Objective： To investigate whether dietary daidzein interact with endogenous 17β-Estradiol （E2） to give rise to additive or inhibitory effects on proliferation and apoptosis in breast cancer cells. Methods： Cell ...Objective： To investigate whether dietary daidzein interact with endogenous 17β-Estradiol （E2） to give rise to additive or inhibitory effects on proliferation and apoptosis in breast cancer cells. Methods： Cell cycle distribution and apoptosis induction were analyzed by using flow cytometry when breast cancer cell lines MCF-7 were cotreated with daidzein （1, 5 μmol/L） and E2 （0.1-10 nmol/L） for 5 days. Whether daidzein could alter E2-modulated mRNA expression of estrogen receptor alpha （ERα）, estrogen receptor beta （ERI3） and ERβ-estrogen response element （ERE） dependent transcription was investigated by RT-PCR and luciferase induction assays. The effects of daidzein on E2-modulated expression of proapoptotic p53, bax and antiapoptotic bcl-2 at both mRNA and protein levels were also investigated by RT-PCR and Western blot. Results： Daidzein enhanced the antiapoptotic effect in an Ea dose-dependent manner, but had no effect on E2-induced proliferation. Daidzein antagonized E2-induced ERβ mRNA expression and ERβ-ERE dependent transcription. In addition, daidzein only antagonized E2-upregulated expression of p53 and bax, but had no effect on E2-upregulated expression of bcl-2. Conclusion： Daidzein enhances the antiapoptotic effect of E2 on breast cancer cells by inhibiting E2-mediated p53-bax proapoptotic pathway. These results suggest that dietary daidzein may enhance deleterious effect of endogenous E2 in hormone-dependent breast cancer.展开更多
The polysaccharides from pumpkin fruit （PP） were obtained and purified by hot-water extraction, anion-exchange chromatography, and gel column chromatography. The physicochemical properties of PP were determined by g...The polysaccharides from pumpkin fruit （PP） were obtained and purified by hot-water extraction, anion-exchange chromatography, and gel column chromatography. The physicochemical properties of PP were determined by gel filtration chromatography, gas chromatography, fourier transform infrared （FTIR） spectroscopy, and nuclear magnetic resonance （NMR） spectroscopy. Results indicated that the molecular weight of PP was about 23 kDa and PP was composed of D-Arabinose, D-Marmose, D-Glucose, and D-Galactose with a molar ratio of 1 ： 7.79 ： 70.32 ： 7.05. FTIR and NMR spectra indicated that PP was the polysaccharide containing pyranose ring. Additionally, PP protected islets cells from streptozotocin （STZ） injury in vitro via increasing the levels of super-oxide dismutase （SOD） and malondialdehyde （MDA） and reducing the production of NO. The experiment of reverse transcriptase-polymerase chain reaction further proved that PP inhibited apoptosis via modulating the expression of Bax/Bcl-2 in STZ-damaged islet cells. In conclusion, PP could be explored as a novel agent for the treatment of diabetes mellitus.展开更多
Spinal cord injury（SCI） from trauma or disease severely impairs sensory and motor function. Neurorehabilitation after SCI is a complex medical process that focuses on improving neurologic function and repairing dama...Spinal cord injury（SCI） from trauma or disease severely impairs sensory and motor function. Neurorehabilitation after SCI is a complex medical process that focuses on improving neurologic function and repairing damaged connections in the central nervous system. An increasing number of preclinical studies suggest that melatonin may be useful for the treatment of SCI. Melatonin is an indolamine that is primarily secreted by the pineal gland and known to be regulated by photoperiodicity. However, it is also a versatile hormone with antioxidative, antiapoptotic, neuroprotective, and anti-inflammatory properties. Here, we review the neuroprotective properties of melatonin and the potential mechanisms by which it might be beneficial in the treatment of SCI. We also describe therapies that combine melatonin with exercise, oxytetracycline, and dexamethasone to attenuate the secondary injury after SCI and limit potential side effects. Finally, we discuss how injury at different spinal levels may differentially affect the secretion of melatonin.展开更多
Survivin is an antiapoptotic protein expressed in tumor cells that correlates with aggressiveness, prognosis and treatment. Objective: This review summarizes significance of survivin expression among different tumor t...Survivin is an antiapoptotic protein expressed in tumor cells that correlates with aggressiveness, prognosis and treatment. Objective: This review summarizes significance of survivin expression among different tumor types highlighting the potential value of survivin as an important biomarker that may have an impact on patient’s response to therapy with prognostic implications.展开更多
Giant congenital melanocytic nevus(GCMN)is a RAS/RAF mutation-driven syndrome characterized by extensive melanocytic lesions,posing psychological challenges and a lifelong risk of malignancy.Existing treatments like s...Giant congenital melanocytic nevus(GCMN)is a RAS/RAF mutation-driven syndrome characterized by extensive melanocytic lesions,posing psychological challenges and a lifelong risk of malignancy.Existing treatments like surgical resection and laser therapy fail to fully remove lesions,and MAPK inhibitors show limited efficacy.This study identified a predominant population of senescent cells and a minority of proliferative cells in GCMN,necessitating dual-targeted strategies.We found that the antiapoptotic protein BCL2 is expressed in both senescent and proliferative cells from GCMN patients with various gene mutations.Coexpression of P16 and BCL2 indicated a phenotype of growth arrest and cell survival.BCL2 inhibitors(BCL2i)showed significant cytotoxicity to GCMN cells in vitro.Hypopigmentation and GCMN cell clearance were observed in patient-derived xenograft models and in Nras^(Q61K)-mutated and Braf^(V600)E-mutated transgenic models following BCL2i treatment.Histology of regressed GCMN indicated extensive immune cell infltration,suggesting immune involvement.Single-cell sequencing and immunostaining revealed that activated neutrophils formed extracellular traps,synergizing with BCL2i to treat GCMN.Neutrophil depletion and immunosuppression reduce treatment efficacy,highlighting the crucial role of the immune response post-BCL2i treatment.Longterm follow-up showed no recurrence,with neutrophils and T cells residing in the dermis,indicating memory immune reactions.These findings present a promising therapeutic strategy and underscore the translational potential of BCL2i in treating GCMN.展开更多
Steroids and ecdysteroids have been shown to exhibit a range of biological effects,including anti-inflammatory,anticancer,and neuroprotective.This systematic review aims to highlight the evidence-based neuroprotective...Steroids and ecdysteroids have been shown to exhibit a range of biological effects,including anti-inflammatory,anticancer,and neuroprotective.This systematic review aims to highlight the evidence-based neuroprotective and antioxidant effects of steroids and ecdysteroids in SH-SY5Y neuroblastoma cells.A comprehensive literature search was conducted on May 11,2023,without publication source restrictions,using various electronic databases,including PubMed,Web of Science(WoS),Scopus,and Cumulative Index toNursing and Allied Health Literature.Of 103articles,only20 studies were included for investigating the neuroprotective effects of steroids and ecdysteroids in SH-SY5Y cells exposed to oxidative stress or neurotoxic agents.The risk of bias and quality assessment of the included studies were evaluated in accordance with the Nature Publication Quality Improvement Project specific criteria.The selected studies reported the antioxidant effects of the tested compounds on SH-SY5Y cells and demonstrated their ability to scavenge free radicals and prevent lipid peroxidation.These findings suggest that neurosteroids have potential therapeutic applications for the prevention and treatment of neurodegenerative diseases characterized by oxidative stress and neuronal damage.Some studies have investigated the molecular mechanisms underlying the neuroprotective and antioxidant effects of steroids and ecdysteroids in SH-SY5Y cells.These mechanisms include the activation of antioxidant enzymes,such as superoxide dismutase and glutathione peroxidase,and the modulation of various signaling pathways,including the phosphoinositide 3-kinase/protein kinase B and mitogen-activated protein kinase/extracellular signal-regulated kinase pathways.This review provides evidence that the tested compounds have remarkable neuroprotective and antioxidant effects in human neuroblastoma SH-SY5Y cells.展开更多
基金supported by the National Natural Science Foundation of China (No.30671508)by State Key Laboratory for Agrobiotechnology of China (No.2009SKLAB07-5)
文摘Objective： To investigate whether dietary daidzein interact with endogenous 17β-Estradiol （E2） to give rise to additive or inhibitory effects on proliferation and apoptosis in breast cancer cells. Methods： Cell cycle distribution and apoptosis induction were analyzed by using flow cytometry when breast cancer cell lines MCF-7 were cotreated with daidzein （1, 5 μmol/L） and E2 （0.1-10 nmol/L） for 5 days. Whether daidzein could alter E2-modulated mRNA expression of estrogen receptor alpha （ERα）, estrogen receptor beta （ERI3） and ERβ-estrogen response element （ERE） dependent transcription was investigated by RT-PCR and luciferase induction assays. The effects of daidzein on E2-modulated expression of proapoptotic p53, bax and antiapoptotic bcl-2 at both mRNA and protein levels were also investigated by RT-PCR and Western blot. Results： Daidzein enhanced the antiapoptotic effect in an Ea dose-dependent manner, but had no effect on E2-induced proliferation. Daidzein antagonized E2-induced ERβ mRNA expression and ERβ-ERE dependent transcription. In addition, daidzein only antagonized E2-upregulated expression of p53 and bax, but had no effect on E2-upregulated expression of bcl-2. Conclusion： Daidzein enhances the antiapoptotic effect of E2 on breast cancer cells by inhibiting E2-mediated p53-bax proapoptotic pathway. These results suggest that dietary daidzein may enhance deleterious effect of endogenous E2 in hormone-dependent breast cancer.
基金supported by the Natural Science Foundation Committee of China(NSFC 81202467)the Natural Science Foundation of Jiangsu Province in China(BK2012232)+2 种基金the Natural Science Research Project of Universities in Jiangsu Province of China(11KJB350004,06KJD310150,12KJB310012)the Natural Science Foundation of Nantong City of China(BK2011044)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The polysaccharides from pumpkin fruit （PP） were obtained and purified by hot-water extraction, anion-exchange chromatography, and gel column chromatography. The physicochemical properties of PP were determined by gel filtration chromatography, gas chromatography, fourier transform infrared （FTIR） spectroscopy, and nuclear magnetic resonance （NMR） spectroscopy. Results indicated that the molecular weight of PP was about 23 kDa and PP was composed of D-Arabinose, D-Marmose, D-Glucose, and D-Galactose with a molar ratio of 1 ： 7.79 ： 70.32 ： 7.05. FTIR and NMR spectra indicated that PP was the polysaccharide containing pyranose ring. Additionally, PP protected islets cells from streptozotocin （STZ） injury in vitro via increasing the levels of super-oxide dismutase （SOD） and malondialdehyde （MDA） and reducing the production of NO. The experiment of reverse transcriptase-polymerase chain reaction further proved that PP inhibited apoptosis via modulating the expression of Bax/Bcl-2 in STZ-damaged islet cells. In conclusion, PP could be explored as a novel agent for the treatment of diabetes mellitus.
基金supported by the National Natural Science Foundation of China,No.81671161(to ZJL)
文摘Spinal cord injury（SCI） from trauma or disease severely impairs sensory and motor function. Neurorehabilitation after SCI is a complex medical process that focuses on improving neurologic function and repairing damaged connections in the central nervous system. An increasing number of preclinical studies suggest that melatonin may be useful for the treatment of SCI. Melatonin is an indolamine that is primarily secreted by the pineal gland and known to be regulated by photoperiodicity. However, it is also a versatile hormone with antioxidative, antiapoptotic, neuroprotective, and anti-inflammatory properties. Here, we review the neuroprotective properties of melatonin and the potential mechanisms by which it might be beneficial in the treatment of SCI. We also describe therapies that combine melatonin with exercise, oxytetracycline, and dexamethasone to attenuate the secondary injury after SCI and limit potential side effects. Finally, we discuss how injury at different spinal levels may differentially affect the secretion of melatonin.
文摘Survivin is an antiapoptotic protein expressed in tumor cells that correlates with aggressiveness, prognosis and treatment. Objective: This review summarizes significance of survivin expression among different tumor types highlighting the potential value of survivin as an important biomarker that may have an impact on patient’s response to therapy with prognostic implications.
基金supported by the National Natural Science Foundation of China(Grant No.81871595,82372533)the Shanghai Clinical Research Center of Plastic and Reconstructive Surgery affliated to Science and Technology Commission of Shanghai Municipality(GrantNo.22MC1940300).
文摘Giant congenital melanocytic nevus(GCMN)is a RAS/RAF mutation-driven syndrome characterized by extensive melanocytic lesions,posing psychological challenges and a lifelong risk of malignancy.Existing treatments like surgical resection and laser therapy fail to fully remove lesions,and MAPK inhibitors show limited efficacy.This study identified a predominant population of senescent cells and a minority of proliferative cells in GCMN,necessitating dual-targeted strategies.We found that the antiapoptotic protein BCL2 is expressed in both senescent and proliferative cells from GCMN patients with various gene mutations.Coexpression of P16 and BCL2 indicated a phenotype of growth arrest and cell survival.BCL2 inhibitors(BCL2i)showed significant cytotoxicity to GCMN cells in vitro.Hypopigmentation and GCMN cell clearance were observed in patient-derived xenograft models and in Nras^(Q61K)-mutated and Braf^(V600)E-mutated transgenic models following BCL2i treatment.Histology of regressed GCMN indicated extensive immune cell infltration,suggesting immune involvement.Single-cell sequencing and immunostaining revealed that activated neutrophils formed extracellular traps,synergizing with BCL2i to treat GCMN.Neutrophil depletion and immunosuppression reduce treatment efficacy,highlighting the crucial role of the immune response post-BCL2i treatment.Longterm follow-up showed no recurrence,with neutrophils and T cells residing in the dermis,indicating memory immune reactions.These findings present a promising therapeutic strategy and underscore the translational potential of BCL2i in treating GCMN.
基金Stipendium Hungaricum Scholarship founded by the Hungarian Government。
文摘Steroids and ecdysteroids have been shown to exhibit a range of biological effects,including anti-inflammatory,anticancer,and neuroprotective.This systematic review aims to highlight the evidence-based neuroprotective and antioxidant effects of steroids and ecdysteroids in SH-SY5Y neuroblastoma cells.A comprehensive literature search was conducted on May 11,2023,without publication source restrictions,using various electronic databases,including PubMed,Web of Science(WoS),Scopus,and Cumulative Index toNursing and Allied Health Literature.Of 103articles,only20 studies were included for investigating the neuroprotective effects of steroids and ecdysteroids in SH-SY5Y cells exposed to oxidative stress or neurotoxic agents.The risk of bias and quality assessment of the included studies were evaluated in accordance with the Nature Publication Quality Improvement Project specific criteria.The selected studies reported the antioxidant effects of the tested compounds on SH-SY5Y cells and demonstrated their ability to scavenge free radicals and prevent lipid peroxidation.These findings suggest that neurosteroids have potential therapeutic applications for the prevention and treatment of neurodegenerative diseases characterized by oxidative stress and neuronal damage.Some studies have investigated the molecular mechanisms underlying the neuroprotective and antioxidant effects of steroids and ecdysteroids in SH-SY5Y cells.These mechanisms include the activation of antioxidant enzymes,such as superoxide dismutase and glutathione peroxidase,and the modulation of various signaling pathways,including the phosphoinositide 3-kinase/protein kinase B and mitogen-activated protein kinase/extracellular signal-regulated kinase pathways.This review provides evidence that the tested compounds have remarkable neuroprotective and antioxidant effects in human neuroblastoma SH-SY5Y cells.
