A genetic linkage map with 89 SSR marker loci was constructed based on a maize (Zea mays L.) population consisting of 184 F-2 individuals from the cross, Huangzao 4 X Ye 107. The 184 F-3 families were evaluated in the...A genetic linkage map with 89 SSR marker loci was constructed based on a maize (Zea mays L.) population consisting of 184 F-2 individuals from the cross, Huangzao 4 X Ye 107. The 184 F-3 families were evaluated in the field under well-watered and drought-stressed regimes in Shanxi Province of China. The objectives of the study were to identify genetic segments responsible for the expression of anthesis-silking interval (ASI), ear setting and grain yield, and to examine if the quantitative trait loci (QTLs) for ASI or yield components can be used in marker-assisted selection (MAS) to improve grain yield under drought conditions. Results showed that under well-watered and drought-stressed regimes, three and two QTLs involved in the expression of ASI were detected on chromosomes 1, 2 and 3, and 2 and 5, respectively. Under well-watered regime, two QTLs for ear setting were detected on chromosomes 3 and 6, explaining about 19.9% of the phenotypic variance, and displayed additive and partial dominant effects, respectively. Under drought-stressed condition, four QTLs for ear setting were detected on chromosomes 3, 7 and 10, which were responsible for interpreting 60.4% of the phenotypic variance, and showed dominant or partial dominant effects. Under well-watered condition, four QTLs controlling grain yield were identified on chromosomes 3, 6 and 7, while five QTLs were identified under drought stress on chromosomes 1, 2, 4 and 8. The gene action was of additive or partial dominant effects, and each QTL could explain 7.3% to 22.0% of the phenotypic variance, respectively. Under drought conditions, ASI and ear setting percentage were highly correlated with grain yield, which can be used as secondary traits for grain yield selection. Based on linked markers detected and gene action analyzed, an MAS strategy for yield improvement under drought condition could be established, which consists of QTLs contributing to decreased ASI and to increased ear setting and grain yield, respectively.展开更多
A two-year field experiment was conducted to evaluate the effects of plant density on tassel and ear differentiation, anthesissilking interval(ASI), and grain yield formation of two types of modern maize hybrids(Zhong...A two-year field experiment was conducted to evaluate the effects of plant density on tassel and ear differentiation, anthesissilking interval(ASI), and grain yield formation of two types of modern maize hybrids(Zhongdan 909(ZD909) as tolerant hybrid to crowding stress, Jidan 209(JD209) and Neidan 4(ND4) as intolerant hybrids to crowding stress) in Northeast China. Plant densities of 4.50×104(D1), 6.75×104(D2), 9.00×104(D3), 11.25×104(D4), and 13.50×104(D5) plants ha-1had no significant effects on initial time of tassel and ear differentiation of maize. Instead, higher plant density delayed the tassel and ear development during floret differentiation and sexual organ formation stage, subsequently resulting in ASI increments at the rate of 1.2–2.9 days on average for ZD909 in 2013–2014, 0.7–4.2 days for JD209 in 2013, and 0.5–3.7 days for ND4 in 2014, respectively, under the treatments of D2, D3, D4, and D5 compared to that under the D1 treatment. Total florets, silking florets, and silking rates of ear showed slightly decrease trends with the plant density increasing, whereas the normal kernels seriously decreased at the rate of 11.0–44.9% on average for ZD909 in 2013–2014, 2.0–32.6% for JD209 in 2013, and 9.7–28.3% for ND4 in 2014 with the plant density increased compared to that under the D1 treatment due to increased florets abortive rates. It was also observed that 100-kernel weight of ZD909 showed less decrease trend compared that of JD209 and ND4 along with the plant densities increase. As a consequence, ZD909 gained its highest grain yield by 13.7 t ha-1on average at the plant density of 9.00×104 plants ha-1, whereas JD209 and ND4 reached their highest grain yields by 11.7 and 10.2 t ha-1at the plant density of 6.75×104 plants ha-1, respectively. Our experiment demonstrated that hybrids with lower ASI, higher kernel number potential per ear, and relative constant 100-kernel weight(e.g., ZD909) could achieve higher yield under dense planting in high latitude area(e.g., Northeast China).展开更多
Drought, like many other environmental stresses, has adverse effects on crop yield including maize (Zea mays L.). Low water availability is one of the major causes for maize yield reductions affecting the majority of ...Drought, like many other environmental stresses, has adverse effects on crop yield including maize (Zea mays L.). Low water availability is one of the major causes for maize yield reductions affecting the majority of the farmed regions around the world. Therefore, the development of drought-tolerant lines becomes increasingly more important. In maize, a major effect of water stress is a delay in silking, resulting in an increase in the anthesis-silking interval, which is an important cause of yield failures. Diverse strategies are used by breeding programs to improve drought tolerance. Conventional breeding has improved the drought tolerance of temperate maize hybrids and the use of managed drought environments, accurate phenotyping, and the identification and deployment of secondary traits has been effective in improving the drought tolerance of tropical maize populations and hybrids as well. The contribution of molecular biology will be potential to identify key genes involved in metabolic pathways related to the stress response. Functional genomics, reverse and forward genetics, and comparative genomics are all being deployed with a view to achieving these goals. However, a multidisciplinary approach, which ties together breeding, physiology and molecular genetics, can bring a synergistic understanding to the response of maize to water deficit and improve the breeding efficiency.展开更多
文摘A genetic linkage map with 89 SSR marker loci was constructed based on a maize (Zea mays L.) population consisting of 184 F-2 individuals from the cross, Huangzao 4 X Ye 107. The 184 F-3 families were evaluated in the field under well-watered and drought-stressed regimes in Shanxi Province of China. The objectives of the study were to identify genetic segments responsible for the expression of anthesis-silking interval (ASI), ear setting and grain yield, and to examine if the quantitative trait loci (QTLs) for ASI or yield components can be used in marker-assisted selection (MAS) to improve grain yield under drought conditions. Results showed that under well-watered and drought-stressed regimes, three and two QTLs involved in the expression of ASI were detected on chromosomes 1, 2 and 3, and 2 and 5, respectively. Under well-watered regime, two QTLs for ear setting were detected on chromosomes 3 and 6, explaining about 19.9% of the phenotypic variance, and displayed additive and partial dominant effects, respectively. Under drought-stressed condition, four QTLs for ear setting were detected on chromosomes 3, 7 and 10, which were responsible for interpreting 60.4% of the phenotypic variance, and showed dominant or partial dominant effects. Under well-watered condition, four QTLs controlling grain yield were identified on chromosomes 3, 6 and 7, while five QTLs were identified under drought stress on chromosomes 1, 2, 4 and 8. The gene action was of additive or partial dominant effects, and each QTL could explain 7.3% to 22.0% of the phenotypic variance, respectively. Under drought conditions, ASI and ear setting percentage were highly correlated with grain yield, which can be used as secondary traits for grain yield selection. Based on linked markers detected and gene action analyzed, an MAS strategy for yield improvement under drought condition could be established, which consists of QTLs contributing to decreased ASI and to increased ear setting and grain yield, respectively.
基金supported by the National Basic Research Program of China (2015CB150404)the National Natural Science Foundation of China (31671642)+1 种基金the Key Program of Science and Technology Department of Jilin Province, China (LFGC14205)the Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-XTCX2016008)
文摘A two-year field experiment was conducted to evaluate the effects of plant density on tassel and ear differentiation, anthesissilking interval(ASI), and grain yield formation of two types of modern maize hybrids(Zhongdan 909(ZD909) as tolerant hybrid to crowding stress, Jidan 209(JD209) and Neidan 4(ND4) as intolerant hybrids to crowding stress) in Northeast China. Plant densities of 4.50×104(D1), 6.75×104(D2), 9.00×104(D3), 11.25×104(D4), and 13.50×104(D5) plants ha-1had no significant effects on initial time of tassel and ear differentiation of maize. Instead, higher plant density delayed the tassel and ear development during floret differentiation and sexual organ formation stage, subsequently resulting in ASI increments at the rate of 1.2–2.9 days on average for ZD909 in 2013–2014, 0.7–4.2 days for JD209 in 2013, and 0.5–3.7 days for ND4 in 2014, respectively, under the treatments of D2, D3, D4, and D5 compared to that under the D1 treatment. Total florets, silking florets, and silking rates of ear showed slightly decrease trends with the plant density increasing, whereas the normal kernels seriously decreased at the rate of 11.0–44.9% on average for ZD909 in 2013–2014, 2.0–32.6% for JD209 in 2013, and 9.7–28.3% for ND4 in 2014 with the plant density increased compared to that under the D1 treatment due to increased florets abortive rates. It was also observed that 100-kernel weight of ZD909 showed less decrease trend compared that of JD209 and ND4 along with the plant densities increase. As a consequence, ZD909 gained its highest grain yield by 13.7 t ha-1on average at the plant density of 9.00×104 plants ha-1, whereas JD209 and ND4 reached their highest grain yields by 11.7 and 10.2 t ha-1at the plant density of 6.75×104 plants ha-1, respectively. Our experiment demonstrated that hybrids with lower ASI, higher kernel number potential per ear, and relative constant 100-kernel weight(e.g., ZD909) could achieve higher yield under dense planting in high latitude area(e.g., Northeast China).
文摘Drought, like many other environmental stresses, has adverse effects on crop yield including maize (Zea mays L.). Low water availability is one of the major causes for maize yield reductions affecting the majority of the farmed regions around the world. Therefore, the development of drought-tolerant lines becomes increasingly more important. In maize, a major effect of water stress is a delay in silking, resulting in an increase in the anthesis-silking interval, which is an important cause of yield failures. Diverse strategies are used by breeding programs to improve drought tolerance. Conventional breeding has improved the drought tolerance of temperate maize hybrids and the use of managed drought environments, accurate phenotyping, and the identification and deployment of secondary traits has been effective in improving the drought tolerance of tropical maize populations and hybrids as well. The contribution of molecular biology will be potential to identify key genes involved in metabolic pathways related to the stress response. Functional genomics, reverse and forward genetics, and comparative genomics are all being deployed with a view to achieving these goals. However, a multidisciplinary approach, which ties together breeding, physiology and molecular genetics, can bring a synergistic understanding to the response of maize to water deficit and improve the breeding efficiency.
