This study investigates the compatibility and efficacy of combining ammonium molybdate(AM)with antagonistic bacteria Bacillus amyloliquefaciens B10W10 and Pseudomonas sp.B11W11 for brown rot control(Monilinia laxa).In...This study investigates the compatibility and efficacy of combining ammonium molybdate(AM)with antagonistic bacteria Bacillus amyloliquefaciens B10W10 and Pseudomonas sp.B11W11 for brown rot control(Monilinia laxa).In vitro experiments reveal variable mycelial growth inhibition rates compared to untreated controls,with B11W11+0.5%AM and B10W10+2%AM displaying the highest inhibition rates after 5 days.After 10 days,the 2%AM+B10W10 combination exhibits the highest inhibition rate.Microscopic observations show structural alterations in mycelium within inhibition zones,marked by vacuolization.The antagonistic bacteria,alone or with different ammonium molybdate concentrations,significantly impact M.laxa spore germination,with the B10W10 cell filtrate+2%ammonium molybdate combination achieving the most substantial inhibition.Conversely,the 0.5%ammonium molybdate treatment has the lowest inhibition rate while the combination of AM and bacteria is giving better results compared to the use of bacteria alone.Fruits treated with various antagonistic bacteria and ammonium molybdate combinations demonstrate a significant reduction in disease severity.The 0.5%AM+B10W10 combination exhibits the lowest severity.FT-IR spectra analysis identifies shifts in fungal biomass functional groups,with reduced lignin-related bands and increased phenols,lipids,polysaccharides,and carbohydrates.This highlights the structural modifications caused by the biological treatments.The study also evaluates the effects on fruit quality parameters.The 2%ammonium molybdate treatment yields the lowest weight loss.TSS levels are affected by salt concentration,while acid content remains consistent across treatments.All treatments influence fruit firmness compared to controls.These findings emphasize the potential of combining ammonium molybdate and antagonistic bacteria for effective brown rot control,highlighting their compatibility and effects on disease severity,fungal biomass,spore germination,and fruit quality.展开更多
Oral ulcers(OUs)are among the most common lesions of the oral mucosa,typically associated with pain and burning sensations,and remain clinically challenging due to the scarcity of effective treatment options.Cy_(RL-QN...Oral ulcers(OUs)are among the most common lesions of the oral mucosa,typically associated with pain and burning sensations,and remain clinically challenging due to the scarcity of effective treatment options.Cy_(RL-QN15),a novel ultra-short cyclic heptapeptide recently shown to promote skin repair,diabetic wound healing,and follicle neogenesis,was evaluated for its therapeutic potential in mucosal repair.Using a rat OU model and a primary oral epithelial cell inflammation model,Cy_(RL-QN15)significantly accelerated wound closure through coordinated modulation of immune-epithelial crosstalk,including suppression of inflammatory cytokine release from macrophages and neutrophils,reduction of pro-inflammatory factor secretion by oral epithelial cells,and enhancement of their proliferation and migration.Mechanistic studies employing alanine scanning mutagenesis and microscale thermophoresis revealed that Cy_(RL-QN15)directly interacted with Toll-like receptor 4(TLR4)via a methionine-dependent binding interface(K_(d)=2.64μmol/L),thereby inhibiting downstream MyD88/NF-κB signaling.As the first ultra-short cyclic heptapeptide identified to antagonize TLR4,Cy_(RL-QN15)represents a mechanistically distinct immunomodulatory scaffold that restores mucosal homeostasis and offers a promising therapeutic candidate for TLR4-based OU intervention.展开更多
[Objective]The aim of this study was to screen the antagonistic spore-forming bacteria of Verticillium dahliae and identify its physiological and biochemical characteristics.[Method]Taking the cotton verticillium wilt...[Objective]The aim of this study was to screen the antagonistic spore-forming bacteria of Verticillium dahliae and identify its physiological and biochemical characteristics.[Method]Taking the cotton verticillium wilt pathogen Verticillium dahliae V-190 as the test microorganism,the antagonistic spore-forming bacteria were screened.Through the preliminary screening and secondary screening,an antagonistic bacteria strain 7-30 with stronger antibacterial activity was obtained,and its morphological characteristics,physiological and biochemical characteristics were also identified.[Result]84 antagonistic bacteria strains were isolated from soil in various places by the preliminary screening.Especially,18 strains with better antagonistic ability were screened again,so an antagonistic bacteria strain 7-30 with the diameter of inhibition zone 18.9 mm and stronger antibacterial activity was obtained.According to its morphological characteristics,physiological and biochemical characteristics,the antagonistic bacteria strain 7-30 was identified as Bacillus subtilis primarily.[Conclusion]The strain 7-30 was obtained as the antagonistic spore-forming bacteria of Verticillium dahliae.展开更多
[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applyin...[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil.[Method] Actinomycete strains,fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method,then its resistance was screened respectively by the improved confront culture method after colonies were purified.Finally,bacteria strains with better antagonistic effect were identified.[Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples,which contained 15 fluorescent bacteria strains,5 bacillus strains and 9 actinomycete strains.Furthermore,three antagonistic bacteria strains of FM2,FM3 and FM4 with the strongest antagonism were identified primarily.[Conclusion] According to cultural characteristics,morphological observation,biochemical and physiological tests,FM2 belongs to bacillus subtilis,while FM3 and FM4 belong to micrococcus.展开更多
[ Objective ] The paper was to screen bacterial strain with significant antagonistic effect against Phytophthora infestans, so as to provide basis for further development and utilization of antagonistic bacteria to in...[ Objective ] The paper was to screen bacterial strain with significant antagonistic effect against Phytophthora infestans, so as to provide basis for further development and utilization of antagonistic bacteria to inhibit P. infestans and control potato late bright. [ Method] Plate dual culture and filter paper method were used to determine the inhibition effect of strains in vivo, fermentation broth and bacterial liquid of 61 strains against P. infestans and the resistance-induction effect of SR13-2 strain. [ Result] The inhibition rate of 24 strains among 61 tested strains against mycelial growth of P. infestans was greater than 60%, and the inhibi- tion effect of HT-6 strain was the strongest with the inhibition rate of 89.92%. However, fermentation broth of all tested strains had no significant inhibition effect against P. infestans, while the inhibition effect of bacterial liquid of most strains was significantly higher than strain in vivo; the inhibition effect of $34-1 strain was the strongest with inhibition rate of 91.50%. The bacterial liquid of SR13-2 strain was found to have significant resistance-induction effect with protective rate of 60%. [ Conclusion] The inhibition effect of strains in vivo and fermentation broth of antagonistic strains S34-1 and SR13-2 had no relationship with each other, while bacterial liquid had great application potential in controlling potato late bright.展开更多
[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The anta...[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture method.The SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,respectively.The fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial activity.The antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was 5.5-10.0.The diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of A.konjac was obtained.展开更多
[Objective] This study was conducted to screen resources for biological control of soil-borne diseases of tomato. [Method] Antagonistic bacteria against Ral- stonia solanacearum and Fusarium oxysporum were isolated an...[Objective] This study was conducted to screen resources for biological control of soil-borne diseases of tomato. [Method] Antagonistic bacteria against Ral- stonia solanacearum and Fusarium oxysporum were isolated and purified from soil samples by dilution plating and confronting incubation on PDA plates, then the antibacterial spectrum and metabolic secretions of the bacterial isolates were measured, and their species were identified by establishing phylogenetic trees with Neighbor-Joining method. [Result] From the 60 soil samples, 10 of 59 antagonistic bacteria isolates against R. solanacearum showed inhibition zone diameter 〉 25 mm, and 4 of 30 strains against F. oxysporum exhibited inhibition rate 〉 30%. The bacteriostatic substances and antibacterial spectrum analysis on above 14 isolates revealed that four strains N23-2, N58-2, NF59-3 and NF61-1 had good antago- nism against pathogenic bacteria and fungi; 12 strains produced cellulose, 11 strains produced proteases, and 6 strains produced siderophores. The molecular identification experiments indicated that four strains were members of Paenibacillus, three strains of Streptomyces, three strains of Pseudomonas strains, and four strains of Bacillus strains.展开更多
A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRN...A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios： Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows： KB culture medium （peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL）; temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% （v/v）.展开更多
[ Objective] The paper was to screen the antagonistic strain against Phytophthora sojae with biocontrol potential, and provide basis for searching control measures and designing new control strategies against P. sojae...[ Objective] The paper was to screen the antagonistic strain against Phytophthora sojae with biocontrol potential, and provide basis for searching control measures and designing new control strategies against P. sojae. [ Method] The rhizosphere soil of soybean was collected from three different places in Heilongjiang Province, and various soil microorganisms were isolated. Dual culture method was used to screen the microorganism with antagonistic effect against P. sojae. On this basis, the growth inhibition rate of the microorganism with stronger antagonistic effect against P. sojae was determined, and its control effect against P. sojae was also measured. [ Result] A strain of bacterium with relatively good antagonistic effect was isolated from soil, and named as strain B048. Dual test showed that the growth inhibition rate of antagonistic bacterium 11048 against P. sojac reached 97.5%. Antagonistic endurance tests showed that the width of inhibition zone was still 20.0 mm after dual culture with P. sojac for21 d. In potting experiment, the control effect of B048 against P. sojae was 100%. The antagonistic bacterium was primarily identified to be Bacillus pumilus through morphology and 16S rDNA sequence analysis. [Condusion] The antagonistic bacterium B048 had good prospect to be developed as the biocontrol bacterium against P. sojae.展开更多
[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promo...[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.展开更多
[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, ferment...[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.展开更多
Seven known dibenzocyclooctadiene lignans,tigloylgomisin P(1), angeloylgomisin P(2),gomisin A(3),Schizandrin(4),angeloylgomisin H(5),gomisin B(6) and R(+)-gomisin M_1(7)were isolated from Kadsura heteroclita.The confo...Seven known dibenzocyclooctadiene lignans,tigloylgomisin P(1), angeloylgomisin P(2),gomisin A(3),Schizandrin(4),angeloylgomisin H(5),gomisin B(6) and R(+)-gomisin M_1(7)were isolated from Kadsura heteroclita.The conformation of tiglolylgomisin P(1)was established by 2D NMR techniques.Using[~3H]platelet activating factor(PAF)binding to human platelet membrane assay,(1),(2)and(7)showed PAF receptor antagonistic activities.展开更多
Aflatoxin is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species,and can seriously threaten the health of consumers,thus becoming a global concern.Corn,as an impor...Aflatoxin is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species,and can seriously threaten the health of consumers,thus becoming a global concern.Corn,as an important oil and economic crop,is highly susceptible contaminated by aflatoxin.In this study,antagonistic bacteria with strong inhibitory effect on aflatoxin were screened to provide support for the treatment aflatoxin contamination control in corn.Ten strains which have strong antagonistic effects against A.flavus were isolated from healthy corn from different corn producing areas in China.Among them,the antagonistic bacteria JTFM1001 through corn kernels in vivo and field experiment,the inhibition effect of aflatoxin contamination reached above 70%and 55%,respectively.And the strain was identified as Bacillus subtilis based on its morphological,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA.In addition,our data showed that it can colonize in the rhizosphere and survive for a long time,forming the dominant flora,with broad application prospect.Finally,we were surprised to find that the antibacterial metabolites secreted by the antagonistic bacteria was one of the mechanisms of its inhibition of A.flavus and aflatoxin.This will provide us with new ideas and perspectives on the effective prevention and control of aflatoxin contamination in corn and corn oil.展开更多
It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%, and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%, lower than that of ...It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%, and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%, lower than that of DZW-47. The inhibitory mycelia growth mechanism of different strains on R.solani was quite different, with DZW-3 mainly on the aspect of hyperparasitism, DZW-21 on the synergism of hyperparasitism and metabolite, DZW-47 on the synergism of nutrient competition and secondary metabolite, ZLR-2 and ZLR-11 on producing secondary metabolite. Controlling efficiency of seedling bed accorded basically with that of the broth. The controlling efficiency of DZW-47, ZLR-2, ZLR-11, DZW-21 and DZW-3 were 97.20%, 95.7%, 94.6%, 93.6% and 89.20%, respectively.展开更多
[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae...[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae, and carry out sequence measurement and phylogenetic analysis on 10 representative strains. [ Meth- od] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried out genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and 2 strains with independent groups at Wat- son distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively. [ Conclu- sion ] The genetic diversity of 48 strains of bacteria was obvious. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum and V. dahliae, which could also provide new strain sources for biological control of diseases in cotton production.展开更多
Feng Fengyi , Zhang Pin , He Youjian 1, Li Yuhong1 , Zhou Meizhen2 , Chen Gang2 and Li Lin2 The Cancer Hospital of the CAMS & PUMC, Beijing 1000211 The Cancer Hospital of Sun Yat Sen University of Medical Science...Feng Fengyi , Zhang Pin , He Youjian 1, Li Yuhong1 , Zhou Meizhen2 , Chen Gang2 and Li Lin2 The Cancer Hospital of the CAMS & PUMC, Beijing 1000211 The Cancer Hospital of Sun Yat Sen University of Medical Sciences, Guangzhou 5100602Beijing Hospital of the Ministry of Health, Beijing 100730展开更多
Aim and methods The study of three-dimensional quantitative structure-activity relationship （3D-QSAR） of DDPH and its derivatives has been performed using Apex-3D programme. Results The result indicates that substit...Aim and methods The study of three-dimensional quantitative structure-activity relationship （3D-QSAR） of DDPH and its derivatives has been performed using Apex-3D programme. Results The result indicates that substituents of para- and ortho-positions in phenyl ring of aryloxyalkylamine greatly influence the bioactivity. Conclusion The biophore model and 3D-QSAR equation help us not only further understand receptor-ligand interactions, but also design new compounds with better bioactivity.展开更多
[ Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field. [Method] More than 200 strains of bacteria which could produce spore wer...[ Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field. [Method] More than 200 strains of bacteria which could produce spore were isolated from soil in different places. Through screening and rescreening, the bacteria with higher antibacterial activity were conducted observation about thallus shapes and colony characters, a series of physiological and biochemical tests were performed. [Result] Rescreening results indicated that the strains including ZG-10, ZG-19, ZG-59, ZG-72 and ZG-31 had significant antibacterial activity, which had very high research value and good application prospect for biocontrol on spot blotch disease in pakchoi; the strain ZG-10 was identified to be Bacillus subtilis. [ Conclusion] The strain ZG-10 had biocontrol potential and good development prospect. This research laid certain basis for subsequent research and strated a new way for the application of antagonistic strain and proteinum polypeptide in agdculture.展开更多
基金supported by the Phytopathology Unit of the Department of Plant Protection(ENA-Meknes,Morocco).
文摘This study investigates the compatibility and efficacy of combining ammonium molybdate(AM)with antagonistic bacteria Bacillus amyloliquefaciens B10W10 and Pseudomonas sp.B11W11 for brown rot control(Monilinia laxa).In vitro experiments reveal variable mycelial growth inhibition rates compared to untreated controls,with B11W11+0.5%AM and B10W10+2%AM displaying the highest inhibition rates after 5 days.After 10 days,the 2%AM+B10W10 combination exhibits the highest inhibition rate.Microscopic observations show structural alterations in mycelium within inhibition zones,marked by vacuolization.The antagonistic bacteria,alone or with different ammonium molybdate concentrations,significantly impact M.laxa spore germination,with the B10W10 cell filtrate+2%ammonium molybdate combination achieving the most substantial inhibition.Conversely,the 0.5%ammonium molybdate treatment has the lowest inhibition rate while the combination of AM and bacteria is giving better results compared to the use of bacteria alone.Fruits treated with various antagonistic bacteria and ammonium molybdate combinations demonstrate a significant reduction in disease severity.The 0.5%AM+B10W10 combination exhibits the lowest severity.FT-IR spectra analysis identifies shifts in fungal biomass functional groups,with reduced lignin-related bands and increased phenols,lipids,polysaccharides,and carbohydrates.This highlights the structural modifications caused by the biological treatments.The study also evaluates the effects on fruit quality parameters.The 2%ammonium molybdate treatment yields the lowest weight loss.TSS levels are affected by salt concentration,while acid content remains consistent across treatments.All treatments influence fruit firmness compared to controls.These findings emphasize the potential of combining ammonium molybdate and antagonistic bacteria for effective brown rot control,highlighting their compatibility and effects on disease severity,fungal biomass,spore germination,and fruit quality.
基金supported by the National Natural Science Foundation of China(32301054,82404582,32360138,32060212)First-Class Discipline Team of Skin&Mucosal Regenerative Medicine of Kunming Medical University(2024XKTDTS10)+2 种基金Key Program of Yunnan Fundamental Research Project(202301AS070036)Open Research Fund of Yunnan Characteristic Plant Extraction Laboratory(YKKF2024003)Project of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(202301AY070001-301,202401AY070001-028,202301AY070001-012,202401AY070001-068,202301AY070001-169)。
文摘Oral ulcers(OUs)are among the most common lesions of the oral mucosa,typically associated with pain and burning sensations,and remain clinically challenging due to the scarcity of effective treatment options.Cy_(RL-QN15),a novel ultra-short cyclic heptapeptide recently shown to promote skin repair,diabetic wound healing,and follicle neogenesis,was evaluated for its therapeutic potential in mucosal repair.Using a rat OU model and a primary oral epithelial cell inflammation model,Cy_(RL-QN15)significantly accelerated wound closure through coordinated modulation of immune-epithelial crosstalk,including suppression of inflammatory cytokine release from macrophages and neutrophils,reduction of pro-inflammatory factor secretion by oral epithelial cells,and enhancement of their proliferation and migration.Mechanistic studies employing alanine scanning mutagenesis and microscale thermophoresis revealed that Cy_(RL-QN15)directly interacted with Toll-like receptor 4(TLR4)via a methionine-dependent binding interface(K_(d)=2.64μmol/L),thereby inhibiting downstream MyD88/NF-κB signaling.As the first ultra-short cyclic heptapeptide identified to antagonize TLR4,Cy_(RL-QN15)represents a mechanistically distinct immunomodulatory scaffold that restores mucosal homeostasis and offers a promising therapeutic candidate for TLR4-based OU intervention.
基金Supported by National Science Foundation of Hebei Province(398152)~~
文摘[Objective]The aim of this study was to screen the antagonistic spore-forming bacteria of Verticillium dahliae and identify its physiological and biochemical characteristics.[Method]Taking the cotton verticillium wilt pathogen Verticillium dahliae V-190 as the test microorganism,the antagonistic spore-forming bacteria were screened.Through the preliminary screening and secondary screening,an antagonistic bacteria strain 7-30 with stronger antibacterial activity was obtained,and its morphological characteristics,physiological and biochemical characteristics were also identified.[Result]84 antagonistic bacteria strains were isolated from soil in various places by the preliminary screening.Especially,18 strains with better antagonistic ability were screened again,so an antagonistic bacteria strain 7-30 with the diameter of inhibition zone 18.9 mm and stronger antibacterial activity was obtained.According to its morphological characteristics,physiological and biochemical characteristics,the antagonistic bacteria strain 7-30 was identified as Bacillus subtilis primarily.[Conclusion]The strain 7-30 was obtained as the antagonistic spore-forming bacteria of Verticillium dahliae.
基金Supported by Scientific Research and Development Fund of Hefei University of Technology(070602F)Research Funding Project for Young Teachers in Colleges of Anhui Province(2008JQ1009)~~
文摘[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil.[Method] Actinomycete strains,fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method,then its resistance was screened respectively by the improved confront culture method after colonies were purified.Finally,bacteria strains with better antagonistic effect were identified.[Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples,which contained 15 fluorescent bacteria strains,5 bacillus strains and 9 actinomycete strains.Furthermore,three antagonistic bacteria strains of FM2,FM3 and FM4 with the strongest antagonism were identified primarily.[Conclusion] According to cultural characteristics,morphological observation,biochemical and physiological tests,FM2 belongs to bacillus subtilis,while FM3 and FM4 belong to micrococcus.
基金Supported by Natural Science Foundation of Heibei Province(C2011201003)~~
文摘[ Objective ] The paper was to screen bacterial strain with significant antagonistic effect against Phytophthora infestans, so as to provide basis for further development and utilization of antagonistic bacteria to inhibit P. infestans and control potato late bright. [ Method] Plate dual culture and filter paper method were used to determine the inhibition effect of strains in vivo, fermentation broth and bacterial liquid of 61 strains against P. infestans and the resistance-induction effect of SR13-2 strain. [ Result] The inhibition rate of 24 strains among 61 tested strains against mycelial growth of P. infestans was greater than 60%, and the inhibi- tion effect of HT-6 strain was the strongest with the inhibition rate of 89.92%. However, fermentation broth of all tested strains had no significant inhibition effect against P. infestans, while the inhibition effect of bacterial liquid of most strains was significantly higher than strain in vivo; the inhibition effect of $34-1 strain was the strongest with inhibition rate of 91.50%. The bacterial liquid of SR13-2 strain was found to have significant resistance-induction effect with protective rate of 60%. [ Conclusion] The inhibition effect of strains in vivo and fermentation broth of antagonistic strains S34-1 and SR13-2 had no relationship with each other, while bacterial liquid had great application potential in controlling potato late bright.
基金Supported by Key Laboratory Fund in Hubei Province(2008NP11)Science and Technology Research and Development Project in Yichang City(A09302-17)~~
文摘[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture method.The SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,respectively.The fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial activity.The antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was 5.5-10.0.The diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of A.konjac was obtained.
文摘[Objective] This study was conducted to screen resources for biological control of soil-borne diseases of tomato. [Method] Antagonistic bacteria against Ral- stonia solanacearum and Fusarium oxysporum were isolated and purified from soil samples by dilution plating and confronting incubation on PDA plates, then the antibacterial spectrum and metabolic secretions of the bacterial isolates were measured, and their species were identified by establishing phylogenetic trees with Neighbor-Joining method. [Result] From the 60 soil samples, 10 of 59 antagonistic bacteria isolates against R. solanacearum showed inhibition zone diameter 〉 25 mm, and 4 of 30 strains against F. oxysporum exhibited inhibition rate 〉 30%. The bacteriostatic substances and antibacterial spectrum analysis on above 14 isolates revealed that four strains N23-2, N58-2, NF59-3 and NF61-1 had good antago- nism against pathogenic bacteria and fungi; 12 strains produced cellulose, 11 strains produced proteases, and 6 strains produced siderophores. The molecular identification experiments indicated that four strains were members of Paenibacillus, three strains of Streptomyces, three strains of Pseudomonas strains, and four strains of Bacillus strains.
基金supported by the Scientific Research Foundation of Xiamen Marine Research and Development Institute (No. K10102 (1))
文摘A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios： Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows： KB culture medium （peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL）; temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% （v/v）.
基金Supported by National Natural Science Foundation of China(30800040)Excellent Youth Science and Technology Fund of Anhui Province(10040606Y04)教育部留学回国人员科研启动基金资助项目~~
文摘[ Objective] The paper was to screen the antagonistic strain against Phytophthora sojae with biocontrol potential, and provide basis for searching control measures and designing new control strategies against P. sojae. [ Method] The rhizosphere soil of soybean was collected from three different places in Heilongjiang Province, and various soil microorganisms were isolated. Dual culture method was used to screen the microorganism with antagonistic effect against P. sojae. On this basis, the growth inhibition rate of the microorganism with stronger antagonistic effect against P. sojae was determined, and its control effect against P. sojae was also measured. [ Result] A strain of bacterium with relatively good antagonistic effect was isolated from soil, and named as strain B048. Dual test showed that the growth inhibition rate of antagonistic bacterium 11048 against P. sojac reached 97.5%. Antagonistic endurance tests showed that the width of inhibition zone was still 20.0 mm after dual culture with P. sojac for21 d. In potting experiment, the control effect of B048 against P. sojae was 100%. The antagonistic bacterium was primarily identified to be Bacillus pumilus through morphology and 16S rDNA sequence analysis. [Condusion] The antagonistic bacterium B048 had good prospect to be developed as the biocontrol bacterium against P. sojae.
基金Supported by Natural Science Foundation of Chongqing City(CSTC,2009BB1294)~~
文摘[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.
基金Supported by Applied Foundation Project of Science and Technology Department of Sichuan Province (2008JY0021-2)~~
文摘[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.
基金This project was supported by National Natural Science Foundation of China.
文摘Seven known dibenzocyclooctadiene lignans,tigloylgomisin P(1), angeloylgomisin P(2),gomisin A(3),Schizandrin(4),angeloylgomisin H(5),gomisin B(6) and R(+)-gomisin M_1(7)were isolated from Kadsura heteroclita.The conformation of tiglolylgomisin P(1)was established by 2D NMR techniques.Using[~3H]platelet activating factor(PAF)binding to human platelet membrane assay,(1),(2)and(7)showed PAF receptor antagonistic activities.
基金supported by the 2020-Innovation Project-Yi An,and Applied basic research plan of Hebei province-key basic research projects(17967502D).
文摘Aflatoxin is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species,and can seriously threaten the health of consumers,thus becoming a global concern.Corn,as an important oil and economic crop,is highly susceptible contaminated by aflatoxin.In this study,antagonistic bacteria with strong inhibitory effect on aflatoxin were screened to provide support for the treatment aflatoxin contamination control in corn.Ten strains which have strong antagonistic effects against A.flavus were isolated from healthy corn from different corn producing areas in China.Among them,the antagonistic bacteria JTFM1001 through corn kernels in vivo and field experiment,the inhibition effect of aflatoxin contamination reached above 70%and 55%,respectively.And the strain was identified as Bacillus subtilis based on its morphological,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA.In addition,our data showed that it can colonize in the rhizosphere and survive for a long time,forming the dominant flora,with broad application prospect.Finally,we were surprised to find that the antibacterial metabolites secreted by the antagonistic bacteria was one of the mechanisms of its inhibition of A.flavus and aflatoxin.This will provide us with new ideas and perspectives on the effective prevention and control of aflatoxin contamination in corn and corn oil.
基金Supported by Technological Department Momentous Item of Heilongjiang Province(GA06C101-07)
文摘It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%, and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%, lower than that of DZW-47. The inhibitory mycelia growth mechanism of different strains on R.solani was quite different, with DZW-3 mainly on the aspect of hyperparasitism, DZW-21 on the synergism of hyperparasitism and metabolite, DZW-47 on the synergism of nutrient competition and secondary metabolite, ZLR-2 and ZLR-11 on producing secondary metabolite. Controlling efficiency of seedling bed accorded basically with that of the broth. The controlling efficiency of DZW-47, ZLR-2, ZLR-11, DZW-21 and DZW-3 were 97.20%, 95.7%, 94.6%, 93.6% and 89.20%, respectively.
基金Supported by National Natural Science Foundation of China(30960010)KeyProject of Principal Fund in Tarim University(TDZKZD06001)~~
文摘[ Objective ] The paper was to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonis- tic effect against Fusarium oxysporum and Verticillium dahliae, and carry out sequence measurement and phylogenetic analysis on 10 representative strains. [ Meth- od] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried out genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and 2 strains with independent groups at Wat- son distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively. [ Conclu- sion ] The genetic diversity of 48 strains of bacteria was obvious. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum and V. dahliae, which could also provide new strain sources for biological control of diseases in cotton production.
文摘Feng Fengyi , Zhang Pin , He Youjian 1, Li Yuhong1 , Zhou Meizhen2 , Chen Gang2 and Li Lin2 The Cancer Hospital of the CAMS & PUMC, Beijing 1000211 The Cancer Hospital of Sun Yat Sen University of Medical Sciences, Guangzhou 5100602Beijing Hospital of the Ministry of Health, Beijing 100730
文摘Aim and methods The study of three-dimensional quantitative structure-activity relationship （3D-QSAR） of DDPH and its derivatives has been performed using Apex-3D programme. Results The result indicates that substituents of para- and ortho-positions in phenyl ring of aryloxyalkylamine greatly influence the bioactivity. Conclusion The biophore model and 3D-QSAR equation help us not only further understand receptor-ligand interactions, but also design new compounds with better bioactivity.
文摘[ Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field. [Method] More than 200 strains of bacteria which could produce spore were isolated from soil in different places. Through screening and rescreening, the bacteria with higher antibacterial activity were conducted observation about thallus shapes and colony characters, a series of physiological and biochemical tests were performed. [Result] Rescreening results indicated that the strains including ZG-10, ZG-19, ZG-59, ZG-72 and ZG-31 had significant antibacterial activity, which had very high research value and good application prospect for biocontrol on spot blotch disease in pakchoi; the strain ZG-10 was identified to be Bacillus subtilis. [ Conclusion] The strain ZG-10 had biocontrol potential and good development prospect. This research laid certain basis for subsequent research and strated a new way for the application of antagonistic strain and proteinum polypeptide in agdculture.
