Objective:To evaluate the antiarthritic activity of water extract of leaves of Vernonia amygdalina(V.A.) on arthritis induced model of rats.Methods:Arthritis was induced in male albino Wister rats by injection of crot...Objective:To evaluate the antiarthritic activity of water extract of leaves of Vernonia amygdalina(V.A.) on arthritis induced model of rats.Methods:Arthritis was induced in male albino Wister rats by injection of croton oil(0.1 mL) into the left foot pad of the animals.Treatment with V.A.at 200 and 400 mg/kg and standard Indomethacin(0.3 mg/kg) was started on the same day and continued up to the 12th day.The paw volume was measured on the 1st,5th,12th and 21st day,respectively for both the paws and anti-arthritic activity was evaluated.Results:The extract of V.A.produced reduction in the inflammation of the paw due to croton oil.The antiarthritic action started on the 5th day and continued till the 12th day and the activity was comparable to that of the standard on both days.V.A.significantly inhibited adjuvant induced arthritis and had significant ant-inflammatory effect(P【0.05).Conclusion:This report therefore clearly showed that V.A.significantly inhibited adjuvant induced arthritis in rats as it significantly reduced the paw volume on the 12th day and may explain the effectiveness of this plant when used in the tropics for the treatment of arthritis.展开更多
The present study was aimed to investigate the anti-arthritic activity of alcoholic extract ofClerodendron inerme which was shown to inhibit inflammatory process. Arthritis was induced in rats by injecting rats intrad...The present study was aimed to investigate the anti-arthritic activity of alcoholic extract ofClerodendron inerme which was shown to inhibit inflammatory process. Arthritis was induced in rats by injecting rats intradermally with Freund＇s adjuvant 0.01%-0.1% in paraffin oil. They were grouped and received 10 mg/100 gm b.wt. of alcoholic fraction of C. inerme, 1.5 mg/100 gm b.wt. hydrocortisone and saline, orally, for 15 days. The arthritic scores, radiological scores, body weights, liver glycogen, serum mucoproteins were monitored for 49 days. Wilcoxan ranking methods and Student＇s t Test were used to determine the significance changes between control and treated groups. Adjuvant-arthritic rats showed a significant decrease in body weight, liver glycogen, increased serum mucoprotein and severity of periostitis and other degenerative changes m the bone. But treatment with the alcoholic fraction （apigenin, scutallarin and pectinolinergenin） of C. inerme exhibited a very good relief from the arthritic conditions by increasing the body weight, increased liver glycogen content and registered little degenerative changes in bones, suggesting long term anti-arthritic activity when compared to the control group. The anti-arthritic activity ofC. inetme alcoholic fraction was remarkable in this study. However, further studies are required to elucidate its full anti-arthritic potential.展开更多
Objective: To extract and isolate berberine from Berberis aristata(Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was analyzed.Methods: The berbe...Objective: To extract and isolate berberine from Berberis aristata(Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was analyzed.Methods: The berberine was isolated from B. aristata using microwave-assisted extraction(MAE) and characterised by a spectroscopic technique. The isolated berberine was evaluated for its antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays, while antiarthritic activity was evaluated in the complete freund’s adjuvant(CFA)-induced arthritis rat model.Results: The antioxidant activity of berberine revealed potent antioxidant activity in DPPH, nitric oxide,and superoxide scavenging assays. The in vivo antiarthritic activity of berberine in the CFA-induced arthritis rat model showed a significant reduction in paw diameter, arthritic score, and an increase in body weight. Furthermore, a concentration-dependent ameliorating action of berberine on haematological parameters was noticed. Proinfammatory biomarkers, including IL-6, IL-10, and TGF-b in serum were reported, and histopathology examination revealed that berberine decreased pannus formation, synovial hyperplasia, and bone erosion. Radiographic investigation showed soft tissue infammation, bone resorption and erosion, joint gap reduction, and substantial connective tissue expansion after treatment with berberine.Conclusion: The ameliorating action on haematological parameters and proinfammatory biomarkers of berberine makes them a suitable remedy for the treatment of arthritis.展开更多
Background:Chaetomorpha aerea,a marine green alga,has drawn attention because of its rich phytochemical constituents and therapeutic benefits.Using an integrated approach that combined in vitro,in vivo,and in silico a...Background:Chaetomorpha aerea,a marine green alga,has drawn attention because of its rich phytochemical constituents and therapeutic benefits.Using an integrated approach that combined in vitro,in vivo,and in silico approaches,this work examined the antioxidant,anti-inflammatory,and antidiabetic qualities of acetone extract of C.aerea(AECA).Methods:Total phenolic and flavonoid concentrations of AECA were measured.Antioxidant activity was assessed using the DPPH and ABTS free radical scavenging assays.In vitro protein denaturation and in vivo carrageenan-induced paw edema models were employed to evaluate the anti-inflammatory potential,whereas antidiabetic activity was assessed using in vitroα-amylase inhibition and in vivo oral glucose tolerance test(OGTT).Molecular docking and ADME/T analysis were employed to further analyze bioactive compounds identified using gas chromatography–mass spectrometry(GC–MS).Result:Antioxidant activity demonstrated a minimum inhibitory concentration(IC_(50))of 107.44μg/mL for DPPH and 118.23μg/mL for ABTS.In vitro anti-inflammatory assays indicated a suppression of protein denaturation at a concentration of 102μg/mL(IC_(50)),where AECA(400 mg/kg)resulted in a 27%reduction in paw edema at 6 h in the mouse model.In vitro antidiabetic test indicatedα-amylase inhibition with an IC_(50) value of 70.72μg/mL,and in the OGTT,a significant lowering of blood glucose was recorded at 120 min in mice.Strong binding affinities were observed for stigmasta-5,24(28)-dien-3-ol,identified using GC–MS,with values of−9.9 kcal/mol forα-amylase and−8.0 kcal/mol for cyclooxygenase-2.Conclusion:C.aerea serves as an effective natural remedy for oxidative stress,inflammation,and hyperglycemia.These findings advocate for further clinical and mechanistic investigations to optimize therapeutic efficacy.展开更多
Objective:To evaluate the efficacy of boswellic acid against monosodium urate crystal-induced inflammation in mice.Methods:The mice were divided into four experimental groups.GroupⅠserved as control;mice in groupⅡwe...Objective:To evaluate the efficacy of boswellic acid against monosodium urate crystal-induced inflammation in mice.Methods:The mice were divided into four experimental groups.GroupⅠserved as control;mice in groupⅡwere injected with monosodium urate crystal;groupⅢconsisted of monosodium urate crystal-induced mice who were treated with boswellic acid(30mg/kg/b.w.);groupⅣcomprised monosodium urate crystal-induced mice who were treated with indomethacin(3mg/kg/b.w.).Paw volume and levels/activities of lysosomal enzymes,lipid peroxidation,anti-oxidant status and inflammatory mediator TNF-αwere determined in control and monosodium urate crystal-induced mice.In addition,the levels ofβ-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes(PMNL)in vitro.Results:The activities of lysosomal enzymes,lipid peroxidation,and tumour necrosis factor-αlevels and paw volume were increased significantly in monosodium urate crystal-induced mice,whereas the activities of antioxidant status were in turn decreased.However,these changes were modulated to near normal levels upon boswellic acid administration.In vitro,boswellic acid reduced the level ofβ-glucuronidase and lactate dehydrogenase in monosodium urate crystal-incubated PMNL in concentration dependent manner when compared with control cells.Conclusions:The results obtained in this study further strengthen the anti-inflammatory/antiarthritic effect of boswellic acid,which was already well established by several investigators.展开更多
文摘Objective:To evaluate the antiarthritic activity of water extract of leaves of Vernonia amygdalina(V.A.) on arthritis induced model of rats.Methods:Arthritis was induced in male albino Wister rats by injection of croton oil(0.1 mL) into the left foot pad of the animals.Treatment with V.A.at 200 and 400 mg/kg and standard Indomethacin(0.3 mg/kg) was started on the same day and continued up to the 12th day.The paw volume was measured on the 1st,5th,12th and 21st day,respectively for both the paws and anti-arthritic activity was evaluated.Results:The extract of V.A.produced reduction in the inflammation of the paw due to croton oil.The antiarthritic action started on the 5th day and continued till the 12th day and the activity was comparable to that of the standard on both days.V.A.significantly inhibited adjuvant induced arthritis and had significant ant-inflammatory effect(P【0.05).Conclusion:This report therefore clearly showed that V.A.significantly inhibited adjuvant induced arthritis in rats as it significantly reduced the paw volume on the 12th day and may explain the effectiveness of this plant when used in the tropics for the treatment of arthritis.
文摘The present study was aimed to investigate the anti-arthritic activity of alcoholic extract ofClerodendron inerme which was shown to inhibit inflammatory process. Arthritis was induced in rats by injecting rats intradermally with Freund＇s adjuvant 0.01%-0.1% in paraffin oil. They were grouped and received 10 mg/100 gm b.wt. of alcoholic fraction of C. inerme, 1.5 mg/100 gm b.wt. hydrocortisone and saline, orally, for 15 days. The arthritic scores, radiological scores, body weights, liver glycogen, serum mucoproteins were monitored for 49 days. Wilcoxan ranking methods and Student＇s t Test were used to determine the significance changes between control and treated groups. Adjuvant-arthritic rats showed a significant decrease in body weight, liver glycogen, increased serum mucoprotein and severity of periostitis and other degenerative changes m the bone. But treatment with the alcoholic fraction （apigenin, scutallarin and pectinolinergenin） of C. inerme exhibited a very good relief from the arthritic conditions by increasing the body weight, increased liver glycogen content and registered little degenerative changes in bones, suggesting long term anti-arthritic activity when compared to the control group. The anti-arthritic activity ofC. inetme alcoholic fraction was remarkable in this study. However, further studies are required to elucidate its full anti-arthritic potential.
文摘Objective: To extract and isolate berberine from Berberis aristata(Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was analyzed.Methods: The berberine was isolated from B. aristata using microwave-assisted extraction(MAE) and characterised by a spectroscopic technique. The isolated berberine was evaluated for its antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays, while antiarthritic activity was evaluated in the complete freund’s adjuvant(CFA)-induced arthritis rat model.Results: The antioxidant activity of berberine revealed potent antioxidant activity in DPPH, nitric oxide,and superoxide scavenging assays. The in vivo antiarthritic activity of berberine in the CFA-induced arthritis rat model showed a significant reduction in paw diameter, arthritic score, and an increase in body weight. Furthermore, a concentration-dependent ameliorating action of berberine on haematological parameters was noticed. Proinfammatory biomarkers, including IL-6, IL-10, and TGF-b in serum were reported, and histopathology examination revealed that berberine decreased pannus formation, synovial hyperplasia, and bone erosion. Radiographic investigation showed soft tissue infammation, bone resorption and erosion, joint gap reduction, and substantial connective tissue expansion after treatment with berberine.Conclusion: The ameliorating action on haematological parameters and proinfammatory biomarkers of berberine makes them a suitable remedy for the treatment of arthritis.
文摘Background:Chaetomorpha aerea,a marine green alga,has drawn attention because of its rich phytochemical constituents and therapeutic benefits.Using an integrated approach that combined in vitro,in vivo,and in silico approaches,this work examined the antioxidant,anti-inflammatory,and antidiabetic qualities of acetone extract of C.aerea(AECA).Methods:Total phenolic and flavonoid concentrations of AECA were measured.Antioxidant activity was assessed using the DPPH and ABTS free radical scavenging assays.In vitro protein denaturation and in vivo carrageenan-induced paw edema models were employed to evaluate the anti-inflammatory potential,whereas antidiabetic activity was assessed using in vitroα-amylase inhibition and in vivo oral glucose tolerance test(OGTT).Molecular docking and ADME/T analysis were employed to further analyze bioactive compounds identified using gas chromatography–mass spectrometry(GC–MS).Result:Antioxidant activity demonstrated a minimum inhibitory concentration(IC_(50))of 107.44μg/mL for DPPH and 118.23μg/mL for ABTS.In vitro anti-inflammatory assays indicated a suppression of protein denaturation at a concentration of 102μg/mL(IC_(50)),where AECA(400 mg/kg)resulted in a 27%reduction in paw edema at 6 h in the mouse model.In vitro antidiabetic test indicatedα-amylase inhibition with an IC_(50) value of 70.72μg/mL,and in the OGTT,a significant lowering of blood glucose was recorded at 120 min in mice.Strong binding affinities were observed for stigmasta-5,24(28)-dien-3-ol,identified using GC–MS,with values of−9.9 kcal/mol forα-amylase and−8.0 kcal/mol for cyclooxygenase-2.Conclusion:C.aerea serves as an effective natural remedy for oxidative stress,inflammation,and hyperglycemia.These findings advocate for further clinical and mechanistic investigations to optimize therapeutic efficacy.
文摘Objective:To evaluate the efficacy of boswellic acid against monosodium urate crystal-induced inflammation in mice.Methods:The mice were divided into four experimental groups.GroupⅠserved as control;mice in groupⅡwere injected with monosodium urate crystal;groupⅢconsisted of monosodium urate crystal-induced mice who were treated with boswellic acid(30mg/kg/b.w.);groupⅣcomprised monosodium urate crystal-induced mice who were treated with indomethacin(3mg/kg/b.w.).Paw volume and levels/activities of lysosomal enzymes,lipid peroxidation,anti-oxidant status and inflammatory mediator TNF-αwere determined in control and monosodium urate crystal-induced mice.In addition,the levels ofβ-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes(PMNL)in vitro.Results:The activities of lysosomal enzymes,lipid peroxidation,and tumour necrosis factor-αlevels and paw volume were increased significantly in monosodium urate crystal-induced mice,whereas the activities of antioxidant status were in turn decreased.However,these changes were modulated to near normal levels upon boswellic acid administration.In vitro,boswellic acid reduced the level ofβ-glucuronidase and lactate dehydrogenase in monosodium urate crystal-incubated PMNL in concentration dependent manner when compared with control cells.Conclusions:The results obtained in this study further strengthen the anti-inflammatory/antiarthritic effect of boswellic acid,which was already well established by several investigators.
