Objective： Antrodia camphorata （AC）, a precious medicinal mushroom in Taiwan, is popularly used for adjuvant cancer therapy. This paper aims to clarify the metabolites which are present in tumor tissues after oral ...Objective： Antrodia camphorata （AC）, a precious medicinal mushroom in Taiwan, is popularly used for adjuvant cancer therapy. This paper aims to clarify the metabolites which are present in tumor tissues after oral administration of AC in Sarcoma-180 tumor-bearing mice, as well as their contents in tumors. Methods： Tumors of Sarcoma-180 tumor-bearing mice were obtained at 1 h and 4 h after oral administration of AC extract, and the metabolites in the tumor homogenate samples were characterized using UHPLC-orbitrap/MS analysis. Then, a fully validated LC-MS/MS method was developed for quantitative analysis of the most abundant compounds in tumor tissues, namely （25R/S）-antcin H. Results： A total of 33 compounds were characterized in tumor homogenate samples including 28 prototypes of triterpenoids and 5 metabolites. Among them, （25R）-antcin H and （25S）-antcin H had the highest contents of 2.03 and 0.66 μg/g tumor tissues for the 1 h group, and 2.04 and 0.59 μg/g tumor tissues for the 4 h group, respectively. It was obvious that （25R）-antcin H had higher tumor affinity than （25S）-antcin H, since the content of （25R）-antcin H was lower than that of （25S）-antcin H in AC extract （P 〈 0.01）. Conclusion： Triterpenoids can enter tumor tissues after oral administration of AC. Particularly, （25R）-antcin H showed higher exposure to tumor than （25S）-antcin H. These compounds could contribute to the anticancer activities of AC.展开更多
基金This work was supported by National Natural Science Foundation of China （No. 81303294）, the National Key Research and Development Program of China （No. 2017YFC1700405）, and Young Elite Scientists Sponsorship Program by CAST （2016QNRC001）.
文摘Objective： Antrodia camphorata （AC）, a precious medicinal mushroom in Taiwan, is popularly used for adjuvant cancer therapy. This paper aims to clarify the metabolites which are present in tumor tissues after oral administration of AC in Sarcoma-180 tumor-bearing mice, as well as their contents in tumors. Methods： Tumors of Sarcoma-180 tumor-bearing mice were obtained at 1 h and 4 h after oral administration of AC extract, and the metabolites in the tumor homogenate samples were characterized using UHPLC-orbitrap/MS analysis. Then, a fully validated LC-MS/MS method was developed for quantitative analysis of the most abundant compounds in tumor tissues, namely （25R/S）-antcin H. Results： A total of 33 compounds were characterized in tumor homogenate samples including 28 prototypes of triterpenoids and 5 metabolites. Among them, （25R）-antcin H and （25S）-antcin H had the highest contents of 2.03 and 0.66 μg/g tumor tissues for the 1 h group, and 2.04 and 0.59 μg/g tumor tissues for the 4 h group, respectively. It was obvious that （25R）-antcin H had higher tumor affinity than （25S）-antcin H, since the content of （25R）-antcin H was lower than that of （25S）-antcin H in AC extract （P 〈 0.01）. Conclusion： Triterpenoids can enter tumor tissues after oral administration of AC. Particularly, （25R）-antcin H showed higher exposure to tumor than （25S）-antcin H. These compounds could contribute to the anticancer activities of AC.
