Hyperuricemia has been identified as the second most common metabolic disease,increasing the incidence of gout,hypertension,kidney disease,and cardiovascular disease.Lactic acid bacteria have been attracting considera...Hyperuricemia has been identified as the second most common metabolic disease,increasing the incidence of gout,hypertension,kidney disease,and cardiovascular disease.Lactic acid bacteria have been attracting considerable interest in anti-hyperuricemia given various probiotic functions.In this study,sixteen lactic acid bacteria were selected from one hundred ninety-two isolates through whole-cell catalysis,affordingβ-D-glucosidase activity of 0.1652-0.3346 U/mg wet cells,degradation rate of inosine-transformed rate of 1.2125-4.6555 mM/h and guanosine-transformed rate of 0.2904-1.8303 mM/h.The sixteen candidates’acid tolerance,bile resistance,hydrophobicity,and auto-aggregation were further evaluated.According to the results of the seven indexes,the five top-scored strains were viewed as the potentially functional probiotics for anti-hyperuricemia via the comprehensive evaluation of principal component analysis.Cell assay showed that the adhesion of the five strains to Caco-2 cells ranged from 2.67%to 8.67%.Moreover,the five strains can decrease the adhesion of E.coli to Caco-2 cells by 36.67-98.73%through competition inhibition,exclusion inhibition,and replacement inhibition.Finally,the five probiotics were sensitive to some of the antibiotics examined and were innocuous with nonhemolytic activity.The preliminary works indicate that the five candidates could show a good potential application against hyperuricemia.展开更多
基金The National Natural Science Foundation of China(3210189432160547)+2 种基金National Key Research and Development Program of China(2017YFD0400503-3)Vegetable Industry Technology System Post Expert Project of Jiangxi Province(JXARS-06),Major Special Science and Technology Project of Yunnan Province(202002AE320006)Research Project of State Key Laboratory of Food Science and Technology,Nanchang University(SKLF-ZZA-201910,SKLF-ZZB-202132)are gratefully acknowledged.
文摘Hyperuricemia has been identified as the second most common metabolic disease,increasing the incidence of gout,hypertension,kidney disease,and cardiovascular disease.Lactic acid bacteria have been attracting considerable interest in anti-hyperuricemia given various probiotic functions.In this study,sixteen lactic acid bacteria were selected from one hundred ninety-two isolates through whole-cell catalysis,affordingβ-D-glucosidase activity of 0.1652-0.3346 U/mg wet cells,degradation rate of inosine-transformed rate of 1.2125-4.6555 mM/h and guanosine-transformed rate of 0.2904-1.8303 mM/h.The sixteen candidates’acid tolerance,bile resistance,hydrophobicity,and auto-aggregation were further evaluated.According to the results of the seven indexes,the five top-scored strains were viewed as the potentially functional probiotics for anti-hyperuricemia via the comprehensive evaluation of principal component analysis.Cell assay showed that the adhesion of the five strains to Caco-2 cells ranged from 2.67%to 8.67%.Moreover,the five strains can decrease the adhesion of E.coli to Caco-2 cells by 36.67-98.73%through competition inhibition,exclusion inhibition,and replacement inhibition.Finally,the five probiotics were sensitive to some of the antibiotics examined and were innocuous with nonhemolytic activity.The preliminary works indicate that the five candidates could show a good potential application against hyperuricemia.
