Cyperus esculentus(Tiger nut)has been grossly underutilized and unexploited despite its nutritional and medicinal potentials.This research aimed to evaluate the phytochemical,proximate,GC-MS and pharmacological proper...Cyperus esculentus(Tiger nut)has been grossly underutilized and unexploited despite its nutritional and medicinal potentials.This research aimed to evaluate the phytochemical,proximate,GC-MS and pharmacological properties such as antioxidant,anticancer,anti-elastase,anti-melanogenic and anti-tyrosinase activity in ethanol and n-hexane extracts of tiger nut.The pharmacological activities were measured using spectrophotometry,agar well diffusion,MCF-7 and HeLa cell methods.The tiger nut extracts(ethanol and n-hexane)in the phytochemical and proximate analysis showed the existence of bioactive compounds such as alkaloids,glycosides,flavonoids,crude fibres,tannins,proteins,carbohydrates,oxalates,phytates and fats content.The ethanol extract showed superior pharmacological(anticancer,antibacterial,anti-melanogenic and antioxidant)activities,especially against the MCF-7 cell line.The results for scavenging activity of ethanol extract(IC_(50)=535.57μg/mL)showed significant differences(p<0.05)when compared with n-hexane extract(IC_(50)=645.5μg/mL).The GC-MS assessment of both extracts showed the existence of bioactive compounds.Different concentrations(100,200 and 350 mg/mL)of tiger nut extracts against Shigella sp.,Salmonella sp.,Staphylococcus aureus and Escherichia coli demonstrated the highest level of inhibitions at 350 mg/mL.The findings of the current research showed that tiger nut has a high content of nutrients and bioactive phytochemicals.展开更多
Coconut water(CW)is a popular natural hydration drink in tropical countries.It is rich in essential electrolytes,vitamins,minerals,and bioactive compounds with functional properties.Therefore,this work aimed to evalua...Coconut water(CW)is a popular natural hydration drink in tropical countries.It is rich in essential electrolytes,vitamins,minerals,and bioactive compounds with functional properties.Therefore,this work aimed to evaluate the functional properties(i.e.,antioxidant and anti-aging activities)and to determine selected bioactive compounds in young and mature CW from the four most popular coconut varieties in Malaysia namely,Aromatic Green Dwarf(PDN),Malaysian Yellow Dwarf(MYD),MATAG(MYD×Tagnanan)and MAWA(MYD×West African Tall).Spectrophotometric and liquid chromatography methods were used to quantify the changes in the tested parameters.It is shown that the quantification of selected bioactive compounds and functional activities of CW displayed a mixed pattern at different maturation.Results from antioxidant,anti-aging activities,amino acids profile and bioactive compounds such as gallic acid,catechin,trans-zeatin,and dihydrozeatin were measured to decrease with the coconuts’maturation.Meanwhile,the amount of kinetin,kinetin riboside and trans-zeatin riboside increased with the maturation of the coconuts.Specifically,CW from young MYD showed the highest antioxidant and anti-aging activities and possessed diverse bioactive compounds that could serve as novel sources of natural functional ingredients targeted for skin anti-aging.In addition,the sensory evaluation showed that the taste of young and mature CW is acceptable for consumption with the young PDN,young MYD and mature MYD displaying the highest score.To the best of our knowledge,the present work reported the first attempt to elucidate the inhibitory activities of CW towards elastase and collagenase enzymes.展开更多
基金The authors wish to thank Mr Joseph Sani Sahjury(department of Information Technology)for SPSS training during this research.
文摘Cyperus esculentus(Tiger nut)has been grossly underutilized and unexploited despite its nutritional and medicinal potentials.This research aimed to evaluate the phytochemical,proximate,GC-MS and pharmacological properties such as antioxidant,anticancer,anti-elastase,anti-melanogenic and anti-tyrosinase activity in ethanol and n-hexane extracts of tiger nut.The pharmacological activities were measured using spectrophotometry,agar well diffusion,MCF-7 and HeLa cell methods.The tiger nut extracts(ethanol and n-hexane)in the phytochemical and proximate analysis showed the existence of bioactive compounds such as alkaloids,glycosides,flavonoids,crude fibres,tannins,proteins,carbohydrates,oxalates,phytates and fats content.The ethanol extract showed superior pharmacological(anticancer,antibacterial,anti-melanogenic and antioxidant)activities,especially against the MCF-7 cell line.The results for scavenging activity of ethanol extract(IC_(50)=535.57μg/mL)showed significant differences(p<0.05)when compared with n-hexane extract(IC_(50)=645.5μg/mL).The GC-MS assessment of both extracts showed the existence of bioactive compounds.Different concentrations(100,200 and 350 mg/mL)of tiger nut extracts against Shigella sp.,Salmonella sp.,Staphylococcus aureus and Escherichia coli demonstrated the highest level of inhibitions at 350 mg/mL.The findings of the current research showed that tiger nut has a high content of nutrients and bioactive phytochemicals.
基金Universiti Putra Malaysia for funding the present work[grant no.:IPM-9595500 and IPS-9688500]financing the first author’s postgraduate studies(Graduate Research Fellowship)MARDI Bagan Datuk Station for supplying the coconuts.
文摘Coconut water(CW)is a popular natural hydration drink in tropical countries.It is rich in essential electrolytes,vitamins,minerals,and bioactive compounds with functional properties.Therefore,this work aimed to evaluate the functional properties(i.e.,antioxidant and anti-aging activities)and to determine selected bioactive compounds in young and mature CW from the four most popular coconut varieties in Malaysia namely,Aromatic Green Dwarf(PDN),Malaysian Yellow Dwarf(MYD),MATAG(MYD×Tagnanan)and MAWA(MYD×West African Tall).Spectrophotometric and liquid chromatography methods were used to quantify the changes in the tested parameters.It is shown that the quantification of selected bioactive compounds and functional activities of CW displayed a mixed pattern at different maturation.Results from antioxidant,anti-aging activities,amino acids profile and bioactive compounds such as gallic acid,catechin,trans-zeatin,and dihydrozeatin were measured to decrease with the coconuts’maturation.Meanwhile,the amount of kinetin,kinetin riboside and trans-zeatin riboside increased with the maturation of the coconuts.Specifically,CW from young MYD showed the highest antioxidant and anti-aging activities and possessed diverse bioactive compounds that could serve as novel sources of natural functional ingredients targeted for skin anti-aging.In addition,the sensory evaluation showed that the taste of young and mature CW is acceptable for consumption with the young PDN,young MYD and mature MYD displaying the highest score.To the best of our knowledge,the present work reported the first attempt to elucidate the inhibitory activities of CW towards elastase and collagenase enzymes.
