Objective:To evaluate the antibacterial and antifeedant activities of dichloromethane,acetone and aqueous extracts of Spilanthes acmella(L.)(S.acmella)Murr against selected bacterial strains and larvae of Leucinodes o...Objective:To evaluate the antibacterial and antifeedant activities of dichloromethane,acetone and aqueous extracts of Spilanthes acmella(L.)(S.acmella)Murr against selected bacterial strains and larvae of Leucinodes orbonalis Guen.Methods:Solvent extracts were tested against pathogenic microbes using disc diffusion method and fruit disc no-choice method for antifeedant activity.Results:The study revealed that dichloromethane extract of S.acmella leaf showed broad spectrum antibacterial activity against all the tested bacteria.Maximum zone of inhibition was observed in dichloromethane leaf extract of S.acmella against Escherichia coli[(18.9±0.34)mm]followed by Staphylococcus aureus[(18.6±1.31)mm],Proteus vulgaris[(17.2±0.68)mm],Bacillus subtilis[(17.0±0.76)mm]and Klebsiella pneumonia[(16.4±1.55)mm]at 5 mg/disc concentration.The aqueous extract was moderately inhibited the tested bacteria at all the concentrations.Dichloromethane extract showed good antifeedant activity against Leucinodes orbonalis(68.88%)when compared to acetone(60.80%)and aqueous(45.48%)extracts at 5%concentration.The preliminary phytochemical analysis showed the presence of alkaloids,terpenoids,phytosterols,saponins,steroid,tannins and phenolic compounds.Conclusions:The study suggests that the dichloromethane leaf extract of S.acmella could be used to develop a novel herbal formulation to control pathogenic bacteria and agricultural pests.展开更多
基金Supported by Department of Science and Technology(DST),Division of Science and Engineering Research Board(SERB)under Fast Track Scheme for Young Scientist Project(Grant No.SERC/LS-0412/2010).
文摘Objective:To evaluate the antibacterial and antifeedant activities of dichloromethane,acetone and aqueous extracts of Spilanthes acmella(L.)(S.acmella)Murr against selected bacterial strains and larvae of Leucinodes orbonalis Guen.Methods:Solvent extracts were tested against pathogenic microbes using disc diffusion method and fruit disc no-choice method for antifeedant activity.Results:The study revealed that dichloromethane extract of S.acmella leaf showed broad spectrum antibacterial activity against all the tested bacteria.Maximum zone of inhibition was observed in dichloromethane leaf extract of S.acmella against Escherichia coli[(18.9±0.34)mm]followed by Staphylococcus aureus[(18.6±1.31)mm],Proteus vulgaris[(17.2±0.68)mm],Bacillus subtilis[(17.0±0.76)mm]and Klebsiella pneumonia[(16.4±1.55)mm]at 5 mg/disc concentration.The aqueous extract was moderately inhibited the tested bacteria at all the concentrations.Dichloromethane extract showed good antifeedant activity against Leucinodes orbonalis(68.88%)when compared to acetone(60.80%)and aqueous(45.48%)extracts at 5%concentration.The preliminary phytochemical analysis showed the presence of alkaloids,terpenoids,phytosterols,saponins,steroid,tannins and phenolic compounds.Conclusions:The study suggests that the dichloromethane leaf extract of S.acmella could be used to develop a novel herbal formulation to control pathogenic bacteria and agricultural pests.
