Objective: To investigate the association of Graves’ disease and Graves’ ophthalmopathy with the C/T transition polymorphism at position –318 of promoter and the A/G transition polymorphism at position 49 of exon 1...Objective: To investigate the association of Graves’ disease and Graves’ ophthalmopathy with the C/T transition polymorphism at position –318 of promoter and the A/G transition polymorphism at position 49 of exon 1 within cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene. Methods: Thirty-three patients with ophthalmopathy of Graves’ disease, fifty-six Graves’ patients without ophthalmopathy and sixty normal subjects as control were involved in the present case-control study. The polymorphisms were evaluated by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Com-parisons were made of gene frequencies and allele frequencies between the groups. Results: The gene frequencies of CT and allele frequencies of T were much higher in Graves’ patients with ophthalmopathy than that in the group without ophthalmopathy (P=0.020, P=0.019). The gene frequencies of GG and allele frequencies of G in patients with Graves’ disease were significantly increased as compared with control group (P=0.008, P=0.007). The data suggest that smokers with Graves’ disease seemed to be more predisposed to ophthalmopathy than non-smokers (P=0.018). Conclusion: Our results suggest that an allele of T at position –318 of promoter is associated with genetic susceptibility to Graves’ ophthalmopathy while an allele of G at position 49 of exon 1 is associated with genetic susceptibility to Graves’ disease instead. Smoking is believed to be a major risk factor for ophthalmo-pathy.展开更多
Objective： Previous studies have investigated the role of cytotoxic T-lymphocyte antigen-4 （CTLA-4） and tumor necrosis factor-alpha （TNF-a） in carcinogenesis of osteosarcoma, but their results were inconsistent. ...Objective： Previous studies have investigated the role of cytotoxic T-lymphocyte antigen-4 （CTLA-4） and tumor necrosis factor-alpha （TNF-a） in carcinogenesis of osteosarcoma, but their results were inconsistent. We aimed to clarify the associations between CTLA-4, TNF-a polymorphism and osteosarcoma risk by using meta-analysis. Methods： We searched relevant studies without language restriction in PubMed, EMbase, Cochrane Library, Google Scholar databases, Chinese National Knowledge Infrastructure （CNKI） and conference literature in humans published prior to March 2013. The strengths of the associations between genetic variants and osteosarcoma risk were estimated by odds ratio （OR） with 95% confidence interval （95% CI）. Results： A total of seven studies with 1,198 osteosarcoma patients and 1,493 controls were selected. Four studies were eligible for CTLA-4 （1,003 osteosarcoma and 1,162 controls）, and three studies for TNF-a （195 osteosarcoma and 331 controls）. Pooled results showed that rs231775 polymorphism of CTLA-4 was associated with osteosarcoma risk （GG vs. AA： OR=1.63, 95% CI=1.24-2.13; GG ＋ GA vs. AA： OR=1.56, 95% CI=1.21-2.01; AA ＋ GA vs. GG： OR=0.83, 95% CI=0.71-0.97; G vs. A： OR=1.21, 95% CI=1.08-1.36）. No significant heterogeneity was observed across the studies. No significant associations were found between rs5742909 polymorphism of CTLA-4 or rs1800629 polymorphism of TNF-a and osteosarcoma risk. Conclusions： These results suggest that the rs231775 polymorphism of CTLA-4 may play an important role in carcinogenesis of osteosarcoma.展开更多
Aim:The aim was to evaluate the potential infl uences of cytotoxic T-lymphocyte antigen-4(CTLA-4)gene polymorphisms on breast cancer risk,the distribution of CTLA-4 single nucleotide polymorphisms(1661AG)in breast can...Aim:The aim was to evaluate the potential infl uences of cytotoxic T-lymphocyte antigen-4(CTLA-4)gene polymorphisms on breast cancer risk,the distribution of CTLA-4 single nucleotide polymorphisms(1661AG)in breast cancer patients and control subjects was investigated.Methods:In this case-control study,100 patients with breast cancer as case group and 100 healthy participants as a control group were compared.Genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism method.Demographic characteristics of the study population,as well as tumor size,tumor grade and stage were collected in a questionnaire designed for this study.The collected data were statistically analyzed by SPSS-16.0(SPSS Inc.,Chicago,USA)predictive analytic software using the Chi-square test.Results:The mean age of women was 43.42±13.1 years.The AA genotype was frequent in case group(43%)whereas the AG genotype was found more in the control group(69%).There was no signifi cant relationship between the studied polymorphisms and the grade,stage and size of the tumor,nor between the studied polymorphisms and estrogen receptor,progesterone receptor and lymph node involvement(P>0.05).Signifi cant association between the studied polymorphisms and breast cancer metastases was found(P=0.02).Conclusion:According to the results of the study,the AA genotype is associated with breast cancer,but none of the studied gene polymorphisms is associated with prognostic factors such as tumor stage,grade or size.展开更多
Objective To investigate the correlation between gene polymorphism of cytotoxic T lymphocyte associated antigen-4(CTLA-4)and Graves disease(GD)in Qinghai Tibetan.Methods Using retrospective analysis methods,totally 13...Objective To investigate the correlation between gene polymorphism of cytotoxic T lymphocyte associated antigen-4(CTLA-4)and Graves disease(GD)in Qinghai Tibetan.Methods Using retrospective analysis methods,totally 130 cases of GD were selected randomly from June 2012 to November 2016 in the People's Hospital of Qinghai Province;meanwhile,110 normal control cases were selected randomly from Qinghai Tibetan.Then the genotype and allele of CTLA-4 were detected by the method of polymerase chain reaction restriction fragment length polymorphism(RFLP-PCR).Results The distribution of CTLA-4 genotype frequencies(AA,AG,GG)was different between the normal control cases and GD in Qinghai Tibetan [6.2%(8/130)vs 26.4%(29/110),50.0%(65/130)vs 58.2%(64/110),43.8%(57/130)vs 15.4%(17/110),X^2=32.105,P<0.05].Allele(A,G)frequencies were compared between GD and control,the differences were statistically significant [31.2%(81/260)vs 55.5%(122/220),68.8%(179/260)vs 44.5%(98/220),X^2=28.834,P<0.05].Conclusion Polymorphisms of CTLA-4 exon 1(49A/G)genotype and allele are closely correlated with GD in Qinghai Tibetan.展开更多
目的探索肝细胞癌(HCC)患者外周血程序性细胞死亡受体1(programmed death-1,PD-1)、细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、Th1/Th2及其相关细胞因子水平,并分析其与肿瘤标志物甲胎蛋白(alpha...目的探索肝细胞癌(HCC)患者外周血程序性细胞死亡受体1(programmed death-1,PD-1)、细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、Th1/Th2及其相关细胞因子水平,并分析其与肿瘤标志物甲胎蛋白(alpha fetoprotein,AFP)、异常凝血酶原(protein induced by vitamin K absence or antagonistⅡ,PIVKAⅡ)的相关性。方法选择2021年1月至2022年12月简阳市中医医院收治的50例HCC患者作为HCC组,选择同期行健康体检者作为对照组。采用酶联免疫吸附法测定测定外周血AFP、PIVKAⅡ、CTLA-4、PD-1、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ干扰素(interferonγ,IFN-γ)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-10(interleukin-10,IL-10)的水平。采用酶联免疫斑点法测定外周血Th1/Th2。比较两组患者上述指标差异,并采用Pearson法将上述指标与HCC组患者AFP和PIVKAⅡ水平进行相关性分析。结果与对照组相比,HCC组患者外周血TNF-α、IFN-γ及Th1/Th2细胞比例明显下降,而AFP、PIVKAⅡ、IL-6、IL-10、CTLA-4和PD-1水平则明显升高,差异有统计学意义(P<0.05)。相关性分析表明,Th1/Th2与AFP和PIVKA负相关性最高,而PD-1与AFP和PIVKA正相关性最高(r>|7|,P<0.05)。结论HCC患者外周血Th1/Th2明显降低,而CTLA-4和PD-1显著升高,且与HCC血清肿瘤标志物AFP和PIVKA具有密切关系,基于上述指标可为HCC的临床诊断和免疫治疗评估提供参考依据。展开更多
文摘Objective: To investigate the association of Graves’ disease and Graves’ ophthalmopathy with the C/T transition polymorphism at position –318 of promoter and the A/G transition polymorphism at position 49 of exon 1 within cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene. Methods: Thirty-three patients with ophthalmopathy of Graves’ disease, fifty-six Graves’ patients without ophthalmopathy and sixty normal subjects as control were involved in the present case-control study. The polymorphisms were evaluated by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Com-parisons were made of gene frequencies and allele frequencies between the groups. Results: The gene frequencies of CT and allele frequencies of T were much higher in Graves’ patients with ophthalmopathy than that in the group without ophthalmopathy (P=0.020, P=0.019). The gene frequencies of GG and allele frequencies of G in patients with Graves’ disease were significantly increased as compared with control group (P=0.008, P=0.007). The data suggest that smokers with Graves’ disease seemed to be more predisposed to ophthalmopathy than non-smokers (P=0.018). Conclusion: Our results suggest that an allele of T at position –318 of promoter is associated with genetic susceptibility to Graves’ ophthalmopathy while an allele of G at position 49 of exon 1 is associated with genetic susceptibility to Graves’ disease instead. Smoking is believed to be a major risk factor for ophthalmo-pathy.
基金supported by National Natural Science Foundation(No.81260315)Foundation of the Education Department of Guangxi Province,China(No.201010LX375)the Foundation of the Nature Science Fund,Guangxi Province,China(No.2012GXNSFBA053121)
文摘Objective： Previous studies have investigated the role of cytotoxic T-lymphocyte antigen-4 （CTLA-4） and tumor necrosis factor-alpha （TNF-a） in carcinogenesis of osteosarcoma, but their results were inconsistent. We aimed to clarify the associations between CTLA-4, TNF-a polymorphism and osteosarcoma risk by using meta-analysis. Methods： We searched relevant studies without language restriction in PubMed, EMbase, Cochrane Library, Google Scholar databases, Chinese National Knowledge Infrastructure （CNKI） and conference literature in humans published prior to March 2013. The strengths of the associations between genetic variants and osteosarcoma risk were estimated by odds ratio （OR） with 95% confidence interval （95% CI）. Results： A total of seven studies with 1,198 osteosarcoma patients and 1,493 controls were selected. Four studies were eligible for CTLA-4 （1,003 osteosarcoma and 1,162 controls）, and three studies for TNF-a （195 osteosarcoma and 331 controls）. Pooled results showed that rs231775 polymorphism of CTLA-4 was associated with osteosarcoma risk （GG vs. AA： OR=1.63, 95% CI=1.24-2.13; GG ＋ GA vs. AA： OR=1.56, 95% CI=1.21-2.01; AA ＋ GA vs. GG： OR=0.83, 95% CI=0.71-0.97; G vs. A： OR=1.21, 95% CI=1.08-1.36）. No significant heterogeneity was observed across the studies. No significant associations were found between rs5742909 polymorphism of CTLA-4 or rs1800629 polymorphism of TNF-a and osteosarcoma risk. Conclusions： These results suggest that the rs231775 polymorphism of CTLA-4 may play an important role in carcinogenesis of osteosarcoma.
文摘Aim:The aim was to evaluate the potential infl uences of cytotoxic T-lymphocyte antigen-4(CTLA-4)gene polymorphisms on breast cancer risk,the distribution of CTLA-4 single nucleotide polymorphisms(1661AG)in breast cancer patients and control subjects was investigated.Methods:In this case-control study,100 patients with breast cancer as case group and 100 healthy participants as a control group were compared.Genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism method.Demographic characteristics of the study population,as well as tumor size,tumor grade and stage were collected in a questionnaire designed for this study.The collected data were statistically analyzed by SPSS-16.0(SPSS Inc.,Chicago,USA)predictive analytic software using the Chi-square test.Results:The mean age of women was 43.42±13.1 years.The AA genotype was frequent in case group(43%)whereas the AG genotype was found more in the control group(69%).There was no signifi cant relationship between the studied polymorphisms and the grade,stage and size of the tumor,nor between the studied polymorphisms and estrogen receptor,progesterone receptor and lymph node involvement(P>0.05).Signifi cant association between the studied polymorphisms and breast cancer metastases was found(P=0.02).Conclusion:According to the results of the study,the AA genotype is associated with breast cancer,but none of the studied gene polymorphisms is associated with prognostic factors such as tumor stage,grade or size.
文摘Objective To investigate the correlation between gene polymorphism of cytotoxic T lymphocyte associated antigen-4(CTLA-4)and Graves disease(GD)in Qinghai Tibetan.Methods Using retrospective analysis methods,totally 130 cases of GD were selected randomly from June 2012 to November 2016 in the People's Hospital of Qinghai Province;meanwhile,110 normal control cases were selected randomly from Qinghai Tibetan.Then the genotype and allele of CTLA-4 were detected by the method of polymerase chain reaction restriction fragment length polymorphism(RFLP-PCR).Results The distribution of CTLA-4 genotype frequencies(AA,AG,GG)was different between the normal control cases and GD in Qinghai Tibetan [6.2%(8/130)vs 26.4%(29/110),50.0%(65/130)vs 58.2%(64/110),43.8%(57/130)vs 15.4%(17/110),X^2=32.105,P<0.05].Allele(A,G)frequencies were compared between GD and control,the differences were statistically significant [31.2%(81/260)vs 55.5%(122/220),68.8%(179/260)vs 44.5%(98/220),X^2=28.834,P<0.05].Conclusion Polymorphisms of CTLA-4 exon 1(49A/G)genotype and allele are closely correlated with GD in Qinghai Tibetan.
文摘目的探索肝细胞癌(HCC)患者外周血程序性细胞死亡受体1(programmed death-1,PD-1)、细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、Th1/Th2及其相关细胞因子水平,并分析其与肿瘤标志物甲胎蛋白(alpha fetoprotein,AFP)、异常凝血酶原(protein induced by vitamin K absence or antagonistⅡ,PIVKAⅡ)的相关性。方法选择2021年1月至2022年12月简阳市中医医院收治的50例HCC患者作为HCC组,选择同期行健康体检者作为对照组。采用酶联免疫吸附法测定测定外周血AFP、PIVKAⅡ、CTLA-4、PD-1、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ干扰素(interferonγ,IFN-γ)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-10(interleukin-10,IL-10)的水平。采用酶联免疫斑点法测定外周血Th1/Th2。比较两组患者上述指标差异,并采用Pearson法将上述指标与HCC组患者AFP和PIVKAⅡ水平进行相关性分析。结果与对照组相比,HCC组患者外周血TNF-α、IFN-γ及Th1/Th2细胞比例明显下降,而AFP、PIVKAⅡ、IL-6、IL-10、CTLA-4和PD-1水平则明显升高,差异有统计学意义(P<0.05)。相关性分析表明,Th1/Th2与AFP和PIVKA负相关性最高,而PD-1与AFP和PIVKA正相关性最高(r>|7|,P<0.05)。结论HCC患者外周血Th1/Th2明显降低,而CTLA-4和PD-1显著升高,且与HCC血清肿瘤标志物AFP和PIVKA具有密切关系,基于上述指标可为HCC的临床诊断和免疫治疗评估提供参考依据。
