Antibacterial activities of various spherical zinc oxide nanoparticles and nano special morphological structures including quantum dots, nanorod arrays, nanoporous shapes and needle-like crystals had been investigated...Antibacterial activities of various spherical zinc oxide nanoparticles and nano special morphological structures including quantum dots, nanorod arrays, nanoporous shapes and needle-like crystals had been investigated as new nanomedicine compounds. Also antibacterial activity based on minimal inhibitory concentration and the growth inhibitory zone (well method) was evaluated. ZnO nanostructures were fabricated by novel hydrolysis sol-gel-hydrothermal process followed with rapid quenching as new technique using glycerine, vegetable fatty esters such as coconut, sunflower and Lauric alcohol ethoxylated as organic templates soluble in eco-friendly nanofluids. The results showed that Bacillus anthracis and Pseudomonas aerogenes were extremely sensitive to treatment with unique ZnO nanostructured. Their growth inhibitory zone presented 30 mm and 25 mm inhibition zone with better inhibitory effect compared to the Gentamicin antibiotic standard. ZnO nanostructures had also been indicated to have a wide range of antibacterial activities against both Gram-positive and Gram-negative bacteria especially more effective on (gr+) species using the growth inhibitory zone. We could design and make significant formulations of fatty acids and esters-capped ZnO quantum dots nanofluids which created high promising agents for controlling Anthrax, Staphylococcus epidermidis and their influences in antimicrobial properties with low cost for future.展开更多
The effect of antibacterial adhesive on the biological corrosion resistance of mortar in seawater environment was studied by means of scanning electron microscope,thermogravimetric analysis,X-ray diffraction,Fourier t...The effect of antibacterial adhesive on the biological corrosion resistance of mortar in seawater environment was studied by means of scanning electron microscope,thermogravimetric analysis,X-ray diffraction,Fourier transform infrared spectroscopy,and ultra-depth microscope.The results show that the antibacterial adhesive can effectively inhibit the growth of sulfur-oxidizing bacteria in seawater,hinder their metabolism to produce biological sulfate,and reduce the formation of destructive product gypsum.The mineral composition and thermal analysis showed that the peak value of plaster diffraction peak and the mass loss of plaster dehydration in antibacterial adhesive group were significantly lower than those in blank group(without protective coating group).In addition,the electric flux of chloride ions(>400 C)in the blank group of mortar samples was higher than that in the antibacterial adhesive group(<200 C),indicating that the antibacterial adhesive can effectively reduce the permeability of chloride ions in mortar,and thus hinder the Cl-erosion in seawater.展开更多
Background: Mass drug administration(MDA)is a strategy to improve health at the population level through widespread delivery of medicine in a community.We surveyed the literature to summarize the benefits and potentia...Background: Mass drug administration(MDA)is a strategy to improve health at the population level through widespread delivery of medicine in a community.We surveyed the literature to summarize the benefits and potential risks associated with MDA of antibacterials,focusing predominantly on azithromycin as it has the greatest evidence base.Main body: High-quality evidence from randomized controlled trials(RCTs)indicate that MDA-azithromycin is effective in reducing the prevalence of infection due to yaws and trachoma.In addition,RCTs suggest that MDA-azithromycin reduces under-five mortality in certain low-resource settings that have high childhood mortality rates at baseline.This reduction in mortality appears to be sustained over time with twice-yearly MDA-azithromycin,with the greatest effect observed in children<1 year of age.In addition,observational data suggest that infections such as skin and soft tissue infections,rheumatic heart disease,acute respiratory illness,diarrheal illness,and malaria may all be treated by azithromycin and thus incidentally impacted by MDA-azithromycin.However,the mechanism by which MDA-azithromycin reduces childhood mortality remains unclear.Verbal autopsies performed in MDA-azithromycin childhood mortality studies have produced conflicting data and are underpowered to answer this question.In addition to benefits,there are several important risks associated with MDA-azithromycin.Direct adverse effects potentially resulting from MDA-azithromycin include gastrointestinal side effects,idiopathic hypertrophic pyloric stenosis,cardiovascular side effects,and increase in chronic diseases such as asthma and obesity.Antibacterial resistance is also a risk associated with MDA-azithromycin and has been reported for both gram-positive and enteric organisms.Further,there is the risk for cross-resistance with other antibacterial agents,especially clindamycin.Conclusions: Evidence shows that MDA-azithromycin programs may be beneficial for reducing trachoma,yaws,and mortality in children<5 years of age in certain under-resourced settings.However,there are significant potential risks that need to be considered when deciding how,when,and where to implement these programs.Robust systems to monitor benefits as well as adverse effects and antibacterial resistance are warranted in communities where MDA-azithromycin programs are implemented.展开更多
Silver nanoparticles(Ag NPs)have attracted attention in the field of biomaterials due to their excellent antibacterial property.However,the reducing and stabilizing agents used for the chemical reduction of Ag NPs are...Silver nanoparticles(Ag NPs)have attracted attention in the field of biomaterials due to their excellent antibacterial property.However,the reducing and stabilizing agents used for the chemical reduction of Ag NPs are usually toxic and may cause water pollution.In this work,Ag NPs(31.2 nm in diameter)were prepared using the extract of straw,an agricultural waste,as the reducing and stabilizing agent.Experimental analysis revealed that the straw extract contained lignin,the structure of which possesses phenolic hydroxyl and methoxy groups that facilitate the reduction of silver salts into Ag NPs.The surfaces of Ag NPs were negatively charged due to the encapsulation of a thin layer of lignin molecules that prevented their aggregation.After the prepared Ag NPs were added to the precursor solution of acrylamide,free radical polymerization was triggered without the need for extra heating or light irradiation,resulting in the rapid formation of an Ag NP-polyacrylamide composite hydrogel.The inhibition zone test proved that the composite hydrogel possessed excellent antibacterial ability due to the presence of Ag NPs.The prepared hydrogel may have potential applications in the fabrication of biomedical materials,such as antibacterial dressings.展开更多
The defect regulation and p-n heterojunction of composites have gained significant attention due to their potential applications.Nitrogen(N)as doping heteroatoms and perylene-3,4,9,10-tetracarboximide(PDINH)as an appr...The defect regulation and p-n heterojunction of composites have gained significant attention due to their potential applications.Nitrogen(N)as doping heteroatoms and perylene-3,4,9,10-tetracarboximide(PDINH)as an appropriate n-type semiconductor were innovatively and reasonably selected to enhance the photocatalytic performance of pristine p-type cuprous oxide(Cu_(2)O).In this study,the defect regula-tion of N doping(1)achieved the small-size effect of Cu_(2)O,(2)optimized the electron features,and(3)improved the kinetics of reactive oxygen species.The p-n heterojunction with PDINH was developed to sharply improve the light utilization of Cu_(2)O,from the UV region to the near-infrared region.As expected,the optimized Cu_(2)N_(x)O_(1–x)/PDINH(x=0.02)exhibited excellent long-term photocatalytic antibacterial ac-tivities,with antibacterial rates exceeding 91%against Staphylococcus aureus and Pseudomonas aeruginosa.Defect regulation and p-n heterojunction of Cu_(2)O-based composites thus provide a great deal of potential for future advancements in photocatalysis.展开更多
Nano-zinc oxides(ZnO)demonstrate remarkable antibacterial properties.To further enhance the corrosion resistance and antibacterial efficiency of magnesium alloy micro-arc oxidation(MAO)coatings,this study investigates...Nano-zinc oxides(ZnO)demonstrate remarkable antibacterial properties.To further enhance the corrosion resistance and antibacterial efficiency of magnesium alloy micro-arc oxidation(MAO)coatings,this study investigates the preparation of ZnO-containing micro-arc oxidation coatings with dual functionality by incorporating nano-ZnO into MAO electrolyte.The influence of varying ZnO concentrations on the microstructure,corrosion resistance,and antibacterial properties of the coating was examined through microstructure analysis,immersion tests,electrochemical experiments,and antibacterial assays.The findings revealed that the addition of nano-ZnO significantly enhanced the corrosion resistance of the MAO-coated alloy.Specifically,when the ZnO concentration in the electrolyte was 5 g/L,the corrosion rate was more than ten times lower compared to the MAO coatings without ZnO.Moreover,the antibacterial efficacy of ZnO+MAO coating,prepared with a ZnO concentration of 5 g/L,surpassed 95%after 24 h of co-culturing with Staphylococcus aureus(S.aureus).The nano-ZnO+MAO-coated alloy exhibited exceptional degradation resistance,corrosion resistance,and antibacterial effectiveness.展开更多
Bacterial infection,insufficient angiogenesis,and oxidative damage are generally regarded as key issues that impede wound healing,making it necessary to prepare new biomaterials to simultaneously address these problem...Bacterial infection,insufficient angiogenesis,and oxidative damage are generally regarded as key issues that impede wound healing,making it necessary to prepare new biomaterials to simultaneously address these problems.In this work,monodispersed CeO_(2)@CuS nanocomposites(NCs)were successfully prepared with tannin(TA)as the reductant and linker.Due to abundant oxygen vacancies in CeO_(2)and the polyphenolic structure of TA,the TA-CeO_(2)@CuS NCs exhibited a remarkable antioxidant ability to scavenge excessive reactive oxygen species(ROS),which would likely induce serious inflammation.In addition,the TA-CeO_(2)@CuS NCs demonstrated excellent antibacterial capability with near-infrared ray(NIR)irradiation,and the released copper ions could promote the regeneration of blood vessels.These synergistic effects indicated that the synthesized TA-CeO_(2)@CuS NCs could serve as a promising biomaterial for multimodal wound therapy.展开更多
In clinical practice,antibiotics have historically been utilized for the treatment of pathogenic bacteria.However,the gradual emergence of antibiotic resistance among bacterial strains has posed a significant challeng...In clinical practice,antibiotics have historically been utilized for the treatment of pathogenic bacteria.However,the gradual emergence of antibiotic resistance among bacterial strains has posed a significant challenge to this approach.In 2022,Escherichia coli,a Gram-negative bacterium renowned for its widespread pathogenicity and high virulence,emerged as the predominant pathogenic bacterium in China.The rapid emergence of antibiotic-resistant E.coli strains has rendered antibiotics insufficient to fight E.coli infections.Traditional Chinese medicine(TCM)has made remarkable contributions to the health of Chinese people for thousands of years,and its significant therapeutic effects have been proven in clinical practice.In this paper,we provide a comprehensive review of the advances and mechanisms of TCM and its active ingredients against antibiotic-resistant E.coli infections.First of all,this review introduces the classification,antibiotic resistance characteristics and mechanisms of E.coli.Then,the TCM formulas and extracts are listed along with their active ingredients against E.coli,including extraction solution,minimum inhibitory concentration(MIC),and the antibacterial mechanisms.In addition,there is growing evidence supporting the synergistic therapeutic strategy of combining TCM with antibiotics for the treatment of antibiotic-resistant E.coli infections,and we provide a summary of this evidence and its underlying mechanisms.In conclusion,we present a comprehensive review of TCM and highlight its potential and advantages in the prevention and treatment of E.coli infections.We hold the opinion that TCM will play an important role in global health,pharmaceutical development,and livestock farming in the future.展开更多
Superhydrophobic surface is a promising strategy for antibacterial and corrosion protection.However,the use of harmful fluorine-containing materials,poor mechano-chemical stability,the addition of fungicides and poor ...Superhydrophobic surface is a promising strategy for antibacterial and corrosion protection.However,the use of harmful fluorine-containing materials,poor mechano-chemical stability,the addition of fungicides and poor corrosion resistance often limit its practical application.In this paper,a high-robustness pho-tothermal self-healing superhydrophobic coating is prepared by simply spraying a mixture of hydropho-bically modified epoxy resin and two kinds of modified nanofillers(carbon nanotubes and SiO2)for long-term anticorrosion and antibacterial applications.Multi-scale network and lubrication structures formed by cross-linking of modified carbon nanotubes and repeatable roughness endow coating with high ro-bustness,so that the coating maintains superhydrophobicity even after 100 Taber abrasion cycles,20 m sandpaper abrasion and 100 tape peeling cycles.The synergistic effect of antibacterial adhesion and pho-tothermal bactericidal activity endows coating with excellent antibacterial efficiency,which against Es-cherichia coli(E.coli)and Staphylococcus aureus(S.aureus)separately reaches 99.6% and 99.8%.Moreover,the influence of modified epoxy resin,superhydrophobicity,organic coating and coating thicknesses on the anticorrosion of magnesium(Mg)alloy is systematically studied and analyzed.More importantly,the prepared coating still exhibits excellent self-cleaning,anticorrosion and antibacterial abilities after 20 m abrasion.Furthermore,the coating exhibits excellent adhesion(level 4B),chemical stability,UV radiation resistance,high-low temperature alternation resistance,stable heat production capacity and photother-mal self-healing ability.All these excellent performances can promote its application in a wider range of fields.展开更多
Titanium alloys,usually known as non-corrodible material,are susceptible to microbiologically influenced corrosion(MIC)in marine environment.While titanium-zirconium(TiZr)alloys have been extensively studied in medica...Titanium alloys,usually known as non-corrodible material,are susceptible to microbiologically influenced corrosion(MIC)in marine environment.While titanium-zirconium(TiZr)alloys have been extensively studied in medical applications,the influence of microorganisms,especially marine microorganisms,on their corrosion behavior has not been explored.In this work,a TiZrCu alloy with a combination of excel-lent mechanical,anti-corrosion,and antibacterial properties was developed by optimizing the Cu content and grain refinement.Its MIC and antibacterial mechanisms against Pseudomonas aeruginosa,a represen-tative marine microorganism,were systematically investigated.5.5 wt%was determined as the optimal copper content.The fine-grained Ti-15Zr-5.5Cu(TZC-5.5FG)alloy maintained high MIC resistance,exhibit-ing a corrosion current of 5.7±0.1 nA/cm^(2) and an antibacterial rate of 91.8% against P.aeruginosa.The mechanism of improved corrosion resistance was attributed to the denser passive film with high TiO2 content and the lower surface potential differenceΔE.The release of Cu^(2+)ions,ΔE,and the generation of ROS are three major factors that contribute to the antibacterial performance of TiZrCu alloys.Com-pared to other available marine metals,TZC-5.5FG alloy exhibited superior comprehensive performance,including excellent mechanical properties and anti-MIC capacity,which make it a promising material for load-bearing applications in marine environment.展开更多
Concerns about air quality in dental clinics where aerosol generation during procedures poses significant health risks,have prompted investigations on advanced disinfection technologies.This editorial describes the st...Concerns about air quality in dental clinics where aerosol generation during procedures poses significant health risks,have prompted investigations on advanced disinfection technologies.This editorial describes the strengths and limitations of ventilation and aerosol control measures in dental offices,especially with respect to the use of graphene nanocomposites.The potential of graphene nanocomposites as an innovative solution to aerosol-associated health risks is examined in this review due to the unique properties of graphene(e.g.,high con-ductivity,mechanical strength,and antimicrobial activity).These properties have produced promising results in various fields,but the application of graphene in dentistry remains unexplored.The recent study by Ju et al which was published in World Journal of Clinical Cases evaluated the effectiveness of graphene-based air disinfection systems in dental clinics.The study demonstrated that graphene-based disinfection techniques produced significant reductions in suspended particulate matter and bacterial colony counts,when co-mpared with traditional methods.Despite these positive results,challenges such as material saturation,frequency of filter replacement,and associated costs must be addressed before widespread adoption of graphene-based disinfection techniques in clinical practice.Therefore,there is need for further research on material structure optimization,long-term safety evaluations,and broader clinical applications,in order to maximize their positive impact on public health.展开更多
The combination of solar disinfection and photocatalysis technology presents a viable solution for eliminating harmful pathogenic microorganisms from water.However,some photocatalysts(e.g.,zinc oxide-based composites)...The combination of solar disinfection and photocatalysis technology presents a viable solution for eliminating harmful pathogenic microorganisms from water.However,some photocatalysts(e.g.,zinc oxide-based composites)are susceptible to pH-dependent dissolution in water,which can result in the loss of photocatalysts and additional environ-mental pollution.To obtain zinc oxide-based composites with low dissolution and high antibacterial efficiency for pho-tocatalytic water disinfection,we prepared MoS_(2)/ZnO@CS composites via a precipitation method to encapsulate chitosan(CS)around MoS_(2)/ZnO.The amino groups in the CS molecules act as storerooms for hydrogen ions,which inhibits the dissolution of zinc oxide.In addition,the MoS_(2)/ZnO@CS composites exhibit high production of reactive oxygen species(ROS)and broad-spectrum antibacterial activity under simulated solar irradiation(0.1 W·cm^(-2)).This makes it an excellent antibacterial agent for solar disinfection in water treatment.展开更多
A novel 3D metal-organic framework(MOF)[Pr_(2)(L)_(3)(H_(2)O)5·H_(2)O]n(Pr-1),(H_(2)L=4,4'-oxybis(benzoic acid))with a rare structure of broken layer net,was constructed under the condition of solvothermal sy...A novel 3D metal-organic framework(MOF)[Pr_(2)(L)_(3)(H_(2)O)5·H_(2)O]n(Pr-1),(H_(2)L=4,4'-oxybis(benzoic acid))with a rare structure of broken layer net,was constructed under the condition of solvothermal synthesis.The struc-ture and crystal net were analyzed and characterized.This rod net of Pr-1 is new to both RCSR and ToposPro data-bases,and is named as rn-12 as suggested.Due to the luminescent properties of H_(2)L and Pr(Ⅲ),the solid-state fluo-rescence property and sensing performance(solvents and metal ions)of Pr-1 were investigated.The sensing experi-ments indicated that Pr-1 could act as a fluorescence sensor to detect Cd^(2+)ions with good sensitivity.In addition,antibacterial activities show that Pr-1 exhibited stronger antibacterial activity against Escherichia coli(E.coli),Staphylococcus aureus(S.aureus),and Bacillus subtilis(B.subtilis)compared to synthetic materials.展开更多
In order to study the effects of Saussurea laniceps polysaccharides(SLPs)on the expression of inflammatory factors and antimicrobial peptide LL-37 in UVB-induced keratinocytes,SLPs were extracted by the ethanol therma...In order to study the effects of Saussurea laniceps polysaccharides(SLPs)on the expression of inflammatory factors and antimicrobial peptide LL-37 in UVB-induced keratinocytes,SLPs were extracted by the ethanol thermal reflux method,and SLPs at different concentrations were used to examine the inhibitory effect of COX-2(a key mediator of inflammatory pathway).A cell model of UVB irradiation-induced inflammation was established to determine the influence of SLPs on prostaglandin E2(PGE-2),TNF-αand IL-1βinflammatory factors,as well as the relationships of SLPs with LL-37 expression.An enzyme-linked immunosorbent assay(ELISA)and western blot analysis were used to detect the production of inflammatory factors and LL-37 antimicrobial peptide.The results showed that the inhibition rate of COX-2 was 82.41%at 1000μg/mL,and the expression of PGE-2,TNF-αand IL-1βinflammatory factors in HaCaT cells was significantly downregulated at 100μg/mL(P<0.01).In addition,SLPs at 50μg/mL and 100μg/mL concentrations enhanced the expression of LL-37 antimicrobial peptide(P<0.01),thereby down-regulating the expression of TNF-αand IL-1βinflammatory factors,then reducing skin inflammation.Conclusion:SLP can significantly inhibit the inflammatory response induced by UVB,and can further slow down the damage caused by inflammation to the skin by regulating LL-37 antimicrobial peptides,which has the potential to prevent skin inflammatory damage caused by UVB irradiation.展开更多
Infections associated with titanium(Ti)-based implants present significant challenges in clinical treatments,especially when biofilms already form on the implant surface.Many antimicrobial agents,including antibiotics...Infections associated with titanium(Ti)-based implants present significant challenges in clinical treatments,especially when biofilms already form on the implant surface.Many antimicrobial agents,including antibiotics,metallic nanoparticles and antimicrobial peptides,have been extensively used to deal with Ti implant infections.However,these chemical approaches suffer from potential toxicity,antibiotic resistance and poor long-term antibacterial performance.Hence,physical antibacterial surfaces on Ti-based implants have attracted increasing attention.The antibacterial behavior of different surfaces on Ti-based biomaterials against various bacteria only by physical properties of the implants themselves(e.g.,nanotopography)or exogenous physical stimulus(e.g.,photocatalysis)was reviewed,as well as parameters influencing the physical antibacterial processes,such as size,shape and density of the surface nanotextures,and bacterial growth phases.Besides,mechanisms of different fabrication techniques for the physical antibacterial surfaces on Ti-based biomaterials were also summarized.展开更多
Two Gd_(2)complexes,namely[Gd_(2)(dbm)_(2)(HL_(1))_(2)(CH_(3)OH)_(2)]·4CH_(3)OH(1)and[Gd_(2)(dbm)_(2)(L_(2))_(2)(CH_(3)OH)_(2)]·2CH_(3)OH(2),where H_(3)L_(1)=(Z)-N'-[4-(diethylamino)-2-hydroxybenzylidene...Two Gd_(2)complexes,namely[Gd_(2)(dbm)_(2)(HL_(1))_(2)(CH_(3)OH)_(2)]·4CH_(3)OH(1)and[Gd_(2)(dbm)_(2)(L_(2))_(2)(CH_(3)OH)_(2)]·2CH_(3)OH(2),where H_(3)L_(1)=(Z)-N'-[4-(diethylamino)-2-hydroxybenzylidene]-2-hydroxyacetohydrazide,H_(2)L_(2)=(E)-N'-(5-bromo-2-hydroxy-3-methoxybenzylidene)nicotinohydrazide,Hdbm=dibenzoylmethane,have been constructed by adopting the solvothermal method.Structural characterization unveils that both complexes 1 and 2 are constituted by two Gd^(3+)ions,two dbm-ions,two CH_(3)OH molecules,and two polydentate Schiff-base ligands(HL_(1)^(2-)or L_(2)^(2-)).In addition,complex 1 contains four free methanol molecules,whereas complex 2 harbors two free methanol molecules.By investigating the interactions between complexes 1 and 2 and four types of bacteria(Bacillus subtilis,Escherichia coli,Staphylococcus aureus,Candida albicans),it was found that both complexes 1 and 2 exhibited potent antibacte-rial activities.The interaction mechanisms between the ligands H_(3)L_(1),H_(2)L_(2),complexes 1 and 2,and calf thymus DNA(CT-DNA)were studied using ultraviolet-visible spectroscopy,fluorescence titration,and cyclic voltammetry.The results demonstrated that both complexes 1 and 2 can intercalate into CT-DNA molecules,thereby inhibiting bacterial proliferation to achieve the antibacterial effects.CCDC:2401116,1;2401117,2.展开更多
As a rich source of bioactive compounds,plant-derived natural products hold immense promise for pharmaceutical research and development,offering a path to discovering new drug targets and innovative treatments.Sonchus...As a rich source of bioactive compounds,plant-derived natural products hold immense promise for pharmaceutical research and development,offering a path to discovering new drug targets and innovative treatments.Sonchus species,commonly known as sow thistles,are widely distributed across various geographical regions.Several common species within this plant group have a long history of use in traditional medicine,which can be attributed to their diverse therapeutic benefits.Recently,these plants have gained attention for their potential in antimicrobial therapy,owing to their rich phytochemical profile,including sesquiterpene,flavonoids,and phenolic acids with known antimicrobial activities.Currently,available pharmacological studies demonstrate that Sonchus species extracts possess antimicrobial properties,highlighting their potential as sources for new therapeutic agents.This review provides an overview of the current knowledge on the antibacterial,antifungal,antiparasitic,and antiviral activities of the Sonchus species,along with its possible mechanisms of action.The review aims to provide directions for future research and promote the exploration of these plants as viable sources of natural antimicrobial agents,potentially contributing to the development of alternative therapeutic strategies in the face of rising antibiotic resistance.展开更多
This work uses GC-MS to analyze the bioactive compounds of Salvia rosmarinus essential oils(SREO)and evaluates their antibacterial,antifungal,and insecticidal effects,as well as the major component,1,8-cineole.Chemica...This work uses GC-MS to analyze the bioactive compounds of Salvia rosmarinus essential oils(SREO)and evaluates their antibacterial,antifungal,and insecticidal effects,as well as the major component,1,8-cineole.Chemical analysis identified 16 compounds accounting for 99.19%of the oil’s total content,with 1,8-cineole(33.17%),camphor(16.53%),α-pinene(14.46%),and camphene(8.14%)as the major constituents.Antimicrobial activities were assessed against pathogenic strains using minimal inhibit concentration(MIC)and minimum bactericidal concentration(MBC)assays.SREO exhibited a minimum MIC of 0.128%against P.aeruginosa,while 1,8-cineole showed a minimum MIC of 2.06%against the same strain,highlighting the higher efficacy of the complete oil compared to the isolated compound.Conversely,for antifungal activity,1,8-cineole displayed a lower MIC(2.06%)against A.niger and P.digitatum compared to SREO(4.125%against A.niger).Regarding aphicidal activity,results demonstrated the lethal effects of SREO on M.persicae,with an even more pronounced impact observed for 1,8-cineole.At one dose of 40μL/L air,SREO and 1,8-cineole resulted in 100%insect mortality within 24 h of exposure.After 12 h of exposure to SREO at concentrations of 5,10,20,and 40μL/L air,the mortality rates were 20%,36.67%,70%,and 93.33%.1,8-cineole showed maximum efficacy,achieving complete(100%)mortality within 12 h at 40μL/L air.展开更多
Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the trea...Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses.Therefore,it is impor-tant to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains.In this study,novel selenium-tellurium based nanoparticles(SeTeNPs)were synthesized and characterized.Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model.A total of 10 heifers were divided into experimental and control groups(5 animals each).After intramammary infection with methicillin resistant S.aureus(MRSA)and the development of clinical signs of mastitis,a dose of SeTeNPs was administered to all quarters in the experimental group.Results Based on in vitro tests,the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te,respectively,was used for application into the mammary gland.Three days after SeTeNPs administration,MRSA counts in the experimental group showed a significant reduction(P<0.01)compared to the control group.The inhibitory effect observed within the in vitro experiments was thus confirmed,resulting in the suppression of infection in ani-mals.Moreover,the superior biocompatibility of SeTeNPs in the organism was demonstrated,as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application,i.e.,mammary gland,compared to the control group(P>0.05).Additionally,the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected,indicating that the nanoparticles had no adverse effects on the organism.Conclusions Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the pres-ence of resistant bacteria.However,the current study is limited by its small sample size,making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs.Therefore,further research with a larger sample size is needed to validate these results.展开更多
This study aims to optimize biosurfactant production by Streptomyces gobitricini strain BS-2.After the initial screening,the effective factors on the production of biosurfactant were investigated with the two-level fa...This study aims to optimize biosurfactant production by Streptomyces gobitricini strain BS-2.After the initial screening,the effective factors on the production of biosurfactant were investigated with the two-level factorial statistical.The physical and chemical features and structure of the biosurfactant crafted were analyzed using FTIR,CHNS,EDX,and SEM.The extracted biosurfactant resulted in a decrease in interfacial tension from 72 mN/m to 29 mN/m,with a critical micelle concentration(CMC)of 300 mg/mL.The optimum medium for biosurfactant production that led to utmost diminution in culture broth surface tension(29 mN/m)was:crude oil(1.5%,v/v),hydrolyzed protein(1 g/L),yeast extract(0.5 g/L),molasses(1.5 g/L)and olive oil(1%v/v).The glycolipid organization of the microbial surfactant was confirmed via FTIR,CHNS,EDX,and SEM analysis.Analysis using GC indicated that the presence of biosurfactant increased crude oil degradation by S.gobitricini strain BS-2 dramatically(87%)and this bacterium effectively degraded most of its alkane components.Furthermore,the glycolipid biosurfactant displayed significant development repression against therapeutic bacterial microbes.In summary,the glycolipid biosurfactant produced by S.gobitricini strain BS-2 exhibited diverse functional properties and showed promise for potential biomedical and biotechnological applications.展开更多
文摘Antibacterial activities of various spherical zinc oxide nanoparticles and nano special morphological structures including quantum dots, nanorod arrays, nanoporous shapes and needle-like crystals had been investigated as new nanomedicine compounds. Also antibacterial activity based on minimal inhibitory concentration and the growth inhibitory zone (well method) was evaluated. ZnO nanostructures were fabricated by novel hydrolysis sol-gel-hydrothermal process followed with rapid quenching as new technique using glycerine, vegetable fatty esters such as coconut, sunflower and Lauric alcohol ethoxylated as organic templates soluble in eco-friendly nanofluids. The results showed that Bacillus anthracis and Pseudomonas aerogenes were extremely sensitive to treatment with unique ZnO nanostructured. Their growth inhibitory zone presented 30 mm and 25 mm inhibition zone with better inhibitory effect compared to the Gentamicin antibiotic standard. ZnO nanostructures had also been indicated to have a wide range of antibacterial activities against both Gram-positive and Gram-negative bacteria especially more effective on (gr+) species using the growth inhibitory zone. We could design and make significant formulations of fatty acids and esters-capped ZnO quantum dots nanofluids which created high promising agents for controlling Anthrax, Staphylococcus epidermidis and their influences in antimicrobial properties with low cost for future.
基金Funded by the National Natural Science Foundation of China(Nos.52278269,52278268,52178264)Tianjin Outstanding Young Scholars Science Fund Project(No.22JCJQJC00020)Key Project of Tianjin Natural Science Foundation(No.23JCZDJC00430)。
文摘The effect of antibacterial adhesive on the biological corrosion resistance of mortar in seawater environment was studied by means of scanning electron microscope,thermogravimetric analysis,X-ray diffraction,Fourier transform infrared spectroscopy,and ultra-depth microscope.The results show that the antibacterial adhesive can effectively inhibit the growth of sulfur-oxidizing bacteria in seawater,hinder their metabolism to produce biological sulfate,and reduce the formation of destructive product gypsum.The mineral composition and thermal analysis showed that the peak value of plaster diffraction peak and the mass loss of plaster dehydration in antibacterial adhesive group were significantly lower than those in blank group(without protective coating group).In addition,the electric flux of chloride ions(>400 C)in the blank group of mortar samples was higher than that in the antibacterial adhesive group(<200 C),indicating that the antibacterial adhesive can effectively reduce the permeability of chloride ions in mortar,and thus hinder the Cl-erosion in seawater.
文摘Background: Mass drug administration(MDA)is a strategy to improve health at the population level through widespread delivery of medicine in a community.We surveyed the literature to summarize the benefits and potential risks associated with MDA of antibacterials,focusing predominantly on azithromycin as it has the greatest evidence base.Main body: High-quality evidence from randomized controlled trials(RCTs)indicate that MDA-azithromycin is effective in reducing the prevalence of infection due to yaws and trachoma.In addition,RCTs suggest that MDA-azithromycin reduces under-five mortality in certain low-resource settings that have high childhood mortality rates at baseline.This reduction in mortality appears to be sustained over time with twice-yearly MDA-azithromycin,with the greatest effect observed in children<1 year of age.In addition,observational data suggest that infections such as skin and soft tissue infections,rheumatic heart disease,acute respiratory illness,diarrheal illness,and malaria may all be treated by azithromycin and thus incidentally impacted by MDA-azithromycin.However,the mechanism by which MDA-azithromycin reduces childhood mortality remains unclear.Verbal autopsies performed in MDA-azithromycin childhood mortality studies have produced conflicting data and are underpowered to answer this question.In addition to benefits,there are several important risks associated with MDA-azithromycin.Direct adverse effects potentially resulting from MDA-azithromycin include gastrointestinal side effects,idiopathic hypertrophic pyloric stenosis,cardiovascular side effects,and increase in chronic diseases such as asthma and obesity.Antibacterial resistance is also a risk associated with MDA-azithromycin and has been reported for both gram-positive and enteric organisms.Further,there is the risk for cross-resistance with other antibacterial agents,especially clindamycin.Conclusions: Evidence shows that MDA-azithromycin programs may be beneficial for reducing trachoma,yaws,and mortality in children<5 years of age in certain under-resourced settings.However,there are significant potential risks that need to be considered when deciding how,when,and where to implement these programs.Robust systems to monitor benefits as well as adverse effects and antibacterial resistance are warranted in communities where MDA-azithromycin programs are implemented.
基金financially supported by the National Natural Science Foundation of China(No.52203209)the State Key Laboratory of Solid Waste Reuse for Building Materials,China(No.SWR-2022-009)the Fundamental Research Funds for the Central Universities,China(No.FRF-IDRY22-012)。
文摘Silver nanoparticles(Ag NPs)have attracted attention in the field of biomaterials due to their excellent antibacterial property.However,the reducing and stabilizing agents used for the chemical reduction of Ag NPs are usually toxic and may cause water pollution.In this work,Ag NPs(31.2 nm in diameter)were prepared using the extract of straw,an agricultural waste,as the reducing and stabilizing agent.Experimental analysis revealed that the straw extract contained lignin,the structure of which possesses phenolic hydroxyl and methoxy groups that facilitate the reduction of silver salts into Ag NPs.The surfaces of Ag NPs were negatively charged due to the encapsulation of a thin layer of lignin molecules that prevented their aggregation.After the prepared Ag NPs were added to the precursor solution of acrylamide,free radical polymerization was triggered without the need for extra heating or light irradiation,resulting in the rapid formation of an Ag NP-polyacrylamide composite hydrogel.The inhibition zone test proved that the composite hydrogel possessed excellent antibacterial ability due to the presence of Ag NPs.The prepared hydrogel may have potential applications in the fabrication of biomedical materials,such as antibacterial dressings.
基金supported by the National Natural Science Foundation Joint Fund(Nos.U1806223 and U2106226)the National Natural Science Foundation of China(No.52371081)the Key Technology Research and Development Program of Shandong Province(No.2020CXGC010703).
文摘The defect regulation and p-n heterojunction of composites have gained significant attention due to their potential applications.Nitrogen(N)as doping heteroatoms and perylene-3,4,9,10-tetracarboximide(PDINH)as an appropriate n-type semiconductor were innovatively and reasonably selected to enhance the photocatalytic performance of pristine p-type cuprous oxide(Cu_(2)O).In this study,the defect regula-tion of N doping(1)achieved the small-size effect of Cu_(2)O,(2)optimized the electron features,and(3)improved the kinetics of reactive oxygen species.The p-n heterojunction with PDINH was developed to sharply improve the light utilization of Cu_(2)O,from the UV region to the near-infrared region.As expected,the optimized Cu_(2)N_(x)O_(1–x)/PDINH(x=0.02)exhibited excellent long-term photocatalytic antibacterial ac-tivities,with antibacterial rates exceeding 91%against Staphylococcus aureus and Pseudomonas aeruginosa.Defect regulation and p-n heterojunction of Cu_(2)O-based composites thus provide a great deal of potential for future advancements in photocatalysis.
基金supported by the National Natural Science Foundation of China(No.52001034)the China Postdoctoral Science Foundation(No.2023M731677)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX23_3032).
文摘Nano-zinc oxides(ZnO)demonstrate remarkable antibacterial properties.To further enhance the corrosion resistance and antibacterial efficiency of magnesium alloy micro-arc oxidation(MAO)coatings,this study investigates the preparation of ZnO-containing micro-arc oxidation coatings with dual functionality by incorporating nano-ZnO into MAO electrolyte.The influence of varying ZnO concentrations on the microstructure,corrosion resistance,and antibacterial properties of the coating was examined through microstructure analysis,immersion tests,electrochemical experiments,and antibacterial assays.The findings revealed that the addition of nano-ZnO significantly enhanced the corrosion resistance of the MAO-coated alloy.Specifically,when the ZnO concentration in the electrolyte was 5 g/L,the corrosion rate was more than ten times lower compared to the MAO coatings without ZnO.Moreover,the antibacterial efficacy of ZnO+MAO coating,prepared with a ZnO concentration of 5 g/L,surpassed 95%after 24 h of co-culturing with Staphylococcus aureus(S.aureus).The nano-ZnO+MAO-coated alloy exhibited exceptional degradation resistance,corrosion resistance,and antibacterial effectiveness.
基金supported by Key Scientific and Technological Project of Henan Province(No.242102231060)Doctoral Scientific Research Foundation of Zhoukou Normal University(No.ZKNUC2021041)the Program of Innovative Research Team(in Science and Technology)in University of Henan Province(No.23IRTSTHN008)。
文摘Bacterial infection,insufficient angiogenesis,and oxidative damage are generally regarded as key issues that impede wound healing,making it necessary to prepare new biomaterials to simultaneously address these problems.In this work,monodispersed CeO_(2)@CuS nanocomposites(NCs)were successfully prepared with tannin(TA)as the reductant and linker.Due to abundant oxygen vacancies in CeO_(2)and the polyphenolic structure of TA,the TA-CeO_(2)@CuS NCs exhibited a remarkable antioxidant ability to scavenge excessive reactive oxygen species(ROS),which would likely induce serious inflammation.In addition,the TA-CeO_(2)@CuS NCs demonstrated excellent antibacterial capability with near-infrared ray(NIR)irradiation,and the released copper ions could promote the regeneration of blood vessels.These synergistic effects indicated that the synthesized TA-CeO_(2)@CuS NCs could serve as a promising biomaterial for multimodal wound therapy.
基金supported by the Fundamental Research Funds for the Central Public Welfare Research Institutes,China(Grant Nos.:ZZ16-YQ-037,JIPY2023003,and JJPY2022022)China Academy of Chinese Medical Sciences(CACMS)Innovation Fund(Grant No.:CI2021A00601).
文摘In clinical practice,antibiotics have historically been utilized for the treatment of pathogenic bacteria.However,the gradual emergence of antibiotic resistance among bacterial strains has posed a significant challenge to this approach.In 2022,Escherichia coli,a Gram-negative bacterium renowned for its widespread pathogenicity and high virulence,emerged as the predominant pathogenic bacterium in China.The rapid emergence of antibiotic-resistant E.coli strains has rendered antibiotics insufficient to fight E.coli infections.Traditional Chinese medicine(TCM)has made remarkable contributions to the health of Chinese people for thousands of years,and its significant therapeutic effects have been proven in clinical practice.In this paper,we provide a comprehensive review of the advances and mechanisms of TCM and its active ingredients against antibiotic-resistant E.coli infections.First of all,this review introduces the classification,antibiotic resistance characteristics and mechanisms of E.coli.Then,the TCM formulas and extracts are listed along with their active ingredients against E.coli,including extraction solution,minimum inhibitory concentration(MIC),and the antibacterial mechanisms.In addition,there is growing evidence supporting the synergistic therapeutic strategy of combining TCM with antibiotics for the treatment of antibiotic-resistant E.coli infections,and we provide a summary of this evidence and its underlying mechanisms.In conclusion,we present a comprehensive review of TCM and highlight its potential and advantages in the prevention and treatment of E.coli infections.We hold the opinion that TCM will play an important role in global health,pharmaceutical development,and livestock farming in the future.
基金the National Natural Science Foundation of China(Nos.U2106226,52105297)the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(No.52021003)the Science and Technology Development Project of Jilin Province(Nos.20210203022SF,20210508029RQ).
文摘Superhydrophobic surface is a promising strategy for antibacterial and corrosion protection.However,the use of harmful fluorine-containing materials,poor mechano-chemical stability,the addition of fungicides and poor corrosion resistance often limit its practical application.In this paper,a high-robustness pho-tothermal self-healing superhydrophobic coating is prepared by simply spraying a mixture of hydropho-bically modified epoxy resin and two kinds of modified nanofillers(carbon nanotubes and SiO2)for long-term anticorrosion and antibacterial applications.Multi-scale network and lubrication structures formed by cross-linking of modified carbon nanotubes and repeatable roughness endow coating with high ro-bustness,so that the coating maintains superhydrophobicity even after 100 Taber abrasion cycles,20 m sandpaper abrasion and 100 tape peeling cycles.The synergistic effect of antibacterial adhesion and pho-tothermal bactericidal activity endows coating with excellent antibacterial efficiency,which against Es-cherichia coli(E.coli)and Staphylococcus aureus(S.aureus)separately reaches 99.6% and 99.8%.Moreover,the influence of modified epoxy resin,superhydrophobicity,organic coating and coating thicknesses on the anticorrosion of magnesium(Mg)alloy is systematically studied and analyzed.More importantly,the prepared coating still exhibits excellent self-cleaning,anticorrosion and antibacterial abilities after 20 m abrasion.Furthermore,the coating exhibits excellent adhesion(level 4B),chemical stability,UV radiation resistance,high-low temperature alternation resistance,stable heat production capacity and photother-mal self-healing ability.All these excellent performances can promote its application in a wider range of fields.
基金supported by the National Key Research and Development Program of China(No.2022YFB3808800)the National Natural Science Foundation of China(No.52425112 and 52401178)+1 种基金the IMR Innovation Fund(No.2024-PY06)the CAS-WEGO Research and Development Plan Project.
文摘Titanium alloys,usually known as non-corrodible material,are susceptible to microbiologically influenced corrosion(MIC)in marine environment.While titanium-zirconium(TiZr)alloys have been extensively studied in medical applications,the influence of microorganisms,especially marine microorganisms,on their corrosion behavior has not been explored.In this work,a TiZrCu alloy with a combination of excel-lent mechanical,anti-corrosion,and antibacterial properties was developed by optimizing the Cu content and grain refinement.Its MIC and antibacterial mechanisms against Pseudomonas aeruginosa,a represen-tative marine microorganism,were systematically investigated.5.5 wt%was determined as the optimal copper content.The fine-grained Ti-15Zr-5.5Cu(TZC-5.5FG)alloy maintained high MIC resistance,exhibit-ing a corrosion current of 5.7±0.1 nA/cm^(2) and an antibacterial rate of 91.8% against P.aeruginosa.The mechanism of improved corrosion resistance was attributed to the denser passive film with high TiO2 content and the lower surface potential differenceΔE.The release of Cu^(2+)ions,ΔE,and the generation of ROS are three major factors that contribute to the antibacterial performance of TiZrCu alloys.Com-pared to other available marine metals,TZC-5.5FG alloy exhibited superior comprehensive performance,including excellent mechanical properties and anti-MIC capacity,which make it a promising material for load-bearing applications in marine environment.
文摘Concerns about air quality in dental clinics where aerosol generation during procedures poses significant health risks,have prompted investigations on advanced disinfection technologies.This editorial describes the strengths and limitations of ventilation and aerosol control measures in dental offices,especially with respect to the use of graphene nanocomposites.The potential of graphene nanocomposites as an innovative solution to aerosol-associated health risks is examined in this review due to the unique properties of graphene(e.g.,high con-ductivity,mechanical strength,and antimicrobial activity).These properties have produced promising results in various fields,but the application of graphene in dentistry remains unexplored.The recent study by Ju et al which was published in World Journal of Clinical Cases evaluated the effectiveness of graphene-based air disinfection systems in dental clinics.The study demonstrated that graphene-based disinfection techniques produced significant reductions in suspended particulate matter and bacterial colony counts,when co-mpared with traditional methods.Despite these positive results,challenges such as material saturation,frequency of filter replacement,and associated costs must be addressed before widespread adoption of graphene-based disinfection techniques in clinical practice.Therefore,there is need for further research on material structure optimization,long-term safety evaluations,and broader clinical applications,in order to maximize their positive impact on public health.
基金supported in part by the National Natural Science Foundation of China(12174366)Fundamental Re-search Funds for the Central Universities(WK3450000005)the Anhui Provincial Natural Science Foundation(2108085MC93).
文摘The combination of solar disinfection and photocatalysis technology presents a viable solution for eliminating harmful pathogenic microorganisms from water.However,some photocatalysts(e.g.,zinc oxide-based composites)are susceptible to pH-dependent dissolution in water,which can result in the loss of photocatalysts and additional environ-mental pollution.To obtain zinc oxide-based composites with low dissolution and high antibacterial efficiency for pho-tocatalytic water disinfection,we prepared MoS_(2)/ZnO@CS composites via a precipitation method to encapsulate chitosan(CS)around MoS_(2)/ZnO.The amino groups in the CS molecules act as storerooms for hydrogen ions,which inhibits the dissolution of zinc oxide.In addition,the MoS_(2)/ZnO@CS composites exhibit high production of reactive oxygen species(ROS)and broad-spectrum antibacterial activity under simulated solar irradiation(0.1 W·cm^(-2)).This makes it an excellent antibacterial agent for solar disinfection in water treatment.
文摘A novel 3D metal-organic framework(MOF)[Pr_(2)(L)_(3)(H_(2)O)5·H_(2)O]n(Pr-1),(H_(2)L=4,4'-oxybis(benzoic acid))with a rare structure of broken layer net,was constructed under the condition of solvothermal synthesis.The struc-ture and crystal net were analyzed and characterized.This rod net of Pr-1 is new to both RCSR and ToposPro data-bases,and is named as rn-12 as suggested.Due to the luminescent properties of H_(2)L and Pr(Ⅲ),the solid-state fluo-rescence property and sensing performance(solvents and metal ions)of Pr-1 were investigated.The sensing experi-ments indicated that Pr-1 could act as a fluorescence sensor to detect Cd^(2+)ions with good sensitivity.In addition,antibacterial activities show that Pr-1 exhibited stronger antibacterial activity against Escherichia coli(E.coli),Staphylococcus aureus(S.aureus),and Bacillus subtilis(B.subtilis)compared to synthetic materials.
文摘In order to study the effects of Saussurea laniceps polysaccharides(SLPs)on the expression of inflammatory factors and antimicrobial peptide LL-37 in UVB-induced keratinocytes,SLPs were extracted by the ethanol thermal reflux method,and SLPs at different concentrations were used to examine the inhibitory effect of COX-2(a key mediator of inflammatory pathway).A cell model of UVB irradiation-induced inflammation was established to determine the influence of SLPs on prostaglandin E2(PGE-2),TNF-αand IL-1βinflammatory factors,as well as the relationships of SLPs with LL-37 expression.An enzyme-linked immunosorbent assay(ELISA)and western blot analysis were used to detect the production of inflammatory factors and LL-37 antimicrobial peptide.The results showed that the inhibition rate of COX-2 was 82.41%at 1000μg/mL,and the expression of PGE-2,TNF-αand IL-1βinflammatory factors in HaCaT cells was significantly downregulated at 100μg/mL(P<0.01).In addition,SLPs at 50μg/mL and 100μg/mL concentrations enhanced the expression of LL-37 antimicrobial peptide(P<0.01),thereby down-regulating the expression of TNF-αand IL-1βinflammatory factors,then reducing skin inflammation.Conclusion:SLP can significantly inhibit the inflammatory response induced by UVB,and can further slow down the damage caused by inflammation to the skin by regulating LL-37 antimicrobial peptides,which has the potential to prevent skin inflammatory damage caused by UVB irradiation.
基金National Natural Science Foundation of China(52171114)。
文摘Infections associated with titanium(Ti)-based implants present significant challenges in clinical treatments,especially when biofilms already form on the implant surface.Many antimicrobial agents,including antibiotics,metallic nanoparticles and antimicrobial peptides,have been extensively used to deal with Ti implant infections.However,these chemical approaches suffer from potential toxicity,antibiotic resistance and poor long-term antibacterial performance.Hence,physical antibacterial surfaces on Ti-based implants have attracted increasing attention.The antibacterial behavior of different surfaces on Ti-based biomaterials against various bacteria only by physical properties of the implants themselves(e.g.,nanotopography)or exogenous physical stimulus(e.g.,photocatalysis)was reviewed,as well as parameters influencing the physical antibacterial processes,such as size,shape and density of the surface nanotextures,and bacterial growth phases.Besides,mechanisms of different fabrication techniques for the physical antibacterial surfaces on Ti-based biomaterials were also summarized.
文摘Two Gd_(2)complexes,namely[Gd_(2)(dbm)_(2)(HL_(1))_(2)(CH_(3)OH)_(2)]·4CH_(3)OH(1)and[Gd_(2)(dbm)_(2)(L_(2))_(2)(CH_(3)OH)_(2)]·2CH_(3)OH(2),where H_(3)L_(1)=(Z)-N'-[4-(diethylamino)-2-hydroxybenzylidene]-2-hydroxyacetohydrazide,H_(2)L_(2)=(E)-N'-(5-bromo-2-hydroxy-3-methoxybenzylidene)nicotinohydrazide,Hdbm=dibenzoylmethane,have been constructed by adopting the solvothermal method.Structural characterization unveils that both complexes 1 and 2 are constituted by two Gd^(3+)ions,two dbm-ions,two CH_(3)OH molecules,and two polydentate Schiff-base ligands(HL_(1)^(2-)or L_(2)^(2-)).In addition,complex 1 contains four free methanol molecules,whereas complex 2 harbors two free methanol molecules.By investigating the interactions between complexes 1 and 2 and four types of bacteria(Bacillus subtilis,Escherichia coli,Staphylococcus aureus,Candida albicans),it was found that both complexes 1 and 2 exhibited potent antibacte-rial activities.The interaction mechanisms between the ligands H_(3)L_(1),H_(2)L_(2),complexes 1 and 2,and calf thymus DNA(CT-DNA)were studied using ultraviolet-visible spectroscopy,fluorescence titration,and cyclic voltammetry.The results demonstrated that both complexes 1 and 2 can intercalate into CT-DNA molecules,thereby inhibiting bacterial proliferation to achieve the antibacterial effects.CCDC:2401116,1;2401117,2.
基金supported by One Joint Research Scheme Program 2023(Project ST039-2023).
文摘As a rich source of bioactive compounds,plant-derived natural products hold immense promise for pharmaceutical research and development,offering a path to discovering new drug targets and innovative treatments.Sonchus species,commonly known as sow thistles,are widely distributed across various geographical regions.Several common species within this plant group have a long history of use in traditional medicine,which can be attributed to their diverse therapeutic benefits.Recently,these plants have gained attention for their potential in antimicrobial therapy,owing to their rich phytochemical profile,including sesquiterpene,flavonoids,and phenolic acids with known antimicrobial activities.Currently,available pharmacological studies demonstrate that Sonchus species extracts possess antimicrobial properties,highlighting their potential as sources for new therapeutic agents.This review provides an overview of the current knowledge on the antibacterial,antifungal,antiparasitic,and antiviral activities of the Sonchus species,along with its possible mechanisms of action.The review aims to provide directions for future research and promote the exploration of these plants as viable sources of natural antimicrobial agents,potentially contributing to the development of alternative therapeutic strategies in the face of rising antibiotic resistance.
基金funded by Researchers Supporting Project number(RSP2025R119),King Saud University,Riyadh,Saudi Arabia.
文摘This work uses GC-MS to analyze the bioactive compounds of Salvia rosmarinus essential oils(SREO)and evaluates their antibacterial,antifungal,and insecticidal effects,as well as the major component,1,8-cineole.Chemical analysis identified 16 compounds accounting for 99.19%of the oil’s total content,with 1,8-cineole(33.17%),camphor(16.53%),α-pinene(14.46%),and camphene(8.14%)as the major constituents.Antimicrobial activities were assessed against pathogenic strains using minimal inhibit concentration(MIC)and minimum bactericidal concentration(MBC)assays.SREO exhibited a minimum MIC of 0.128%against P.aeruginosa,while 1,8-cineole showed a minimum MIC of 2.06%against the same strain,highlighting the higher efficacy of the complete oil compared to the isolated compound.Conversely,for antifungal activity,1,8-cineole displayed a lower MIC(2.06%)against A.niger and P.digitatum compared to SREO(4.125%against A.niger).Regarding aphicidal activity,results demonstrated the lethal effects of SREO on M.persicae,with an even more pronounced impact observed for 1,8-cineole.At one dose of 40μL/L air,SREO and 1,8-cineole resulted in 100%insect mortality within 24 h of exposure.After 12 h of exposure to SREO at concentrations of 5,10,20,and 40μL/L air,the mortality rates were 20%,36.67%,70%,and 93.33%.1,8-cineole showed maximum efficacy,achieving complete(100%)mortality within 12 h at 40μL/L air.
基金Financial support from ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No.CZ.02.1.01/0.0/0.0/16_025/0007314)the assistance provided by the Research Infrastructure Nano Envi Cz, supported by the Ministry of Education, Youth and Sports of the Czech Republic under Project No. LM2018124+4 种基金Czech Nano Lab Research Infrastructure supported by MEYS CR (LM2023051)Grant Agency of Gregor Johann Mendel (C-MNG-23–002)further supported by the Ministry of Agriculture of the Czech Republic by Grant RO0523Internal Grant Agency of University of Veterinary Sciences Brno (223/2024/FVHE)the National Institute of Virology and Bacteriology project (Programme EXCELES, Project ID No. LX22NPO5103)-Funded by the European Union-Next Generation EU.
文摘Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses.Therefore,it is impor-tant to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains.In this study,novel selenium-tellurium based nanoparticles(SeTeNPs)were synthesized and characterized.Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model.A total of 10 heifers were divided into experimental and control groups(5 animals each).After intramammary infection with methicillin resistant S.aureus(MRSA)and the development of clinical signs of mastitis,a dose of SeTeNPs was administered to all quarters in the experimental group.Results Based on in vitro tests,the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te,respectively,was used for application into the mammary gland.Three days after SeTeNPs administration,MRSA counts in the experimental group showed a significant reduction(P<0.01)compared to the control group.The inhibitory effect observed within the in vitro experiments was thus confirmed,resulting in the suppression of infection in ani-mals.Moreover,the superior biocompatibility of SeTeNPs in the organism was demonstrated,as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application,i.e.,mammary gland,compared to the control group(P>0.05).Additionally,the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected,indicating that the nanoparticles had no adverse effects on the organism.Conclusions Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the pres-ence of resistant bacteria.However,the current study is limited by its small sample size,making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs.Therefore,further research with a larger sample size is needed to validate these results.
基金Prince Sattam bin Abdulaziz University for funding this research work through the project number(No.PSAU/2023/01/27406).
文摘This study aims to optimize biosurfactant production by Streptomyces gobitricini strain BS-2.After the initial screening,the effective factors on the production of biosurfactant were investigated with the two-level factorial statistical.The physical and chemical features and structure of the biosurfactant crafted were analyzed using FTIR,CHNS,EDX,and SEM.The extracted biosurfactant resulted in a decrease in interfacial tension from 72 mN/m to 29 mN/m,with a critical micelle concentration(CMC)of 300 mg/mL.The optimum medium for biosurfactant production that led to utmost diminution in culture broth surface tension(29 mN/m)was:crude oil(1.5%,v/v),hydrolyzed protein(1 g/L),yeast extract(0.5 g/L),molasses(1.5 g/L)and olive oil(1%v/v).The glycolipid organization of the microbial surfactant was confirmed via FTIR,CHNS,EDX,and SEM analysis.Analysis using GC indicated that the presence of biosurfactant increased crude oil degradation by S.gobitricini strain BS-2 dramatically(87%)and this bacterium effectively degraded most of its alkane components.Furthermore,the glycolipid biosurfactant displayed significant development repression against therapeutic bacterial microbes.In summary,the glycolipid biosurfactant produced by S.gobitricini strain BS-2 exhibited diverse functional properties and showed promise for potential biomedical and biotechnological applications.
