The orientation of the biological molecule immobi-lized on a solid surface has been critical in devel-opment of various applications. In this study, ori-entation of antibody was retained by protecting the antigen-bind...The orientation of the biological molecule immobi-lized on a solid surface has been critical in devel-opment of various applications. In this study, ori-entation of antibody was retained by protecting the antigen-binding site of the antibody prior to immo-bilization to -functionalized mixed self-assembled monolayer (SAM) of 12-mercaptododecanoic acid and 1-heptanethiol. More importantly, the number of immobilization bonds formed between each an-tigen-binding site protected antibody molecule and the solid surface was controlled by optimizing the mole fraction of the activated carboxyl group of the linker molecules in the mixed SAM. The amount of antibody used in this study was approximately equivalent to the amount for one monolayer surface coverage. The resulting activity of protected immo-bilized antibody was about 10 fold higher than that of random immobilized展开更多
With Mosuo's sacred natural sites of mountainous forest in Yongning-Lugu Lake area as the research subject,by using the method of ecology,the vegetation type in this area was initially identified.The control sampl...With Mosuo's sacred natural sites of mountainous forest in Yongning-Lugu Lake area as the research subject,by using the method of ecology,the vegetation type in this area was initially identified.The control sample plot of the same vegetation type at similar altitude and in similar soil condition would be selected and compared with the sample plot in sacred natural sites at length,so as to quantitatively analyze the protection of biological diversity in the perspective of ecological system.It showed that under the circumstance of the damage of ecological environment,complete natural vertical pattern was preserved thanks to sacred natural sites of mountainous forest established by reason of traditional religion and culture.The results of research and quantitative analysis showed that compared with the control sample plot of the same vegetation type,sacred natural sites were high in biological diversity and the species composition of plant community in sacred natural sites was closer to that of primitive forest.The result of quantitative analysis also indicated that sacred natural sites played a remarkable role in protecting vegetation and this protective function became more remarkable if the altitude was higher.And human were reminded to pay close attention to the preservation and protection function of sacred natural sites to natural ecological system at high altitude.展开更多
Two protected sites located on the outskirts of the Sena Oura National Park (PNSO) in West Mayo-Kebbi cover an area of 1800 m2 is pattern choose in pastoral enclave in both village. This study was undertaken to highli...Two protected sites located on the outskirts of the Sena Oura National Park (PNSO) in West Mayo-Kebbi cover an area of 1800 m2 is pattern choose in pastoral enclave in both village. This study was undertaken to highlight the floristic diversity of the herbaceous and woody vegetation on these sites in the offing to know the ability of charge into UBT that most support the park peripheral. It took place on two experimental sites on a natural course in two villages: Wazetelan and Massang. The approach used for the study was a survey using the systematic sampling method and a 30 m × 30 m plot. The survey consisted in listing all the taxa in a floristically homogeneous plot, each assigned the Braun-Blanquet dominance abundance coefficient. These surveys revealed a floristic richness of 73 species, 58 genera, including 46 herbaceous and 26 woody species. The Shannon result gives H1' = 0.12 bit for herbaceous species and H2' = 0.44 bit for woody species, meaning that the herbaceous and woody populations of all the surveys have a very low species diversity, as H' < 3 according to the Shannon index assessment threshold. Herbaceous species are divided into 13 families and 33 genera. The most represented families are Fabaceae (27.3%) and Poaceae (21.9%). Most of the other families (1.3%) have only one species, if any. Herbaceous species are divided into 33 genera grouped into 13 families. The most represented families are Fabaces (16 species), 34%, and Poaceae (12 species), 26%. The 26 woody species, most of which come from itinerant surveys, are distributed across 24 genera and 12 families, the most important of which is Fabaceae with 09 species (34.6%). This floristic assessment, in terms of quantity and quality, has enabled us to estimate the carrying capacity of the two pastoral enclaves in Dari and Goumadji cantons, and to guide the government’s actions with regard to rangeland management.展开更多
Via field survey and analysis,the problems that authenticity of Yuanmingyuan site protection is nibbled by modern garden engineering,and Yuanmingyuan site display has low identification degree,insufficient historical ...Via field survey and analysis,the problems that authenticity of Yuanmingyuan site protection is nibbled by modern garden engineering,and Yuanmingyuan site display has low identification degree,insufficient historical information and improper construction site piece display are elaborated. Meanwhile,it is proposed locating Yuanmingyuan site protection and display into the residual landscape after burnt and looted in 1860. Virtual reality technology is used for full-landscape coverage,to realize the comparison between history and status quo,and the dissemination of Yuanmingyuan information is expanded by"internet+"model,which aims to impel the public joining in heritage awareness and cultural relic preservation.展开更多
In order to explore the protection and renewal law of historic sites in the process of contemporary urban construction, combined with the protection and renewal practice of Qujiang garden cultural landscape heritage s...In order to explore the protection and renewal law of historic sites in the process of contemporary urban construction, combined with the protection and renewal practice of Qujiang garden cultural landscape heritage sites in ancient Chang'an, this paper analyzed the authenticity in the protection and renewal of different historic sites, and explored the possibility of flexible use of the concept of authenticity in the process of protection and renewal of historic sites.展开更多
The protection and utilization of great sites not only are related to the inheritance of historical and cultural heritage and academic research,but also directly affect local economic development and social and cultur...The protection and utilization of great sites not only are related to the inheritance of historical and cultural heritage and academic research,but also directly affect local economic development and social and cultural dissemination.In this paper,five major great sites in Zhengzhou City(Dahe Village Site,Zhengzhou Shangcheng Site,Xiaoshuangqiao Site,the Capital of Zheng and Han States,and Mausoleum of Song Dynasty Tombs of Song Emperors)are taken as research objects,and a set of index system for evaluating the protection and utilization of great sites is constructed.A comprehensive evaluation is conducted from seven aspects:protection management,research value,site utilization,cultural value dissemination,socio-economic benefits,utilization effects,and regional development coordination.This paper provides a comprehensive and scientific evaluation perspective for the protection and utilization of great sites,which helps to better grasp the direction of site protection and utilization,and develop more practical and effective strategies for site protection and utilization.展开更多
In the context of territorial spatial planning,the overall coordinated protection has become a consensus in the field of cultural heritage protection,and the study on the spatial and temporal pattern of trans-regional...In the context of territorial spatial planning,the overall coordinated protection has become a consensus in the field of cultural heritage protection,and the study on the spatial and temporal pattern of trans-regional cultural heritage is of great significance to the overall protection.Based on the cultural relics protection units in Jingzhou area of national great site protection,the temporal and spatial distribution characteristics and influencing factors of cultural heritage resources were studied with the help of ArcGIS.The data show that on the whole,the cultural heritage in Jingzhou area of national great site protection presented the time series of “a large span,more at two ends” and the spatial distribution pattern of “one main,two secondary and three cores”.Cultural heritage in different historical periods had the characteristics of differentiated regional aggregation.The formation of the spatial pattern of different types of cultural heritage was closely related to the local historical and cultural background.Cultural heritage was affected by rivers,terrain,transportation,land,culture and other factors.It was mainly distributed along rivers in areas with little topographic fluctuation and low altitudes.The development and construction of cultural heritage sites were weak,and the ecological foundation was poor.However,the traffic accessibility was good,and the cultural heritage was deep.The research results are expected to provide a scientific basis for guiding the large site protection area to strengthen the integrated protection of cultural resources.展开更多
In the current rapid development of China's environmental protection cause, the construction and control work of environmental protection projects in various parts of the country are becoming more and stricter. Th...In the current rapid development of China's environmental protection cause, the construction and control work of environmental protection projects in various parts of the country are becoming more and stricter. Through the air monitoring of environmental protection projects, the actual operation value of environmental protection project construction can be well improved. However, there are still many external influencing factors in the field work of air monitoring, which have a negative impact on the quality of the overall monitoring work. Therefore, it is necessary to actively do a good job in the air monitoring of environmental protection projects. So as to ensure that the results of air monitoring can reflect the actual conditions of the atmosphere in the region, actively carry out the air monitoring and control work of environmental protection projects, enable the air monitoring work to be carried out to the maximum extent, and provide more help for the value of environmental projects. However, in the current air monitoring work of environmental protection projects, there are various problems, which have brought serious adverse effects to the overall air monitoring work of the project. Therefore, it is necessary to pay attention to the attention of relevant quality control staff, and to improve the quality of the air monitoring site of environmental protection projects.展开更多
The author, in a series of previous articles, designed the “AB Dome” made of transparent thin film supported by a small additional air overpressure for the purpose of covering a city or other important large install...The author, in a series of previous articles, designed the “AB Dome” made of transparent thin film supported by a small additional air overpressure for the purpose of covering a city or other important large installations or sub-regions. In present article the author offers a variation in which a damaged nuclear station can be quickly covered by such a cheap inflatable dome. By containing the radioactive dust from the damaged nuclear station, the danger zone is reduced to about 2 km2 rather than large regions which requires the resettlement of huge masses of people and which stops indus-try in large areas. If there is a big city (as Tokyo) near the nuclear disaster or there is already a dangerous amount of radioactive dust near a city, the city may also be covered by a large inflatable transparent Dome. The building of a gi-gantic inflatable AB Dome over an empty flat surface is not difficult. The cover is spread on a flat surface and a venti-lator (fan system) pumps air under the film cover and lifts the new dome into place but inflation takes many hours. However, to cover a city, garden, forest or other obstacle course in contrast to an empty, mowed field, the thin film cannot be easily deployed over building or trees without risking damage to it by snagging and other complications. This article proposes a new method which solves this problem. The design is a double film blanket filled by light gas such as, methane, hydrogen, or helium - although of these, methane will be the most practical and least likely to leak. Sections of this AB Blanket are lighter than air and will rise in the atmosphere. They can be made on a flat area serving as an as-sembly area and delivered by dirigible or helicopter to station at altitude over the city. Here they connect to the already assembled AB Blanket subassemblies, cover the city in an AB Dome and protect it from bad weather, chemical, bio-logical and radioactive fallout or particulates. After assembly of the dome is completed, the light gas can be replaced by (heavier but cheaper) air. Two projects for Tokyo (Japan) and Moscow (Russia) are used in this paper for sample computation.展开更多
Land for protective forest on the coast has special site conditions, and site classification is the scientific basis for seaboard afforestation. The site classification system on the coast zone and islands of China ma...Land for protective forest on the coast has special site conditions, and site classification is the scientific basis for seaboard afforestation. The site classification system on the coast zone and islands of China may be classified into five levels-site region (sub - region), district, class, group, and type. The land division for afforestation is carried out by the principle of enviornmental heterogeneity among regions, sub-region and district on large scale, according to the difference of air temperature, moisture and type of coast geomorphy. It may be classified into 7 regions, 12 sub-regions and 55 districts. The medium and small scaled division for site class, group and type, subdivided in a site district, are based on medium topography, topographic climate, micro - relief and soil conditions.展开更多
CAS scientists have worked out a plan to further protect the Peking Man site at Zhoukoudian (ZKD) in Beijing, and to renovate research facilities at this internationally renowned paleoanthropological and paleolith... CAS scientists have worked out a plan to further protect the Peking Man site at Zhoukoudian (ZKD) in Beijing, and to renovate research facilities at this internationally renowned paleoanthropological and paleolithic location.……展开更多
Avian metapneumovirus(aMPV),a paramyxovirus,causes acute respiratory diseases in turkeys and swollen head syndrome in chickens.This study established a reverse genetics system for aMPV subtype B LN16-A strain based on...Avian metapneumovirus(aMPV),a paramyxovirus,causes acute respiratory diseases in turkeys and swollen head syndrome in chickens.This study established a reverse genetics system for aMPV subtype B LN16-A strain based on T7 RNA polymerase.Full-length cDNA of the LN16-A strain was constructed by assembling 5 cDNA fragments between the T7 promoter and hepatitis delta virus ribozyme.Transfection of this plasmid,along with the supporting plasmids encoding the N,P,M2-1,and L proteins of LN16-A into BSR-T7/5 cells,resulted in the recovery of aMPV subtype B.To identify an effective insertion site,the enhanced green fluorescent protein(EGFP)gene was inserted into different sites of the LN16-A genome to generate recombinant LN16-As.The results showed that the expression levels of EGFP at the site between the G and L genes of LN16-A were significantly higher than those at the other two sites(between the leader and N genes or replacing the SH gene).To verify the availability of the site between G and L for foreign gene expression,the VP2 gene of very virulent infectious bursal disease virus(vvIBDV)was inserted into this site,and recombinant LN16-A(rLN16A-vvVP2)was successfully rescued.Single immunization of specificpathogen-free chickens with rLN16A-vvVP2 induced high levels of neutralizing antibodies and provided 100%protection against the virulent aMPV subtype B and vvIBDV.Establishing a reverse genetics system here provides an important foundation for understanding aMPV pathogenesis and developing novel vector vaccines.展开更多
文摘The orientation of the biological molecule immobi-lized on a solid surface has been critical in devel-opment of various applications. In this study, ori-entation of antibody was retained by protecting the antigen-binding site of the antibody prior to immo-bilization to -functionalized mixed self-assembled monolayer (SAM) of 12-mercaptododecanoic acid and 1-heptanethiol. More importantly, the number of immobilization bonds formed between each an-tigen-binding site protected antibody molecule and the solid surface was controlled by optimizing the mole fraction of the activated carboxyl group of the linker molecules in the mixed SAM. The amount of antibody used in this study was approximately equivalent to the amount for one monolayer surface coverage. The resulting activity of protected immo-bilized antibody was about 10 fold higher than that of random immobilized
基金Supported by Key Project of Natural Science of Sichuan Province Office of Education(2003A183)Key Project of Natural Science of Liangshan Prefecture Science Commission in 2004(Liangshan Prefecture Science Bureau[2004]46J)~~
文摘With Mosuo's sacred natural sites of mountainous forest in Yongning-Lugu Lake area as the research subject,by using the method of ecology,the vegetation type in this area was initially identified.The control sample plot of the same vegetation type at similar altitude and in similar soil condition would be selected and compared with the sample plot in sacred natural sites at length,so as to quantitatively analyze the protection of biological diversity in the perspective of ecological system.It showed that under the circumstance of the damage of ecological environment,complete natural vertical pattern was preserved thanks to sacred natural sites of mountainous forest established by reason of traditional religion and culture.The results of research and quantitative analysis showed that compared with the control sample plot of the same vegetation type,sacred natural sites were high in biological diversity and the species composition of plant community in sacred natural sites was closer to that of primitive forest.The result of quantitative analysis also indicated that sacred natural sites played a remarkable role in protecting vegetation and this protective function became more remarkable if the altitude was higher.And human were reminded to pay close attention to the preservation and protection function of sacred natural sites to natural ecological system at high altitude.
文摘Two protected sites located on the outskirts of the Sena Oura National Park (PNSO) in West Mayo-Kebbi cover an area of 1800 m2 is pattern choose in pastoral enclave in both village. This study was undertaken to highlight the floristic diversity of the herbaceous and woody vegetation on these sites in the offing to know the ability of charge into UBT that most support the park peripheral. It took place on two experimental sites on a natural course in two villages: Wazetelan and Massang. The approach used for the study was a survey using the systematic sampling method and a 30 m × 30 m plot. The survey consisted in listing all the taxa in a floristically homogeneous plot, each assigned the Braun-Blanquet dominance abundance coefficient. These surveys revealed a floristic richness of 73 species, 58 genera, including 46 herbaceous and 26 woody species. The Shannon result gives H1' = 0.12 bit for herbaceous species and H2' = 0.44 bit for woody species, meaning that the herbaceous and woody populations of all the surveys have a very low species diversity, as H' < 3 according to the Shannon index assessment threshold. Herbaceous species are divided into 13 families and 33 genera. The most represented families are Fabaceae (27.3%) and Poaceae (21.9%). Most of the other families (1.3%) have only one species, if any. Herbaceous species are divided into 33 genera grouped into 13 families. The most represented families are Fabaces (16 species), 34%, and Poaceae (12 species), 26%. The 26 woody species, most of which come from itinerant surveys, are distributed across 24 genera and 12 families, the most important of which is Fabaceae with 09 species (34.6%). This floristic assessment, in terms of quantity and quality, has enabled us to estimate the carrying capacity of the two pastoral enclaves in Dari and Goumadji cantons, and to guide the government’s actions with regard to rangeland management.
文摘Via field survey and analysis,the problems that authenticity of Yuanmingyuan site protection is nibbled by modern garden engineering,and Yuanmingyuan site display has low identification degree,insufficient historical information and improper construction site piece display are elaborated. Meanwhile,it is proposed locating Yuanmingyuan site protection and display into the residual landscape after burnt and looted in 1860. Virtual reality technology is used for full-landscape coverage,to realize the comparison between history and status quo,and the dissemination of Yuanmingyuan information is expanded by"internet+"model,which aims to impel the public joining in heritage awareness and cultural relic preservation.
基金Sponsored by the Fundamental Research Fund for Central Universities(2452018137 2452018138)
文摘In order to explore the protection and renewal law of historic sites in the process of contemporary urban construction, combined with the protection and renewal practice of Qujiang garden cultural landscape heritage sites in ancient Chang'an, this paper analyzed the authenticity in the protection and renewal of different historic sites, and explored the possibility of flexible use of the concept of authenticity in the process of protection and renewal of historic sites.
文摘The protection and utilization of great sites not only are related to the inheritance of historical and cultural heritage and academic research,but also directly affect local economic development and social and cultural dissemination.In this paper,five major great sites in Zhengzhou City(Dahe Village Site,Zhengzhou Shangcheng Site,Xiaoshuangqiao Site,the Capital of Zheng and Han States,and Mausoleum of Song Dynasty Tombs of Song Emperors)are taken as research objects,and a set of index system for evaluating the protection and utilization of great sites is constructed.A comprehensive evaluation is conducted from seven aspects:protection management,research value,site utilization,cultural value dissemination,socio-economic benefits,utilization effects,and regional development coordination.This paper provides a comprehensive and scientific evaluation perspective for the protection and utilization of great sites,which helps to better grasp the direction of site protection and utilization,and develop more practical and effective strategies for site protection and utilization.
基金Sponsored by the Project for the Philosophy and Social Science Research of Hubei Provincial Department of Education in 2021 (21Q049)Innovation and Entrepreneurship Project for University Students of Changjiang University in 2021 (Yz2021325)。
文摘In the context of territorial spatial planning,the overall coordinated protection has become a consensus in the field of cultural heritage protection,and the study on the spatial and temporal pattern of trans-regional cultural heritage is of great significance to the overall protection.Based on the cultural relics protection units in Jingzhou area of national great site protection,the temporal and spatial distribution characteristics and influencing factors of cultural heritage resources were studied with the help of ArcGIS.The data show that on the whole,the cultural heritage in Jingzhou area of national great site protection presented the time series of “a large span,more at two ends” and the spatial distribution pattern of “one main,two secondary and three cores”.Cultural heritage in different historical periods had the characteristics of differentiated regional aggregation.The formation of the spatial pattern of different types of cultural heritage was closely related to the local historical and cultural background.Cultural heritage was affected by rivers,terrain,transportation,land,culture and other factors.It was mainly distributed along rivers in areas with little topographic fluctuation and low altitudes.The development and construction of cultural heritage sites were weak,and the ecological foundation was poor.However,the traffic accessibility was good,and the cultural heritage was deep.The research results are expected to provide a scientific basis for guiding the large site protection area to strengthen the integrated protection of cultural resources.
文摘In the current rapid development of China's environmental protection cause, the construction and control work of environmental protection projects in various parts of the country are becoming more and stricter. Through the air monitoring of environmental protection projects, the actual operation value of environmental protection project construction can be well improved. However, there are still many external influencing factors in the field work of air monitoring, which have a negative impact on the quality of the overall monitoring work. Therefore, it is necessary to actively do a good job in the air monitoring of environmental protection projects. So as to ensure that the results of air monitoring can reflect the actual conditions of the atmosphere in the region, actively carry out the air monitoring and control work of environmental protection projects, enable the air monitoring work to be carried out to the maximum extent, and provide more help for the value of environmental projects. However, in the current air monitoring work of environmental protection projects, there are various problems, which have brought serious adverse effects to the overall air monitoring work of the project. Therefore, it is necessary to pay attention to the attention of relevant quality control staff, and to improve the quality of the air monitoring site of environmental protection projects.
文摘The author, in a series of previous articles, designed the “AB Dome” made of transparent thin film supported by a small additional air overpressure for the purpose of covering a city or other important large installations or sub-regions. In present article the author offers a variation in which a damaged nuclear station can be quickly covered by such a cheap inflatable dome. By containing the radioactive dust from the damaged nuclear station, the danger zone is reduced to about 2 km2 rather than large regions which requires the resettlement of huge masses of people and which stops indus-try in large areas. If there is a big city (as Tokyo) near the nuclear disaster or there is already a dangerous amount of radioactive dust near a city, the city may also be covered by a large inflatable transparent Dome. The building of a gi-gantic inflatable AB Dome over an empty flat surface is not difficult. The cover is spread on a flat surface and a venti-lator (fan system) pumps air under the film cover and lifts the new dome into place but inflation takes many hours. However, to cover a city, garden, forest or other obstacle course in contrast to an empty, mowed field, the thin film cannot be easily deployed over building or trees without risking damage to it by snagging and other complications. This article proposes a new method which solves this problem. The design is a double film blanket filled by light gas such as, methane, hydrogen, or helium - although of these, methane will be the most practical and least likely to leak. Sections of this AB Blanket are lighter than air and will rise in the atmosphere. They can be made on a flat area serving as an as-sembly area and delivered by dirigible or helicopter to station at altitude over the city. Here they connect to the already assembled AB Blanket subassemblies, cover the city in an AB Dome and protect it from bad weather, chemical, bio-logical and radioactive fallout or particulates. After assembly of the dome is completed, the light gas can be replaced by (heavier but cheaper) air. Two projects for Tokyo (Japan) and Moscow (Russia) are used in this paper for sample computation.
文摘Land for protective forest on the coast has special site conditions, and site classification is the scientific basis for seaboard afforestation. The site classification system on the coast zone and islands of China may be classified into five levels-site region (sub - region), district, class, group, and type. The land division for afforestation is carried out by the principle of enviornmental heterogeneity among regions, sub-region and district on large scale, according to the difference of air temperature, moisture and type of coast geomorphy. It may be classified into 7 regions, 12 sub-regions and 55 districts. The medium and small scaled division for site class, group and type, subdivided in a site district, are based on medium topography, topographic climate, micro - relief and soil conditions.
文摘 CAS scientists have worked out a plan to further protect the Peking Man site at Zhoukoudian (ZKD) in Beijing, and to renovate research facilities at this internationally renowned paleoanthropological and paleolithic location.……
基金supported by the grants from the National Key Research and Development Program of China(2022YFD1800604)the China Agriculture Research System(CARS-41)the Heilongjiang Touyan Innovation Team Program,China。
文摘Avian metapneumovirus(aMPV),a paramyxovirus,causes acute respiratory diseases in turkeys and swollen head syndrome in chickens.This study established a reverse genetics system for aMPV subtype B LN16-A strain based on T7 RNA polymerase.Full-length cDNA of the LN16-A strain was constructed by assembling 5 cDNA fragments between the T7 promoter and hepatitis delta virus ribozyme.Transfection of this plasmid,along with the supporting plasmids encoding the N,P,M2-1,and L proteins of LN16-A into BSR-T7/5 cells,resulted in the recovery of aMPV subtype B.To identify an effective insertion site,the enhanced green fluorescent protein(EGFP)gene was inserted into different sites of the LN16-A genome to generate recombinant LN16-As.The results showed that the expression levels of EGFP at the site between the G and L genes of LN16-A were significantly higher than those at the other two sites(between the leader and N genes or replacing the SH gene).To verify the availability of the site between G and L for foreign gene expression,the VP2 gene of very virulent infectious bursal disease virus(vvIBDV)was inserted into this site,and recombinant LN16-A(rLN16A-vvVP2)was successfully rescued.Single immunization of specificpathogen-free chickens with rLN16A-vvVP2 induced high levels of neutralizing antibodies and provided 100%protection against the virulent aMPV subtype B and vvIBDV.Establishing a reverse genetics system here provides an important foundation for understanding aMPV pathogenesis and developing novel vector vaccines.
