong Ocean University(230420022).
文摘Marine fungi are potentially important resources for bioactive lead compounds for discovering new drugs for diseases such as Alzheimer's disease.In this paper,the combined bioassay model of acetylcholinesterase(AChE)inhibition,1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging,and Artemi ci larval lethality was used to evaluate the activity and toxicity of 35 n narine fun gal strains from seas around China.Their bioactive constitue nfs were revealed by thin layer chromatography(TLC)autography,bioactivity coupled LC-MS/MS and Global Natural Products Social Molecular Networking(GNPS).The results show that the extracts of five strains exhibited higher AChE inhibition ratios than the positive control compound,'tacrine;for which the ratio was 89.8%at 200 pig/ml.Six strains displayed both AChE inhibition(inhibition ratios>20%at 200 pg/ml)and DPPH scavenging activity(scavenging ratios>30%at 200 pg/ml)together with low Artemia larval toxicity(lethal rates<12%).TLC autography showed that the fractioned extracts of four strains contained highly diverse and different bioactive constituents,includi ng strains Talaromyces sp.C2\-\,Aspergillus ter re us C23-3,Trichoderma harzianum DLEN2008005,and Penicillium corylophilum TBG1-17.From the most potent sample F-l 1-1-b(derived from Aspergillus tern us C23-3),five AChE inhibitors and seven antioxida nts were recognized as bioactive molecules by AChE coupled ultrafiltration followed by LC-MS/MS,and LC-MS/MS coupled with DPPH incubation.Furthermore,with the aid of GNPS,the AChE inhibitors were plausibly annotated as territrem analogues including territrems A-C/D,arisugacin A and an unknown compound 4,and the seven antioxidants were assigned as butyrolactone I.aspernolide E,a phenolic derivative and possibly unknown compounds 8-10 and 12.
文摘On July 23rd, 2009 Carlyle Sinorgchem Shandong Co., Ltd. (Carlyle Sinorgchem) held aground breaking ceremony for its rubber antioxidant expansion project in Cao county,Shandong province.
