OBJECTIVE: To assess the phytochemical contents and antioxidant activities of crude extracts from selected Tulbaghia species.METHODS: Standard methods were used for preliminary phytochemical analysis. The total phenol...OBJECTIVE: To assess the phytochemical contents and antioxidant activities of crude extracts from selected Tulbaghia species.METHODS: Standard methods were used for preliminary phytochemical analysis. The total phenolic acid contents of the plant extracts were determined using the Folin-Ciocalteu method, and the total flavonoid contents were determined using the aluminum chloride colorimetric method. 1,1-Diphenyl-2-picrylhydrazyl and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays were used to evaluate the antioxidant activities.RESULTS: Phytochemical screening showed flavonoids, glycosides, tannins, terpenoids, saponins,and steroids were present in the Tulbaghia species.The total phenolic acid and flavonoid contents varied in the different plant extracts, ranging from4.50 to 11.10 mg of gallic acid equivalents per gram of fresh material and 3.04 to 9.65 mg of quercetin equivalents per gram, respectively. The IC50 values determined for Tulbaghia alliacea and Tulbaghia violacea based on 1,1-diphenyl-2-picrylhydrazyl(0.06 and 0.08 mg/m L, respectively) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(0.06 and 0.03 mg/m L, respectively) were low and showed they had potential antioxidant activities.CONCLUSION: Our results suggest that individual compounds from Tulbaghia species should be isolated for analysis of their antioxidant activity because some compounds may work best when pure.展开更多
OBJECTIVE: To investigate the effects of Zibushenjing Fang (formula for tonifying the kidney essence) on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency. METHODS: Fifty ma...OBJECTIVE: To investigate the effects of Zibushenjing Fang (formula for tonifying the kidney essence) on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency. METHODS: Fifty male Kunming mice were randomly divided into five groups: normal group, model group, Jinkuishenqi Wan group, Zibushenjing Fang high dose group, and Zibushenjing Fang low dose group, with 10 mice in each group. The model of kidney essence insufficiency syndrome was established in all the mice except the normal group by using a cat to threaten the mice and by swimming until exhaustion daily which lasted about 21 days. Mice in the model group were administered 20 mL/ kg -1 · d -1 of normal saline intragastrically. The Jinkuishenqi Wan group was given 2.7 g/kg-1 · d-1 ofa solution of Jinkuishenqi Wan. The Zibushenjing Fang high dose group was given 20 g/kg -1 · d -1 and the Zibushenjing Fang low dose group was given 10 g/kg -1 · d -1 of a solution of Zibushenjing Fang.The general condition of all the groups was observed, including the quantity of food and water intake, swimming time, length of femur, and weight of the femur and musculus quadriceps femoris. The total superoxide dismutase (T-SOD), glutathione peroxi- dase (GSH-Px), and methane dicarboxylic aldehyde (MDA) activities in the brain tissues were detected. RESULTS: Zibushenjing Fang could improve the manifestation of kidney essence insufficiency, increase the quantity of food and water intake, swimming time, femur length, and femur and musculus quadriceps femoris weight. It could also regulate the activities of T-SOD, GSH-Px, and MDA in brain tissue. CONCLUSION: Zibushenjing Fang may play an important role in treating kidney essence insufficiency syndrome by promoting body development and improving brain tissue antioxidation.展开更多
Several studies report an increase in both male and female factors in infertility worldwide. In recent years there has been a tremendous increase in couples seeking assisted reproductive technology(ART) procedures in ...Several studies report an increase in both male and female factors in infertility worldwide. In recent years there has been a tremendous increase in couples seeking assisted reproductive technology(ART) procedures in order to have children. However, the success rates of these procedures still remain very low. One of the major contributing factors to the low success rate in ART has been the damage caused by free radicals to the gametes and the developing embryo. The manipulation of gametes and embryos in an in vitro environment when performing assisted reproductive techniques carries the risk of exposure of these cells to supraphysiological levels of free radicals; namely, reactive oxygen species(ROS) and reactive nitrogen species. Oxidative stress can originate from the early steps of ART involving the oocyte, sperm and embryo, as well as in the endometrial environment later on following embryo transfer. The common sources of free radicals in an in vitro fertilization setting include the developing embryo, spermatozoa and leukocytes, semen centrifugation, oxygen partial pressure, light, culture media and cryopreservation/thawing. These free radicals are measured using different techniques, such as the cytochrome C reduction method and chemiluminescence-based techniques. Different efforts are being employed to minimize theexcess generation of free radicals in the ART setting, with the aim of improving the success rate, and antioxidant supplementation has emerged as one of the viable routes. Moreover, it is very important to inform ART personnel about the sources of ROS in the laboratory so that they can stop the use of procedures that are deleterious and start to use safer procedures.展开更多
Reactive oxygen species(ROS) take part in diverse biological processes like cell growth,programmed cell death,cell senescence,and maintenance of the transformed state through regulation of signal transduction. Cancer ...Reactive oxygen species(ROS) take part in diverse biological processes like cell growth,programmed cell death,cell senescence,and maintenance of the transformed state through regulation of signal transduction. Cancer cells adapt to new higher ROS circumstance. Sometimes,ROS induce cancer cell proliferation. Meanwhile,elevated ROS render cancer cells vulnerable to oxidative stress-induced cell death. However,this prominent character of cancer cells allows acquiring a resistance to oxidative stress conditions relative to normal cells. Activated signaling pathways that increase the level of intracellular ROS in cancer cells not only render up-regulation of several genes involved in cellular proliferation and evasion of apoptosis but also cause cancer cells and cancer stem cells to develop a high metabolic rate. In over the past several decades,many studies have indicated that ROS play a critical role as the secondary messenger of tumorigenesis and metastasis in cancer from both in vitro and in vivo. Here we summarize the role of ROS and anti-oxidants in contributing to or preventing cancer. In addition,we review the activated signaling pathways that make cancer cells susceptible to death.展开更多
This study aimed at investigating the impact of adding microbial transglutaminase (MTGase) after rennet addition on some properties of fresh soft cheese made from camel milk. MTGase was added to milk at concentration ...This study aimed at investigating the impact of adding microbial transglutaminase (MTGase) after rennet addition on some properties of fresh soft cheese made from camel milk. MTGase was added to milk at concentration of 80, 100 and 120 U/L after 20 and 30 min of renneting. The chemical composition, yield, hardness, antioxidant activity and sensory properties of cheese were estimated. Enzymatic protein crosslinking was analyzed by SDS-PAGE. Results revealed that MTGase-treated cheeses were higher in moisture and lower in protein content compared to control. In addition, the concentration of MTGase and time of addition significantly (P 0.05) impacted these parameters. Among treated cheeses, samples with 80 U of MTGase and addition time of 20 min were the highest in total solids and protein content. Adding MTGase significantly (P 0.05) increased the cheese yield, however, increased MTGase concentration at any time of addition did not improve it. The electrophoretic patterns of MTGase-cheese proteins showed a reduction in the intensity of caseins bands and the appearance of new protein fractions with high molecular weights. However, the changes in the intensity of the whey proteins bands were not sufficiently clear as caseins. The cheese hardness was significantly (P 0.05) affected by adding MTGase. Cheese containing 80 U of MTGase had the highest hardness value compared to control and other treated samples. The antioxidant activity of cheese was negatively influenced by adding the enzyme. The use of MTGase enhanced the mouthfeel, texture and overall acceptability of cheese. However, the effect of MTGase concentration and addition time was not significant (P > 0.05) on the sensory attributes. In conclusion, adding MTGase to milk at concentration of 80 U after 20 min of renneting is recommended to improve the yield, textural and some sensory properties of fresh soft cheese made from camel milk.展开更多
Camellia taliensis belonging to Camellia sect.Thea(Theaceae)is distributed from the western and south-western areas of Yunnan Province,China to the north of Myanmar.Known as the"wild"tea plant,it has been co...Camellia taliensis belonging to Camellia sect.Thea(Theaceae)is distributed from the western and south-western areas of Yunnan Province,China to the north of Myanmar.Known as the"wild"tea plant,it has been com-monly used for making tea by the local people of its growing area.It is the first investigation of the volatile constituents of the fresh tender leaves of C.taliensis and green teas produced from its tender and older leaves.The volatile constituents were obtained by hydrodistillation and analyzed by GC and GC-MS.Ninety-one compounds were identified.The results showed that the main compositions of volatile oil of the fresh tender leaves were hexadecanoic acid(30.52%),linoleic acid(19.82%),phytol(8.75%),and geraniol(2.54%),while monoterpenoids(58.51%)composing of linalool(28.43%),hotrienol(1.13%),a-terpineol(11.68%),nerol(4.92%)and geraniol(12.34%)were the major volatile components of its green tea product.From the fresh leaves to the green tea products,28 aro-ma components were formed.Among then,the content of(Z,Z,Z)-9,12,15-octadecatrien-l-ol(peak 77)was up to 1.21%(from tender leaves)and 11.2%(from older leaves),respectively.The DPPH and ABTS radical scavenging assays demonstrated a moderate activity of essential oil from the three essential oils of C.taliensis.展开更多
基金Supported by Vaal University of Technology,Private Bag X021,Vanderbijlpark 1900,South Africa
文摘OBJECTIVE: To assess the phytochemical contents and antioxidant activities of crude extracts from selected Tulbaghia species.METHODS: Standard methods were used for preliminary phytochemical analysis. The total phenolic acid contents of the plant extracts were determined using the Folin-Ciocalteu method, and the total flavonoid contents were determined using the aluminum chloride colorimetric method. 1,1-Diphenyl-2-picrylhydrazyl and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays were used to evaluate the antioxidant activities.RESULTS: Phytochemical screening showed flavonoids, glycosides, tannins, terpenoids, saponins,and steroids were present in the Tulbaghia species.The total phenolic acid and flavonoid contents varied in the different plant extracts, ranging from4.50 to 11.10 mg of gallic acid equivalents per gram of fresh material and 3.04 to 9.65 mg of quercetin equivalents per gram, respectively. The IC50 values determined for Tulbaghia alliacea and Tulbaghia violacea based on 1,1-diphenyl-2-picrylhydrazyl(0.06 and 0.08 mg/m L, respectively) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(0.06 and 0.03 mg/m L, respectively) were low and showed they had potential antioxidant activities.CONCLUSION: Our results suggest that individual compounds from Tulbaghia species should be isolated for analysis of their antioxidant activity because some compounds may work best when pure.
文摘OBJECTIVE: To investigate the effects of Zibushenjing Fang (formula for tonifying the kidney essence) on skeletal development and brain tissue antioxidation in mice with kidney essence insufficiency. METHODS: Fifty male Kunming mice were randomly divided into five groups: normal group, model group, Jinkuishenqi Wan group, Zibushenjing Fang high dose group, and Zibushenjing Fang low dose group, with 10 mice in each group. The model of kidney essence insufficiency syndrome was established in all the mice except the normal group by using a cat to threaten the mice and by swimming until exhaustion daily which lasted about 21 days. Mice in the model group were administered 20 mL/ kg -1 · d -1 of normal saline intragastrically. The Jinkuishenqi Wan group was given 2.7 g/kg-1 · d-1 ofa solution of Jinkuishenqi Wan. The Zibushenjing Fang high dose group was given 20 g/kg -1 · d -1 and the Zibushenjing Fang low dose group was given 10 g/kg -1 · d -1 of a solution of Zibushenjing Fang.The general condition of all the groups was observed, including the quantity of food and water intake, swimming time, length of femur, and weight of the femur and musculus quadriceps femoris. The total superoxide dismutase (T-SOD), glutathione peroxi- dase (GSH-Px), and methane dicarboxylic aldehyde (MDA) activities in the brain tissues were detected. RESULTS: Zibushenjing Fang could improve the manifestation of kidney essence insufficiency, increase the quantity of food and water intake, swimming time, femur length, and femur and musculus quadriceps femoris weight. It could also regulate the activities of T-SOD, GSH-Px, and MDA in brain tissue. CONCLUSION: Zibushenjing Fang may play an important role in treating kidney essence insufficiency syndrome by promoting body development and improving brain tissue antioxidation.
文摘Several studies report an increase in both male and female factors in infertility worldwide. In recent years there has been a tremendous increase in couples seeking assisted reproductive technology(ART) procedures in order to have children. However, the success rates of these procedures still remain very low. One of the major contributing factors to the low success rate in ART has been the damage caused by free radicals to the gametes and the developing embryo. The manipulation of gametes and embryos in an in vitro environment when performing assisted reproductive techniques carries the risk of exposure of these cells to supraphysiological levels of free radicals; namely, reactive oxygen species(ROS) and reactive nitrogen species. Oxidative stress can originate from the early steps of ART involving the oocyte, sperm and embryo, as well as in the endometrial environment later on following embryo transfer. The common sources of free radicals in an in vitro fertilization setting include the developing embryo, spermatozoa and leukocytes, semen centrifugation, oxygen partial pressure, light, culture media and cryopreservation/thawing. These free radicals are measured using different techniques, such as the cytochrome C reduction method and chemiluminescence-based techniques. Different efforts are being employed to minimize theexcess generation of free radicals in the ART setting, with the aim of improving the success rate, and antioxidant supplementation has emerged as one of the viable routes. Moreover, it is very important to inform ART personnel about the sources of ROS in the laboratory so that they can stop the use of procedures that are deleterious and start to use safer procedures.
文摘Reactive oxygen species(ROS) take part in diverse biological processes like cell growth,programmed cell death,cell senescence,and maintenance of the transformed state through regulation of signal transduction. Cancer cells adapt to new higher ROS circumstance. Sometimes,ROS induce cancer cell proliferation. Meanwhile,elevated ROS render cancer cells vulnerable to oxidative stress-induced cell death. However,this prominent character of cancer cells allows acquiring a resistance to oxidative stress conditions relative to normal cells. Activated signaling pathways that increase the level of intracellular ROS in cancer cells not only render up-regulation of several genes involved in cellular proliferation and evasion of apoptosis but also cause cancer cells and cancer stem cells to develop a high metabolic rate. In over the past several decades,many studies have indicated that ROS play a critical role as the secondary messenger of tumorigenesis and metastasis in cancer from both in vitro and in vivo. Here we summarize the role of ROS and anti-oxidants in contributing to or preventing cancer. In addition,we review the activated signaling pathways that make cancer cells susceptible to death.
文摘This study aimed at investigating the impact of adding microbial transglutaminase (MTGase) after rennet addition on some properties of fresh soft cheese made from camel milk. MTGase was added to milk at concentration of 80, 100 and 120 U/L after 20 and 30 min of renneting. The chemical composition, yield, hardness, antioxidant activity and sensory properties of cheese were estimated. Enzymatic protein crosslinking was analyzed by SDS-PAGE. Results revealed that MTGase-treated cheeses were higher in moisture and lower in protein content compared to control. In addition, the concentration of MTGase and time of addition significantly (P 0.05) impacted these parameters. Among treated cheeses, samples with 80 U of MTGase and addition time of 20 min were the highest in total solids and protein content. Adding MTGase significantly (P 0.05) increased the cheese yield, however, increased MTGase concentration at any time of addition did not improve it. The electrophoretic patterns of MTGase-cheese proteins showed a reduction in the intensity of caseins bands and the appearance of new protein fractions with high molecular weights. However, the changes in the intensity of the whey proteins bands were not sufficiently clear as caseins. The cheese hardness was significantly (P 0.05) affected by adding MTGase. Cheese containing 80 U of MTGase had the highest hardness value compared to control and other treated samples. The antioxidant activity of cheese was negatively influenced by adding the enzyme. The use of MTGase enhanced the mouthfeel, texture and overall acceptability of cheese. However, the effect of MTGase concentration and addition time was not significant (P > 0.05) on the sensory attributes. In conclusion, adding MTGase to milk at concentration of 80 U after 20 min of renneting is recommended to improve the yield, textural and some sensory properties of fresh soft cheese made from camel milk.
文摘Camellia taliensis belonging to Camellia sect.Thea(Theaceae)is distributed from the western and south-western areas of Yunnan Province,China to the north of Myanmar.Known as the"wild"tea plant,it has been com-monly used for making tea by the local people of its growing area.It is the first investigation of the volatile constituents of the fresh tender leaves of C.taliensis and green teas produced from its tender and older leaves.The volatile constituents were obtained by hydrodistillation and analyzed by GC and GC-MS.Ninety-one compounds were identified.The results showed that the main compositions of volatile oil of the fresh tender leaves were hexadecanoic acid(30.52%),linoleic acid(19.82%),phytol(8.75%),and geraniol(2.54%),while monoterpenoids(58.51%)composing of linalool(28.43%),hotrienol(1.13%),a-terpineol(11.68%),nerol(4.92%)and geraniol(12.34%)were the major volatile components of its green tea product.From the fresh leaves to the green tea products,28 aro-ma components were formed.Among then,the content of(Z,Z,Z)-9,12,15-octadecatrien-l-ol(peak 77)was up to 1.21%(from tender leaves)and 11.2%(from older leaves),respectively.The DPPH and ABTS radical scavenging assays demonstrated a moderate activity of essential oil from the three essential oils of C.taliensis.
