Objectives: Garcinia kola seeds (EAgk) (Clusiaceae) contain coagulant components use to pretreat aquaculture wastewater in Nigeria. This work was undertaken to assess the effects of EAgk on blood coagulation because o...Objectives: Garcinia kola seeds (EAgk) (Clusiaceae) contain coagulant components use to pretreat aquaculture wastewater in Nigeria. This work was undertaken to assess the effects of EAgk on blood coagulation because of its contents. Methods: Five groups of four rats of both sexes (200 ± 5 g) received orally distilled water, phytomenadione (15 mg/kg b.w.) and garcinia kola seeds extract (250, 500 and 1000 mg/kg b.w.) for four days and after this period, bleeding time was measured by tail hemorrhage model. Activated partial thromboplastin time (aPTT) and platelet count were determined by coagulometer and hematological analyzer respectively. Blood red cells protection of EAgk was measured by the methods of 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced hemolysis and inhibition of 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical and hydrogen peroxide. Results: EAgk at 1000 mg/kg b.w. induced a significant decrease in bleeding time from 420 ± 27.3 s to 157.5 ± 18.9 s. The platelet count of group of rat treated with EAgk (1000 mg/kg b.w.) increased significantly (p 0.05, n = 4). EAgk inhibited hemolysis, DPPH radical and hydrogen peroxide with IC50 values of 1.02 ± 0.2;3.2 × 10?2 ± 0.12 and 1.01 ± 0.5 mg/mg respectively. Conclusions: EAgk exhibits hemostatic effects, which could justify the presence of coagulant components in the extract. Erythrocytes’ protective activity of Garcinia kola seeds may be due to free radicals and hydrogen peroxide inhibition.展开更多
Objective:To examine the antioxidant and antihemolytic activities of the aqueous extract,total polyphenols and total flavonoids of Thymus satureioides(T.satureioides).Methods:This plant was collected from Tafilalet Re...Objective:To examine the antioxidant and antihemolytic activities of the aqueous extract,total polyphenols and total flavonoids of Thymus satureioides(T.satureioides).Methods:This plant was collected from Tafilalet Region of Morocco.The aqueous extract was obtained by cold maceration,and the components were obtained by Soxhlel extraction using solvents of varying polarity.The identification and quantification of phenol(caffeic and rosmarinic acids) and llavones(luteolin 7-glycoside and hesperetin) were carried out by high performance liquid chromatography analysis.Results:Total polyphenol and flavonoids contents in the aqueous extract of T.satureioides were(456.73±6.94) mg caffeic acid equivalent/g of dry plant and(172.79±2.12) mg rutin equivalent/g of dry plant,respectively.Different extracts showed good antioxidant activity.IC_(50)for 1.1-diphenyl-2-picrylhydrazil radical scavenging activity was(0.480±0.010),(0.418±0.005),(43.S91 ±2.467) and(0.510±0.010) mg/niL for the aqueous extract,total polyphenol,flavonoids and trolox,respectively.Also,the extracts showed ferric reducing antioxidant power and the values were(50.79±2.02),(117.51±6.46).(7.03±0.29) and(44.33±7.55) mmol trolox/g for the aqueous extract,total polyphenol,flavonoids and trolox.respectively.Serum levels of malondialdehyde was significantly decreased in comparison with the oxidized control(P<0.00l).They showed good activity against 2.2.-azobis 2-amidinopropane dihydrochloridc induced hemolysis in erythrocytes of rabbit blood.In addition,they ameliorate the half time of hemolysis.Conclusions:Our results provide evidence that aqueous extract,total polyphenols and total flavonoids of T.satureioides exhibit marked antioxidant and antihemolytic activities,thus confirming and justifying the popular uses of this plant to relieve some pains.展开更多
Background:This study investigated the phenolic profile,antioxidant capacity,antibacterial effect,and antihemolytic activity of nettle leaves from two understudied Algerian species,Urtica pilulifera and Urtica urens.M...Background:This study investigated the phenolic profile,antioxidant capacity,antibacterial effect,and antihemolytic activity of nettle leaves from two understudied Algerian species,Urtica pilulifera and Urtica urens.Methods:Urtica pilulifera and Urtica urens leaves extracts were prepared by maceration using methanol and distilled water respectively.Their phytochemical analysis(total phenolic content,flavonoids,hydrolysable and condensed tannins)was determined.The chemical profle of these extracts was performed using ultra-high-performance liquid chromatography diode array detection tandem mass spectrometry.Antioxidant activity(using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging method),antibacterial activity(using disk diffusion method),and antihemolytic activity were carried out.Results:Results reveal high levels of flavonoids,tannins,alkaloids,and terpenoids.Several classes of compounds were identified in the extracts.Phenolic acid and other acids and their derivatives and flavonoids were detected in the extracts,with 4-O-caffeoyl-quinic acid and 5-O-caffeoyl-quinic acid being the main constituents in both extracts.Caffeic acid was also the main constituent present only in U.urens extract.For antioxidant activity of the methanolic extract of U.pilulifera and the aqueous extract of U.urens,the percentage inhibition value(82.76%and 59.06%)and the half-maximum inhibitory concentration(IC_(50))value(302 and 423μg/mL),respectively,were obtained.Antibacterial activity of nettle extracts(10 mg/mL)demonstrated the sensitivity of pathogenic strain susceptibility.The strongest antibacterial effect on tested strains was found in the aqueous extract of U.urens against Staphylococcus aureus(21±0.41 mm)compared to the methanolic extract of U.pilulifera(16±0.40 mm).The antihemolytic activity in the methanolic and aqueous extracts was 76.26%and 60.67%,respectively.The methanolic extract exhibited exceptional antihemolytic effect,with an IC_(50)value of 327μg/mL,whereas the aqueous extract had an IC_(50)value of 412μg/mL.Conclusions:The study confirms the presence of bioactive substances in the nettle species,including flavonoids and tannins,which possess antioxidant,antibacterial,and antihemolytic properties,and can be processed into food and pharmaceutical products.展开更多
文摘Objectives: Garcinia kola seeds (EAgk) (Clusiaceae) contain coagulant components use to pretreat aquaculture wastewater in Nigeria. This work was undertaken to assess the effects of EAgk on blood coagulation because of its contents. Methods: Five groups of four rats of both sexes (200 ± 5 g) received orally distilled water, phytomenadione (15 mg/kg b.w.) and garcinia kola seeds extract (250, 500 and 1000 mg/kg b.w.) for four days and after this period, bleeding time was measured by tail hemorrhage model. Activated partial thromboplastin time (aPTT) and platelet count were determined by coagulometer and hematological analyzer respectively. Blood red cells protection of EAgk was measured by the methods of 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced hemolysis and inhibition of 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical and hydrogen peroxide. Results: EAgk at 1000 mg/kg b.w. induced a significant decrease in bleeding time from 420 ± 27.3 s to 157.5 ± 18.9 s. The platelet count of group of rat treated with EAgk (1000 mg/kg b.w.) increased significantly (p 0.05, n = 4). EAgk inhibited hemolysis, DPPH radical and hydrogen peroxide with IC50 values of 1.02 ± 0.2;3.2 × 10?2 ± 0.12 and 1.01 ± 0.5 mg/mg respectively. Conclusions: EAgk exhibits hemostatic effects, which could justify the presence of coagulant components in the extract. Erythrocytes’ protective activity of Garcinia kola seeds may be due to free radicals and hydrogen peroxide inhibition.
文摘Objective:To examine the antioxidant and antihemolytic activities of the aqueous extract,total polyphenols and total flavonoids of Thymus satureioides(T.satureioides).Methods:This plant was collected from Tafilalet Region of Morocco.The aqueous extract was obtained by cold maceration,and the components were obtained by Soxhlel extraction using solvents of varying polarity.The identification and quantification of phenol(caffeic and rosmarinic acids) and llavones(luteolin 7-glycoside and hesperetin) were carried out by high performance liquid chromatography analysis.Results:Total polyphenol and flavonoids contents in the aqueous extract of T.satureioides were(456.73±6.94) mg caffeic acid equivalent/g of dry plant and(172.79±2.12) mg rutin equivalent/g of dry plant,respectively.Different extracts showed good antioxidant activity.IC_(50)for 1.1-diphenyl-2-picrylhydrazil radical scavenging activity was(0.480±0.010),(0.418±0.005),(43.S91 ±2.467) and(0.510±0.010) mg/niL for the aqueous extract,total polyphenol,flavonoids and trolox,respectively.Also,the extracts showed ferric reducing antioxidant power and the values were(50.79±2.02),(117.51±6.46).(7.03±0.29) and(44.33±7.55) mmol trolox/g for the aqueous extract,total polyphenol,flavonoids and trolox.respectively.Serum levels of malondialdehyde was significantly decreased in comparison with the oxidized control(P<0.00l).They showed good activity against 2.2.-azobis 2-amidinopropane dihydrochloridc induced hemolysis in erythrocytes of rabbit blood.In addition,they ameliorate the half time of hemolysis.Conclusions:Our results provide evidence that aqueous extract,total polyphenols and total flavonoids of T.satureioides exhibit marked antioxidant and antihemolytic activities,thus confirming and justifying the popular uses of this plant to relieve some pains.
基金Ministry of Science,Technological Development and Innovations of the Republic of Serbia,Grant/Award Number:451-03-137/2025-03/200133。
文摘Background:This study investigated the phenolic profile,antioxidant capacity,antibacterial effect,and antihemolytic activity of nettle leaves from two understudied Algerian species,Urtica pilulifera and Urtica urens.Methods:Urtica pilulifera and Urtica urens leaves extracts were prepared by maceration using methanol and distilled water respectively.Their phytochemical analysis(total phenolic content,flavonoids,hydrolysable and condensed tannins)was determined.The chemical profle of these extracts was performed using ultra-high-performance liquid chromatography diode array detection tandem mass spectrometry.Antioxidant activity(using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging method),antibacterial activity(using disk diffusion method),and antihemolytic activity were carried out.Results:Results reveal high levels of flavonoids,tannins,alkaloids,and terpenoids.Several classes of compounds were identified in the extracts.Phenolic acid and other acids and their derivatives and flavonoids were detected in the extracts,with 4-O-caffeoyl-quinic acid and 5-O-caffeoyl-quinic acid being the main constituents in both extracts.Caffeic acid was also the main constituent present only in U.urens extract.For antioxidant activity of the methanolic extract of U.pilulifera and the aqueous extract of U.urens,the percentage inhibition value(82.76%and 59.06%)and the half-maximum inhibitory concentration(IC_(50))value(302 and 423μg/mL),respectively,were obtained.Antibacterial activity of nettle extracts(10 mg/mL)demonstrated the sensitivity of pathogenic strain susceptibility.The strongest antibacterial effect on tested strains was found in the aqueous extract of U.urens against Staphylococcus aureus(21±0.41 mm)compared to the methanolic extract of U.pilulifera(16±0.40 mm).The antihemolytic activity in the methanolic and aqueous extracts was 76.26%and 60.67%,respectively.The methanolic extract exhibited exceptional antihemolytic effect,with an IC_(50)value of 327μg/mL,whereas the aqueous extract had an IC_(50)value of 412μg/mL.Conclusions:The study confirms the presence of bioactive substances in the nettle species,including flavonoids and tannins,which possess antioxidant,antibacterial,and antihemolytic properties,and can be processed into food and pharmaceutical products.
