The aim of the present work is to assess the value of Detarium Senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity o...The aim of the present work is to assess the value of Detarium Senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity of Detarium Senegalense extracts. For this purpose, sequential extraction using solvents of increasing polarity was essential. The various extracts obtained underwent phytochemical and biochemical analyses. Phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, polyphenols, anthocyanins and steroids/terpenes. Quantitative analysis of total polyphenols, total flavonoids and total anthocyanins yielded the following results: total flavonoids (0.803 ± 0029 mg EQ/100g P for acetone extract of roots and 0.871 ± 0.401 mg EQ/100g P for methanol extract of leaves);total polyphenols (23.298 ± 12.68 mg EAG/100g P for acetone extract of roots and 24.69 ± 0.49 401 mg EAG/100g P for methanol extract of leaves);total monomeric anthocyanins (44.697 ± 0.939 mg EC3G/100g P and 16.699 ± 0.193 mg EC3G/100g P respectively for acetone and methanol extracts of stem bark). DPPH free radical scavenging activity was 1.674 ± 0.023 mg/mL for the acetone extract and 0.934 ± 0.24 mg/mL for the methanol extract of roots. .展开更多
<span style="font-family:Verdana;">The use of </span><i><span style="font-family:Verdana;">Ch</span></i><i><span style="font-family:Verdana;"&...<span style="font-family:Verdana;">The use of </span><i><span style="font-family:Verdana;">Ch</span></i><i><span style="font-family:Verdana;">e</span></i><i><span style="font-family:Verdana;">nopodium</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">ambrosioides</span></i><span style="font-family:Verdana;"> in some communities in Togo reveals</span></span><span style="font-family:""> <span style="font-family:Verdana;">its importance and its medicinal properties. The plant is often infused for medicinal drinks before use. Thus, through a comparative study, this work</span><span style="font-family:Verdana;"> focused on the identification of the different phytochemical groups and then on </span><span style="font-family:Verdana;">the anti-radical capacities of ethanolic and aqueous extracts of aerial parts</span><span style="font-family:Verdana;"> (lea</span></span><span style="font-family:Verdana;">- </span><span style="font-family:""><span style="font-family:Verdana;">ves and seeds) of </span><i><span style="font-family:Verdana;">Chenopodium</span></i> <i><span style="font-family:Verdana;">ambrosiooides</span></i><span style="font-family:Verdana;"> obtained by maceration </span><span style="font-family:Verdana;">and infusion. The phytochemical tests, carried out on the various extracts, </span><span style="font-family:Verdana;">showed </span><span style="font-family:Verdana;">that they contained certain biomolecules such as flavonoids and tannins. Likewise, the presence of terpenoids and terpenes has been detected.</span><span style="font-family:Verdana;"> However, </span><span style="font-family:Verdana;">it is only in aqueous extracts that the alkaloids have been revealed. The</span><span style="font-family:Verdana;"> determination of the polyphenol content of the extracts revealed that the aqueous </span><span style="font-family:Verdana;">infusion contains 25.699 mgGAE/g DE, followed by the aqueous macera</span><span style="font-family:Verdana;">tion: 24.873 mgGAE/g DE, after the ethanolic infusion: 21.009 mg GAE/g</span><span style="font-family:Verdana;"> DE and </span><span style="font-family:Verdana;">of the ethanolic maceration: 20.625 mg GAE/g DE. The results of the</span><span style="font-family:Verdana;"> deter</span><span style="font-family:Verdana;">mination of the anti-radical capacity by the DPPH method after calculation of</span> <span><span style="font-family:Verdana;">the IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;">, revealed that the infusion gives respectively in aqueous and etha</span></span><span style="font-family:Verdana;">nolic medium: 25.541 </span></span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml and 48.269 </span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml while the maceration leads to 29.187 </span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml 50.999 </span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml respectively. This study showed that each extract reacted with DPPH and the aqueous infusion was the most reactive.</span>展开更多
Background:Among the antioxidant reactions,no correlation(R_(2)=0.5468)has been observed between the logarithm of the reciprocal of EC50 obtained by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azino-bis-3-ethylbenzothi...Background:Among the antioxidant reactions,no correlation(R_(2)=0.5468)has been observed between the logarithm of the reciprocal of EC50 obtained by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS)assays,which can measure single electron transfer reactions.Therefore,we attempted to investigate the radical scavenging reaction of each antioxidant in the DPPH and ABTS assays by using electron spin resonance(ESR),and to study an analytical method that can eliminate other than that factor.Results:Singular value decomposition(SVD)of the spectra that were obtained by ESR measurements was used to separate the components and to determine the detailed changes of each component.We calculated the EC50 from the changes in the spectra of the first and second components owing to the change in the antioxidant concentration in the DPPH and ABTS assays to identify which component contributed more to the radical scavenging reaction.The results demonstrated a positive correlation(R2=0.8806)between the EC50s of the first component of each of the DPPH and ABTS assays.In this study,common spectral changes were observed for all compounds with radical scavenging activity,so the first component was considered to be radical scavenging activity.Conclusions:Through this study,we could calculate the EC50 that significantly contributed to the radical scavenging reaction by decomposing the spectra that were obtained from ESR measurements of the reactions by the DPPH and ABTS assays into specific values.展开更多
文摘The aim of the present work is to assess the value of Detarium Senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity of Detarium Senegalense extracts. For this purpose, sequential extraction using solvents of increasing polarity was essential. The various extracts obtained underwent phytochemical and biochemical analyses. Phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, polyphenols, anthocyanins and steroids/terpenes. Quantitative analysis of total polyphenols, total flavonoids and total anthocyanins yielded the following results: total flavonoids (0.803 ± 0029 mg EQ/100g P for acetone extract of roots and 0.871 ± 0.401 mg EQ/100g P for methanol extract of leaves);total polyphenols (23.298 ± 12.68 mg EAG/100g P for acetone extract of roots and 24.69 ± 0.49 401 mg EAG/100g P for methanol extract of leaves);total monomeric anthocyanins (44.697 ± 0.939 mg EC3G/100g P and 16.699 ± 0.193 mg EC3G/100g P respectively for acetone and methanol extracts of stem bark). DPPH free radical scavenging activity was 1.674 ± 0.023 mg/mL for the acetone extract and 0.934 ± 0.24 mg/mL for the methanol extract of roots. .
文摘<span style="font-family:Verdana;">The use of </span><i><span style="font-family:Verdana;">Ch</span></i><i><span style="font-family:Verdana;">e</span></i><i><span style="font-family:Verdana;">nopodium</span></i><span style="font-family:""> <i><span style="font-family:Verdana;">ambrosioides</span></i><span style="font-family:Verdana;"> in some communities in Togo reveals</span></span><span style="font-family:""> <span style="font-family:Verdana;">its importance and its medicinal properties. The plant is often infused for medicinal drinks before use. Thus, through a comparative study, this work</span><span style="font-family:Verdana;"> focused on the identification of the different phytochemical groups and then on </span><span style="font-family:Verdana;">the anti-radical capacities of ethanolic and aqueous extracts of aerial parts</span><span style="font-family:Verdana;"> (lea</span></span><span style="font-family:Verdana;">- </span><span style="font-family:""><span style="font-family:Verdana;">ves and seeds) of </span><i><span style="font-family:Verdana;">Chenopodium</span></i> <i><span style="font-family:Verdana;">ambrosiooides</span></i><span style="font-family:Verdana;"> obtained by maceration </span><span style="font-family:Verdana;">and infusion. The phytochemical tests, carried out on the various extracts, </span><span style="font-family:Verdana;">showed </span><span style="font-family:Verdana;">that they contained certain biomolecules such as flavonoids and tannins. Likewise, the presence of terpenoids and terpenes has been detected.</span><span style="font-family:Verdana;"> However, </span><span style="font-family:Verdana;">it is only in aqueous extracts that the alkaloids have been revealed. The</span><span style="font-family:Verdana;"> determination of the polyphenol content of the extracts revealed that the aqueous </span><span style="font-family:Verdana;">infusion contains 25.699 mgGAE/g DE, followed by the aqueous macera</span><span style="font-family:Verdana;">tion: 24.873 mgGAE/g DE, after the ethanolic infusion: 21.009 mg GAE/g</span><span style="font-family:Verdana;"> DE and </span><span style="font-family:Verdana;">of the ethanolic maceration: 20.625 mg GAE/g DE. The results of the</span><span style="font-family:Verdana;"> deter</span><span style="font-family:Verdana;">mination of the anti-radical capacity by the DPPH method after calculation of</span> <span><span style="font-family:Verdana;">the IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;">, revealed that the infusion gives respectively in aqueous and etha</span></span><span style="font-family:Verdana;">nolic medium: 25.541 </span></span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml and 48.269 </span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml while the maceration leads to 29.187 </span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml 50.999 </span><span style="font-family:Verdana;">μ</span><span style="font-family:Verdana;">g/ml respectively. This study showed that each extract reacted with DPPH and the aqueous infusion was the most reactive.</span>
基金supported by JSPS KAKENHI,Japan Grant Number 17K05366.
文摘Background:Among the antioxidant reactions,no correlation(R_(2)=0.5468)has been observed between the logarithm of the reciprocal of EC50 obtained by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS)assays,which can measure single electron transfer reactions.Therefore,we attempted to investigate the radical scavenging reaction of each antioxidant in the DPPH and ABTS assays by using electron spin resonance(ESR),and to study an analytical method that can eliminate other than that factor.Results:Singular value decomposition(SVD)of the spectra that were obtained by ESR measurements was used to separate the components and to determine the detailed changes of each component.We calculated the EC50 from the changes in the spectra of the first and second components owing to the change in the antioxidant concentration in the DPPH and ABTS assays to identify which component contributed more to the radical scavenging reaction.The results demonstrated a positive correlation(R2=0.8806)between the EC50s of the first component of each of the DPPH and ABTS assays.In this study,common spectral changes were observed for all compounds with radical scavenging activity,so the first component was considered to be radical scavenging activity.Conclusions:Through this study,we could calculate the EC50 that significantly contributed to the radical scavenging reaction by decomposing the spectra that were obtained from ESR measurements of the reactions by the DPPH and ABTS assays into specific values.
